Chris Koon Ho WONG - 澳门大学

个人简介

Education PhD Department of Genetics, The University of Melbourne, Australia (2007) BSc (1st Class Hons) Department of Genetics, The University of Melbourne, Australia (2002) BSc Faculty of Science, The University of Melbourne, Australia (2001) Diploma Biotechnology, Ngee Ann Polytechnic, Singapore (1999) Academic and Professional Experience 2013-present Assistant Professor, Faculty of Health Sciences, University of Macau 2008-2013 Postdoctoral Research Fellow, Department of Biochemistry and Molecular Pharmacology, Harvard Medical School, USA 2007-2008 Postdoctoral Research Fellow, Department of Genetics, The University of Melbourne, Australia Course Outlines HSCI2004 Genetics and Epigenetics GEST1007 Fun in Health Science

研究领域

To understand how pathogenic fungi cause diseases in humans. To understand how transcription is regulated.

近期论文

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Representative Publications Journal Publication Choi, G.C.G., Zhou, P., Yuen, C.T.L., Chan, B.K.C., Xu, F., Bao, S., Chu, H.Y., Thean, D., Tan, K., Wong, K.H., Zheng, Z., Wong, A.S.L. “Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9” Nature Methods 2019. https://www.nature.com/articles/s41592-019-0473-0 Petrenko, N. *, Jin, Y. *, Dong, L., Wong, K.H. *# and Struhl, K#. “Requirements for RNA polymerase II preinitiation complex formation in vivo.” eLife 2019; 8:e43654. (* Equal contribution; # Corresponding author) https://doi.org/10.7554/eLife.43654 Kim, S.H., Iyer, K.R., Pardeshi, L., Munoz, J.F., Robbins, N., Cuomo, C.A., Wong, K.H., Cowen, L.E. “Genetic analysis of Candida auris implicates Hsp90 in morphogenesis and Azole tolerance and Cdr1 in Azole resistance.” MBio 2019; 10(1). pII:e02529-18. https://mbio.asm.org/content/10/1/e02529-18 Vassiliadis, D., Wong, K.H., Andrianopoulos, A., Monahan, B.J. “A genome-wide analysis of carbon catabolite repression in Schizosaccharomyces pombe.” BMC Genomics 2019; 20(1):251.https://doi.org/10.1186/s12864-019-5602-8 Qin, L., Li, A., Tan, K., Guo, S., Chen, Y., Wang, F., and Wong, K.H. “Universal plasmids to facilitate gene deletion and gene tagging in filamentous fungi.” Fungal Genetics and Biology 2019; 125:28-35. https://doi.org/10.1016/j.fgb.2019.01.004 Tan, K., and Wong, K.H. “RNA polymerase II ChIP-seq—a powerful and highly affordable method for studying fungal genomics and physiology.” Biophysical Reviews https://rdcu.be/bf1ib Zhang, B., Lyu, J., Liu, Y., Wu, C., Yang, E.J., Pardeshi, L., Tan, K., Wong, K.H., Chen, Q., Xu, X., Deng C. and Shim, J.S. “BRCA1 deficiency sensitizes breast cancer cells to bromodomain and extra-terminal domain (BET) inhibition.” Oncogene 2018; 37:6341-6356. https://doi.org/10.1038/s41388-018-0408-8< Sun, H., Miao, Z., Zhang, X., Chan, U.I., Su, S.M., Guo, S., Wong, K.H., Xu, X., and Deng. C.X. “Single-cell RNA-Seq reveals cell heterogeneity and hierarchy within mouse mammary epithelia.” Journal of Biological Chemistry 2018; 293(22) 8315–8329. http://www.jbc.org/content/early/2018/04/17/jbc.RA118.002297 Wang, H., Pardeshi, L.A., Rong, X., Li, E., Wong, K.H., Peng, Y., and Xu, R.H. “Novel variants identified in multiple sclerosis patients from southern China.” Frontiers in Neurology 2018; 9:582. https://doi.org/10.3389/fneur.2018.00582 Veri, A.O., Miao, Z., Shapiro, R.S., Tebbji, F., O’Meara, T.R., Kim, S.H., Colazo, J., Tan, K., Vyas, V.K., Whiteway, M., Robbins, N., Wong, K.H., and Cowen L.E. “Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space.” PLoS Genetics 2018; 14(3): e1007270. https://doi.org/10.1371/journal.pgen.1007270 Xie, J.L., Qin, L., Miao, Z., Grys, B.T., Diaz, J.D.L.C., Ting, K., Krieger, J.R., Tong, J., Tan, K., Leach, M.D., Ketela, T., Moran, M.F., Krysan, D.J., Boone, C., Andrews, B.J., Selmecki, A., Wong, K.H., Robbins, N. and Cowen, L.E. “The Candida albicans Transcription Factor Cas5 Couples Stress Responses, Drug Resistance, and Cell Cycle Regulation.” Nature Communications 2017; 8(1):499. https://www.nature.com/articles/s41467-017-00547-y Petrenko, N. *, Jin, Y. *, Wong, K.H. * and Struhl, K. “Evidence that Mediator is essential for Pol II transcription, but is not a required component of the preinitiation complex in vivo.” eLife 2017; 6:e28447. (* Equal contribution) https://doi.org/10.7554/eLife.28447.001 Jiang, B., Yan, L., Miao, Z., Li, E.-Q., Wong, K.H. and Xu, R.-H. “Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation.” Biomaterials 2017; 133:275-86. https://doi.org/10.1016/j.biomaterials.2017.03.050 Szot, P.S., Yang, A., Wang, X., Parsania, C., Rohm, U., Wong, K.H. and Ho, J.W.K. “PBrowse: a web-based platform for real-time collaborative exploration of genomic data.” Nucleic Acids Research. 2017; 45 (9): e67. https://doi.org/10.1093/nar/gkw1358 Petrenko, N. *, Jin, Y. *, Wong, K.H. *# and Struhl, K#. “Mediator undergoes a compositional change during transcriptional activation.” Molecular Cell. 2016; 64(3):443-454. (* Equal contribution; # Corresponding author) https://doi.org/10.1016/j.molcel.2016.09.015 Featured in: Malik, S., and Roeder, G.R. “Mediator: A Drawbridge across the Enhancer-Promoter Divide.” Molecular Cell. 2016; 64(3):433-434. https://doi.org/10.1016/j.molcel.2016.10.024 Leach, M.D., Farrer, R.A., Tan, K., Miao, Z., Walker, L.A., Cuomo, C.A., Wheeler, R.T., Brown, A.J.P., Wong, K.H. and Cowen, L.E. “Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodeling and chromatin architecture in Candida albicans.” Nature Communications. 2016 7:11704. https://www.nature.com/articles/ncomms11704 Wong, K.H. *, Jin, Y. *, and Struhl, K. “TFIIH phosphorylation of the Pol II CTD stimulates Mediator dissociation from the preinitiation complex and promoter escape.” Molecular Cell. 2014; 54(4): 601-612. (* Equal contribution) https://doi.org/10.1016/j.molcel.2014.03.024 Downes, D.J., Davis, M.A., Wong, K.H., Kreutzberger, S.D., Hynes, M.J., and Todd, R.B. “Dual DNA binding and coactivator functions of Aspergillus nidulans TamA, a Zn(II)2Cys6 transcription factor.” Molecular Microbiology. 2014 Jun;92(6):1198-211. https://doi.org/10.1111/mmi.12620 Hunter, C.C., Siebert, K.S., Downes, D.J., Wong, K.H., Kreutzberger, S.D., Fraser, J.A., Clarke, D.F., Hynes, M.J., Davis, M.A., and Todd, R.B. “Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA.” Eukaryotic Cell. 2014; 13(4):527-38. https://ec.asm.org/content/13/4/527 Wong, K.H.*, Jin, Y., and Moqtederi, Z. “Multiplex Illumina sequencing using DNA-barcoding.” Current Protocol in Molecular Biology. 2013; Chapter 7:Unit 7.11. (Invited protocol) (* Corresponding author) https://doi.org/10.1002/0471142727.mb0711s101 Suzuki, Y., Murray, S., Wong, K.H., Davis, M.A., and Hynes, M.J. “Reprogramming of carbon metabolism by the transcriptional activators AcuK and AcuM in Aspergillus nidulans.” Molecular Microbiology. 2012 84(5):942-64. https://doi.org/10.1111/j.1365-2958.2012.08067.x Wong, K.H., and Struhl, K. “The Cyc8−Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein.” Genes and Development. 2011 Dec 1; 25: 2525-2539. http://www.genesdev.org/cgi/doi/10.1101/gad.179275.111 Featured in: Parnell, E.J., and Stillman, D.J. “Shields up: the Tup1−Cyc8 repressor complex blocks coactivator recruitment.” Genes and Development. 2011 Dec 1, 25: 2429-2435. http://www.genesdev.org/cgi/doi/10.1101/gad.181768.111 Tirosh, I.*, Wong, K.H.*, Barkai, N., and Struhl, K. “Extensive divergence of yeast stress responses through transitions between induced and constitutive activation.” Proceedings of the National Academy of Sciences of USA. 2011 Oct 4; 108(40):16693-8. (* Equal contribution) https://doi.org/10.1073/pnas.1113718108 Suk, K., Choi, J., Suzuki, Y., Ozturk, SB., Mellor, JC., Wong, K.H., MacKay, JL., Gregory, R.I., and Roth, F.P. “Reconstitution of human RNA interference in budding yeast.” Nucleic Acids Research. 2011 Apr; 39(7):e43. https://doi.org/10.1093/nar/gkq1321 Fan, X., Geisberg, J.V., Wong, K.H., and Jin, Y., “Conditional depletion of nuclear proteins by the anchor away system.” Current Protocol in Molecular Biology. 2011 Jan; Chapter 13:Unit13.10B. (Invited protocol) https://doi.org/10.1002/0471142727.mb1310bs93 Wong, K.H., Hynes, M.J., Todd, R.B., and Davis, M.A. “Deletion and overexpression of the Aspergillus nidulans GATA factor AreB reveals unexpected pleiotropy.” Microbiology. 2009 Dec; 155(Pt 12):3868-80. https://dx.doi.org/10.1099/mic.0.031252-0 Wong, K.H., Hynes, M.J., and Davis, M.A. “Recent advances in nitrogen regulation: a comparison between yeast and filamentous fungi.” Mini-review. Eukaryotic Cell. 2008 7(6):917-925. https://ec.asm.org/content/7/6/917 Wong, K.H., Todd, R.B., Oakley, B.R., Oakley, C.E, Hynes, M.J., and Davis, M.A. “Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging.” Fungal Genetics and Biology. 2008 45:728-737. https://doi.org/10.1016/j.fgb.2007.12.009 Wong, K.H., Hynes, M.J., Todd, R.B., and Davis, M.A. “Transcriptional control of nmrA by the bZIP transcription factor MeaB reveals a new level of nitrogen regulation in Aspergillus nidulans.” Molecular Microbiology. 2007 66(2):534-551. https://doi.org/10.1111/j.1365-2958.2007.05940.x Todd, R.B., Fraser, J.A., Wong, K.H., Davis, M.A., and Hynes, M.J. “Nuclear accumulation of the GATA factor AreA in response to complete nitrogen starvation by regulation of nuclear export.” Eukaryotic Cell. 2005 4(10): 1646-1653. https://ec.asm.org/content/4/10/1646.long Book Chapter Davis, M.A. and Wong, K.H. “Chapter 23: Nitrogen Metabolism in Filamentous Fungi.” In: Cellular and Molecular Biology of Filamentous Fungi edited by Borkovich, Katherine A. and Ebbole, Daniel J. © 2010 American Society for Microbiology (ASM). (Invited book chapter)

学术兼职

Member of the Genetics Society of America