个人简介
招生专业
070703-海洋生物学
090801-水产养殖
招生方向
繁殖、发育与调控技术
生理生化过程与调控
养殖遗传学与育种生物技术
教育背景
2018-10--2019-11 密歇根大学分子细胞发育生物学系 访问学者
2005-09--2008-07 中国科学院海洋研究所 博士
2002-09--2005-07 中国海洋大学 硕士
1998-09--2002-07 青岛农业大学 本科
工作简历
2018-10~2019-11,密歇根大学分子细胞发育生物学系, 访问学者
2017-01~现在, 中国科学院海洋研究所, 研究员四级
2015-01~2016-12,中国科学院海洋研究所, 副研究员一级
2012-12~2014-12,中国科学院海洋研究所, 副研究员二级
2012-06~现在, 中国科学院海洋研究所, 硕导
2011-01~2012-12,中国科学院海洋研究所, 副研究员三级
2009-12~2010-12,中国科学院海洋研究所, 助理研究员一级
2008-10~2009-12,中国科学院海洋研究所, 助理研究员二级
2008-07~2008-10,中国科学院海洋研究所, 助理研究员三级
2005-09~2008-07,中国科学院海洋研究所, 博士
2002-09~2005-07,中国海洋大学, 硕士
1998-09~2002-07,青岛农业大学 , 本科
教授课程
繁殖发育生物学
繁育生物学
发育生物学
繁殖生理学
奖励信息
(1) 条石鲷生殖调控及规模化生产技术的建立与应用, 二等奖, 省级, 2014
(2) 杂交鲆(牙鲆♀×夏鲆♂)规模化生产技术的建立与应用, 一等奖, 市地级, 2014
(3) 国家海洋局科学技术二等奖, 二等奖, 省级, 2013
专利成果
( 1 ) 一种大菱鲆胚胎发育期原始生殖细胞(PGCs)的定位标记方法, 2016, 第 1 作者, 专利号: 201310274379.0
( 2 ) 一种夏鲆冻精与牙鲆种间杂交人工授精方法, 2015, 第 1 作者, 专利号: 201310212970.3
( 3 ) 一种皱纹盘鲍优质精子诱导和采集方法, 2016, 第 1 作者, 专利号: 201310517084.1
( 4 ) 一种星斑川鲽精子超低温冷冻保存方法, 2014, 第 1 作者, 专利号: ZL201310213169.0
( 5 ) 一种夏鲆冻精与牙鲆种间杂交人工授精的方法, 2014, 第 1 作者, 专利号: ZL201310212970.3
( 6 ) 一种夏鲆精子便捷式超低温冷冻保存方法, 2014, 第 1 作者, 专利号: ZL201310214338.2
( 7 ) 太平洋鳕鱼精子高效率超低温冷冻保存方法, 2015, 第 1 作者, 专利号: ZL201310214264.2
( 8 ) 一种太平洋牡蛎胚胎超低温冷冻保存方法, 2016, 第 1 作者, 专利号: ZL201410004766.7
( 9 ) 一种褐牙鲆与其近缘外来物种种质间的pcr鉴定方法, 2017, 第 5 作者, 专利号: ZL 201310246976.2
( 10 ) 一种卵胎生鱼类许氏鲆鲉精子超低温保存及激活方法, 2019, 第 1 作者, 专利号: 201910166977 .3
发表著作
( 1 ) 鱼类精子和胚胎冷冻保存理论与技术, Theory and techonology of cryopreservation of fish sperm and embryos, 中国农业出版社, 2007-07, 第 2 作者
( 2 ) 海水鱼类精子冷冻保存和精子结构功能质量评价, Marine fish sperm cryopreservation and quality evaluation in sperm structure and function. Cryopreservation, InTech, 2012-03, 第 1 作者
科研项目
( 1 ) 超低温状态下活性氧对鱼类精子的损伤机制及其调控研究, 主持, 国家级, 2011-01--2013-12
( 2 ) 牙鲆与夏鲆杂交及回交分子细胞遗传学机理研究, 主持, 省级, 2010-01--2012-12
( 3 ) 超低温保存对海水鱼类精子质量的影响, 主持, 部委级, 2010-01--2012-12
( 4 ) 海水鱼类原始生殖细胞生成模式及其分离和冷冻保存研究, 主持, 省级, 2012-01--2014-09
( 5 ) 重要海水鱼类精子冷冻保存技术及种质库的建立, 主持, 市地级, 2012-01--2013-12
( 6 ) 鲆鲽类现代产业技术体系建设, 参与, 国家级, 2011-01--2015-12
( 7 ) 高温诱导牙鲆原始生殖细胞迁移增殖及和雄性化机制研究, 主持, 国家级, 2014-01--2017-12
( 8 ) 中国科学院青年促进会项目, 主持, 部委级, 2014-01--2017-12
( 9 ) 海水鱼类原始生殖细胞生成模式及其分离和冷冻保存研究, 主持, 省级, 2012-01--2014-09
( 10 ) “十二五”国家863计划“海洋生物细胞分子育种关键技术”, 参与, 国家级, 2012-01--2015-12
( 11 ) 海水养殖种质改良技术和产业化示范, 参与, 部委级, 2012-01--2014-12
( 12 ) 鲆鲽鱼类精子成熟及其超活化获能机制研究, 主持, 国家级, 2016-01--2019-12
( 13 ) 大西洋鲑鱼种高效扩繁技术工艺与示范, 参与, 省级, 2018-01--2020-12
( 14 ) 重要养殖鱼类生殖干细胞诱导与移植技术, 主持, 国家级, 2018-12--2022-12
( 15 ) 海水鱼类精原干细胞移植即“借腹生子技术”研发, 主持, 省级, 2019-01--2020-12
( 16 ) 蓝色生命前前技术, 参与, 省级, 2019-01--2020-12
参与会议
(1)基于vasa/dnd基因标记的两种海水鱼原始生殖细胞发生发育的究 基于vasa/dnd基因标记的两种海水鱼原始生殖细胞发生发育的究 刘清华 林帆 李军 2013-11-01
(2)Marine fish genetic resources cryoperservation and application in breeding 刘清华 2013-08-23
近期论文
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(1) Sequencing of the black rockfish chromosomal genome provides insight into sperm storage in the female ovary, DNA Research, 2019, 第 1 作者
(2) High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus), General and Comparative Endocrinology, 2019, 通讯作者
(3) Skeletal ontogeny and deformity during the early fry culture process for Epinephelus lanceolatus, Aquaculture, 2019, 通讯作者
(4) Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3’ Untranslated Region of nanos3 or vasa Gene, Marine Biotechnology, 2019, 通讯作者
(5) High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus), General and Comparative Endocrinology, 2019, 通讯作者
(6) Skeletal ontogeny and deformity during the early fry culture process for Epinephelus lanceolatus, Aquaculture, 2019, 通讯作者
(7) Identificatio n of type A spermatogonia in turbot (Scophthalmus maximus) using a new stem cell specific antigen 75 (ly75/CD205), Chinese Journal of Oceanology and Limnology, 2018, 通讯作者
(8) Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors, Fish physical Biochem, 2018, 通讯作者
(9) Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All-Female Japanese Flounder (Paralichthys Olivaceus), THE ANATOMICAL RECORD, 2018, 通讯作者
(10) Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation., Scientific reports, 2018, 通讯作者
(11) Osteological ontogeny and allometric growth in larval and juvenile turbot (Scophthalmus maximus)., Aquaculture, 2018, 通讯作者
(12) Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus)., Comparative Biochemistry and Physiology, Part B, 2018, 通讯作者
(13) Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus)., Theriogenology., 2018, 通讯作者
(14) High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention, Aquaculture, 2017, 通讯作者
(15) Gonadogenesis analysis and sex differentiation in cultured turbot (Scophthalmus maximus), Fish Physiol Biochem, 2017, 通讯作者
(16) The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P. olivaceus P. dentatus ) to chronic and acute heat stress., Marine Environmental Research, 2017, 通讯作者
(17) Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus., Chinese Journal of Oceanology and Limnology,, 2017, 通讯作者
(18) Sperm cryopreservation in different grouper shuspecies and application in interspecifid hybridization., Theriogenology, 2016, 第 1 作者
(19) The dnd RNA identifies germ cell origin and migration in Olive flounder (Paralichthysolivaceus)., BioMed Research. International, 2016, 通讯作者
(20) Effects of chilling storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod (Gadus macrocephalus). Chinese Journal of Oceanology and Limnology, Chinese Journal of Oceanology and Limnology, 2016, 通讯作者
(21) Summer flounder (Paralichthysdentatus) sperm cryopreservation and application in interspecific hybridization with oliver flounder (P. olivaceus)., Theriogenology, 2015, 第 1 作者
(22) The dnd RNA identifies germ cell origin and migration in Olive flounder (Paralichthys olivaceus). , BioMed Research Internationa, 2015, 第 1 作者
(23) Effects of chilling storage and cryopreservation on sperm characteristics, antioxidant enzyme activity, and lipid peroxidation in Pacific cod (Gadus macrocephalus). , Chinese Journal of Oceanology and Limnology., 2015, 通讯作者
(24) Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with oliver flounder (P. olivaceus), Theriogenology, 2015, 第 1 作者
(25) Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation , Fish Physiol Biochem, 2015, 第 1 作者
(26) 太平洋牡蛎(Crassostreagigas)精液的超低温保存研究, 海洋与湖沼, 2015, 通讯作者
(27) 太平洋鳕(Gadusmacrocephalus)精液超低温冷冻方法的建立及精子超微结构分析, 海洋与湖沼, 2015, 通讯作者
(28) 大菱鲆胃质子泵(H+ /K+ ATPase)与消化机能发生的关系, 海 洋 与 湖 沼, 2015, 通讯作者
(29) 条纹锯鮨精液超低温冷冻保存研究, 水产学报, 2015, 通讯作者
(30) Histological and enzymatic responses of Japanese flounder (Paralichthys olivaceus) and its hybrids (P. olivaceus♀×P. dentatus♂) to chronic heat stress, Fish Physiology and Biochemistry, 2014, 通讯作者
(31) Histological change and heat shock protein 70 expression in different tissues of Japanese flounder (Paralichthys olivaceus) to elevated temperature, Chinese Journal of Oceanology and Limnology, 2014, 通讯作者
(32) The viability,melanophore and embryo genesis of first- and second- generation hybrids between Paralichthys olivaceus and P.dentatus, Marine Biology Reserch, 2013, 通讯作者
(33) Germline-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus), Theriogenology, 2013, 通讯作者
(34) Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development, Comparative Biochemistry and Physiology, Part B, 2013, 通讯作者
(35) Marine Biology Reserch, 2013, 通讯作者
(36) The viability, melanophore and embryo genesis of first and second generation hybrids between Paralichthys olivaceus and P. dentatus., Marine Biology Research, 2012, 第 1 作者
(37) Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream, Int. J. Biol. Sci., 2012, 第 1 作者
(38) Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations, Journal of Fish Biology, 2012, 第 3 作者
(39) Study on permeability of dmso in embryos of red seabream (pagrus major) by capillary electrophoresis, Cryoletters, 2011, 第 1 作者
(40) Stages of Rock Bream Oplegnathus fasciatus (Temminck et Schlegel 1844): Embryonic Development, Aquaculture research, 2011, 第 3 作者
(41) Effect of storage time and cryoprotectant concentration on the quality of cryopreserved sperm in red seabream (Pagrus major), Aquaculture research, 2010, 第 1 作者
(42) Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm, Cryobiology, 2010, 第 1 作者
(43) Changes in RNA, DNA, protein contents and growth of turbot Scophthalmus maximus larvae and juveniles, Journal of Fish Biology , 2010, 第 2 作者
(44) Ontogeny of Gastrointestinal Tract in Hybrid Flounder Jasum, Paralichthys olivaceus × P. dentatus, Journal of the World Aquaculture Society, 2010, 第 3 作者
(45) 长期超低温保存后真鲷精子的质量变化, 海洋科学, 2010, 第 2 作者
(46) 条斑星鲽幼鱼变态期间核酸及蛋白的变化, 海洋科学, 2010, 第 3 作者
(47) 条石鲷鳔器官早期发育的组织学观察, 海洋科学, 2010, 第 3 作者
(48) Flow cytometry and ultrastructure of cryopreserved red seabream (Pagrus major) sperm, Theriogenology, 2007, 第 1 作者
(49) Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major), Aquaculture, 2007, 第 1 作者
(50) An Efficient Methodology for Cryopreservation of permatozoa of Red Seabream, Pagrus major, with 2-mL Cryovials, Journal of the world aquaculture society, 2006, 第 1 作者
(51) 运用计算机辅助分析检测超低温保存的真鲷(Pagrosomus major)精子的质量, 自然科学进展, 2006, 第 1 作者