Anderson, Peter - Flinders University - School of Biological Sciences

研究领域

Biochemical properties of plant disease resistance (R) proteins. Plant R proteins function to recognise invading phytopathogenic disease agents and respond by triggering a signalling pathway that leads to the activation of a resistance response, one such response being localised hypersensitive cell death. This laboratory uses the yeast, pichia pastoris, to manufacture recombinant R protein that is then purified by conventional chromatograhic techniques. The R proteins of interest are those that confer resistance in flax to the flax rust fungus via their recognition of flax rust effector proteins that are secreted into flax cells as part of the disease cycle. We are currently investigating the biochemical properties of purified flax R proteins and focusing specifically on the nucleotide binding and hydrolysis activities of the putative nucleotide binding pocket of the protein. Other projects in the laboratory involve 1) the recombinant production and purification of aspartic proteases from Botrytis cinerea and their use in controlling haze in white wine 2) the over-expression of sucrose transporters in rice grains and the effect on proteins and micronutrient levels.

近期论文

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Bernoux, M., Burdett, H.L., Williams, S.J., Zhang, X., Chen, C., Newell, K., et al. (2016). Comparative Analysis of the Flax Immune Receptors L6 and L7 Suggests an Equilibrium-Based Switch Activation Model. Plant Cell, 28(1) pp. 146-159. [Scopus] Shahsavari, E., Adetutu, E., Anderson, P. and Ball, A. (2013). Necrophytoremediation of phenanthrene and pyrene in contaminated soil. Journal of Environmental Management, 122 pp. 105-112. [10.1016/j.jenvman.2013.02.050] [Scopus] Shahsavari, E., Adetutu, E., Anderson, P. and Ball, A. (2013). Plant residues - A low cost, effective bioremediation treatment for petrogenic hydrocarbon-contaminated soil. Science of The Total Environment, 443 pp. 766-774. [10.1016/j.scitotenv.2012.11.029] [Scopus] Shahsavari, E., Adetutu, E., Anderson, P. and Ball, A. (2013). Tolerance of Selected Plant Species to Petrogenic Hydrocarbons and Effect of Plant Rhizosphere on the Microbial Removal of Hydrocarbons in Contaminated Soil. Water Air and Soil Pollution, 224(4) pp. 1495. [10.1007/s11270-013-1495-3] [Scopus] Koerber, G.R., Anderson, P.A. and Seekamp, J.V. (2013). Morphology, physiology and AFLP markers validate that green box is a hybrid of Eucalyptus largiflorens and E. gracilis (Myrtaceae) Australian Systematic Botany, 26 pp. 156-166. [10.1071/SB12034] [Scopus] Van Sluyter, S., Warnock, N., Schmidt, S., Anderson, P., Van Kan, J., Bacic, A., et al. (2013). Aspartic Acid Protease from Botrytis cinerea Removes Haze-Forming Proteins during White Winemaking. Journal of Agricultural and Food Chemistry, 61(40) pp. 9705-9711. [10.1021/jf402762k] [Scopus] [Web Link] ve, T., Williams, S., Catanzariti, A.M., Rafiqi, M., Rahman, M.M., Ellis, J., et al. (2013). Structures of the flax-rust effector AvrM reveal insights into the molecular basis of plant-cell entry and effector-triggered immunity. Proceedings of the National Academy of Sciences of the United States of America, 110(43) pp. 17594-17599. [10.1073/pnas.1307614110] [Scopus] Koerber, G.R., Seekamp, J., Anderson, P., Whalen, M. and Tyerman, S. (2012). A putative hybrid of Eucalyptus largiflorens growing on salt- and drought-affected floodplains has reduced specific leaf area and leaf nitrogen. Australian Journal of Botany, 60(4) pp. 358-367. [10.1071/BT12012] [Scopus] Holt, S., Cordente, A.G., Williams, S.J., Capone, D., Jitjaroen, W., Menz, R.I., et al. (2011). Engineering Saccharomyces cerevisiae to release 3-Mercaptohexan-1-ol during fermentation through overexpression of an S. cerevisiae gene, STR3 for improvement of wine aroma. Applied and Environmental Microbiology, 77(11) pp. 3626-3632. [10.1128/AEM.03009-10] [Scopus] Ve, T., Williams, S., Stamp, A., Valkov, E., Dodds, P., Anderson, P., et al. (2011). Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 67(12) pp. 1603-1607. [10.1107/S1744309111037675] [Scopus] Williams, S., Sornaraj, P., Decourcey-Ireland, E., Menz, R., Kobe, B., Ellis, J., et al. (2011). An autoactive mutant of the M flax rust resistance protein has a preference for binding ATP, whereas wild-type M protein binds ADP. Molecular Plant-Microbe Interactions, 24(8) pp. 897-906. [10.1094/MPMI-03-11-0052] [Scopus] Lawrence, G.J., Anderson, P.A., Dodds, P.N. and Ellis, J. (2010). Relationships between rust resistance genes at the M locus in flax. Molecular Plant Pathology, 11(1) pp. 19-32. [10.1111/j.1364-3703.2009.00563.x] [Scopus] Simons, K.L., Thomas, S.M. and Anderson, P.A. (2010). Identification of Vibrio natriegens uvrA and uvrB genes and analysis of genes regulation using transcriptional reporter plasmids. Journal of Microbiology, 48(5) pp. 644-656. [10.1007/s12275-010-9370-z] [Scopus] Schmidt, S.A., Tan, E.L., Brown, S., Nasution, U.J., Pettolino, F., Macintyre, O.J., et al. (2009). Hpf2 Glycan Structure Is Critical for Protection against Protein Haze Formation in White Wine. Journal of Agricultural and Food Chemistry, 57 pp. 3308-3315. [10.1021/jf803254s] [Scopus] Schmidt, S.A., Lombardi, M., Gardiner, D., Ayliffe, M. and Anderson, P.A. (2007). The M flax rust resistance pre-mRNA is alternatively spliced and contains a complex upstream untranslated region. Theoretical and Applied Genetics, 115(3) pp. 373-382. [10.1007/s00122-007-0571-8] [Scopus] Schmidt, S.A., Williams, S.J., Wang, C.I.A., Sornaraj, P., James, B., Kobe, B., et al. (2007). Purification of the M flax-rust resistance protein expressed in pichia pastoris. The Plant Journal, 50(6) pp. 1107-1117. [10.1111/j.1365-313X.2007.03104.x] [10.1111/j.1365-313x.2007.03104.x] [Scopus] Forgan, A.H., Knogge, W. and Anderson, P.A. (2007). Asexual genetic exchange in the barley pathogen rhynchosporium secalis. Phytopathology, 97(5) pp. 650-654. [10.1094/PHYTO-97-5-0650] [Scopus] Wang, C.I.A., Guncar, G., Forwood, J.K., Teh, T., Catanzariti, A.M., Lawrence, G.J., et al. (2007). Crystal structure of Flax Rust Avirulence Proteins AvrL567-A and -D reveals details of the structural basis for flax disease resistance specificity. Plant Cell, 19(9) pp. 2898-2912. [10.1105/tpc.107.053611] [Scopus]