个人简介
学习与工作经历:
2014—2018 吉林大学动物科学学院 教授
2014—2018 吉林大学动物科学学院 副教授
2012—2014年,吉林大学动物科学学院 讲师
2006—2012年,吉林大学 动物学 博士
2009—2011年,UW-Madison 医学院 联合培养博士
2002—2006年,吉林大学 动物科学 学士
主要教学工作(承担的本科生、研究生课程)
本科生课程:《发育生物学》、《动物胚胎工程》。
研究生课程:《分子遗传学》、《动物胚胎工程》
主要科研工作(包括科研项目)
长期致力于疾病动物模型的构建及其在生物医药中的应用,利用CRISPR/Cas9 基因编辑技术,已成功构建20余种疾病模型兔,10余种疾病模型猪。以通讯作者或第一作者,在Nature Communication, Genome Biology, Hum Mol Genet、Cell Mol Life Sci、Disease Models & Mechanisms及Aging and Disease等领域内杂志发表相关SCI论文30余篇。
承担科研项目
1.神经疾病大动物模型的建立及干细胞治疗评价,国家重点研发计划,697万元,2017.07-2021.12,课题负责人
2.猪孤雌胎儿发育阻滞的影响因素研究,国家自然基金,24万元,2013.01-2015.12,项目负责人
3.广谱抗病毒DRACO转基因猪新品种培育,转基因专项,54.6万元,2014.01-2017.12,课题负责人
4.利用CRISPR/Cas9构建用于HIV-1研究的转基因兔模型,博士后特别资助,2014.07-2015.07,15万元,项目负责人
5.广谱抗病毒转基因猪的制备,博士后面上资助(一等),2013.09-2015.05,8万元,项目负责人
奖励与荣誉
李占军(3/10), 猪克隆与基因修饰技术创新及其在生物医学模型中的应用, 吉林省科技厅, 自然科学, 省部一等奖, 2017.11.10 (赖良学,李小平,李占军,欧阳红生,邓旭明,逄大欣,信吉阁,黄永业,周小青,黄娇).
近期论文
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(1) Tingting Sui, Yuning Song, Zhiquan Liu, Mao Chen, Jichao Deng, Yuanyuan Xu, Liangxue Lai*, Zhanjun Li*, CRISPR-induced exon skipping is dependent on premature termination codon mutations, Genome Biology, GBIO-D-18-00763.
(2) Zhiquan Liu, Mao Chen, Dr. Siyu Chen, Jichao Deng , Yuning Song , Liangxue Lai*, Zhanjun Li*, Highly efficient RNA-guided base editing in rabbit, Nature Communications. 2018 Jul 13;9(1):2717.
(3) Zhiquan Liu, Mao Chen, Huanhuan Shan, Siyu Chen, Yuxin Xu, Yuning Song, Quanjun Zhang, Hongsheng Ouyang, Zhanjun Li*, Liangxue Lai*. Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7). Cell Mol Life Sci. 2019 Apr 27.
(4) Tingjun Liu, Jun Wang, Xudong Xie, Ke Wang, Tingting Sui, Di Liu, Liangxue Lai, Hu Zhao, Zhanjun Li*, and Jian Q Feng*. 2DMP1 Ablation in the Rabbit Results in Mineralization Defects and Abnormalities in Haversian Canal/Osteon Microarchitecture. J Bone Miner Res. 2019 Mar 3.封面文章.
(5) Jichao Deng, Mao Chen, Zhiquan Liu, Yuning Song, Tingting Sui, Liangxue Lai, Zhanjun Li* The disrupted balance between hair follicles and sebaceous glands in Hoxc13-ablated rabbits. FASEB Journal. 2018 Aug 20:fj201800928RR.
(6) Tingting Sui, Li Xu, Yeh Siang Lau, Di Liu, Tingjun Liu, Yandi Gao, Liangxue Lai, Renzhi Han, Zhanjun Li*.Development of muscular dystrophy in a CRISPR-engineered mutant rabbit model with frame-disrupting ANO5 mutations. Cell Death Dis. 2018 May 22;9 (6):609.
(7) Tingting Sui#, Di Liu#, Tingjun Liu, Jichao Deng, Mao Chen, Yuanyuan Xu, Yuning Song, Hongsheng Ouyang, Liangxue Lai*, Zhanjun Li*, LMNA-mutated Rabbits: A Model of Premature Aging Syndrome with Muscular Dystrophy and Dilated Cardiomyopathy, Aging Dis, 2018.2.16, 9(6).
(8) Tingting Sui#, Lin Yuan#, Huan Liu, Mao Chen, Jichao Deng, Yong Wang, Zhanjun Li*, Liangxue Lai*, CRISPR/Cas9-mediated mutation of PHEX in rabbit recapitulates human X-linked hypophosphatemia (XLH), Hum Mol Genet, 2016, 25(13): 2661-2671.
(9) Yuning Song#, Lin Yuan#, Yong Wang, Mao Chen, Jichao Deng, Qingyan Lv, Tingting Sui, Zhanjun Li*, Liangxue Lai*, Efficient dual sgRNA-directed large gene deletion in rabbit with CRISPR/Cas9 system, Cell Mol Life Sci, 2016, 73(15):2959-2968.
(10) Lin Yuan#, Tingting Sui#, Mao Chen, Jichao Deng, Yongye Huang, Jian Zeng, Qingyan Lv, Yuning Song, Li Zhanjun*, Liangxue Lai*, CRISPR/Cas9-mediated GJA8 knockout in rabbits recapitulates human congenital cataracts, Sci Rep, 2016, 6:22024.
(11) Tingting Sui, Yeh Siang Lau, Di Liu, Tingjun Liu, Li Xu, Yandi Gao, Liangxue Lai, Zhanjun Li*, Renzhi Han*, A novel rabbit model of Duchenne muscular dystrophy generated by CRISPR/Cas9. Dis Mod Mech, 2018 Jun 4;11(6).
(12) Mao Chen#, Bin Yao, Qiang Bin, Jichao Deng, Yuning Song, Tingting Sui, Lina Zhou, Haobin Yao, Yuanyuan Xu, Daxin Pang, Hongsheng Ouyang, Zhanjun Li*, Liangxue Lai*, Truncated C-terminus of fibrillin-1 induces Marfanoid-progeroid-lipodystrophy (MPL) syndrome in rabbit, Dis Mod Mech, 2018.03.07, 03(15).
(13) Qingyan Lv#, Lin Yuan#, Yuning Song, Tingting Sui, Zhanjun Li*, Liangxue Lai*, D-repeat in the XIST gene is required for X chromosome inactivation, RNA Biol, 2016, 13(2):172-176.