Wurm, Florian - École polytechnique fédérale de Lausanne EPFL - Institute of Chemical Sciences and Engineering

个人简介

Florian Wurm received his academic training as a biologist and molecular geneticist at the University of Giessen, Germany. He joined the subsidiary of Hoechst AG, Behringwerke in Marburg as head of a laboratory in Virology. Working with immortalized mammalian cells for the establishment of production processes for alpha-interferons provided the first opportunity to combine basic research with medical application. In 1984 he joined Harvard Medical School in Boston as a Research Fellow in Molecular Biology. 1986 he took an offer from Genentech Inc. in San Francisco to work in Process Sciences on the (Upstream) Development of large scale manufacturing processes for recombinant proteins. He has held a number of leading positions at Genentech and has acquired intimate knowledge on the creation of novel pharmaceutical components based on recombinant DNA technology. In 1995 he joined the EPFL as a newly appointed Professor for Biotechnology. F.M. Wurm has published more than 200 scientific papers and holds 20 patents. He is member of the Executive Board of the European Society of Animal Cell Technology and he is or was a member of the Scientific Advisory Boards of a number of companies as well as of the International Aids Vaccine Initiative, an institution of the Rockefeller Foundation. Prof. Wurm serves as a consultant to the pharmaceutical biotechnology industry, mainly in the fields of animal cell technology for recombinant protein production and in regulatory affairs. He works in an editorial role and as scientific reviewer for a number of international journals, such as Nature Biotechnology, Journal of Biotechnology, Biotechnology and Bioengineering, Biotechnology Progress and Cytotechnology. F.M. Wurm teaches classes to pre- and postgraduate students in the fields of Molecular and Pharmaceutical Biotechnology at EPFL. He is founder and Chief Scientific Officer of ExcellGene SA, a company established in 2001 in Monthey, Switzerland. In 2008 Dr. Wurm was appointed Visiting Professor for Biotechnology at Jinan University in Guangzhou, China. Academic Education 1976: Justus Liebig University of Giessen, Germany: Diploma in Biology (summa cum laude) 1979: Justus Liebig University of Giessen, Germany, Institut für Genetik, Prof. Dr. Fritz Anders: Dr. rer. nat. (cum laude) Professional activities 1980 to 1985: Behringwerke Marburg Hoechst AG, Dept. of Virology, Scientist/Groupleader 1985 to 1986: Harvard Medical School, Boston, Massachusetts, USA (Massachusetts Gen. Hospital, Dept. of Mol. Biology): Visiting Scientist 1986 to 1995: Genentech Inc., South San Francisco, California, USA (Cell Culture Research & Development, Process Sciences): Scientist, Group-Leader, Senior Scientist, Project-Team-Leader, Senior Manager 1995: Swiss Federal Institute of Technology, Lausanne, Switzerland: Associate Professor 1995 - 1998: Laboratory of Cellular Biotechnology, Centre de Biotechnologie UNIL-EPFL, ISP-FSB, Associate Professor of Biotechnology Co-Director Centre de Biotechnologie UNIL-EPFL 1996 - 1999: Coordinator Biotechnology Priority Program (SPP) of the Swiss National Science Foundation (SNF) April 1998 - present: Full Professor of Biotechnolgy, Faculty of Life Science, EPFL

研究领域

Several research projects addressing gene transfer, genetics, cytogenetics of recombinant protein expression in mammalian cells Remark: Several of the projects for which I had full or partial managerial and scientific responsibility are now marketed products, such as Activase®-TPA, Herceptin®-anti Her2antibody, TNKase-TPA, Pulmozyme®-huDNase. Process development work was done for cell cultures up to the 10 000 Liter scale.

近期论文

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F. Davami, L. Baldi, Y. Rajendra and F. M Wurm. Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells, in International journal of molecular and cellular medicine, vol. 3, num. 3, p. 146-56, 2014. M. Reclari, M. Dreyer, S. Tissot, D. Obreschkow and F. M. Wurm et al. Surface wave dynamics in orbital shaken cylindrical containers, in Physics of Fluids, vol. 26, num. 5, p. 052104, 2014. T. Memanishvili, N. Zavradashvili, N. Kupatadze, D. Tugushi and M. Gverdtsiteli et al. Arginine-Based Biodegradable Ether–Ester Polymers with Low Cytotoxicity as Potential Gene Carriers, in Biomacromolecules, p. 140711090449007, 2014. X. Shen, D. L. Hacker, L. Baldi and F. M. Wurm. Virus-free transient protein production in Sf9 cells, in Journal Of Biotechnology, vol. 171, p. 61-70, 2014. H. Kilpinen, S. M. Waszak, A. R. Gschwind, S. K. Raghav and R. M. Witwicki et al. Coordinated Effects of Sequence Variation on DNA Binding, Chromatin Structure, and Transcription, in Science, vol. 342, num. 6159, p. 744-747, 2013. F. Zagari, M. Stettler, L. Baldi, H. Broly and F. M. Wurm et al. High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture, in Pharmaceutical Bioprocessing, vol. 1, num. 1, p. 19-27, 2013. D. T. Monteil, G. Tontodonati, S. Ghimire, L. Baldi and D. L. Hacker et al. Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension, in Biochemical Engineering Journal, 2013. M. Discacciati, D. Hacker, A. Quarteroni, S. Quinodoz and S. Tissot et al. Numerical simulation of orbitally shaken viscous fluids with free surface, in International Journal For Numerical Methods In Fluids, vol. 71, num. 3, p. 294-315, 2013. S. Tissot, P. Michel, C. Douet, S. Grezet and L. Baldi et al. Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures, in Processes, vol. 1, num. 1, p. 3-11, 2013. Z. Kadlecova, Y. Rajendra, M. Matasci, L. Baldi and D. L. Hacker et al. DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine, in Journal of Controlled Release, 2013. J.-R. Alattia, M. Matasci, M. Dimitrov, L. Aeschbach and S. Balasubramanian et al. Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach., in Biotechnol Bioeng., 2013. A. Angelini, P. Diderich, J. Morales-Sanfrutos, S. Thurnheer and D. Hacker et al. Chemical Macrocyclization of Peptides Fused to Antibody Fc Fragments, in Bioconjugate Chemistry, vol. 23, num. 9, p. 1856-1863, 2012. Z. Kadlecova, L. Baldi, D. Hacker, F. M. Wurm and H.-A. Klok. Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues, in Biomacromolecules, vol. 13, num. 10, p. 3127-3137, 2012. P. O. Michel, C. Degen, M. Hubert, L. Baldi and D. L. Hacker et al. A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells, in Analytical Biochemistry, vol. 430, num. 2, p. 138-140, 2012. A. Angelini, P. Diderich, J. Morales-Sanfrutos, S. Thurnheer and D. Hacker et al. Chemical macrocyclization of peptides fused to antibody Fc fragments, in Bioconjugate chemistry, vol. 23, num. 9, p. 1856-63, 2012. W. Klöckner, S. Tissot, F. Wurm and J. Büchs. Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors, in Biochemical Engineering Journal, vol. 65, p. 63-69, 2012. F. Zagari, M. Jordan, M. Stettler, H. Broly and F. M. Wurm. Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity, in New Biotechnology, 2012. Z. Kadlecova, Y. Rajendra, M. Matasci, D. Hacker and L. Baldi et al. Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells, in Macromolecular Bioscience, vol. 12, p. 794-804, 2012. Y. Rajendra, D. Kiseljak, S. Manoli, L. Baldi and D. L. Hacker et al. Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells, in Biotechnology and Bioengineering, p. n/a-n/a, 2012. Z. Kadlecova, S. Nallet, D. L. Hacker, L. Baldi and H.-A. Klok et al. Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups, in Macromolecular Bioscience, vol. 12, p. 628-636, 2012.