Huang, Wei - University of Oxford - Department of Engineering Science

个人简介

Activities Associate Editor for Environmental Microbiology Associate Editor for Microbial Biotechnology EPSRC Fellowship on Synthetic Biology Developing SimCells (simple and simulated cells) for synthetic biology One of pioneers studying microbes at single cell level using Raman micro-spectroscopy Pioneer employing ultrasound to transfer DNA to bacterial cells Current projects EPSRC, Fellowship for synthetic biology, PI, Development of SimCells as building blocks for synthetic biology. 2014-2019 EPSRC, Flashlight Synthetic Biology fund, shared PI, Engineering a semi-biotic immune system. 2010-2015 NERC, Co-I, Biogeochemical cycling of N-osmolytes in the surface ocean (PI: Dr Yin Chen, Warwick University). 2014-2017 BBSRC sLoLa, Co-I, Engineering new capacities for solar energy utilisation in bacteria (PI: Prof Neil Hunter, University of Sheffield). 2015-2020 EPSRC, Co-I, Liquid Fuel and bioEnergy Supply from CO2 Reduction (PI: Prof Keith Scott, Newcastle University). 2016-2020 Past projects EU grant ECOWATER, USFD PI, Shared with Corus Ltd and CSM. 2010-2013 Royal Society Research fund 2009/R2, PI, Biosensor cell arrays for monitoring environmental pollution. 2010 NERC, Innovative A, PI, Novel biosensor for genotoxicity detection.2010 EPSRC, KTA fund, PI, Development of bacterial biosensors. 2009 NERC grant, PI, Interaction of nanoparticles with microbial populations during particle transport. 2007 NERC, Innovative Grant, New Concept of Biosensors. 2005-2006 EC FP7, Co-I, Soil transformations in European catchments (PI: Prof Steve Banwart, University of Sheffield). 2009-2014 EPSRC, Co-I, BioEngineering from first principles (PI: Prof Steve Banwart, University of Sheffield). 2010

研究领域

1) Synthetic biology (SimCells, biosensors and bioenergy) 2) Single cell Raman biotechnology (Raman activated cell sorting for single cell -omics).

近期论文

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Synthetic Biology Lim B, Yin Y, Ye H, Cui Z, Papachristodoulou A, Huang WE. Reprogramming Synthetic Cells for Targeted Cancer Therapy. ACS Synth Biol. 2022 Mar 18;11(3):1349-1360. doi: 10.1021/acssynbio.1c00631. Chen JX, Lim B, Steel H, Song Y, Ji M, Huang WE. Redesign of ultrasensitive and robust RecA gene circuit to sense DNA damage. Microb Biotechnol. 2021 Nov;14(6):2481-2496. doi: 10.1111/1751-7915.13767. Ng CK, Putra SL, Kennerley J, Habgood R, Roy RA, Raymond JL, Thompson IP, Huang WE. Genetic engineering biofilms in situ using ultrasound-mediated DNA delivery. Microb Biotechnol. 2021 Jul;14(4):1580-1593. doi: 10.1111/1751-7915.13823. Lim B, Ratcliff J, Nawrot DA, Yu Y, Sanghani HR, Hsu CC, Peto L, Evans S, Hodgson SH, Skeva A, Adam M, Panopoulou M, Zois CE, Poncin K, Vasudevan SR, Dai S, Ren S, Chang H, Cui Z, Simmonds P, Huang WE, Andersson MI. Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection. Sci Rep. 2021 Aug 10;11(1):16193. doi: 10.1038/s41598-021-95607-1. Zeng H, Rohani R, Huang WE, Yang A. Understanding and mathematical modelling of cellular resource allocation in microorganisms: a comparative synthesis. BMC Bioinformatics. 2021 Sep 28;22(1):467. doi: 10.1186/s12859-021-04382-3. Chen XJ, Wang B, Thompson IP, Huang WE. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells. ACS Synth Biol. 2021 Oct 15;10(10):2566-2578. doi: 10.1021/acssynbio.1c00223. Chen JX, Lim B, Steel H, Song Y, Ji M, Huang WE. Redesign of ultrasensitive and robust RecA gene circuit to sense DNA damage. Microb Biotechnol. 2021 Nov;14(6):2481-2496. doi: 10.1111/1751-7915.13767. Martin LK, Huang WE, Thompson IP. Bacterial wax synthesis. Biotechnol Adv. 2021 Jan-Feb;46:107680. doi: 10.1016/j.biotechadv.2020.107680. Soldan R, Sanguankiattichai N, Bach-Pages M, Bervoets I, Huang WE, Preston GM. From macro to micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization. Environ Microbiol. 2021 Apr;23(4):2070-2085. doi: 10.1111/1462-2920.15296. Huang WE, Lim B, Hsu CC, Xiong D, Wu W, Yu Y, Jia H, Wang Y, Zeng Y, Ji M, Chang H, Zhang X, Wang H, Cui Z. RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2. Microb Biotechnol. 2020 Jul;13(4):950-961. doi: 10.1111/1751-7915.13586. Cui Z, Chang H, Wang H, Lim B, Hsu CC, Yu Y, Jia H, Wang Y, Zeng Y, Ji M, Liu W, Inverarity C, Huang WE. Development of a rapid test kit for SARS-CoV-2: an example of product design. Biodes Manuf. 2020 May 11;:1-4. doi: 10.1007/s42242-020-00075-7. Fan C, Davison PA, Habgood R, Zeng H, Decker CM, Gesell Salazar M, Lueangwattanapong K, Townley HE, Yang A, Thompson IP, Ye H, Cui Z, Schmidt F, Hunter CN, Huang WE. Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology. Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6752-6761. doi: 10.1073/pnas.1918859117. Song Y, Cartron ML, Jackson PJ, Davison PA, Dickman MJ, Zhu D, Huang WE, Hunter CN. Proteorhodopsin Overproduction Enhances the Long-Term Viability of Escherichia coli. Appl Environ Microbiol. 2019 Dec 13;86(1). doi: 10.1128/AEM.02087-19. Fan C, Wu YH, Decker CM, Rohani R, Gesell Salazar M, Ye H, Cui Z, Schmidt F, Huang WE. Defensive Function of Transposable Elements in Bacteria. ACS Synth Biol. 2019 Sep 20;8(9):2141-2151. doi: 10.1021/acssynbio.9b00218. Ng CK, Putra SL, Kennerley J, Habgood R, Roy RA, Raymond JL, Thompson IP, Huang WE. Genetic engineering biofilms in situ using ultrasound-mediated DNA delivery. Microb Biotechnol. 2021 Jul;14(4):1580-1593. doi: 10.1111/1751-7915.13823. Timmis K, Cavicchioli R, Garcia JL, Nogales B, Chavarría M, Stein L, McGenity TJ, Webster N, Singh BK, Handelsman J, de Lorenzo V, Pruzzo C, Timmis J, Martín JLR, Verstraete W, Jetten M, Danchin A, Huang W, Gilbert J, Lal R, Santos H, Lee SY, Sessitsch A, Bonfante P, Gram L, Lin RTP, Ron E, Karahan ZC, van der Meer JR, Artunkal S, Jahn D, Harper L. The urgent need for microbiology literacy in society. Environ Microbiol. 2019 May;21(5):1513-1528. doi: 10.1111/1462-2920.14611. Huang WE, Nikel PI. The Synthetic Microbiology Caucus: from abstract ideas to turning microbes into cellular machines and back. Microb Biotechnol. 2019 Jan;12(1):5-7. doi: 10.1111/1751-7915.13337. Zhang Q, Song Y, Amor K, Huang WE, Porcelli D, Thompson I. Monitoring Cr toxicity and remediation processes - combining a whole-cell bioreporter and Cr isotope techniques. Water Res. 2019 Apr 15;153:295-303. doi: 10.1016/j.watres.2019.01.009. Chen JX, Steel H, Wu YH, Wang Y, Xu J, Rampley CPN, Thompson IP, Papachristodoulou A, Huang WE. Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells. Appl Environ Microbiol. 2019 Mar 15;85(6). doi: 10.1128/AEM.02959-18. Rampley CPN, Davison PA, Qian P, Preston GM, Hunter CN, Thompson IP, Wu LJ, Huang WE. Development of SimCells as a novel chassis for functional biosensors. Sci Rep. 2017 Aug 3;7(1):7261. doi: 10.1038/s41598-017-07391-6. Hedbavna P, Rolfe SA, Huang WE, Thornton SF. Biodegradation of phenolic compounds and their metabolites in contaminated groundwater using microbial fuel cells. Bioresour Technol. 2016 Jan;200:426-34. doi: 10.1016/j.biortech.2015.09.092. Zhang D, Ding A, Cui S, Hu C, Thornton SF, Dou J, Sun Y, Huang WE. Whole cell bioreporter application for rapid detection and evaluation of crude oil spill in seawater caused by Dalian oil tank explosion. Water Res. 2013 Mar 1;47(3):1191-200. doi: 10.1016/j.watres.2012.11.038. Zhang D, Zhao Y, He Y, Wang Y, Zhao Y, Zheng Y, Wei X, Zhang L, Li Y, Jin T, Wu L, Wang H, Davison PA, Xu J, Huang WE. Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1. ACS Synth Biol. 2012 Jul 20;1(7):274-83. doi: 10.1021/sb3000244. Zhang D, He Y, Wang Y, Wang H, Wu L, Aries E, Huang WE. Whole-cell bacterial bioreporter for actively searching and sensing of alkanes and oil spills. Microb Biotechnol. 2012 Jan;5(1):87-97. doi: 10.1111/j.1751-7915.2011.00301.x. Wang Y, Chen Y, Zhou Q, Huang S, Ning K, Xu J, Kalin RM, Rolfe S, Huang WE. A culture-independent approach to unravel uncultured bacteria and functional genes in a complex microbial community. PLoS One. 2012;7(10):e47530. doi: 10.1371/journal.pone.0047530. Zhang D, Fakhrullin RF, Özmen M, Wang H, Wang J, Paunov VN, Li G, Huang WE. Functionalization of whole-cell bacterial reporters with magnetic nanoparticle. Microb Biotechnol. 2011 Jan;4(1):89-97. doi: 10.1111/j.1751-7915.2010.00228.x. Rico A, Bennett MH, Forcat S, Huang WE, Preston GM. Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum. PLoS One. 2010 Jan 29;5(1):e8977. doi: 10.1371/journal.pone.0008977. Song Y, Li G, Thornton SF, Thompson IP, Banwart SA, Lerner DN, Huang WE. Optimization of bacterial whole cell bioreporters for toxicity assay of environmental samples. Environ Sci Technol. 2009 Oct 15;43(20):7931-8. doi: 10.1021/es901349r. Huang WE, Singer AC, Spiers AJ, Preston GM, Whiteley AS. Characterizing the regulation of the Pu promoter in Acinetobacter baylyi ADP1. Environ Microbiol. 2008 Jul;10(7):1668-80. doi: 10.1111/j.1462-2920.2008.01583.x. Song Y, Hahn T, Thompson IP, Mason TJ, Preston GM, Li G, Paniwnyk L, Huang WE. Ultrasound-mediated DNA transfer for bacteria. Nucleic Acids Res. 2007;35(19):e129. doi: 10.1093/nar/gkm710. Huang WE, Huang L, Preston GM, Naylor M, Carr JP, Li Y, Singer AC, Whiteley AS, Wang H. Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified biosensor strain of Acinetobacter sp. ADP1. Plant J. 2006 Jun;46(6):1073-83. doi: 10.1111/j.1365-313X.2006.02758.x. Huang WE, Wang H, Zheng H, Huang L, Singer AC, Thompson I, Whiteley AS. Chromosomally located gene fusions constructed in Acinetobacter sp. ADP1 for the detection of salicylate. Environ Microbiol. 2005 Sep;7(9):1339-48. doi: 10.1111/j.1462-5822.2005.00821.x. Single Cell Biology Xu J, Yi X, Jin G, Peng D, Fan G, Xu X, Chen X, Yin H, Cooper JM, Huang WE. High-Speed Diagnosis of Bacterial Pathogens at the Single Cell Level by Raman Microspectroscopy with Machine Learning Filters and Denoising Autoencoders. ACS Chem Biol. 2022 Feb 18;17(2):376-385. doi: 10.1021/acschembio.1c00834. Liang P, Liu B, Wang Y, Liu K, Zhao Y, Huang WE, Li B. Isolation and Culture of Single Microbial Cells by Laser Ejection Sorting Technology. Appl Environ Microbiol. 2022 Feb 8;88(3):e0116521. doi: 10.1128/AEM.01165-21. Zhang L, Li C, Peng D, Yi X, He S, Liu F, Zheng X, Huang WE, Zhao L, Huang X. Raman spectroscopy and machine learning for the classification of breast cancers. Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 5;264:120300. doi: 10.1016/j.saa.2021.120300. Wang J, Lin K, Hu H, Qie X, Huang WE, Cui Z, Gong Y, Song Y. In Vitro Anticancer Drug Sensitivity Sensing through Single-Cell Raman Spectroscopy. Biosensors (Basel). 2021 Aug 20;11(8). doi: 10.3390/bios11080286. Xu J, Yu T, Zois CE, Cheng JX, Tang Y, Harris AL, Huang WE. Unveiling Cancer Metabolism through Spontaneous and Coherent Raman Spectroscopy and Stable Isotope Probing. Cancers (Basel). 2021 Apr 5;13(7). doi: 10.3390/cancers13071718. Yi X, Song Y, Xu X, Peng D, Wang J, Qie X, Lin K, Yu M, Ge M, Wang Y, Zhang D, Yang Q, Wang M, Huang WE. Development of a Fast Raman-Assisted Antibiotic Susceptibility Test (FRAST) for the Antibiotic Resistance Analysis of Clinical Urine and Blood Samples. Anal Chem. 2021 Mar 30;93(12):5098-5106. doi: 10.1021/acs.analchem.0c04709. Xu J, Li X, Guo Z, Huang WE, Cheng JX. Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy. Anal Chem. 2020 Nov 3;92(21):14459-14465. doi: 10.1021/acs.analchem.0c02489. Ng CK, Xu J, Cai Z, Yang L, Thompson IP, Huang WE, Cao B. Elevated intracellular cyclic-di-GMP level in Shewanella oneidensis increases expression of c-type cytochromes. Microb Biotechnol. 2020 Nov;13(6):1904-1916. doi: 10.1111/1751-7915.13636. Hsu CC, Xu J, Brinkhof B, Wang H, Cui Z, Huang WE, Ye H. A single-cell Raman-based platform to identify developmental stages of human pluripotent stem cell-derived neurons. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18412-18423. doi: 10.1073/pnas.2001906117. Wang Y, Xu J, Kong L, Li B, Li H, Huang WE, Zheng C. Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota. Environ Microbiol. 2020 Jul;22(7):2613-2624. doi: 10.1111/1462-2920.14962. Wang Y, Xu J, Kong L, Liu T, Yi L, Wang H, Huang WE, Zheng C. Raman-deuterium isotope probing to study metabolic activities of single bacterial cells in human intestinal microbiota. Microb Biotechnol. 2020 Mar;13(2):572-583. doi: 10.1111/1751-7915.13519. Xu J, Preciado-Llanes L, Aulicino A, Decker CM, Depke M, Gesell Salazar M, Schmidt F, Simmons A, Huang WE. Single-Cell and Time-Resolved Profiling of Intracellular Salmonella Metabolism in Primary Human Cells. Anal Chem. 2019 Jun 18;91(12):7729-7737. doi: 10.1021/acs.analchem.9b01010. Liang B, Gao Y, Xu J, Song Y, Xuan L, Shi T, Wang N, Hou Z, Zhao YL, Huang WE, Chen ZJ. Raman profiling of embryo culture medium to identify aneuploid and euploid embryos. Fertil Steril. 2019 Apr;111(4):753-762.e1. doi: 10.1016/j.fertnstert.2018.11.036. Zhang S, Guo L, Yang K, Zhang Y, Ye C, Chen S, Yu X, Huang WE, Cui L. Induction of Escherichia coli Into a VBNC State by Continuous-Flow UVC and Subsequent Changes in Metabolic Activity at the Single-Cell Level. Front Microbiol. 2018;9:2243. doi: 10.3389/fmicb.2018.02243. Xu J, Potter M, Tomas C, Elson JL, Morten KJ, Poulton J, Wang N, Jin H, Hou Z, Huang WE. A new approach to find biomarkers in chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) by single-cell Raman micro-spectroscopy. Analyst. 2019 Jan 28;144(3):913-920. doi: 10.1039/c8an01437j. Jing X, Gou H, Gong Y, Su X, Xu, Ji Y, Song Y, Thompson IP, Xu J, Huang WE. Raman-activated cell sorting and metagenomic sequencing revealing carbon-fixing bacteria in the ocean. Environ Microbiol. 2018 Jun;20(6):2241-2255. doi: 10.1111/1462-2920.14268. Yuan X, Song Y, Song Y, Xu J, Wu Y, Glidle A, Cusack M, Ijaz UZ, Cooper JM, Huang WE, Yin H. Effect of Laser Irradiation on Cell Function and Its Implications in Raman Spectroscopy. Appl Environ Microbiol. 2018 Apr 15;84(8). doi: 10.1128/AEM.02508-17. Xu J, Zhu D, Ibrahim AD, Allen CCR, Gibson CM, Fowler PW, Song Y, Huang WE. Raman Deuterium Isotope Probing Reveals Microbial Metabolism at the Single-Cell Level. Anal Chem. 2017 Dec 19;89(24):13305-13312. doi: 10.1021/acs.analchem.7b03461. Song Y, Cui L, López JÁS, Xu J, Zhu YG, Thompson IP, Huang WE. Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River. Sci Rep. 2017 Nov 30;7(1):16648. doi: 10.1038/s41598-017-16898-x. Cui L, Yang K, Zhou G, Huang WE, Zhu YG. Surface-Enhanced Raman Spectroscopy Combined with Stable Isotope Probing to Monitor Nitrogen Assimilation at Both Bulk and Single-Cell Level. Anal Chem. 2017 Jun 6;89(11):5793-5800. doi: 10.1021/acs.analchem.6b04913. Xu J, Webb I, Poole P, Huang WE. Label-Free Discrimination of Rhizobial Bacteroids and Mutants by Single-Cell Raman Microspectroscopy. Anal Chem. 2017 Jun 20;89(12):6336-6340. doi: 10.1021/acs.analchem.7b01160. Tao Y, Wang Y, Huang S, Zhu P, Huang WE, Ling J, Xu J. Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy. Anal Chem. 2017 Apr 4;89(7):4108-4115. doi: 10.1021/acs.analchem.6b05051. Song Y, Kaster AK, Vollmers J, Song Y, Davison PA, Frentrup M, Preston GM, Thompson IP, Murrell JC, Yin H, Hunter CN, Huang WE. Single-cell genomics based on Raman sorting reveals novel carotenoid-containing bacteria in the Red Sea. Microb Biotechnol. 2017 Jan;10(1):125-137. doi: 10.1111/1751-7915.12420. Teng L, Wang X, Wang X, Gou H, Ren L, Wang T, Wang Y, Ji Y, Huang WE, Xu J. Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome. Sci Rep. 2016 Oct 19;6:34359. doi: 10.1038/srep34359. Wang Y, Song Y, Tao Y, Muhamadali H, Goodacre R, Zhou NY, Preston GM, Xu J, Huang WE. Reverse and Multiple Stable Isotope Probing to Study Bacterial Metabolism and Interactions at the Single Cell Level. Anal Chem. 2016 Oct 4;88(19):9443-9450. doi: 10.1021/acs.analchem.6b01602. Wang Y, Huang WE, Cui L, Wagner M. Single cell stable isotope probing in microbiology using Raman microspectroscopy. Curr Opin Biotechnol. 2016 Oct;41:34-42. doi: 10.1016/j.copbio.2016.04.018. Song Y, Yin H, Huang WE. Raman activated cell sorting. Curr Opin Chem Biol. 2016 Aug;33:1-8. doi: 10.1016/j.cbpa.2016.04.002. Cui L, Zhang YJ, Huang WE, Zhang BF, Martin FL, Li JY, Zhang KS, Zhu YG. Surface-Enhanced Raman Spectroscopy for Identification of Heavy Metal Arsenic(V)-Mediated Enhancing Effect on Antibiotic Resistance. Anal Chem. 2016 Mar 15;88(6):3164-70. doi: 10.1021/acs.analchem.5b04490. McIlvenna D, Huang WE, Davison P, Glidle A, Cooper J, Yin H. Continuous cell sorting in a flow based on single cell resonance Raman spectra. Lab Chip. 2016 Apr 21;16(8):1420-9. doi: 10.1039/c6lc00251j. Zhang P, Ren L, Zhang X, Shan Y, Wang Y, Ji Y, Yin H, Huang WE, Xu J, Ma B. Raman-activated cell sorting based on dielectrophoretic single-cell trap and release. Anal Chem. 2015 Feb 17;87(4):2282-9. doi: 10.1021/ac503974e. Zhang D, Berry JP, Zhu D, Wang Y, Chen Y, Jiang B, Huang S, Langford H, Li G, Davison PA, Xu J, Aries E, Huang WE. Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community. ISME J. 2015 Mar;9(3):603-14. doi: 10.1038/ismej.2014.161. Berry D, Mader E, Lee TK, Woebken D, Wang Y, Zhu D, Palatinszky M, Schintlmeister A, Schmid MC, Hanson BT, Shterzer N, Mizrahi I, Rauch I, Decker T, Bocklitz T, Popp J, Gibson CM, Fowler PW, Huang WE, Wagner M. Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):E194-203. doi: 10.1073/pnas.1420406112. Huang WE, Song Y, Xu J. Single cell biotechnology to shed a light on biological 'dark matter' in nature. Microb Biotechnol. 2015 Jan;8(1):15-6. doi: 10.1111/1751-7915.12249. Wang T, Ji Y, Wang Y, Jia J, Li J, Huang S, Han D, Hu Q, Huang WE, Xu J. Quantitative dynamics of triacylglycerol accumulation in microalgae populations at single-cell resolution revealed by Raman microspectroscopy. Biotechnol Biofuels. 2014;7:58. doi: 10.1186/1754-6834-7-58. Wang Y, Ji Y, Wharfe ES, Meadows RS, March P, Goodacre R, Xu J, Huang WE. Raman activated cell ejection for isolation of single cells. Anal Chem. 2013 Nov 19;85(22):10697-701. doi: 10.1021/ac403107p. Li M, Huang WE, Gibson CM, Fowler PW, Jousset A. Stable isotope probing and Raman spectroscopy for monitoring carbon flow in a food chain and revealing metabolic pathway. Anal Chem. 2013 Feb 5;85(3):1642-9. doi: 10.1021/ac302910x. Li M, Xu J, Romero-Gonzalez M, Banwart SA, Huang WE. Single cell Raman spectroscopy for cell sorting and imaging. Curr Opin Biotechnol. 2012 Feb;23(1):56-63. doi: 10.1016/j.copbio.2011.11.019. Li M, Canniffe DP, Jackson PJ, Davison PA, FitzGerald S, Dickman MJ, Burgess JG, Hunter CN, Huang WE. Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities. ISME J. 2012 Apr;6(4):875-85. doi: 10.1038/ismej.2011.150. Li M, Ashok PC, Dholakia K, Huang WE. Raman-activated cell counting for profiling carbon dioxide fixing microorganisms. J Phys Chem A. 2012 Jun 28;116(25):6560-3. doi: 10.1021/jp212619n.