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个人简介

教育经历 2003.7. 西安交通大学 理学学士 应用物理学 2006.5 西安交通大学 理学硕士 理论物理(斑图动力学) 2010.6 西安交通大学 理学博士 凝聚态物理(电子显微镜学) 2008-2010 美国加州大学旧金山分校 联合培养博士 蛋白结构与功能(电子显微镜) 2011-2014 美国劳伦斯伯克利国家实验室 博士后 蛋白结构与功能(电子显微镜) 工作经历 2011.1–2014.1 博士后研究员 美国劳伦斯伯克利国家实验室材料科学部 2014.2–2015.9 项目科学家 美国劳伦斯伯克利国家实验室材料科学部 2015.9–今 物理学教授 西安交通大学理学院应用物理系 其他经历和专业资格 2006-2008 中国物理学会学生会员 2009-2010 美国生物物理学会学生会员 2010-今 美国生物物理学会会员 2012-今 美国心脏病学会会员 2015-今 中国物理学会终身会员 2016-今 中国生物物理学会冷冻电子显微学分会 理事 2016-今 中国细胞生物学学会细胞工程与转基因生物分会 常务委员 荣誉和奖励 2005 西安交通大学优秀研究生 2005 西安交通大学理学院院级成绩第一,获“和舰科技”奖学金 2009 西安交通大学优秀毕业生 2009 西安交通大学优秀研究生干部 2011 陕西省优秀博士论文 2015 入选西安交通大学“青年拔尖人才A类计划”

研究领域

1继续开发和应用多种透射电子显微镜技术,揭示了运载人体胆固醇的胆固醇酯转移蛋白的结构与机理,对脂蛋白体系进行深入的结构-功能研究,对人类心血管疾病类药物的研发提供关键支持。 心血管疾病是人类健康的第一杀手,而人体胆固醇含量过高是导致心血管疾病的最重要因素之一。作为胆固醇运输的主要蛋白质,高/低密度脂蛋白和胆固醇酯转移蛋白是其中的关键生物大分子。由于脂蛋白的结构活性非常强,目前透射电子显微镜是最佳的结构探测工具;更关键的是,透射电镜可以直观地观察到蛋白质之间的相互作用形式,为其结合机理方面的研究提供了关键数据。 借助课题组开发的优化负染色方法和冷冻正染色技术,我们首次应用透射电镜研究了胆固醇酯转移蛋白与脂蛋白的结合形态,以及各种脂蛋白的结构,相关结果发表在Nature Chemical Biology, Structure, Scientific Reports, Journal of Lipid Research等高水平学术期刊上。未来我们将继续这一研究路线,继续深入研究脂蛋白家族的结构与功能机理。 2继续开发和应用透射电子显微镜的电子断层成像技术,完善Individual-particle electron tomography方法,研究单个软物质大分子的空间结构和动态变化。 一般而言,蛋白质等软物质类大分子结构测定的主要手段包括X光晶体衍射法,核磁共振谱法,和传统的单颗粒电镜重构等方法。这些方法往往会受限于大分子是否能够结晶、分子量大小或结构是否单一等约束条件,所得到的一般是在某种特定条件下的大分子的固定态,往往无法获得任意单个蛋白质个体大分子的三维结构,从而无法捕捉到其动态变化过程和大分子之间的个体结构差异,影响对与之密切相关的功能的理解。 发展和利用多种优化的电子显微断层成像技术并有机结合独立开发的三维重构算法,我们发展了这套测定任意单个蛋白质个体分子的三维结构方法,并命名为“Individual-Particle Electron Tomography (IPET)”,将单物体电子断层成像技术的应用范围从以往的细胞尺度(微米)推进到了纳米尺度。近期,应用此方法,我们科研小组系统完成了对人体抗体及其药物复合物的动态结构的电子显微镜三维结构研究,以及双螺旋DNA空间动态结构的研究,所得结果发表在Nature Communications和Scientific Reports等高水平刊物上。 3综合运用各种冷冻透射电镜技术,研究具有重大意义的软物质大分子(包括蛋白质、有机高分子、有机无机复合物等)的结构和机理。

近期论文

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1. Minggang Xia, Shengli Zhang, Xianjun Zuo, Erhu Zhang, Shumin Zhao, Jian Li, Lei Zhang, Yachao Liu, and Run Liang, Assignment of the chiralities of double-walled carbon nanotubes using two radial breathing modes, Phys. Rev. B 70(20), 205428 (2004). (IF: 3.718;) 2. Shengli Zhang, Lei Zhang, Run Liang, Erhu Zhang, Yachao Liu, and Shumin Zhao, Lubricating bacteria model for the growth of bacterial colonies exposed to ultraviolet radiation, Phys. Rev. E 72(5), 051913 (2005). (IF: 2.252) 3. Shengli Zhang, Run Liang, Erhu Zhang, Lei Zhang, and Yachao Liu, Magnetosubbands of semiconductor quantum wires with Rashba and Dresselhaus spin-orbit coupling, Phys. Rev. B 73(15), 155316 (2006). (IF: 3.718) 4. Shumin Zhao, Shengli Zhang, Zhenwei Yao, and Lei Zhang, Equilibrium conformation of polymer chains with noncircular cross section, Phys. Rev. E74(3), 032801 (2006). (IF: 2.252) 5. Youli Yu, Lei Zhang, and Zhenxi Zhang, Computation for Optical Trap Stiffness Versus Refractive Index of Biological Cell, Acta. Optica. Sinica. 27(6), 1076 (2007). 6. Shengli Zhang, Yachao Liu, Minggang Xia, Lei Zhang, Erhu Zhang, Run Liang, and Shumin Zhao, Long-wavelength optical phonons in single-walled boron nitride nanotubes, Physica B 403(23-24), 4196-4201 (2008). (IF: 1.352) 7. Minggang Xia, Lei Zhang, and Shengli Zhang, Effect of optical phonons scattering>Physics Lett. A 373(3), 385-390 (2009). (IF: 1.677) 8. Lei Zhang, Shengli Zhang, Huimin Tong, Dongsheng Lei, and Bambi Hu, Spontaneously periodic wave generation in coupled excitable media, Phys.Rev. E 79(5), 056213 (2009). (IF: 2.252) 9. Lei Zhang, James Song, Yvonne Newhouse, Shengli Zhang, Karl H. Weisgraber, and Gang Ren, An optimized negative-staining protocol of electron microscopy for apoE4•POPC lipoprotein, Journal. Lipid Research 51(5), 1228-1236 (2010). (IF: 4.368) 10. Martin K. Jones, Lei Zhang, Andrea Catte, Ling Li, Michael N .Oda, Gang Ren, and Jere P. Segrest, Assessment of the Validity of the Double Superhelix Model for Reconstituted High Density Lipoproteins: A COMBINED COMPUTATIONAL-EXPERIMENTAL APPROACH, Journal. Biological Chemistry 285(52), 41161-41171 (2010). (IF: 4.258) 11. Won Jin Cho, Jin-Sook Lee, Lei Zhang, Gang Ren, Leah Shin, Charles W. Manke, Jeffrey Potoff, Nato Kotaria, Mzia G. Zhvania, Bhanu P. Jena, Membrane-directed molecular assembly of the neuronal SNARE complex, Journal. Cellular and Molecular Medicine, 15(1), 31-37 (2011). (IF: 4.938) 12. Lei Zhang, James Song, Giorgio Cavigiolio, Brian Y.Ishida, Shengli Zhang, Karl H. Weisgraber, Henry J. Pownall, Kerry-Anne Rye, John P. Kane, Michael N.Oda, and Gang Ren, Morphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy, Journal. Lipid Research 52(1), 175-184 (2011). (IF: 4.368) 13. Xiaobo Zhai, Shengli Zhang, Lei Zhang, and Shumin Zhao, The optimal configuration of gel sheet governed by its concentration, Int. J. Mod. Phys. B 26(14): 1250084 (2012). (IF: 0.851) 14. Lei Zhang and Gang Ren, IPET and FETR: Experimental Approach for Studying Molecular Structure Dynamics by Cryo-Electron Tomography of a Single-Molecule Structure, PLOS ONE, 7(1): e30249 (2012). (IF: 3.057) 15. Lei Zhang, Feng Yan, Shengli Zhang, Dongsheng Lei, M. Arthur Charles, Giorgio Cavigiolio, Michael Oda, Ronald M. Krauss, Karl H. Weisgraber, Kerry-Anne Rye, Henry J. Pownall, Xiayang Qiu, and Gang Ren. Structural basis of transfer between lipoproteins by cholesteryl ester transfer protein,Nature Chemical Biology, 8(4): 342-349 (2012). (IF:12.709) 16. Lei Zhang and Gang Ren, High-resolution single-molecule structure revealed by electron microscopy and individual-particle electron tomography, J. Phys. Chem. Biophys, 2(2): DOI: 10.4172/2161-0398.2000e103 1000e103 (2012). 17. Huimin Tong, Lei Zhang, Liqing Huang, and Gang Ren, Optimized Negative-staining Protocol for Electron Microscopy Study of Lipoprotein Structure, Progress in Biochemistry and Biophysics, 39(10): 972-978 (2012). (IF:0.224) 18. Lei Zhang, Huimin Tong, Mark Garewal, and Gang Ren, Optimized Negative-Staining Electron Microscopy for Lipoprotein studies, BBA-General Subjects 1830(1): 2150-2159 (2013). (IF:5.083) 19. Dongsheng Lei, Xing Zhang, Shengbo Jiang, Zhaodi Cai, Matthew J. Rames, Lei Zhang, Gang Ren, and Shengli Zhang, Structural features of cholesteryl ester transfer protein: A molecular dynamics simulation study,Proteins: Structure, Function, and Bioinformatics 81(3): 415-425 (2013). (IF:2.499) 20. Huimin Tong, Lei Zhang, Allan Kaspar, Matthew J. Rames, Liqing Huang, Gary Woodnutt, and Gang Ren, Peptide-Conjugation Induced Conformational Changes in Human IgG1 Observed by Optimized Negative-Staining and Individual-Particle Electron Tomography, Scientific Reports, 3: 1089 (2013). (IF:5.228) 21. Teng Zhang, Yunhui Peng, Huimin Tong, Matthew J. Rames, Lei Zhang, Gang Ren, IPET: an Experimental Method to Determine the 3-Dimensional Structure of an Individual Macromolecule, Progress in Chemistry, 25(05): 669-676 (2013). (IF:0.894) 22. Kang-Da Zhang, Jia Tian, David Hanifi, Yuebiao Zhang, Andrew Chi-Hau Sue, Tian-You Zhou, Lei Zhang, Xin Zhao, Yi Liu, and Zhan-Ting Li. Toward a Single-Layer Two-Dimensional Honeycomb Supramolecular Organic Framework in Water, Journal of The American Chemical Society,135(47): 17913-17918 (2013). (IF:13.038) 23. Dongsheng Lei, Huimin Tong, Lei Zhang, Xing Zhang, Shengli Zhang, and Gang Ren, Structure and Function of Cholesteryl Ester Transfer Protein in Transferring Cholesteryl Ester, Progress in Chemistry, 26(5):879-888 (2014). (IF:0.894) 24. Debin Wang, Stacy L. Capehart, Suchetan Pal, Minghui Liu, Lei Zhang, P. James Schuck, Yan Liu, Hao Yan, Matthew B. Francis, and James J. De Yoreo, Hierarchical Assembly of Plasmonic Nanostructures Using Virus Capsid Scaffolds>ACS Nano, 8(8):7896-7904 (2014). (IF:13.334) 25. Zhuoyang Lu, Yun Wang, Fang Chen, Huimin Tong, M.V.V.V. Sekhar Reddy, Lin Luo, Suchithra Seshadrinathan, Lei Zhang, Luis Marcelo F. Holthauzen, Ann Marie Craig, Gang Ren. and Gabby Rudenko, Calsyntenin3: Molecular Architecture and Interaction with Neurexin 1alpha, J. Biological Chemistry: 289(50):34530-34542 (2014). (IF:4.258) 26.Meng Zhang, River Charles, Huimin Tong, Lei Zhang, Mili Patel, Francis Wang, Matthew J. Rames, Amy Ren, Kerry-Anne Rye, Xiayang Qiu, DouglasG. Johns, M.Arthur Charles, and Gang Ren. HDL surface lipids mediate CETP binding as revealed by electron microscopy and molecular dynamics simulation. Scientific Reports, 5:8741 (2015). (IF:5.228) 27. Lei Zhang, Xing Zhang, Huimin Tong, Bo Peng, Matthew J. Rames, Shengli Zhang, and Gang Ren. 3D structural fluctuation of IgG1 antibody revealed by individual particle electron tomography. Scientific Reports, 5: 9803 (2015). (IF:5.228) 28. Xing Zhang, Donsheng Lei, Lei Zhang, Matthew Rames, and Shengli Zhang. A Model of Lipid-Free Apolipoprotein A-I Revealed by Iterative Molecular Dynamics Simulation. PLOS ONE, 10(3): e0120233 (2015). (IF:3.057) 29. Jere P. Segrest, Martin K. Jones, Andrea Catte, Medha Manchekar, Geeta Datta, Robin Zhang, Lei Zhang, Ling Li, James C. Patterson, Mayakonda N. Palgunachari, Jack F. Oram, and Gang Ren, Surface Density-Induced Pleating of a Lipid Monolayer Drives Nascent High-Density Lipoprotein Assembly. Structure, 23(7): 1214-1226 (2015). (IF:5.237) 30.Lei Zhang, Dongsheng Lei, Jessica M. Smith, Meng Zhang, Huimin Tong, Xing Zhang, Zhuoyang Lu, Jiankang Liu, Paul A. Alivisatos, and Gang Ren, Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography. Nature Communications, 7:11083 (2016). (IF:11.329) 31. Zhiwei Yang, Fei Wu, Xiaohui Yuan, Lei Zhang, and Shengli Zhang, Novel binding patterns between ganoderic acids and neuraminidase: Insights from docking, molecular dynamics and MM/PBSA studies. Journal of Molecular Graphics & Modelling 65: 27-34 (2016). (IF:1.674) 32. Dongsheng Lei, Mattehw Rames, Xing Zhang,Lei Zhang, Shengli Zhang, and Gang Ren, Insights into the tunnel mechanism of cholesteryl ester transfer protein through all-atom molecular dynamics simulations. J. Biological Chemistry, 291(27):14034-14044 (2016). (IF:4.258) 33. Xing Zhang, Yuheng Liao, Huimin Tong, Lei Zhang, Shengli Zhang, Gang Ren, 3D Reconstruction and Structural Study of IgG1 Antibody by Individual-particle Electron Tomography. Progress in Biochemistry and Biophysics 43(9): 839-849 (2016). (IF:0.224) 34. Zhuoyang Lu, M. V. V. V. Sekhar Reddy, Jianfang Liu, Ana Kalichava, Jiankang Liu, Lei Zhang, Fang Chen, Yun Wang, Luis Marcelo F. Holthauzen, Mark A. White, Suchithra Seshadrinathan, Xiaoying Zhong, Gang Ren, and Gabby Rudenko, Molecular architecture of contactin-associated protein-like 2 (cntnap2) and its interaction with contactin 2 (cntn2). J. Biological Chemistry, 291(46): 24133-24147 (2016). (IF:4.258) 35. Mingxing Wang, Guoyin Li, Zhiwei Yang, Lei Wang, Lei Zhang, Ting Wang, Yimeng Zhang, Shengli Zhang, Yong Han, and Lintao Jia, Uncoupling protein 2 downregulation by hypoxia through repression of peroxisome proliferator-activated receptor γ promotes chemoresistance of non-small cell lung cancer. Oncotarget 2016. 36. Zhiwei Yang, Lu Gou, Shuyu Chen, Na Li, Shengli Zhang*, and Lei Zhang*, Membrane Fusion involved in Neurotransmission: Glimpse from Electron Microscope and Molecular Simulation. Frontiers in Molecular Neuroscience, 10:168, 2017. 37. Dongsheng Li*, Jaehun Chun*, Dongdong Xiao, Weijiang Zhou, Huacheng Cai, Lei Zhang, Kevin M. Rosso, Christopher J. Mundy, Gregory K. Schenter, and James J. De Yoreo, Trends in mica-mica adhesion reflect the influence of molecular details>PNAS, 114(29): 7537-7542 (2017). 38. Zhiwei Yang, Yang Cao, Dongxiao Hao, Xiaohui Yuan, Lei Zhang, and Shengli Zhang*, Binding profiles of cholesterol ester transfer protein with current inhibitors: a look at mechanism and drawback. Journal of Biomolecular Structure and Dynamics, 36: 2567-2580, 2018. 39. Meng Zhang, Dongsheng Lei, Bo Peng, Mickey Yang, Lei Zhang, M. Art Charles, Kerry-Anne Rye, Ronald M. Krauss, Douglas G. Johns, Gang Ren*, Assessing the mechanisms of cholesteryl ester transfer protein inhibitors. BBA - Molecular and Cell Biology of Lipids, 1862 (12): 1606, 2018. 40. Lu Gou, Taoli Jin, Shuyu Chen, Na Li, Dongxiao Hao, Shengli Zhang, and Lei Zhang*, Bio-macromolecular dynamic structures and functions, illustrated with DNA, antibody, and lipoprotein. Chinese Physics B, 27(2):028708, 2018. 41. Shuyu Chen#, Na Li#, Taoli Jin, Lu Gou, Dongxiao Hao, Zhiqi Tian, Shengli Zhang, Lei Zhang*, Lipoprotein in cholesterol transport: Highlights and recent insights into its structural basis and functional mechanism, Chinese Physics B, 27(2):028702, 2018. 42. Zhiwei Yang#, Dongxiao Hao#, Yizhuo Che, Jiahui Yang, Lei Zhang*, and Shenlgi Zhang*, Mutation-induced spatial differences in neuraminidase structure and sensitivity to neuraminidase inhibitors, Chinese Physics B, 27(1):018704, 2018. 43. Dongxiao Hao, Zhiwei Yang, Teng Gao, Zhiqi Tian, Lei Zhang*, and Shengli Zhang*, Role of glycans in cholesteryl ester transfer protein revealed by molecular dynamics simulation, Proteins 86:882-891, 2018. 44. Zhiqi Tian, Michael Crowe, Liqin Shao, Peng Zhang, Lei Zhang*, Qing Zhao*, and Jiajie Diao*, Interface Colloidal Deposition of Nanoparticle Wire Structures, Particle & Particle Systems Characterization, 35:1800098, 2018. 45. QianChun Wang, XiaoBo Zhai, Michael Crowe, Lu Gou, YinFeng Li, DeChang Li*, Lei Zhang*, JiaJie Diao*, and BaoHua Ji*, Heterogeneous Oxidization of Graphene Nanosheets Damages Membrane, Science China Physics, Mechanics & Astronomy, 62(6): 064611, 2019. 46. Hongtao Ren#, Zixin Xiong#, Enze Wang#, Zhiquan Yuan, Yufei Sun, Kunlei Zhu, Bolun Wang, Xuewen Wang, Hanyuan Ding, Peng Liu, Lei Zhang*, Junqiao Wu, Shoushan Fan, Xiaoyan Li*, and Kai Liu*, Watching Dynamic Self-assembly of Web Buckles in Strained MoS2 Thin Films, ACS Nano, 13:3106-3116, 2019 47. Baojun Lin, Lin Zhang, Heng Zhao, Xianbin Xu, Ke Zhou, Song Zhang, Lu Gou, Baobing Fan, Lei Zhang, Hongping Yan, Xiao Dan Gu*, Lei Ying, Fei Huang*, Yong Cao, Wei Ma*, Molecular packing control enables excellent performance and mechanical property of blade-cast all-polymer solar cells, Nano Energy, 59:277-284, 2019 48. Hongtao Ren, Gang Xiang*, Jiating Lu, Xi Zhang, Lei Zhang*, Biaxial Strain-Mediated Room Temperature Ferromagnetism of ReS2 Web Buckles, Advanced Electronic Materials, 1900814, 2019 49. Boyu Liu#, Fei Liu#, Nan Yang, Xiaobo Zhai, Lei Zhang, Yang Yang, Bin Li, Ju Li, Evan Ma, Jianfeng Nie, Zhiwei Shan*, Large plasticity in magnesium mediated by pyramidal dislocations, Science, 365:73-75, 2019 50. Xiaoman Ma, Fang Pan, Haoqi Li, Peng Shen, Chao Ma, Lei Zhang, Haibo Niu, Youzhang Zhu, Honggang Ye*, Mechanism of Single-Photon Upconversion Photoluminescence in All-Inorganic Perovskite Nanocrystals: The Role of Self-Trapped Excitons, The Journal of Physical Chemistry Letters, 10:5989-5996, 2019 51. Ke Sun#, Changjian Zhao#, Yuejia Chen#, Xiaojun Zeng#, Xin Jiang#, Xianting Ding, Lu Gou, Haiyang Xie, Xinqiong Li, Xialin Zhang, Sheng Lin, Linqin Dou, Long Wei, Haofu Niu, Ming Zhang, Ruocen Tian, Erica Sawyer, Qingyue Yuan, Yuqin Huang, Piaopiao Chen, Chengjian Zhao, Cuisong Zhou, Binwu Ying, Bingyang Shi, Xiawei Wei, Ruotian Jiang, Lei Zhang*, Guangwen Lu*, Jia Geng*, Active DNA Unwinding and Transport by a Membrane-Adapted Helicase Nanopore, Nature Communications, 10:5083, 2019 52. Haohui Huo, Tianyi Tan, Lu Gou, Long Chen, Lei Zhang, Qilu Zhang*, Feng Liu*, Single-chain tethered nanoparticles with tunable softness: scalable synthesis and unique self-assembly behavior, Polymer Chemistry, 10:6183, 2019. 53. Zhen Li, Shuyu Chen, Chendi Gao, Zhiwei Yang, Kuo-Chih Shih, Zdravko Kochovski, Guang Yang, Lu Gou, Mu-Ping Nieh, Ming Jiang, Lei Zhang*, Guosong Chen*, Chemically Controlled Helical Polymorphism in Protein Tubes by Selective Modulation of Supramolecular Interactions, Journal of the American Chemical Society, 141: 19448-19457, 2019. 54. Hongtao Ren, Yachao Liu, Lei Zhang*, Kai Liu*. Synthesis, properties, and applications of large-scale two-dimensional materials by polymer-assisted deposition, Journal of Semiconductors, 40: 061003, 2019. 55. Hanyuan Ding, Zeqin Xin, Yueyang Yang, Yufeng Luo, Kailun Xia, Bolun Wang, Yufei Sun, Jiaping Wang, Yingying Zhang, Hui Wu, Shoushan Fan, Lei Zhang*, Kai Liu*, Ultrasensitive, Low-Voltage Operational, and Asymmetric Ionic Sensing Hydrogel for Multipurpose Applications, Advanced Functional Materials, 201909616, 2020. 56. Hongtao Ren, Lei Zhang*, Gang Xiang*, Web buckle-mediated room-temperature ferromagnetism in strained MoS2 thin films, Applied Physics Letters, 116, 012401, 2020.

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