Leong, Kam W. - Columbia University - Department of Biomedical Engineering

个人简介

1975 – 1977 University of California, Santa Barbara, CA B.S. in Chemical Engineering 1977 – 1982 University of Pennsylvania, Philadelphia, PA Ph.D. in Chemical Engineering (Degree conferred 1987) 1982 – 1986 Massachusetts Institute of Technology, Cambridge, MA Research Associate in Whitaker College of Health Sciences and Technology and Department of Applied Biological Science PhD Dissertation: Synthesis of Graphite Oxide Intercalation Compounds PhD Advisor: William C Forsman

研究领域

1) Biomaterials strategies to modulate inflammation: Many chronic disabling diseases such as rheumatoid arthritis, psoriasis, multiple sclerosis and systemic lupus erythematosus are increasingly linked to inappropriate and chronic activation of inflammatory cells. A central event in the pathogenesis of these diseases appears to be an aberrant activation of innate immune sensors, most prominently the Pattern Recognition Receptors (PRRs), by nucleic acids that are released from dead and dying cells. We design nucleic acid-binding polymers in the configuration of either soluble or immobilized polycation to scavenge these PRRs as a molecular strategy to combat inflammation. 2) Microphysiological system for disease modeling and drug development: Inadequate animal models and poor access to living human brain lead to significant challenges to model psychiatric diseases and to develop neuropsychiatric drugs. Recent advances in human induced pluripotent stem cells (hiPSC) have made it possible to create a patient-specific brain-like neural tissue (referred to as ‘cerebral organoid’) that displays an architecture and neural network activity resembling that of human brain. We generate cerebral organoids from patients with genetic lesions to investigate disease mechanisms responsible for the development of neuropsychiatric disorders in humans. We also generate tissue-engineered blood vessels from induced smooth muscle cells to model Marfan Syndrome and atherosclerosis. 3) Nonviral gene editing: Genome editing offers promising solutions to genetic disorders by editing DNA sequences or modulating gene expression. Efficient in vivo delivery remains a significant challenge to the realization of the clinical potential of gene editing. We design nanocarriers with targeting characteristics to achieve efficient gene editing in various tissues. We are evaluating the in vivo efficacy of such a gene editing system that targets three separate genes correlated with cardio-protective point mutations.

近期论文

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Lin PY, Chiu YL, Huang JH, Chuang EY, Mi FL, Lin KJ, Juang JH, Sung HW, and Leong KW. https://doi.org/10.1002/advs.201701079 Nonviral Oral Gene Delivery for Chronic Protein Replacement Therapy. Advanced Science. Lao YH, Li MQ, Gao MA, Ho TC, Jiang W, and Leong KW. https://doi.org/10.1002/advs.201700540 HPV oncogene manipulation using non-virally delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute. Advanced Science. Chen ZZ, Tang M, Huang D, Jiang W, Li M, Ji H, Park JS, Xu B, Atchison L, Truskey G, and Leong KW. (In Press) Real-time observation of leukocyte-endothelium interactions in tissue-engineered blood vessel. Labon-Chip. Zeng Y, Wong ST, Teo SK, Leong KW, Chiam KH, and Yim EKF. (2018) Human mesenchymal stem cell basal membrane bending on gratings is dependent on both grating width and curvature. Sci Rep, 8(1): 6444. Xue Q, Liu XB, Lao YH, Wu LP, Wang D, Zuo ZQ, Chen JY, Hou J, Bei YY, Wu XF, Leong KW, Xiang H,and Han J. (2018) Anti-infective biomaterials with surface-decorated tachyplesin I. Biomaterials. Wang HX, Song Z, Lao YH, Xu X, Gong J, Cheng D, Chakraborty S, Park JS, Li M, Huang D, Yin L, Cheng J,and Leong KW. (2018) Nonviral gene editing via CRISPR/Cas9 delivery by membrane-disruptive and endosomolytic helical polypeptide. Proc Natl Acad Sci U S A, 115(19): 4903-4908. Suryaprakash S, Li MQ, Lao YH, Wang HX, and Leong KW. (2018) Graphene oxide cellular patches for mesenchymal stem cell-based cancer therapy. Carbon, 129: 863-868. Shao D, Li M, Wang Z, Zheng X, Lao YH, Chang Z, Zhang F, Lu M, Yue J, Hu H, Yan H, Chen L, Dong WF,and Leong KW. (2018) Bioinspired Diselenide-Bridged Mesoporous Silica Nanoparticles for DualResponsive Protein Delivery. Adv Mater: e1801198. Qiao D, Liu L, Chen Y, Xue C, Gao Q, Mao HQ, Leong KW, and Chen Y. (2018) Potency of a Scalable Nanoparticulate Subunit Vaccine. Nano Lett, 18(5): 3007-3016. Pinese C, Lin J, Milbreta U, Li M, Wang Y, Leong KW, and Chew SY. (2018) Sustained delivery of siRNA/mesoporous silica nanoparticle complexes from nanofiber scaffolds for long-term gene silencing.Acta Biomater. Meng H, Leong W, Leong KW, Chen C, and Zhao Y. (2018) Walking the line: The fate of nanomaterials at biological barriers. Biomaterials, 174: 41-53. Kim HS, Son YJ, Mao W, Leong KW, and Yoo HS. (2018) Atom Transfer Radical Polymerization of Multishelled Cationic Corona for the Systemic Delivery of siRNA. Nano Lett, 18(1): 314-325. He Z, Liu Z, Tian H, Hu Y, Liu L, Leong KW, Mao HQ, and Chen Y. (2018) Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.Nanoscale, 10(7): 3307-3319. Chan HF, Ma S, Tian J, and Leong KW. (2017) High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets. Nanoscale, 9(10): 3485-3495. Charoensook SN, Williams DJ, Chakraborty S, Leong KW, and Vunjak-Novakovic G. (2017) Bioreactor model of neuromuscular junction with electrical stimulation for pharmacological potency testing. Integr Biol (Camb), 9(12): 956-967. Christoforou N, Chakraborty S, Kirkton RD, Adler AF, Addis RC, and Leong KW. (2017) Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage. Sci Rep, 7: 40285. He Z, Santos JL, Tian H, Huang H, Hu Y, Liu L, Leong KW, Chen Y, and Mao HQ. (2017) Scalable fabrication of size-controlled chitosan nanoparticles for oral delivery of insulin. Biomaterials, 130: 28-41. Ji H, Kim HS, Kim HW, and Leong KW. (2017) Application of induced pluripotent stem cells to model smooth muscle cell function in vascular diseases. Curr Opin Biomed Eng, 1: 38-44. Kim HS, Son YJ, Mao W, Leong KW, and Yoo HS. (2018) Atom Transfer Radical Polymerization of Multishelled Cationic Corona for the Systemic Delivery of siRNA. Nano Lett, 18(1): 314-325. Kim JW, Mahapatra C, Hong JY, Kim MS, Leong KW, Kim HW, and Hyun JK. (2017) Functional Recovery of Contused Spinal Cord in Rat with the Injection of Optimal-Dosed Cerium Oxide Nanoparticles. Adv Sci (Weinh), 4(10): 1700034. Lee J, Jackman JG, Kwun J, Manook M, Moreno A, Elster EA, Kirk AD, Leong KW, and Sullenger BA.(2017) Nucleic acid scavenging microfiber mesh inhibits trauma-induced inflammation and thrombosis.Biomaterials, 120: 94-102. Li M, Jiang W, Chen Z, Suryaprakash S, Lv S, Tang Z, Chen X, and Leong KW. (2017) A versatile platform for surface modification of microfluidic droplets. Lab Chip, 17(4): 635-639.