研究领域

Cellular Nervous System

Dr Moorhouse's broad interest are in the molecular and cellular basis of neuronal excitability, focussing on how membrane transporters and ion channels contribute to electrical and chemical signalling in the brain. He leads the Neurobiology Research Lab that aims to develop improved and novel strategies to treat different brain disorders by gaininmg greater understanding of underlying cellular mechanisms. In collaboration with colleagues at UNSW and beyond, we use a range of techniques, including electrophysiology, cell culture and mutagenesis, live cell imaging, biochemical and histological approaches and behavioural analysis. Dr Moorhouse and his colleagues have published over 40 journal articles in this area with over 1300 citations. Dr Moorhouse's current research focus is on; i) the role of KCC2 transporter in Cl- homeostasis and neuronal inhibition in health and disease; ii) the molecular basis of ion permeation in GABA and glycine receptors, and iii) novel flavonoid modulators of glycine receptors.

近期论文

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Miyamoto A; Wake H; Ishikawa AW; Eto K; Shibata K; Murakoshi H; Koizumi S; Moorhouse AJ; Yoshimura Y; Nabekura J, 2016, 'Microglia contact induces synapse formation in developing somatosensory cortex.', Nature Communications, vol. 7, pp. 12540, http://dx.doi.org/10.1038/ncomms12540 Kim SK; Hayashi H; Ishikawa T; Shibata K; Shigetomi E; Shinozaki Y; Inada H; Roh SE; Kim SJ; Lee G; Bae H; Moorhouse AJ; Mikoshiba K; Fukazawa Y; Koizumi S; Nabekura J, 2016, 'Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain.', Journal of Clinical Investigation, vol. 126, no. 5, pp. 1983 - 1997, http://dx.doi.org/10.1172/JCI82859 Kato G; Inada H; Wake H; Akiyoshi R; Miyamoto A; Eto K; Ishikawa T; Moorhouse AJ; Strassman AM; Nabekura J, 2016, 'Microglial Contact Prevents Excess Depolarization and Rescues Neurons from Excitotoxicity.', eNeuro, vol. 3, no. 3, http://dx.doi.org/10.1523/ENEURO.0004-16.2016 Alexander SPH; Benson HE; Faccenda E; Pawson AJ; Sharman JL; McGrath JC; Catterall WA; Spedding M; Peters JA; Harmar AJ, 2013, 'The Concise Guide to PHARMACOLOGY 2013/14: Overview', British Journal of Pharmacology, vol. 170, no. 8, pp. 1449 - 1458, http://dx.doi.org/10.1111/bph.12444 Miyamoto A; Wake H; Moorhouse AJ; Nabekura J, 2013, 'Microglia and synapse interactions: Fine tuning neural circuits and candidate molecules', Frontiers in Cellular Neuroscience, vol. 7, no. MAY, http://dx.doi.org/10.3389/fncel.2013.00070 Barry PH; Lewis TM; Moorhouse AJ, 2013, 'An optimised 3 M KCl salt-bridge technique used to measure and validate theoretical liquid junction potential values in patch-clamping and electrophysiology', European Biophysics Journal, vol. 42, no. 8, pp. 631 - 646, http://dx.doi.org/10.1007/s00249-013-0911-3 Wake H; Moorhouse AJ; Miyamoto A; Nabekura J, 2013, 'Microglia: actively surveying and shaping neuronal circuit structure and function', Trends in Neurosciences, vol. 36, no. 4, pp. 209 - 217, http://dx.doi.org/10.1016/j.tins.2012.11.007 Wake H; Moorhouse AJ; Nabekura J, 2012, 'Functions of microglia in the central nervous system-beyond the immune response', Neuron Glia Biology, vol. 7, no. 1, pp. 47 - 53, http://dx.doi.org/10.1017/S1740925X12000063 Eto K; Ishibashi H; Yoshimura T; Watanabe M; Miyamoto A; Ikenaka K; Moorhouse AJ; Nabekura J, 2012, 'Enhanced GABAergic activity in the mouse primary somatosensory cortex is insufficient to alleviate chronic pain behavior with reduced expression of neuronal potassium-chloride cotransporter', The Journal of Neuroscience, vol. 32, no. 47, pp. 16552 - 16559, http://dx.doi.org/10.1523/JNEUROSCI.2104-12.2012 Kim Y; Wong ACY; Power JM; Tadros SF; Klugmann M; Moorhouse AJ; Bertrand PP; Housley GD, 2012, 'Alternative splicing of the TRPC3 ion channel calmodulin/ IP 3 receptor-binding domain in the hindbrain Enhances cation flux', The Journal of Neuroscience, vol. 32, no. 33, pp. 11414 - 11423, http://dx.doi.org/10.1523/JNEUROSCI.6446-11.2012 Barry PH; Sugiharto S; Lewis TM; Moorhouse AJ, 2010, 'Further analysis of counterion permeation through anion-selective glycine receptor channels', Channels, vol. 4, no. 3 Sugiharto S; Carland JE; Lewis TM; Moorhouse AJ; Barry PH, 2010, 'External divalent cations increase anion-cation permeability ratio in glycine receptor channels', Pfluegers Archiv: European journal of physiology, vol. 460, no. 1, pp. 131 - 152, http://dx.doi.org/10.1007/s00424-010-0792-6 Nakahata Y; Miyamoto A; Watanabe M; Moorhouse AJ; Nabekura J; Ishibashi H, 2010, 'Depolarizing shift in the GABA-induced current reversal potential by lidocaine hydrochloride', Brain Research, vol. 1345, pp. 19 - 27, http://dx.doi.org/10.1016/j.brainres.2010.05.052 Wake H; Moorhouse AJ; Jinno S; Kohsaka S; Nabekura J, 2009, 'Resting microglia directly monitor the functional state of synapses in vivo and determine the fate of ischemic terminals', The Journal of Neuroscience, vol. 29, no. 13, pp. 3974 - 3980, http://dx.doi.org/10.1523/JNEUROSCI.4363-08.2009 Carland JE; Cooper MA; Sugiharto S; Jeong HJ; Lewis TM; Barry PH; Peters JA; Lambert JJ; Moorhouse AJ, 2009, 'Characterization of the effects of charged residues in the intracellular loop on ion permeation in α1 glycine receptor channels', Journal of Biological Chemistry, vol. 284, no. 4, pp. 2023 - 2030, http://dx.doi.org/10.1074/jbc.M806618200 Watanabe M; Wake H; Moorhouse AJ; Nabekura J, 2009, 'Clustering of neuronal K+-Cl- cotransporters in lipid rafts by tyrosine phosphorylation', Journal of Biological Chemistry, vol. 284, no. 41, pp. 27980 - 27988, http://dx.doi.org/10.1074/jbc.M109.043620 Sugiharto S; Lewis TM; Moorhouse AJ; Schofield PR; Barry PH, 2008, 'Anion-cation permeability correlates with hydrated counterion size in glycine receptor channels', Biophysical Journal, vol. 95, no. 10, pp. 4698 - 4715, http://dx.doi.org/10.1529/biophysj.107.125690 Park EJ; Choi IS; Cho JH; Nakamura M; Lee JJ; Lee MG; Choi BJ; Moorhouse AJ; Jang IS, 2008, 'Zinc modulation of glycine receptors in acutely isolated rat CA3 neurons', Life Sciences, vol. 83, no. 5-6, pp. 149 - 154, http://dx.doi.org/10.1016/j.lfs.2008.04.024 Wake H; Watanabe M; Moorhouse AJ; Kanematsu T; Horibe S; Matsukawa N; Asai K; Ojika K; Hirata M; Nabekura J, 2007, 'Early changes in KCC2 phosphorylation in response to neuronal stress result in functional downregulation', The Journal of Neuroscience, vol. 27, no. 7, pp. 1642 - 1650, http://dx.doi.org/10.1523/JNEUROSCI.3104-06.2007 Qu W; Moorhouse AJ; Chandra M; Pierce KD; Lewis TM; Barry PH, 2006, 'A single P-loop glutamate point mutation to either lysine or arginine switches the cation-anion selectivity of the CNGA2 channel', Journal of General Physiology, vol. 127, no. 4, pp. 375 - 389, http://dx.doi.org/10.1085/jgp.200509378