Fussenegger, Martin - Eidgenössische Technische Hochschule Zürich - Department of Chemistry and Applied Biosciences

个人简介

Martin Fussenegger has been Swiss Science Foundation Professor for Molecular Biotechnology at the Institute of Biotechnology at ETH Zurich, since September 1st, 2002. Since October 1st, 2004 he is Associate Professor and since February full Professor at the Institute for Chemical and Bioengineering. Since April 2008 he is professor at the Department of Biosystems Science and Engineering (D-BSSE).

近期论文

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Identification of a novel proliferation-inducing determinant using lentiviral expression cloning Chilov, Dmitri, Fux, Cornelia, Joch, Hana and Fussenegger, Martin (2003) One of the major challenges in the post-genome era is the correlation between genes and function or phenotype. We have pioneered a strategy for screening of cDNA libraries, which is based on sequential combination of lentiviral and oncoretroviral expression systems and can be used... Novel macrolide-adjustable bidirectional expression modules for coordinated expression of two different transgenes in mice Baessler, Cornelia, Weber, Wilfried, Baba, Marie Daoud-El, Heinzen, Mareike, Aubel, Dominique and Fussenegger, Martin (2003) Background: Precise control of transgene expression is essential for a variety of applications ranging from gene-function analysis, biopharmaceutical manufacturing to next-generation molecular interventions in gene therapy and tissue engineering. The regulation of gene expression... Toward higher order control modalities in mammalian cells Fux, Cornelia and Fussenegger, Martin (2003) Heterologous higher order control modalities will be important tools for targeted multigene interventions in next-generation gene therapy, tissue engineering, and sophisticated gene-function studies. In this study, we present the design and rigorous quantitative analysis of a... Metabolic engineering of mammalian cells for higher protein yield Kaufmann, Hitto and Fussenegger, Martin (2003) Pharmaceutical Biotechnology (2nd Edition) Fussenegger, Martin (2003) Modulation of translation-initiation in CHO-K1 cells by rapamycin-induced heterodimerization of engineered eIF4G fusion proteins Schlatter, Stefan, Senn, Claudia and Fussenegger, Martin (2003) Translation-initiation is a predominant checkpoint in mammalian cells which controls protein synthesis and fine-tunes the flow of information from gene to protein. In eukaryotes, translation-initiation is typically initiated at a 7-methyl-guanylic acid cap posttranscriptionally... Novel CNBP- and La-based translation control systems for mammalian cells Schlatter, Stefan and Fussenegger, Martin (2003) Throughout the development of Xenopus, production of ribosomal proteins (rp) is regulated at the translational level. Translation control is mediated by a terminal oligopyrimidine element (TOP) present in the 5' untranslated region (UTR) of rp-encoding mRNAs. TOP elements adopt a... Conditional human VEGF‐mediated vascularization in chicken embryos using a novel temperature‐inducible gene regulation (TIGR) system Weber, Wilfried, Marty, René R., Link, Nils, Ehrbar, Martin, Keller, Bettina, Weber, Cornelia C., Zisch, Andreas H., Heinzen, Christoph, Djonov, Valentin and Fussenegger, Martin (2003) Advanced heterologous transcription control systems for adjusting desired transgene expression are essential for gene function assignments, drug discovery, manufacturing of difficult to produce protein pharmaceuticals and precise dosing of gene‐based therapeutic interventions.... Streptomyces-derived quorum-sensing systems engineered for adjustable transgene expression in mammalian cells and mice Weber, Wilfried, Schoenmakers, Ronald, Spielmann, Manuela, Baba, Marie Daoud-El, Folcher, Marc, Keller, Bettina, Weber, Cornelia C., Link, Nils, van de Wetering, Petra, Heinzen, Christoph, Jolivet, Benoit, Séquin, Urs, Aubel, Dominique, Thompson, Charles J. and Fussenegger, Martin (2003) Prokaryotic transcriptional regulatory elements have been adopted for controlled expression of cloned genes in mammalian cells and animals, the cornerstone for gene-function correlations, drug discovery, biopharmaceutical manufacturing as well as advanced gene therapy and tissue... Modulation of translation-initiation in CHO-K1 cells by rapamycin-induced heterodimerization of engineered eIF4G fusion proteins Schlatter, Stefan, Senn, Claudia and Fussenegger, Martin (2003) Translation-initiation is a predominant checkpoint in mammalian cells which controls protein synthesis and fine-tunes the flow of information from gene to protein. In eukaryotes, translation-initiation is typically initiated at a 7-methyl-guanylic acid cap posttranscriptionally... Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types Kelm, Jens M., Timmins, Nicholas E., Brown, Catherine J., Fussenegger, Martin and Nielsen, Lars K. (2003) Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent surfaces, in suspension, or on scaffolds, have a number of drawbacks, including the need for manual... Artificial regulatory networks and cascades for discrete multilevel transgene control in mammalian cells Kramer, Beat P., Weber, Wilfried and Fussenegger, Martin (2003) 'Prototype drug-adjustable heterologous transcription control systems designed for gene therapy applications typically show sigmoid dose-response characteristics and enable fine-tuning of therapeutic transgenes only within a narrow inducer concentration range of a few nanograms.... Bidirectional expression units enable streptogramin-adjustable gene expression in mammalian cells Fux, Cornelia and Fussenegger, Martin (2003) Serious initiatives in gene therapy and tissue engineering require a sophisticated molecular toolbox combining DNA transfer technologies, human-compatible transcription control systems, as well as compact and robust expression configurations. We have designed several versatile... Design and in vivo characterization of self-inactivating human and non-human lentiviral expression vectors engineered for streptogramin-adjustable transgene expression Mitta, Barbara, Weber, Cornelia C., Rimann, Markus and Fussenegger, Martin (2004) Adjustable transgene expression is considered key for next-generation molecular interventions in gene therapy scenarios, therapeutic reprogramming of clinical cell phenotypes for tissue engineering and sophisticated gene-function analyses in the post-genomic era. We have designed a... Dual-regulated expression of C/EBP-alpha and BMP-2 enables differential differentiation of C2C12 cells into adipocytes and osteoblasts Fux, Cornelia, Mitta, Barbara, Kramer, Beat P. and Fussenegger, Martin (2004) CCAAT/enhancer-binding proteins (C/EBPs) as well as bone morphogenic proteins (BMPs) play essential roles in mammalian cell differentiation in shaping adipogenic and osteoblastic lineages in particular. Recent evidence suggested that adipocytes and osteoblasts share a common... Inducible Gene Expression in Mammalian Cells and Mice Weber, Wilfried and Fussenegger, Martin (2004) Inducible expression of desired transgenes in mammalian cells and animals is a current priority in basic and applied research, biopharmaceutical manufacturing, gene therapy, and tissue engineering, as well as in drug discovery. Among the most prominent human-compatible transgene... Synergies of microtissue design, viral transduction and adjustable transgene expression for regenerative medicine Kelm, Jens M., Kramer, Beat P., Gonzalez-Nicolini, Valeria, Ley, Bettina and Fussenegger, Martin (2004) In the past decade, regenerative medicine has evolved as an interdisciplinary field, integrating expertise from the medical, life- and material-science communities. Recent advances in tissue engineering, gene therapy, gene-function analysis, animal-free drug testing, drug... New-generation multicistronic expression platform Fux, Cornelia, Langer, Dominik, Kelm, Jens M., Weber, Wilfried and Fussenegger, Martin (2004) Capitalizing on a proven multicistronic expression vector platform we have designed novel pTRIDENT vectors which (1) enable coordinated expression of three desired transgenes, (2) are size-optimized, (3) take advantage of small highly efficient internal ribosome entry sites of the... Microscale tissue engineering using gravity-enforced cell assembly Kelm, Jens M. and Fussenegger, Martin (2004) Designing artificial microtissues by reaggregation of monodispersed primary cells, neoplastic or engineered cell lines is providing insight into cell-cell interactions and underlying regulatory networks. Recent advances in microtissue production have highlighted the potential of... Modeling the quorum sensing regulatory network of human-pathogenic Pseudomonas aeruginosa Viretta, Alessandro Usseglio and Fussenegger, Martin (2004) The biochemical network underlying quorum sensing in human-pathogenic Pseudomonas aeruginosa is one of the best characterized. Mathematical modeling is required to untangle the complexity of its architecture and dynamics. We present a qualitative model of the P. aeruginosa...