个人简介

2010-present Grace Rupley Professor of Chemistry and Biochemistry, University of Notre Dame 2001-2010 Endowed Professor of Analytical Chemistry, University of Washington 1991-2000 Professor, University of Alberta 1986-1991 Associate Professor, University of Alberta 1982-1986 Assistant Professor, University of Wyoming 1980-1982 Postdoctoral Fellow, Los Alamos Scientific Laboratory 1980 Ph.D., in Physical-Analytical Chemistry, University of Utah 1976 B.S. in Chemistry and Mathematics, Northern Illinois University Award: 2012 Fellow, Royal Society of Chemistry 2012 Robert Boyle Award, Royal Society of Chemistry 2012 Fellow, American Association for the Advancement of Science 2012 Chemistry Department Alumni Award, University of Utah 2010-2012 Inaugural class, College of Reviewers, National Institutes of Health 2009 Department of Chemistry Distinguished Alumni Award, Northern Illinois University

研究领域

Biochemistry

The Human Genome Project was the most ambitious research project in the history of analytical chemistry. The goal of that project was the determination of the primary structure of the human genome, which is a group of molecules with a combined molecular weight of 3 billion kilograms. Capillary array electrophoresis with laser induced fluorescence detection in a sheath flow cuvette was the primary analytical instrument used in sequencing the genome; that instrumentation was developed, in part, by the Dovichi research group. Now that the genome is sequenced, interest is focused on the study of the proteome, which is the protein content of an organism, tissue, or cell. The Dovichi group is developing tools to study the proteome with two-dimensional capillary electrophoresis, coupled to either laser-induced fluorescence or mass spectrometry for detection. The group's long-term goal is to study protein expression in single cells and to determine how protein expression changes across a cellular population during cancer progression and during the development of an embryo. In the shorter term, we are developing tools to characterize post-translational modifications of proteins and to speed digestion and analysis of proteins. Proteomics provides a parts list for a cell; it does not necessarily describe how those parts function. Ultimately, the characterization of a cells metabolism, which describes the production and consumption of small molecules, is necessary to understand health and disease. The Dovichi group also is studying metabolic pathways in single cells. In general, a fluorescent substrate is synthesized. Any enzymatic transformation of the substrate can be monitored with exquisite sensitivity as long as the fluorescent label is preserved. We are collaborating with Ole Hindsgaul and Monica Palcic of the Carlsberg Institute and Ron Schnaar at Johns Hopkins University to characterize sphingolipid metabolism in single cells. These glycolipids make up a very large fraction of neuronal membrane, and defects in their metabolism leads to devastating genetic diseases.

近期论文

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Sun, L.L.; Hebert, A.S.; Yan, X.J.; Zhao, Y. M.; Westphall, M.S.; Rush, M.J.P.; Zhu, G.J.; Champion, M.M.; Coon, J.J.; Dovichi, N.J. "Over 10000 Peptide Identifications from the HeLa Proteome by Using Single-Shot Capillary Zone Electrophoresis Combined with Tandem Mass Spectrometry." Angew. Chem. Int. Edit. 2014, 53 (50), 13931-13933. Huge, B.J.; Flaherty, R.J.; Dada, O.O. Dovichi, N.J. "Capillary electrophoresis coupled with automated fraction collection." Talanta 2014, 130, 288-293. Sun, L.L.; Zhu, G.J.; Mou, S.; Zhao, Y.M.; Champion, M.M.; Dovichi, N.J. "Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for quantitative parallel reaction monitoring of peptide abundance and single-shot proteomic analysis of a human cell line." J. Chromatogr. A 2014, 1359, 303-308. Zhu, G.J.; Sun, L.L.; Yan, X.J.; Dovichi, N.J. "Bottom-Up Proteomics of Escherichia coli Using Dynamic pH Junction Preconcentration and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry." Anal. Chem. 2014, 86 (13), 6331-6336. Zhao, Y.M.; Sun, L.L.; Champion, M.M.; Knierman, M.D.; Dovichi, N.J. "Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry for Top-Down Characterization of the Mycobacterium marinum Secretome." Anal. Chem. 2014, 86 (10), 4873-4878. Zhu, G.J.; Sun, L.L.; Linkous, T.; Kernaghan, D.; McGivney, J.B.; Dovichi, N.J. "Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS system." Electrophoresis 2014, 35 (10), 1448-1452.