个人简介

B.S., 1976, Harvey Mudd College Ph.D., 1984,The Pennsylvania State Univ. Director, 1984-1998, Facility for Advanced Instrumentation, Univ. of California, Davis Senior Scientist, 1998-2005, Dept. of Chemistry, The Pennsylvania State Univ. AwardOrganizationDivisionLevel CodeType CodeStart DateEnd Date CNS Distinguished Faculty AwardProfessionalHonors2012

研究领域

Analytical Biological

(Research Description PDF - 837 kb) Global profiling of metabolites, the small molecules produced by living things, provides one of the most powerful strategies for learning about gene and protein functions. This approach, known as metabolomics, is generating information that will serve as the foundation for engineering of plants and microbes to produce renewable feedstocks for high-value bioactive chemicals and biofuels. Metabolite profiles also are important indicators of health and disease, and many metabolites serve important signaling functions that regulate physiological states ranging from inflammation to resolution of tissue damage. Schematic of UHPLC-TOF MS analysis using multiplexed collision induced dissociation showing time-dependence of collision potential. Research in the Jones laboratory is driven by a desire to understand how genetics and environment combine to influence biological chemistry by: (1) developing analytical and biochemical tools for deep profiling and spatial localization of specialized metabolites that accumulate in plant cells, (2) developing experimental and data mining approaches to accelerate discoveries of natural products in medicinal plants and the genes involved in their accumulation, (3) deployment of mass spectrometry strategies to identify and quantify by-products generated during processing of lignocellulosic biomass for conversion into renewable fuels and chemicals, and (4) measurements of metabolic biomarkers of inflammation, analgesia, and resolution of disease in human subjects with chronic inflammatory diseases. Laser desorption ionization mass spectrometry imaging of two insecticidal acylsugar metabolites in leaf trichomes of the wild tomato relativeSolanum habrochaites LA1777. Many plants accumulate large quantities of bioactive phytochemicals in specialized epidermal cells known as glandular trichomes, which are prolific biochemical factories. Our laboratory has pioneered rapid metabolite profiling protocols based on ultrahigh performance liquid chromatography (UHPLC) coupled to time-of-flight mass spectrometry. By employing rapid gradients and by multiplexing collision potentials across a lens between the mass spectrometer ion source and mass analyzer, ~500 metabolites are measured in a 5-minute analysis. This allows for large-scale screening of genetic variants to guide gene function discoveries. Trichomes are also amenable to mass spectrometry imaging, allowing for extensive chemical interrogation of individual trichomes across plant tissues. Ongoing research involves labeling trichome metabolites using 13C to investigate metabolic dynamics coupled with elucidation of metabolite structures using multidimensional NMR on the 900 MHz NMR spectrometer at the Max T. Rogers NMR Facility. We are also investigating whether inflammatory, anti-inflammatory, and analgesic lipid metabolite biomarkers in the blood of COPD patients are reliable indicators of the mechanisms underlying osteopathic manipulative treatment (OMT). We are also investigating whether inflammatory, anti-inflammatory, and analgesic lipid metabolite biomarkers in the blood of COPD patients are reliable indicators of the mechanisms underlying osteopathic manipulative treatment (OMT). .

近期论文

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Identification of methylated flavonoid regioisomeric metabolites using enzymatic semisynthesis and liquid chromatography-tandem mass spectrometry, C. Li, A. Schmidt, E. Pichersky, F. Shi, and A. D. Jones, Metabolomics 2013, 9, S92-S101. Profiling of soluble oligosaccharides from treated biomass using solid phase extraction and LC-TOF MS, R. Vismeh, J. F. Humpula, S. C. P. Chundawat, V. Balan, B. E. Dale, and A. D. Jones, Carbohydrate Polymers 2013, 94, 791-799. Plastochromanol-8 and tocopherols are essential lipid-soluble antioxidants during seed dessication and quiescence in Arabidopsis, L. Méne-Saffrané, A. D. Jones, and D. DellaPenna, Proceedings of the National Academy of Sciences of the United States of America 2010, 107: 17815-17820. Broad connections in the Arabidopsis seed metabolic network revealed by metabolite profiling of an amino acid catabolism mutant, L. Gu, A. D. Jones, and R. L. Last, Plant Journal 2010, 61, 579-590. Mass spectrometry screening reveals widespread diversity in trichome specialized metabolites of tomato chromosomal substitution lines, A. Schilmiller, F. Shi, J. Kim, A. Charbonneau, D. Holmes, A. D. Jones, and R. L. Last, Plant Journal, 2010, 62, 391-403. A rapid wound signal activates systemic synthesis of bioactive jasmonates in Arabidopsis, A. J. K. Koo, X. Gao, A. D. Jones, and G. A. Howe, Plant Journal 2009, 59, 974-986. Towards the plant metabolome and beyond, R. L. Last, A. D. Jones, and Y. Shachar-Hill, Nature Reviews Molecular Cell Biology 2007, 8, 167-174.