吴边 - 中国科学院微生物研究所 -

个人简介

吴边，博士，中国科学院微生物研究所研究员、博士生导师，国家重点研发计划合成生物学项目首席，获国家优秀青年基金资助。主要工作致力于生物催化相关的元件挖掘、机理解析、酶工程改造、合成设计等工作；解析了酶催化碳-氮成键反应的详细机理，并通过人工改造将其应用于生物大分子与生物小分子的精准合成与定向修饰。尤其是近年来，将蛋白质计算机设计的前沿方法引入酶工程的研究中，促进了复杂大分子结构设计的发展。在Nat Chem Biol、Angew Chem、ACS Catalysis等国际主流学术刊物上发表论文数十篇。在此基础上构建出一系列化学品的生物合成途径，已有多肽药物酶法拼装、β-氨基酸生物合成、氮杂环类药物中间体顺次发酵等多项技术成功实现产业化应用。教育经历 2000年-2004年北京大学药学本科 2004年-2006年荷兰格罗宁根大学硕士 2006年-2010年荷兰格罗宁根大学博士工作经历 2010年-2012年荷兰格罗宁根大学/DSM 博士后 2012年-2014年荷兰格罗宁根大学研究组长 Enzypep B.V. 主任生物学家

研究领域

生命体中绝大多数催化功能由酶来实现，酶是合成生物学中的最核心的元件之一。从合成生物学的角度来认知生命，其本质是可数据化与可设计性。序列决定构像，而构像则决定功能，基于空间结构的酶序列数字化设计是近年重要的研究热点和前沿领域。本课题组面向工业生物催化创制需求，主要开展新生化反应设计与新酶改造研究，通过计算机蛋白质设计技术搭建具备广泛应用性、智能规划性的生物催化剂改造方案，以期解析理解蛋白质催化功能的基本规律，并指导研发新一代的工业绿色催化剂

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Feng J , Li RF, Zhang SS, Bu YF, Chen YC, Cui YL, Lin BX, Chen YH, Tao Y, Wu B*.Bioretrosynthesis of functionalized N-heterocycles from glucose via one-pot tandem collaborations of designed microbes. Adv. Sci. 2020. doi: 10.1002/advs.202001188. Li T, Cui XX, Cui YL, Sun JY, Chen YC, Zhu T, Li CJ, Li RF, Wu B*. Exploration of transaminase diversity for the oxidative conversion of natural amino acids into 2-keto acids and high-value chemicals. ACS . Catal. 2020,10(14):7950–7957. doi:10.1021/acscatal.0c01895. Zhu T, Li RF, Sun JY, Cui YL, Wu B*. Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir. Int. J. B iol. Macromol. 2020,(159):622–629. doi:10.1016/j.ijbiomac.2020.05.021. Li T, Li RF, Zhu T, Cui XX, Li CJ, Cui YL, Wu B*.Improving the system performance of the asymmetric biosynthesis of D‑pantoic acid by using artificially self-assembled enzymes in escherichia coli.ACS. Biomater. Sci. Eng. 2020,6(1):219–224. doi:10.1021/acsbiomaterials.9b01754. Mu QX, Cui YL, Tian YE, Hu MR, Tao Y, Wu B*. Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design. Int. J. Biol. Macromol. 2019, 136: 1060-1068. doi: 10.1016/j.ijbiomac.2019.06.094. Zheng XX, Cui YL, Li T, Li RF, Guo L, Li DF, Wu B*. Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acid. Appl. Microbiol. Biotech. 2019, 103(19): 8051-8062. doi: 10.1007/s00253-019-10105-9. Li T, Cui YL, Wu B*. Molecular dynamics investigations of structural and functional changes in Bcl-2 induced by the novel antagonist BDA-366. J. Biomol. Struct. & Dyn. 2019, 37(10): 2527-2537. doi:10.1080/07391102.2018.1491424. Li RF, Wijma HJ, Song L, Cui YL, Otzen M, Tian YE, Du JW, Li T, Niu DD, Chen YC, Feng J, Han J, Chen H, Tao Y, Janssen DB*, Wu B*. Computational redesign of enzymes for regio- and enantioselective hydroamination. Nat. Chem. Biol. 2018, 14(7): 664-670. doi: 10.1038/s41589-018-0053-0. Bu YF, Cui YL, Peng Y, Hu MR, Tian Y’E, Tao Y, Wu B*. Engineering improved thermostability of the GH11 xylanase from Neocallimastix patriciarum via computational library design. Appl. Microbiol. Biotech. 2018, 102(8): 3675-3685. doi: 10.1007/s00253-018-8872-1. Zhu T, Song L, Li RF, Wu B*. Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase. Chin. Chem. Lett. 2018, 29(7): 1116-1118. doi: 10.1016/j.cclet.2018.03.033. Tian XY, He GJ, Hu PJ, Chen L, Tao CY, Cui YL, Shen L, Ke WX, Xu HJ, Zhao YB, Xu QJ, Bai FY, Wu B, Yang E, Lin XR, Wang LQ *. Cryptococcus neoformans sexual reproduction is controlled by a quorum sensing peptide. Nat. Microbiol. 2018, 3(6): 698-707. doi: 10.1038/s41564-018-0160-4. Feng J, Zhang P, Cui YL, Li K, Qiao X, Zhang YT, Li SM, Cox R.J, Wu B, Ye M*, Yin WB *. Regio- and stereospecific O-glycosylation of phenolic compounds catalyzed by a fungal glycosyltransferase from Mucor hiemalis. Adv. Synth. Catal. 2017, 359(6): 995-1006. doi: 10.1002/adsc.201601317. Wu B *, Wijma HJ, Song L, Rozeboom HJ, Poloni C, Tian YE, Arif MI, Nuijens T, Quaedflieg PJ, Szymanski W, Feringa BL, Janssen DB*. Versatile peptide C-terminal functionalization via a computationally engineered peptide amidase. ACS.Catal. 2016, 6(8): 5405-5414. doi: 10.1021/acscatal.6b01062. Toplak A, Nuijens T, Quaedflieg PJ, Wu B*, Janssen DB*. Peptiligase, an enzyme for efficient chemoenzymatic peptide synthesis and cyclization in water. Adv. Synth. Catal . 2016, 358(13): 2140-2147. doi: 10.1002/adsc.201600017. Nuijens T , Toplak A , Quaedflieg PJLM , Drenth J , Wu B*, Janssen DB*. Engineering a diverse ligase toolbox for peptide segment condensation. Adv. Synth. Catal . 2016, 358(24): 4041-4048. doi: 10.1002/adsc.201600774. Wu B, Toplak A, Nijens T, Queadflieg P, van der Laan, JM, Janssen DB “PEPTIDE FRAGMENT CONDENSATION USING A SUBTILISIN VARIANT WITH IMPROVED SYNTHESIS OVER HYDROLYSIS RATIO” International Patent Application No. PCT/NL2014/050707