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2020．
1）Wang,W.,Yang,J.,Sun,Y.*,Li,Z.*,and You,C.*.(2020)Artificial ATP-free in vitro Synthetic Enzymatic Biosystems Facilitate Aldolase-mediated C-C Bond Formation for Biomanufacturing,ACS Catalysis 10,1264-1271.
2019.
1)Meng,D.,Wu,R.,Wang,J.,Zhu,Z.*,and You,C.*(2019)Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem,Biotechnol.Biofuels 12,267.
2)Li,Y.,Liu,S.,and You,C.*(2019)Permeabilized Escherichia coli Whole Cells Containing Co-Expressed Two Thermophilic Enzymes Facilitate the Synthesis of scyllo-Inositol from myo-Inositol,Biotechnology Journal 0,1900191.
3)Meng,D.,Wang,J.,and You,C.*.(2019)The properties of the linker in a mini-scaffoldin influence the catalytic efficiency of scaffoldin-mediated enzyme complexes,Enzyme and Microbial Technology.10.1016/j.enzmictec.2019.109460
4)Bai,X.,Meng,D.,Wei,X.,Zhou,X.,Lu,F.,and You,C.*.(2019)Facile synthesis of(？)-vibo-quercitol from maltodextrin via an in vitro synthetic enzymatic biosystem,Biotechnol.Bioeng.116,2710-2719.
5)Wang,W.,Meng,D.,Li,Q.,Li,Z.,and You,C.*.(2019)Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem,Bioresour.Bioprocess.6:22
6)Sun,S.,Wei,X.,and You,C.*.(2019)The construction of an in vitro synthetic enzymatic biosystem that facilitates laminaribiose biosynthesis from maltodextrin and glucose,Biotechnol.J.14,1800493.
7)Song,Y.,Liu,M.,Xie,L.,You,C.*.,Sun,J.*,and Zhang,Y.-H.P.J.*(2019)A Recombinant 12-His Tagged Pyrococcus furiosus Soluble[NiFe]-Hydrogenase I Overexpressed in Thermococcus kodakarensis KOD1 Facilitates Hydrogen-Powered in vitro NADH Regeneration,Biotechnol.J.14,1800301.
8)Meng,D.,Liang,A.,Wei,X.,and You,C.*.(2019)Enzymatic characterization of a thermostable phosphatase from Thermomicrobium roseum and its application for biosynthesis of fructose from maltodextrin,Appl Microbiol Biot.103,6129-6139.
9)魏欣蕾，游淳*（2019）外多酶分子机器的现状和最新进展，生物工程学报.35(10):1870？1888
10)Ma,C.,Wu,R.,Huang,R.,Jiang,W.,You,C.,Zhu,L.,and Zhu,Z.(2019)Directed evolution of a 6-phosphogluconate dehydrogenase for operating an enzymatic fuel cell at lowered anodic pHs,Journal of Electroanalytical Chemistry 851.
11)Song,H.,Ma,C.,Liu,P.,You,C..,Lin,J.,and Zhu,Z.(2019)A hybrid CO2 electroreduction system mediated by enzyme-cofactor conjugates coupled with Cu nanoparticle-catalyzed cofactor regeneration,Journal of CO2 Utilization 34,568-575.
12)Zhang,S.,Shi,J.,Sun,Y.,Wu,Y.,Zhang,Y.,Cai,Z.,Chen,Y.,You,C.,Han,P.,and Jiang,Z.(2019)Artificial Thylakoid for the Coordinated Photoenzymatic Reduction of Carbon Dioxide,ACS Catalysis 9,3913-3925.
2018.
13)Meng,D.,Wei,X.,Zhang,Y.-H.P.J.,Zhu,Z.,You,C.*,and Ma,Y.(2018)Stoichiometric conversion of cellulosic biomass by in vitro synthetic enzymatic biosystems for biomanufacturing,ACS Catalysis 8,9550-9559.
14)Wei,XL.,Xie,L.,Zhang,Y.-H.P.J.,and You,C.*(2018)Stoichiometric Regeneration of ATP by A NAD(P)/CoA-free and Phosphate-balanced in Vitro Synthetic Enzymatic Biosystem,ChemcatChem,10,5597-5601.
15)Xie,L.,Wei,X.,Zhou,X.,Meng,D.,Zhou,R.,Zhang,Y.-H.P.J.,Xu,S*.,and You,C.*.(2018)Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions,Synthetic and Systems Biotechnology.3,204-210.
16)Shi,T.,Han,P.,You,C.*,and Zhang,Y.-H.P.J.*(2018)An in vitro synthetic biology platform for emerging
industrial biomanufacturing:Bottom-up pathway design,Synthetic and Systems Biotechnology.3,186-195.
17)Song,H.,Ma,C.,Zhou,W.,You,C..,Zhang,Y.P.J.,and Zhu,Z.(2018)Construction of Enzyme-Cofactor/Mediator Conjugates for Enhanced in Vitro Bioelectricity Generation,Bioconjug Chem 29,3993-3998.
18)Zhu,Z.,You,C..,Ma,Y.,and Zhang,Y.-H.P.J.(2018)In vitro synthetic enzymatic biosystems at the interface of the food-energy-water nexus:A conceptual framework and recent advances,Process Biochem 74,43-49.
2017.
19)You,C.*,Huang R,Zhu ZG,Zhang Y-HP*.2017 Protein engineering on nicotinamide-based coenzymes with its applications to synthetic biology.Synthetic and Systems Biotechnology 2017;2:208-218.
20)You,C.,Shi T,Li YJ,Han PP,Zhou XG,Zhang Y-HP*.2017.An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch.Biotechnology and Bioengineering 114:1855-1864.
21)You,C.*,Zhang Y-HP.2017.Biomanufacturing by in vitro biosystems containing cascade enzymes.Process Biochemistry.52:106-114.
22)Zhong C,You,C.,Wei P,Zhang Y-HP*.2017.Thermal cycling cascade biocatalysis of myo-inositol synthesis from sucrose.ACS Catalysis.7:5992–5999.
2016.
23)Kim JE,Huang R,Chen H,You,C.,Zhang Y-HP.2016.Facile construction of random gene mutagenesis library for directed evolution without the use of restriction enzyme in Escherichia coli.Biotechnol J.11:1142-1150.
24)Zhou W,You,C.,Ma H,Ma Y,Zhang YH.2016.One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes.J Agric Food Chem.64:1777-1783.
2015.
25)Rollin JA,Martin del Campo J,Myung S,Sun F,You,C.,Bakovic A,Castro R,Chandrayan SK,Wu CH,Adams MWW,Senger RS,Zhang Y-HP,2015.High-yield hydrogen production from biomass by in vitro metabolic engineering:Mixed sugars coutilization and kinetic modeling.Proceedings of the National Academy of Sciences.112(16),4964-4969.
2014.
26)You,C.,Zhang Y-HP*.2014.Annexation of a high-activity rate-limiting enzyme in a synthetic three-enzyme complex greatly decreases the degree of substrate channeling.ACS Synthetic Biology,3(6):380-386
27)Zhu ZG,Tam TK,Sun FF,You,C.,Zhang Y-HP*.2014.A high-energy-density sugar biobattery based on a synthetic enzymatic pathway.Nature Communications.5:3026,
28)Myung S,Rollin J,You,C.,Sun F,Chandrayan S,Adams MWW,Zhang Y-HP.2014.In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose.Metabolic Engineering 24(0):70-77.
29)Peng Q,You,C.,Zhang Y-HP*,2014,One-Pot Enzymatic Conversion of Sucrose to Synthetic Amylose by using Enzyme Cascades.ACS Catalysis.4:1311-1317.
30)Gao SH,You,C.,Renneckar S,Bao J,Zhang Y-HP*.2014.New insights into enzymatic hydrolysis of heterogeneous cellulose by using CBM3-containing GFP and CBM17-containing CFP.Biotechnology for Biofuels.7(1):24
2013.
31)You,C.,Chen HG,Myung S,Sathisuksanoh N,Ma H,Zhang XZ,Li JY,Zhang Y-HP*.2013.Enzymatic transformation of non-food biomass to starch.Proceedings of the National Academy of Sciences of the USA.110:7182-7189.(Highlighted by Science magazine)
32)You,C.,Zhang Y-HP*.2013.Self-assembly of synthetic metabolons through synthetic scaffoldins:single-step purification,co-immobilization,and substrate channeling.ACS Synthetic Biology 2:102-110,(cover page).
33)Martin del Campo JS,Rollin JR,Myung S,You,C.,Chandrayan S,Adams MWW,Zhang Y-HP*.2013.Dihydrogen production from xylose and water mediated by synthetic cascade enzymes.Angewandte Chemie International Edition 52:4587-4590,
34)Myung S,You,C.,Zhang Y-HP*.2013.Recyclable cellulose-containing magnetic nanoparticles:immobilization of cellulose-binding module-tagged proteins and synthetic metabolon featuring substrate channeling.Journal of Materials Chemistry B.1:4419-4427.
35)Martin del Campo JS,You,C.,Kim J-E,Zhang Y-HP*.2013.Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima.Journal of Industrial Microbiology and Biotechnology 40:661-669.
2012.
36)You,C.,Myung S,Zhang Y-HP*.2012.Facilitated substrate channeling in a self-assembled trifunctional enzyme complex.Angewandte Chemie International Edition.51:8787-8790
37)You,C.,Zhang X-Z,Sathitsuksanoh N,Lynd LR,Zhang Y-HP*.2012.Enhanced microbial cellulose utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex.Applied and Environmental Microbiology 78(5):1437-1444
38)You,C.,Zhang X-Z,Zhang Y-HP*.2012.Simple Cloning via direct transformation of PCR product(DNA multimer)to Escherichia coli and Bacillus subtilis.Applied and Environmental Microbiology 78(5):1593-1595
39)You,C.,Zhang Y-HP*.2012.Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.Analytical Biochemistry 428:7-12
40)You,C.,Zhang X-Z,Zhang Y-HP*.2012.Mini-scaffoldin enhanced mini-cellulosome hydrolysis performance on low-accessibility cellulose(Avicel)more than on high-accessibility amorphous cellulose.Biochemical Engineering Journal 63:57-65
41)Xue HP,Zhou JG,You,C.,Huang Q,Lu H*.2012.Amino acid substitutions in the N-terminus,cord andα-helix domains improved the thermostability of a family 11 xylanase XynR8.Journal of Industrial Microbiology and Biotechnology 39:1279-1288
42)Zhou JG,Bao L,Chang L,Liu Z,You,C.,Lu H*.2012.Beta-xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans.Letters in Applied Microbiology 54:79-87
2011.
43)Zhang Y-HP*,Myung S,You,C.,Zhu ZG,Rollin J.2011.Toward low-cost biomanufacturing through cell-free synthetic biology:bottom-up design.Journal of Materials Chemistry 21:18877-18886
2010.
44)You,C.,Huang Q,Xue HP,Xu Y,Lu H*.2010.Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum.Biotechnology and Bioengineering 105(5):861-70
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