个人简介

After graduating from Sydney University Professor Ollis completed a Post-doctoral period at Yale and then held faculty position at Northwestern University. He has since held positions at Yale University (1980-1985), Northwestern University Evanston (1986-1991) before his appointment as Senior Fellow in the Research School of Chemistry. He was awarded a fellow of the American Institute of Chemistry (1987) and the Victor Chang Cardiac Research Institute Award (2005). He was promoted to Professor at the Research School of Chemistry in 2005.

研究领域

Protein crystallography & engineering

We study how proteins function and investigate how they might be modified for new and useful purposes. Our group uses directed evolution to produce mutant proteins which are then screened for enhanced function or other interesting properties. We frequently discover proteins which may be useful in industrial and environmental applications. X-ray crystallography and a variety of other techniques are used to determine the structures of proteins and better understand the detailed mechanics of protein function.

近期论文

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Porter, J, Rusli, R & Ollis, D 2016, 'Directed Evolution of Enzymes for Industrial Biocatalysis', ChemBioChem, vol. 17, no. 3, pp. 197-203. Pedroso, M, Larrabee, J, Ely, F et al 2016, 'CaII Binding Regulates and Dominates the Reactivity of a Transition-Metal-Ion-Dependent Diesterase from Mycobacterium tuberculosis', Chemistry, A European Journal, vol. 22, no. 3, pp. 999-1009. Schenk, G, Mateen, I, Ng, T et al 2016, 'Organophosphate-degrading metallohydrolases: Structure and function of potent catalysts for applications in bioremediation', Coordination Chemistry Reviews, vol. 317, pp. 122-131. Porter, J, Boon, P, Murray, T et al 2015, 'Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multi-directional search', ACS chemical biology, vol. 10, no. 2, pp. 611-621. Porter, J, Collyer, C & Ollis, D 2015, 'Compensatory Stabilizing Role of Surface Mutations During the Directed Evolution of Dienelactone Hydrolase for Enhanced Activity', Protein Journal, vol. 34, no. 1, pp. 82-89. Ng, T-K., Gahan, L, Schenk, G and Ollis, D.L.l 2015, 'Altering the substrate specificity of methyl parathion hydrolase with directed evolution', Archives of Biochemistry and Biophysics, vol. 573, pp. 59-68. Stevenson, B, Waller, C, Ma, P et al 2015, 'Pseudomonas aeruginosa arylsulfatase: A purified enzyme for the mild hydrolysis of steroid sulfates', Drug Testing and Analysis, (www.drugtestinganalysis.com) DOI 10.1002/dta.1782 Hou, C, Phelan, E, Miraula, M et al 2014, 'Unusual metallo-?2-lactamases may constitute a new subgroup in this family of enzymes', American Journal of Molecular Biology, vol. 4, no. 1, pp. 11-15. Daumann, L, Larrabee, J, Ollis, D et al 2014, 'Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation', Journal of Inorganic Biochemistry, vol. 131, pp. 1-7. Daumann, L, Schenk, G, Ollis, D et al 2014, 'Spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes', Dalton Transactions, vol. 43, no. 3, pp. 910-928. Jackson, C, Carville, A, Ward, J et al 2014, 'Use of OpdA, an organophosphorus (OP) hydrolase, prevents lethality in an African green monkey model of acute OP poisoning', Toxicology, vol. 317, pp. 1-5. Pedroso, M, Ely, F, Lonhienne, T et al 2014, 'Determination of the catalytic activity of binuclear metallohydrolases using isothermal titration calorimetry', Journal of Biological Inorganic Chemistry, vol. 19, no. 3, pp. 389-398. Porter, J, Carr, P, Collyer, C et al 2014, 'Crystallization of dienelactone hydrolase in two space groups: structural changes caused by crystal packing', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 70, no. 7, pp. 884-889. Ma, P, Kanizaj, N, Chan, S et al 2014, 'The Escherichia coli glucuronylsynthase promoted synthesis of steroid glucuronides: improved practicality and broader scope', Organic and Biomolecular Chemistry, vol. 12, no. 32, pp. 6208-6214. Carr, P, Ewens, C, Dai, J et al 2014, 'Crystal structure of the mouse interleukin-3 ?2-receptor: insights into interleukin-3 binding and receptor activation', Biochemical Journal, vol. 463, no. 3, pp. 393-403. Phelan, E, Miraula, M, Selleck, C et al 2014, 'Metallo-?2-lactamases: a major threat to human health', American Journal of Molecular Biology, vol. 4, no. 3, pp. 89-104. Pedroso, M, Ely, F, Mitic, N et al 2014, 'Comparative investigation of the reaction mechanisms of the organophosphate-degrading phosphotriesterases from Agrobacterium radiobacter (OpdA) and Pseudomonas diminuta (OPH)', Journal of Biological Inorganic Chemistry, vol. 19, no. 8, pp. 1263-1275. Daumann, L, McCarthy, B, Hadler, K et al 2013, 'Promiscuity comes at a price: Catalytic versatility vs efficiency in different metal ion derivatives of the potential bioremediator GpdQ', Biochimica et Biophysica Acta: Proteins & Proteomics, vol. 1834, no. 1, pp. 425-432. Liu, J & Ollis, D 2013, 'Screening libraries for improved solubility: using E. coli dihydrofolate reductase as a reporter', Methods in Molecular Biology, vol. 978, pp. 229-236. Stevenson, B, Yip, S & Ollis, D 2013, 'In vitro directed evolution of enzymes expressed by E. coli in microtiter plates', Methods in Molecular Biology, vol. 978, pp. 237-249.