Mohammed, Shabaz - University of Oxford

个人简介

Shabaz studied Chemistry at UMIST (now The University of Manchester) and obtained his degree in 1999. He then went on to work in the laboratory of Simon Gaskell within the field of biological mass spectrometry albeit the more fundamental side of the topic and defended in early 2003. He then moved to Odense (Denmark) and joined the group of Ole Jensen and worked on technology development for use in studying post-translation modifications. Here, he helped develop a method that allowed quantitative analysis of phosphorylation and its role in cellular signalling, a widely adopted method. In 2005, he moved to the laboratory of Albert Heck and continued working in the field of proteomic technologies. In 2008, he became an Assistant Professor and started his own group. In 2013, he (once again) moved and is now an Associate Professor of Proteomics in the Departments of Chemistry and Biochemistry. He also maintains a ‘guest’ lecturer status at Utrecht University.

研究领域

Proteins are central to many essential molecular processes in living cells. They perform functions through interactions in a highly dynamic environment with each other and with other types of molecules including sugars, lipids and nucleic acids. In recent years, characterisation of proteins has accelerated dramatically, driven by the completion of many genomes and the constant evolution of mass spectrometry. The genome contains information relating to all possible proteins that can be present in the cell and thus this extractable data represents an invaluable repository of knowledge for protein identification. Equally significant was the realisation that mass spectrometry represents an almost ideal tool for protein sequencing. However, the composition of proteins present in a cell is only one determinant of function. Both protein expression levels and subtle biochemical changes to protein structure, in the form of post-translational modifications (PTM), dynamically influence cellular processes. PTM of proteins is nature’s way to regulate protein function, their interactions and sub-cellular localisation. Furthermore, proteins involved in regulation are becoming major drug targets for a wide variety of diseases. Therefore, the accurate measurement of protein expression and modification is essential to understanding the underlying biomolecular determinants of cellular signaling. Currently, I am involved in: (A) developing chromatographic techniques for application in a proteomics environment, (B) enrichment of post translational modifications, (C) characterization and exploitation of electron transfer dissociation for peptide sequencing, (D) creation of facile chemical labeling based quantification and (E) elucidation of (PTM based) signaling pathways through quantification

近期论文

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Select item 25515538 A systems-wide screen identifies substrates of the SCFβTrCP ubiquitin ligase. Low TY, Peng M, Magliozzi R, Mohammed S, Guardavaccaro D, Heck AJ. Sci Signal. 2014 Dec 16;7(356):rs8. doi: 10.1126/scisignal.2005882. PMID: 25515538 [PubMed - in process] Select item 25485597 Rapid Analyses of Proteomes and Interactomes Using an Integrated Solid-Phase Extraction-Liquid Chromatography-MS/MS System. Binai NA, Marino F, Soendergaard P, Bache N, Mohammed S, Heck AJ. J Proteome Res. 2015 Feb 6;14(2):977-85. doi: 10.1021/pr501011z. Epub 2014 Dec 8. PMID: 25485597 [PubMed - in process] Select item 25346955 Characterization and usage of the EASY-spray technology as part of an online 2D SCX-RP ultra-high pressure system. Marino F, Cristobal A, Binai NA, Bache N, Heck AJ, Mohammed S. Analyst. 2014 Dec 21;139(24):6520-8. doi: 10.1039/c4an01568a. PMID: 25346955 [PubMed - in process] Select item 25202122 USP17- and SCFβTrCP--regulated degradation of DEC1 controls the DNA damage response. Kim J, D'Annibale S, Magliozzi R, Low TY, Jansen P, Shaltiel IA, Mohammed S, Heck AJ, Medema RH, Guardavaccaro D. Mol Cell Biol. 2014 Nov 15;34(22):4177-85. doi: 10.1128/MCB.00530-14. Epub 2014 Sep 8. PMID: 25202122 [PubMed - indexed for MEDLINE] Select item 25068997 Characterization of biases in phosphopeptide enrichment by Ti(4+)-immobilized metal affinity chromatography and TiO2 using a massive synthetic library and human cell digests. Matheron L, van den Toorn H, Heck AJ, Mohammed S. Anal Chem. 2014 Aug 19;86(16):8312-20. doi: 10.1021/ac501803z. Epub 2014 Aug 4. PMID: 25068997 [PubMed - in process] Select item 25000127 Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. Binai NA, Bisschops MM, van Breukelen B, Mohammed S, Loeff L, Pronk JT, Heck AJ, Daran-Lapujade P, Slijper M. J Proteome Res. 2014 Aug 1;13(8):3542-53. doi: 10.1021/pr5003388. Epub 2014 Jul 18. PMID: 25000127 [PubMed - in process] Select item 24665413 HGF signaling regulates Claudin-3 dynamics through its C-terminal tyrosine residues. Twiss F, Oldenkamp M, Hiemstra A, Zhou H, Matheron L, Mohammed S, de Rooij J. Tissue Barriers. 2013 Oct 1;1(4):e27425. doi: 10.4161/tisb.27425. Epub 2014 Jan 9. PMID: 24665413 [PubMed] Free PMC Article Select item 24500709 Proteasome-dependent degradation of transcription factor activating enhancer-binding protein 4 (TFAP4) controls mitotic division. D'Annibale S, Kim J, Magliozzi R, Low TY, Mohammed S, Heck AJ, Guardavaccaro D. J Biol Chem. 2014 Mar 14;289(11):7730-7. doi: 10.1074/jbc.M114.549535. Epub 2014 Feb 5. PMID: 24500709 [PubMed - indexed for MEDLINE] Free PMC Article Select item 24422449 Phosphoproteomics. Yates JR 3rd, Mohammed S, Heck AJ. Anal Chem. 2014 Feb 4;86(3):1313. doi: 10.1021/ac404019p. Epub 2014 Jan 15. No abstract available. PMID: 24422449 [PubMed - indexed for MEDLINE] Select item 24393126 Synthetic phosphorylation of p38α recapitulates protein kinase activity. Chooi KP, Galan SR, Raj R, McCullagh J, Mohammed S, Jones LH, Davis BG. J Am Chem Soc. 2014 Feb 5;136(5):1698-701. doi: 10.1021/ja4095318. Epub 2014 Jan 27. PMID: 24393126 [PubMed - indexed for MEDLINE] Free PMC Article Select item 24290981 Control of epithelial cell migration and invasion by the IKKβ- and CK1α-mediated degradation of RAPGEF2. Magliozzi R, Low TY, Weijts BG, Cheng T, Spanjaard E, Mohammed S, van Veen A, Ovaa H, de Rooij J, Zwartkruis FJ, Bos JL, de Bruin A, Heck AJ, Guardavaccaro D. Dev Cell. 2013 Dec 9;27(5):574-85. doi: 10.1016/j.devcel.2013.10.023. Epub 2013 Nov 27. PMID: 24290981 [PubMed - indexed for MEDLINE] Free Article Select item 24290761 Quantitative and qualitative proteome characteristics extracted from in-depth integrated genomics and proteomics analysis. Low TY, van Heesch S, van den Toorn H, Giansanti P, Cristobal A, Toonen P, Schafer S, Hübner N, van Breukelen B, Mohammed S, Cuppen E, Heck AJ, Guryev V. Cell Rep. 2013 Dec 12;5(5):1469-78. doi: 10.1016/j.celrep.2013.10.041. Epub 2013 Nov 27. PMID: 24290761 [PubMed - indexed for MEDLINE] Free Article Select item 23921808 The CRAPome: a contaminant repository for affinity purification-mass spectrometry data. Mellacheruvu D, Wright Z, Couzens AL, Lambert JP, St-Denis NA, Li T, Miteva YV, Hauri S, Sardiu ME, Low TY, Halim VA, Bagshaw RD, Hubner NC, Al-Hakim A, Bouchard A, Faubert D, Fermin D, Dunham WH, Goudreault M, Lin ZY, Badillo BG, Pawson T, Durocher D, Coulombe B, Aebersold R, Superti-Furga G, Colinge J, Heck AJ, Choi H, Gstaiger M, Mohammed S, Cristea IM, Bennett KL, Washburn MP, Raught B, Ewing RM, Gingras AC, Nesvizhskii AI. Nat Methods. 2013 Aug;10(8):730-6. doi: 10.1038/nmeth.2557. Epub 2013 Jul 7. PMID: 23921808 [PubMed - indexed for MEDLINE] Free PMC Article Select item 23909401 Collaboration of AMPK and PKC to induce phosphorylation of Ser413 on PIP5K1B resulting in decreased kinase activity and reduced PtdIns(4,5)P2 synthesis in response to oxidative stress and energy restriction. van den Bout I, Jones DR, Shah ZH, Halstead JR, Keune WJ, Mohammed S, D'Santos CS, Divecha N. Biochem J. 2013 Nov 1;455(3):347-58. doi: 10.1042/BJ20130259. PMID: 23909401 [PubMed - indexed for MEDLINE] Free Article Select item 23685481 A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. Marx H, Lemeer S, Schliep JE, Matheron L, Mohammed S, Cox J, Mann M, Heck AJ, Kuster B. Nat Biotechnol. 2013 Jun;31(6):557-64. doi: 10.1038/nbt.2585. Epub 2013 May 19. PMID: 23685481 [PubMed - indexed for MEDLINE] Select item 23612710 Comparative phosphoproteomic analysis of checkpoint recovery identifies new regulators of the DNA damage response. Halim VA, Alvarez-Fernández M, Xu YJ, Aprelia M, van den Toorn HW, Heck AJ, Mohammed S, Medema RH. Sci Signal. 2013 Apr 23;6(272):rs9. doi: 10.1126/scisignal.2003664. PMID: 23612710 [PubMed - indexed for MEDLINE] Select item 23605687 Characterization of electron transfer dissociation in the Orbitrap Velos HCD cell. Frese CK, Nolting D, Altelaar AF, Griep-Raming J, Mohammed S, Heck AJ. J Am Soc Mass Spectrom. 2013 Nov;24(11):1663-70. doi: 10.1007/s13361-013-0618-9. Epub 2013 Apr 20. PMID: 23605687 [PubMed - indexed for MEDLINE] Select item 23581470 Profiling of diet-induced neuropeptide changes in rat brain by quantitative mass spectrometry. Frese CK, Boender AJ, Mohammed S, Heck AJ, Adan RA, Altelaar AF. Anal Chem. 2013 May 7;85(9):4594-604. doi: 10.1021/ac400232y. Epub 2013 Apr 26. PMID: 23581470 [PubMed - indexed for MEDLINE] Select item 23510141 Universal quantitative kinase assay based on diagonal SCX chromatography and stable isotope dimethyl labeling provides high-definition kinase consensus motifs for PKA and human Mps1. Hennrich ML, Marino F, Groenewold V, Kops GJ, Mohammed S, Heck AJ. J Proteome Res. 2013 May 3;12(5):2214-24. doi: 10.1021/pr400074f. Epub 2013 Apr 3. PMID: 23510141 [PubMed - indexed for MEDLINE] Select item 23403867 Quantitative global phosphoproteomics of human umbilical vein endothelial cells after activation of the Rap signaling pathway. Meijer LA, Zhou H, Chan OY, Altelaar AF, Hennrich ML, Mohammed S, Bos JL, Heck AJ. Mol Biosyst. 2013 Apr 5;9(4):732-49. doi: 10.1039/c3mb25524g. PMID: 23403867 [PubMed - indexed for MEDLINE]