个人简介

B.S. Biology/Chemistry, B.A. Philosophy/Theology, Point Loma Nazarene University, 2004 Ph.D. Chemistry, University of California, Berkeley, 2009 (Christopher J. Chang, advisor) Post-doctoral Fellow, University of California, San Diego, 2009-2013 (Roger Y. Tsien, advisor) NIH NRSA Post-doctoral fellow, NIBIB, 2010 NIH K99 Pathway to Independence Fellow, NINDS, 2012 Assistant Professor, Chemistry and Molecular & Cell Biology, University of California, Berkeley, 2013

研究领域

Chemical biology, organic chemistry, fluorescence microscopy, neuroscience, imaging. Research in the Miller lab operates at the interface of synthetic chemistry, biology, and neuroscience. We seek to exploit expertise in synthetic chemistry, probe design, imaging, molecular biology, and electrophysiology to create and deploy molecular tools for studying the nervous system. This multi-faceted approach engages a diverse group of researchers from differing scientific backgrounds, expanding our understanding of basic chemical and biological processes, and using these discoveries to investigate the brain. Specifically, we seek to address how the brain transmits information from cell to cell and develop tools to track neuronal activity with high spatial and temporal resolution. This approach to studying neuronal communication and information flux in the brain is two-pronged. First, we will develop activity-dependent neuronal tracer dyes for following signal transduction through neural circuits and within cells. Secondly, we will investigate new synthetic and genetically encoded indicators for optically monitoring voltage changes in neurons. Throughout, these approaches will be integrated into systems ranging from primary cell culture to tissue slices to whole animals in order to not only show-case the utility of our new tools, but also to explore new dimensions of neuronal communication and information transfer.

Chemical biology, organic chemistry, fluorescence microscopy, neuroscience, imaging. Research in the Miller lab operates at the interface of synthetic chemistry, biology, and neuroscience. We seek to exploit expertise in synthetic chemistry, probe design, imaging, molecular biology, and electrophysiology to create and deploy molecular tools for studying the nervous system. This multi-faceted approach engages a diverse group of researchers from differing scientific backgrounds, expanding our understanding of basic chemical and biological processes, and using these discoveries to investigate the brain. Specifically, we seek to address how the brain transmits information from cell to cell and develop tools to track neuronal activity with high spatial and temporal resolution. This approach to studying neuronal communication and information flux in the brain is two-pronged. First, we will develop activity-dependent neuronal tracer dyes for following signal transduction through neural circuits and within cells. Secondly, we will investigate new synthetic and genetically encoded indicators for optically monitoring voltage changes in neurons. Throughout, these approaches will be integrated into systems ranging from primary cell culture to tissue slices to whole animals in order to not only show-case the utility of our new tools, but also to explore new dimensions of neuronal communication and information transfer.

近期论文

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Huang, Y. L.; Walker, A. S.; Miller, E. W., A Photostable Silicon Rhodamine Platform for Optical Voltage Sensing. J Am Chem Soc 2015, "just accepted" DOI: 10.1021/jacs.5b06644. [pdf] [html] Miller, E. W., Chemical biologists rush to San Francisco for the ICBS. Nat Chem Biol 2015, 11, 91. [pdf] [html] Rubinstein, N.; Liu, P.; Miller, E. W.; Weinstain, R., meso-Methylhydroxy BODIPY: a scaffold for photo-labile protecting groups. Chem Commun 2015, 51, 6369. [pdf] [html] Woodford, C. R.; Frady, E. P.; Smith, R. S.; Morey, B.; Canzi, G.; Palida, S. F.; Araneda, R. C.; Kristan, W. B., Jr.; Kubiak, C. P.; *Miller, E. W.; *Tsien, R. Y., Improved PeT Molecules for Optically Sensing Voltage in Neurons. J Am Chem Soc 2015, 137, 1817. [pdf] [html] Dolensek, J.; Stozer, A.; Skelin Klemen, M.; Miller, E. W.; Slak Rupnik, M., The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices. PLoS One 2013, 8, e82374. [pdf] [html] Moshtagh-Khorasani, M.; Miller, E. W.; Torre, V., The spontaneous electrical activity of neurons in leech ganglia. Physiol Rep 2013, 1, e00089. [pdf] [html] Miller, E. W.; Lin, J. Y.; Frady, E. P.; Steinbach, P. A.; Kristan, W. B.; Tsien, R. Y., Optically monitoring voltage in neurons by photo-induced electron transfer through molecular wires. Proc Natl Acad Sci U S A 2012, 109 (6), 2114-2119. [pdf] [html] Dodani, S. C.; Domaille, D. W.; Nam, C. I.; Miller, E. W.; Finney, L. A.; Vogt, S.; Chang, C. J., Calcium-dependent copper redistributions in neuronal cells revealed by a fluorescent copper sensor and X-ray fluorescence microscopy. Proc Natl Acad Sci U S A 2011, 108 (15), 5980-5. [pdf] [html] Miller, E. W.; Taulet, N.; Onak, C. S.; New, E. J.; Lanselle, J. K.; Smelick, G. S.; Chang, C. J., Light-Activated Regulation of Cofilin Dynamics Using a Photocaged Hydrogen Peroxide Generator. J Am Chem Soc 2010. [pdf] [html] Miller, E. W.; Dickinson, B. C.; Chang, C. J., Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling. Proc Natl Acad Sci U S A 2010, 107 (36), 15681-6. [pdf] [html] Cheng, W. Y.; Tong, H.; Miller, E. W.; Chang, C. J.; Remington, J.; Zucker, R. M.; Bromberg, P. A.; Samet, J. M.; Hofer, T. P., An integrated imaging approach to the study of oxidative stress generation by mitochondrial dysfunction in living cells. Environ Health Perspect 2010, 118 (7), 902-8. [pdf] Bao, L.; Avshalumov, M. V.; Patel, J. C.; Lee, C. R.; Miller, E. W.; Chang, C. J.; Rice, M. E., Mitochondria are the source of hydrogen peroxide for dynamic brain-cell signaling. J Neurosci 2009, 29 (28), 9002-10. [pdf] [html] Srikun, D.; Miller, E. W.; Domaille, D. W.; Chang, C. J., An ICT-based approach to ratiometric fluorescence imaging of hydrogen peroxide produced in living cells. J Am Chem Soc 2008, 130 (14), 4596-7. [pdf] [html] Miller, E. W.; He, Q.; Chang, C. J., Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging. Nat Protoc 2008, 3 (5), 777-83. [pdf] [html] Albers, A. E.; Dickinson, B. C.; Miller, E. W.; Chang, C. J., A red-emitting naphthofluorescein-based fluorescent probe for selective detection of hydrogen peroxide in living cells. Bioorg Med Chem Lett 2008, 18 (22), 5948-50. [pdf] [html] Yoon, S.; Miller, E. W.; He, Q.; Do, P. H.; Chang, C. J., A bright and specific fluorescent sensor for mercury in water, cells, and tissue. Angew Chem Int Ed Engl 2007, 46 (35), 6658-61. [pdf] [html] Miller, E. W.; Tulyathan, O.; Isacoff, E. Y.; Chang, C. J., Molecular imaging of hydrogen peroxide produced for cell signaling. Nat Chem Biol 2007, 3 (5), 263-7. [pdf] [html] Miller, E. W.; Chang, C. J., Fluorescent probes for nitric oxide and hydrogen peroxide in cell signaling. Curr Opin Chem Biol 2007, 11 (6), 620-5. [pdf] [html] Miller, E. W.; Bian, S. X.; Chang, C. J., A fluorescent sensor for imaging reversible redox cycles in living cells. J Am Chem Soc 2007, 129 (12), 3458-9. [pdf] [html] Zeng, L.; Miller, E. W.; Pralle, A.; Isacoff, E. Y.; Chang, C. J., A selective turn-on fluorescent sensor for imaging copper in living cells. J Am Chem Soc 2006, 128 (1), 10-1. [pdf] [html] Miller, E. W.; Zeng, L.; Domaille, D. W.; Chang, C. J., Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nat Protoc 2006, 1 (2), 824-7. [pdf] He, Q.; Miller, E. W.; Wong, A. P.; Chang, C. J., A selective fluorescent sensor for detecting lead in living cells. J Am Chem Soc 2006, 128 (29), 9316-7. [pdf] [html] Miller, E. W.; Albers, A. E.; Pralle, A.; Isacoff, E. Y.; Chang, C. J., Boronate-based fluorescent probes for imaging cellular hydrogen peroxide. J Am Chem Soc 2005, 127 (47), 16652-9. [pdf] [html] Heasley, V. L.; Fisher, A. M.; Herman, E. E.; Jacobsen, F. E.; Miller, E. W.; Ramirez, A. M.; Royer, N. R.; Whisenand, J. M.; Zoetewey, D. L.; Shellhamer, D. F., Investigations of the reactions of monochloramine and dichloramine with selected phenols: examination of humic acid models and water contaminants. Environ Sci Technol 2004, 38 (19), 5022-9. [pdf]