许琳 - 南京工业大学 - 生物与制药工程学院

个人简介

许琳，教授，博士生导师。中国免疫学会会员，美国化学学会会员。1983年于白求恩医科大学（现吉林大学医学部）获免疫学硕士学位，1988-1995年任第二军医大学南京军医学院讲师，1992年于中山大学获微生物与免疫学博士学位，1995-2003年任第二军医大学海军军医学院教授，1997-1998年香港理工大学应用生物学及化学技术系交流学者，2002-2003年美国亚利桑那大学神经生物学研究所高级访问学者，2004年至今在南京工业大学从事分子生物学与基因工程的科研和教学工作，作为基因工程研究室负责人，参与了包括国家973项目、国家自然科学基金等在内的多项国家与省部级项目。在国内外检索期刊上发表论文60余篇，其中SCI论文20余篇，申请国家发明专利10余项。《中国国防报》曾以“从女战士到女博士——我国首位利用人体基因抗癌的科学家”为题报道了许琳教授在我国基因工程领域的突出成果。所负责的基因工程与生物信息学研究室已培养博士生8人，硕士生30余人。课题组致力于发展和应用工业微生物的现代研究方法，以复杂生物网络研究为基础，依托实验室在基因工程与生物信息学领域的积累，以现代生物技术设计和创造符合工业化需求的微生物，服务于传统生物产业技术的提升

研究领域

1、酶的基因工程与蛋白质工程筛选重要生物资源与酶蛋白，进行重要酶分子体外/体内分子改造，优化其分子特性；发展新的表达体系，显著提高酶的异源表达水平，开发并提高医药用酶、食品用酶、环境用酶等的规模化制备水平。 2、系统生物学与代谢工程利用系统生物学的技术与方法，研究工业微生物生命活动的物质基础与过程机制，进行工业微生物细胞的系统生物技术改造，优化微生物细胞特定代谢途径和代谢网络，提高发酵产品的产率与效率。 3、生物催化与生物医药技术开展工业生物催化剂及其催化过程的研究，采用酶、酶系或全细胞催化技术，生产化学法难以转化与合成的新型生物产品。开发化工原料、医药中间体等高值精细化学品的高效生物合成技术

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

1. Yang X, Lei G, Ying H, Xu L, Yan M: Enhancing the production of uridine 5'-monophosphate by recombinant Saccharomyces cerevisiae using a whole cell biocatalytic process. World Journal of Microbiology and Biotechnology 2011:1-7. 2. Liu H, Liu K, Yan M, Xu L, Ouyang P: gTME for Improved Adaptation of Saccharomyces cerevisiae to Corn Cob Acid Hydrolysate. Applied Biochemistry and Biotechnology 2011:1-10. 3. Ye Q, Li X, Yan M, Cao H, Xu L, Zhang Y, Chen Y, Xiong J, Ouyang P, Ying H: High-level production of heterologous proteins using untreated cane molasses and corn steep liquor in Escherichia coli medium. Applied microbiology and biotechnology 2010, 87(2):517-525. 4. Ye Q, Cao H, Zang G, Mi L, Yan M, Wang Y, Zhang Y, Li X, Li J, Xu L: Biocatalytic synthesis of (S)-4-chloro-3-hydroxybutanoate ethyl ester using a recombinant whole-cell catalyst. Applied microbiology and biotechnology 2010:1-9. 5. Ye Q, Cao H, Yan M, Cao F, Zhang Y, Li X, Xu L, Chen Y, Xiong J, Ouyang P: Construction and co-expression of a polycistronic plasmid encoding carbonyl reductase and glucose dehydrogenase for production of ethyl (S)-4-chloro-3-hydroxybutanoate. Bioresource technology 2010, 101(17):6761-6767. 6. Ye Q, Cao H, Mi L, Yan M, Wang Y, He Q, Li J, Xu L, Chen Y, Xiong J: Biosynthesis of (S)-4-chloro-3-hydroxybutanoate ethyl using Escherichia coli co-expressing a novel NADH-dependent carbonyl reductase and a glucose dehydrogenase. Bioresource technology 2010. 7. Yao T, Xu L, Ying H, Huang H, Yan M: The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate. Applied Biochemistry and Biotechnology 2010, 160(3):694-703. 8.Yang X, Xu L, Yan M: Modeling metabolic response to changes of enzyme amount in yeast glycolysis. African Journal of Biotechnology 2010, 9(41):6894-6901. 9. Yang C, Xu L, Yan L, Xu Y: Construction of a genetically engineered microorganism with high tolerance to arsenite and strong arsenite oxidative ability. Journal of Environmental Science and Health Part A 2010, 45(6):740-745. 10. Liu H, Yan M, Lai C, Xu L, Ouyang P: gTME for improved xylose fermentation of Saccharomyces cerevisiae. Applied Biochemistry and Biotechnology 2010, 160(2):574-582. 11. Cao H, Mi L, Ye Q, Zang G, Yan M, Wang Y, Zhang Y, Li X, Xu L, Xiong J et al: Purification and characterization of a novel NADH-dependent carbonyl reductase from Pichia stipitis involved in biosynthesis of optically pure ethyl (S)-4-chloro-3-hydroxybutanoate. Bioresource technology 2010. 12. Ye Q, Yan M, Yao Z, Xu L, Cao H, Li Z, Chen Y, Li S, Bai J, Xiong J: A new member of the short-chain dehydrogenases/reductases superfamily: Purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis. Bioresource technology 2009, 100(23):6022-6027. 13. Ye Q, Yan M, Xu L, Cao H, Li Z, Chen Y, Li S, Ying H: A novel carbonyl reductase from Pichia stipitis for the production of ethyl (S)-4-chloro-3-hydroxybutanoate. Biotechnology letters 2009, 31(4):537-542. 14.Xu W, Yan M, Xu L, Ding L, Ouyang P: Engineering the activity of thermophilic xylose isomerase by site-directed mutation at subunit interfaces. Enzyme and Microbial Technology 2009, 44(2):77-83. 15.Xu W, Cai P, Yan M, Xu L, Ouyang P: Molecular Dynamics Simulation of Temperature-dependent Flexibility of Thermophilic Xylose Isomerase. Chinese Journal of Chemical Physics 2009, 22:467. 16. Wang K, Zheng C, Wu C, Gao M, Liu Q, Yang K, Ellsworth K, Xu L, Wu J: [alpha]-Chloralose diminishes [gamma] oscillations in rat hippocampal slices. Neuroscience letters 2008, 441(1):66-71. 17. XU W, CAI P, YAN M, XU L, OUYANG PK: Molecular docking of xylitol and xylose isomerase from Thermus thermophilus and model analysis. Chem J Chin Univ 2007, 28:971-973. 18. Li Y, Yan M, Wang K, Xu L, Xu W, Ouyang P: Study on dsRNA Mediated Gene Silencing in Saccharomyces cerevisiae: Suppressing the Expression of GRE3 Gene. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 2007, 34(3):292-298.