研究领域

Characterisation of plant viruses Transgenic control of plant pathogens Molecular farming

His research initially focussed on the characterisation of plant viruses and the development of diagnostics, and from this work he has identified, characterised and patented a suite of promoters for transgene expression. During the last 10 years, his research focus has been directed towards the control of important viral (potyviruses, geminiviruses, nanoviruses, caulimoviruses and reoviruses) and fungal (Fusarium) pathogens affecting tropical/subtropical plants (banana, sugarcane, sweetpotato, taro and yam), particularly through the development of transgenic disease resistance. A variety of different conventional and novel strategies have been utilised including gene silencing (viruses), and the use of plant R genes and stress tolerance genes. As part of a research team, he has developed a range of biotechnology methods and products for expressing genes in transgenic plants, a number of which have been patented. For example, he is a co–inventor of InPAct, a novel platform technology for controlled, high level transgene expression in plants which has widespread applications ranging from disease resistance to molecular farming. He has also been involved in research and development programs in south–east Asia, including R&D programs involving sweetpotato and taro viruses. More recently, he has been involved in a Bill and Melinda Gates-funded project which aims to generate micronutrient-biofortified bananas for East Africans.

近期论文

查看导师新发文章 （温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Dugdale B, Mortimer CL, Kato M, James T, Harding R, Dale J, (2014) Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants, Nature Protocols p1010-1027 Dugdale B, Mortimer CL, Kato M, James TA, Harding R, Dale J, (2013) In plant activation: An inducible, hyperexpression platform for recombinant protein production in plants, The Plant Cell p2429-2443 Mlalazi B, Welsch R, Namanya P, Khanna HK, Geijskes J, Harrison MD, Harding R, Dale J, Bateson M, (2012) Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes, Planta: an international journal of plant biology p1585-1598 Paul J, Becker DK, Dickman MB, Harding R, Khanna HK, Dale J, (2011) Apoptosis-related genes confer resistance to Fusarium wilt in transgenic 'Lady Finger' bananas, Plant Biotechnology Journal p1141-1148 James AP, Geijskes RJ, Dale J, Harding R, (2011) Development of a novel rolling-circle amplification technique to detect Banana streak virus that also discriminates between integrated and episomal virus sequences, Plant Disease: an international journal of applied plant pathology p57-62 Peraza Echeverria S, Dale J, Harding R, Collet CC, (2009) Molecular cloning and in silico analysis of potential Fusarium resistance genes in banana, Molecular Breeding p431-443 Deo PC, Harding R, Taylor M, Tyagi AP, Becker DK, (2009) Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta), Plant cell, tissue and organ culture p61-71 Peraza Echeverria S, Dale J, Harding R, Smith MK, Collet CC, (2008) Characterization of disease resistance gene candidates of the nucleotide binding site (NBS) type from banana and correlation of a transcriptional polymorphism with resistance to Fusarium oxysporum f.sp. cubense race 4, Molecular Breeding: new strategies in plant improvement p565-579 Ha V, Coombs S, Revill PA, Harding R, Vu M, Dale J, (2008) Molecular characterization of begomoviruses and DNA satellites from Vietnam: additional evidence that the New World geminiviruses were present in the Old World prior to continental separation, Journal of General Virology p312-326 Khanna HK, Paul JY, Harding RM, Dickman M, Dale JL, (2007) Inhibition of Agrobacterium-Induced Cell Death by Antiapoptotic Gene Expression Leads to Very High Transformation Efficiency of Banana, Molecular Plant-Microbe Interactions p1048-1054