个人简介

M.Sc. Biological Engineering, University of Birmingham, England; Ph.D. in Molecular Microbiology, University of Limerick, Ireland, and Graduate Certificate of Higher Education, (GCHE) Monash University, Australia

研究领域

Microbiology

Molecular microbiology: Characterization of the virulence factors in the Australian Epidemic Strains (AES) of Pseudomonas aeruginosa - The project involves molecular genotyping of sixty two clinical isolates of P.aeruginosa using Ribotyping, ERIC and BOX PCR, as well as Pulsed Field Gel Electrophoresis (PFGE) . In addition, the expression levels of several virulence factors; e.g. elastases, alkaline protease and protease IV as well as the blue pigment pyocyanin, are also profiled. Real-time PCR is also used to measure the expression level of the elastase gene from the clinical isolates at the level of transcription having determined the elastase mRNA life. Role of cytokines in inflammatory joint diseases (Arthritis): Evaluation of IL-6 cytokine subfamily antagonists for he treatment of Inflammatory arthritis (ongoing). This project is investigating the role of two pro inflammatory cytokines; leukaemia inhibitory factor (LIF) and oncostatin M (OSM), in articular cartilage degradation. We have used both human and porcine primary chondrocytes as well as established cell lines, to investigating the role of LIF and OSM on a range of matrix metalloproteinases (MMPs) release leading to articular cartilage degradation. In addition, the project is evaluating two potential antagonists LIF receptor antagonist to block the pro inflammatory activities of LIF and OSM. The genes encoding the protein antagonists were cloned, expressed in E.coli, purified and their biological activities determined using cell based assays. Additionally, to prolong the serum half life of the antagonists their genes were fused to Fc region of human IgG1. The in vivo studies associated with this project included the pharmacokinetics studies, to determine the antagonists serum stability, half-life and toxicities. DNA vaccines: We are using attenuated Listeria monocytogenes to deliver DNA vaccines to undifferentiated epithelial cells. The project has already generated a recombinant stable, suicidal Listeria monocytogenes strain capable of delivering antigens as protein or DNA into non diving intestinal epithelial cells. We are now testing the construct in vivo to determine the antibody immune cell (T-cell) response. Gene therapy: This project is focusing on the design and valuation of a range of novel gene delivery agents. For this purpose, we designed a series of lipopeptide as transfection agents, consisting of an alkyl chain, one cysteine, and various other amino acid residues, viz., 1 to 4 histidines and 1 to 3 lysines. These constructs are being evaluated for their biophysical properties and transfection efficiencies. Breast Cancer: Progesterone membrane receptor component 1/ sigma 2 receptor (PGRMC1/S2R) is associated with the regulation of various cellular processes, including lipid metabolism and apoptosis. In addition, PGRMC1/S2R plays a role in tumourigenesis and promoting cell survival of human cancer cells following chemotherapeutic treatment. The cellular mechanisms and pathways leading to tumourigenesis are not fully understood. Previous work has identified that this protein is differently phosphorylated in breast cancers differing in estrogen receptor status. The aim of this project is to characterize this further using mass spectrometry and confocal microscopy.

近期论文

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Ann Pallis, Jalal A.Jazayeri, Peter Ward, Karolina Dimovski, and Suzanne Svobodova, Rapid detection of Clostridium difficile toxins from stool samples using Real-Time Multiplex PCR (2013) Journal of Medical Microbiology (accepted) Hunt LC, Upadhyay A, Jazayeri JA, Tudor EM, White JD, An anti-inflammatory role for leukemia inhibitory factor receptor signalling in regenerating skeletal muscle, 2012, Histochemistry and Cell Biology (2013), Volume 139, Issue 1, pp 13-34 Upadhyay, Aradhana; Senychyn, Danielle; Gu, Ran; Santos, Leilani; Carroll, Graeme; Jazayeri, Jalal, K/BxN serum transfer arthritis is delayed and less severe in leukaemia inhibitory factor (LIF) deficient mice, Clinical and Experimental Immunology (2012), Vol 162(2) 71-8) Jazayeri JA, Carroll GJ, (Book Chapter, pp157-188) Half-life extension by fusion to the Fc region in Therapeutic Proteins: Strategies to modulate their plasma half-life. John Wiley (2012), ISBN 978-3-527-32849-9 Hunt LC, Upadhyay A, Jazayeri JA, Tudor EM and White JD Myogenic transcription factors and cell cycle inhibitors are regulated by leukemia inhibitory factor to mediate inhibition of myogenic differentiation. Skeletal Muscle (2011); 17 Jazayeri JA, Carroll GJ, Vernallis AB. Targeting the gp130 receptor subunit to control pain and inflammation, Journal of Interferon and cytokine Research, (2010) Vol 30,2 865-873 Jazayeri JA, Carroll GJ, Vernallis AB. Interleukin-6 subfamily cytokines and rheumatoid arthritis: Role of antagonists. Int Immunopharmacol (2010);10:1-8. Carroll G, upadhyay A, Sharma G, Jazayeri J. Ferritin concentrations in synovial fluid are higher in Osteoarthritis (OA) patients with HFE gene mutations (C282Y or H63D). Scandinavian Journal of Rheumatology (2010) 39,5 pp413-420 Upadhyay A, Sharma G, Kivivuori S, Raye WS, Zabihi E, Carroll GJ, Jazayeri JA. Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes. Cytokine (2009);46:332-338. Mozafari MR, Pardakhty A, Azarmi S, Jazayeri JA, Nokhodchi A, Omri A. Role of nanocarrier systems in cancer nanotherapy. J Liposome Res (2009);19:310-321. Kuo CY, Sinha S, Jazayeri JA, Pouton CW. A stably engineered, suicidal strain of listeria monocytogenes delivers protein and/or DNA to fully differentiated intestinal epithelial monolayers. Molecular Pharmacology (2009);6:1052-1061. Carroll GJ, Breidahl WH, Jazayeri JA. Confirmation of two major polyarticular osteoarthritis (POA) phenotypes--differentiation on the basis of joint topography. Osteoarthritis Cartilage (2009);17:891-895. Tarwadi, Jazayeri JA, Prankerd RJ, Pouton CW. Preparation and in vitro evaluation of novel lipopeptide transfection agents for efficient gene delivery. Bioconjug Chem 2008;19:940-950. Jazayeri JA, Carroll GJ. Fc-based cytokines : prospects for engineering superior therapeutics. BioDrugs (2008);22:11-26. Jazayeri JA, De Weerd N, Raye W, Velkov T, Santos L, Taylor D, Carroll GJ. Generation of mutant leukaemia inhibitory factor (LIF)-IgG heavy chain fusion proteins as bivalent antagonists of LIF. J Immunol Methods (2007);323:1-10. Jazayeri JA, De Weerd N, Raye W, Kivivuori S, Zabihi E, Carroll GJ. In vitro evaluation of leukemia inhibitory factor receptor antagonists as candidate therapeutics for inflammatory arthritis. J Interferon Cytokine Res (2007);27:281-289. Jazayeri JA. Biochemical studies on sheep body louse Bovicola Ovis, comparison of pyrethroid and organophosphate resistant and susceptible strains. Pesticide Biochemistry and Physiology (2004);80:1-11. Giles KM, Daly JM, Beveridge DJ, Thomson AM, Voon DC, Furneaux HM, Jazayeri JA, Leedman PJ. The 3'-untranslated region of p21WAF1 mRNA is a composite cis-acting sequence bound by RNA-binding proteins from breast cancer cells, including HuR and poly(C)-binding protein. J Biol Chem (2003);278:2937-2946. Balmer LA, Beveridge DJ, Jazayeri JA, Thomson AM, Walker CE, Leedman PJ. Identification of a novel AU-Rich element in the 3' untranslated region of epidermal growth factor receptor mRNA that is the target for regulated RNA-binding proteins. Mol Cell Biol (2001);21:2070-2084. Seth D, Shaw K, Jazayeri JA, Leedman PJ. Complex post-transcriptional regulation of EGF-receptor expression by EGF and TGF-alpha in human prostate cancer cells. British J Cancer (1999);80:657-669.