个人简介
1992-1996 华东理工大学,生物化工专业,工学学士
1996-1999 江南大学,应用化学系,助教
1999-2002 华东理工大学,生物化工专业,工学硕士
2002-2006 美国弗吉尼亚联邦大学,化学与生命科学专业,工学博士
2006-2010 江南大学,生物工程学院,副教授
2010-至今 江南大学,生物工程学院,教授
研究领域
长期从事手性生物催化和酶工程的研究,主要承担分子生物学基础、生物催化工艺学课程的讲授工作
近期论文
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Structural Insight into Enantioselective Inversion of an Alcohol Dehydrogenase Reveals a “Polar Gate” in Stereorecognition of Diaryl Ketones. Journal of the American Chemical Society (2018) 140: 12645-12654
Hydroclassified Combinatorial Saturation Mutagenesis: Reshaping Substrate Binding Pockets of KpADH for Enantioselective Reduction of Bulky-bulky Ketones. ACS Catalysis (2018) 8: 8336–8345
Engineering an Alcohol Dehydrogenase for Balancing Kinetics in NADPH Regeneration with 1,4-Butanediol as a Cosubstrate. ACS Sustainable Chemistry & Engineering (2019) 7: 15706−15714
Hyperconjugation promoted by hydrogen bonding between His98/His241 and a carboxyl group contributes to tyrosine decarboxylase catalysis. Catalysis Science & Technology (2019) 9: 6222-6226
Engineering of Cyclodextrin Glycosyltransferase Reveals pH-Regulated Mechanism of Enhanced Long-Chain Glycosylated Sophoricoside Specificity. Applied and Environmental Microbiology (2020) 86: e00004-20
Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB. Biotechnology for Biofuels (2019) 12:164
Novel dihydrogen-bonding deep eutectic solvents: pretreatment of rice straw for butanol fermentation featuring enzyme recycling and high solvent yield. Chemical Engineering Journal (2018) 333:712-720
Carbonyl group-dependent high-throughput screening and enzymatic characterization of diaromatic ketone reductase. Catalysis Science & Technology (2016) 6: 6320-6327
DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli. Biotechnol Biofuels (2016) 9: 114