个人简介

I am interested in all aspects of how bacteria pass on their genetic information, from DNA replication to chromosome segregation and accurate cell division. My current research focuses on two main topics. Firstly what happens when the process of DNA replication runs into a blockage and stops- how can the cell recover to restart the vital process of copying its DNA? A large number of homologous recombination proteins have been implicated in the processing of collapsed DNA replication forks and their roles, and the pathways used, will be investigated in living cells. Secondly, is the study of the FtsK protein, which co-ordinates the processes of cell division, chromosome unlinking and chromosome segregation in bacteria. Each of these processes has to be completed in a timely manner to allow the cell to divide to produce offspring with a full genetic content. The DNA translocase protein, FtsK, is a key protein in each of these processes, and could additionally act as a cell division checkpoint. Post-doctoral work: Department of Biochemistry, University of Oxford (2005-2009) Cancer Research UK (2000-2004) University of Texas at Austin (1997-2000) PhD: University of Oxford, UK (1994-1997)

研究领域

Biological Sciences

I am interested in all aspects of how bacteria pass on their genetic information, from DNA replication to chromosome segregation and accurate cell division. My current research focuses on two main topics. Firstly what happens when the process of DNA replication runs into a blockage and stops- how can the cell recover to restart the vital process of copying its DNA? A large number of homologous recombination proteins have been implicated in the processing of collapsed DNA replication forks and their roles, and the pathways used, will be investigated in living cells. Secondly, is the study of the FtsK protein, which co-ordinates the processes of cell division, chromosome unlinking and chromosome segregation in bacteria. Each of these processes has to be completed in a timely manner to allow the cell to divide to produce offspring with a full genetic content. The DNA translocase protein, FtsK, is a key protein in each of these processes, and could additionally act as a cell division checkpoint. Post-doctoral work: Department of Biochemistry, University of Oxford (2005-2009) Cancer Research UK (2000-2004) University of Texas at Austin (1997-2000) PhD: University of Oxford, UK (1994-1997)

近期论文

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2016 Mettrick KA, Lawrence N, Mason C, Weaver GM, Corocher TA, Grainge I, 'Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System.', Journal of visualized experiments : JoVE, (2016) 2016 Guo P, Noji H, Yengo CM, Zhao Z, Grainge I, 'Biological Nanomotors with a Revolution, Linear, or Rotation Motion Mechanism.', Microbiol Mol Biol Rev, 80 161-186 (2016) 2015 Mettrick KA, Grainge I, 'Stability of blocked replication forks in vivo', Nucleic Acids Research, 44 657-668 (2015) [C1] 2014 Guo P, Grainge I, Zhao Z, Vieweger M, 'Two classes of nucleic acid translocation motors: Rotation and revolution without rotation', Cell and Bioscience, 4 (2014) [C1] 2013 Shimokawa K, Ishihara K, Grainge I, Sherratt DJ, Vazquez M, 'FtsK-dependent XerCD-dif recombination unlinks replication catenanes in a stepwise manner.', Proc Natl Acad Sci U S A, 110 20906-20911 (2013) [C1] 2013 Grainge I, 'Simple topology: FtsK-directed recombination at the dif site', BIOCHEMICAL SOCIETY TRANSACTIONS, 41 595-600 (2013) [C1] 2011 Grainge IR, Lesterlin C, Sherratt DJ, 'Activation of XerCD-dif recombination by the FtsK DNA translocase', Nucleic Acids Research, 39 5140-5148 (2011) [C1] 2010 Crozat E, Meglio A, Allemand J-F, Chivers CE, Howarth M, Venien-Bryan C, et al., 'Separating speed and ability to displace roadblocks during DNA translocation by FtsK.', The EMBO Journal, 29 1423-1433 (2010) [C1] 2010 Sherratt DJ, Arciszewska L, Crozat E, Graham J, Grainge IRF, 'The Escherichia coli DNA translocase FtsK.', Biochemical Society Transactions, 38 395-398 (2010) [C1] 2010 Grainge IR, 'FtsK - a bacterial cell division checkpoint?', Molecular Microbiology, 78 1055-1057 (2010) [C1] 2010 Crozat E, Grainge IR, 'FtsK DNA translocase: The fast motor that knows where it's going', ChemBioChem, 11 2232-2243 (2010) [C1] 2008 Lowe J, Ellonen A, Allen MD, Atkinson C, Sherratt DJ, Grainge IRF, 'Molecular mechanism of sequence-directed DNA loading and translocation by FtsK', Molecular Cell, 31 498-509 (2008) [C1] 2008 Grainge IRF, 'Sporulation: SpoIIIE is the key to cell differentiation', Current Biology, 18 871-872 (2008) [C1] 2008 Atanassova N, Grainge IRF, 'Biochemical Characterization of the Minichromosome Maintenance (MCM) Protein of the Crenarchaeote Aeropyrum pernix and Its Interactions with the Origin Recognition Complex (ORC) Proteins', Biochemistry, 47 1336-1337 (2008) [C1] 2007 Grainge I, Sherratt DJ, 'Site-specific recombination', Topics in Current Genetics, 17 27-52 (2007) 2007 Grainge I, Bregu M, Vazquez M, Sivanathan V, Ip SCY, Sherratt DJ, 'Unlinking chromosome catenanes in vivo by site-specific recombination', EMBO JOURNAL, 26 4228-4238 (2007) [C1] 2006 Grainge I, Gaudier M, Schuwirth BS, Westcott SL, Sandall J, Atanassova N, Wigley DB, 'Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernix', JOURNAL OF MOLECULAR BIOLOGY, 363 355-369 (2006) [C1] 2006 Possoz C, Filipe SR, Grainge I, Sherratt DJ, 'Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo', EMBO JOURNAL, 25 2596-2604 (2006) [C1] 2004 Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB, 'Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix', JOURNAL OF MOLECULAR BIOLOGY, 343 547-557 (2004) [C1] 2003 Grainge I, Scaife S, Wigley DB, 'Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus', NUCLEIC ACIDS RESEARCH, 31 4888-4898 (2003) [C1]