研究领域

Physical & Theoretical Chemistry

Most recent information on work in the group can be found on our website. My group is interested in understanding the assembly and function of membrane-proteins using techniques that are capable of observing individual molecules. In general, our understanding of biomolecules is derived from experiments where the average properties of a population are measured. By using techniques capable of resolving single molecules it is possible to see more than just these ensemble-averaged attributes. For example, we can measure the distribution of a particular molecular property, or the reaction pathway followed by an individual molecule. The complexity of biological molecules, in both their underlying dynamic structure and their interactions, makes this single-molecule approach particularly valuable. We are interested in using these single-molecule methods to help us how membrane protein complexes form, the role of the lipid membrane in affecting the behaviour of membrane proteins, and how small chemical liganads can influence the conformation of membrane-bound receptors. Our principal tools are single-molecule laser microscopy and single-channel electrical recording. We use molecular biology to engineer proteins with fluorescent markers. These labelled proteins are then interrogated under the microscope. We collect data from many tens of thousands of molecules, and interpreting this data often requires the development of new computational tools and models.

近期论文

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High-Speed Single-Particle Tracking of GM1 in Model Membranes Reveals Anomalous Diffusion due to Interleaflet Coupling and Molecular Pinning. Spillane KM, Prtega-Arroyo J, de Wit G, Eggeling C, Ewers H, Wallace MI, Kukura P. Nano Lett. (2014) DOI: 10.1021/nl502536u JMB Photobleaching Reveals Heterogeneous Stoichiometry for Equinatoxin II Oligomers. Baker MAB, Rojko N, Cronin B, Anderluh G, Wallace MI. ChemBioChem (2014) DOI: 10.1002/cbic.201300799 JMB Combining Single-Molecule Imaging and Single-Channel Electrophysiology. Weatherill EE, Wallace MI J Mol Biol (2014) DOI:10.1016/j.jmb.2014.07.007 Marcoux Fluorescence Imaging of MACPF/CDC Proteins: New Techniques and Their Application. Senior MJ, Wallace MI Subcell Biochem (2014) 80, 293. Marcoux Mass spectrometry defines the C-terminal dimerization domain and enables modeling of the structure of full-length OmpA. Marcoux J, Politis A, Rinehart D, Marshall DP, Wallace MI, Tamm LK, Robinson CV. Structure (2014) 22, 781. EqtII Imaging the lipid phase-dependent pore formation of Equinatoxin II in Droplet Interface Bilayers. Rojko N, Cronin B, Danial JSH, Baker MAB, Anderluh G, Wallace MI Biophys J (2014) 106, 1630. alm Live cell imaging shows reversible assembly of the TatA component of the twin-arginine protein transport system. Alcock F, Baker MAB, Greene NP, Palmer T, Wallace MI, Berks BC PNAS (2013) dx.doi.org/10.1073/pnas.1306738110 alm A radical sense of direction: signalling and mechanism in cryptochrome magnetoreception. Dodson C, Hore PJ, Wallace MI TIBS (2013) dx.doi.org/10.1016/j.tibs.2013.07.002 alm Constructing droplet interface bilayers from the contact of aqueous droplets in oil. Leptihn S, Castell OK, Cronin B, Lee E, Gross LCM, Marshall DP, Thompson JR, Holden M, Wallace MI Nature Protocols (2013) 8 1048 alm One-step synthesis of fluorescein modified nano-carbon for Pd(II) detection via fluorescence quenching Panchompoo J, Aldous L, Baker M, Wallace MI, Compton RG. Analyst (2012) 137 2054 alm Quantification of Membrane Protein Inhibition by Optical Ion Flux in a Droplet Interface Bilayer Array Castell OK, Berridge J, Wallace MI. Angew.Chem.Int.Ed. (2012) 51 3134 alm Rapid Assembly of a Multimeric Membrane Protein Pore Thompson JR, Cronin B, Bayley H, Wallace MI. BiophysJ (2011) 101 2679 alm Determining Membrane Capacitance by Dynamic Control of Droplet Interface Bilayer Area Gross LCM, Heron AJ, Baca S, Wallace MI. Langmuir (2011) 27 14335 alm Imaging Multiple Conductance States in an Alamethicin Pore Harriss LM, Cronin B, Thompson JR, Wallace MI. JACS (2011) DOI: 10.1021/ja204275t concentration Dynamic and Reversible Control of 2D Membrane Protein Concentration in a Droplet Interface Bilayer Gross LCM, Castell OK, Wallace MI. Nano Letters (2011) DOI: 10.1021/nl201689v reconst In Vitro Reconstitution of Eukaryotic Ion Channels Using Droplet Interface Bilayers Leptihn S, Thompson JR, Ellory JC, Tucker SJ, Wallace MI. JACS (2011) dx.doi.org/10.1021/ja200128n NAR Visualizing helicases unwinding DNA at the single molecule level Fili N, Mashanov G, Toseland C, Batters C, Wallace MI, Yeeles J, Dillingham M, Webb M, Molloy J. Nucleic Acids Research (2010) lucky Lucky Imaging: Improved Localization Accuracy for Single Molecule Imaging Cronin B, de Wet B, Wallace MI. Biophys.J. (2009) 96, 2912. smfscr Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores Heron A, Thompson JR, Cronin B, Bayley H, Wallace MI. JACS. (2009) 131, 1652. singlemoleculebiology Single Molecule Fluorescence in Membrane Biology Harriss LM, Wallace MI. Single Molecule Biology ed. AE Knight,(2008) Academic Press ISBN-10:0123742277