Barran, Perdita - The University of Manchester

个人简介

Perdita Barran is currently Chair of Mass Spectrometry in the School of Chemistry and Director of the Michael Barber Centre for Collaborative Mass Spectrometryr. She graduated from Manchester University with a degree in Chemistry with Industrial Experience (1994), and from Sussex University with a PhD in Chemical Physics (1998) under the supervision of Professors Tony Stace and Sir Harry Kroto. She worked as a Post-doctoral researcher for Tony Stace for 3 more years, before moving to University of California Santa Barbara to work with Mike Bowers. As an MRC Postdoctoral Fellow at the University of Edinburgh, she helped establish a Centre of Proteomics (SIRCAMS). Professor Barran was awarded an EPSRC Advanced Research Fellowship (March 2003) to study the structure and dynamics of model peptides and proteins in the gas phase. The Barran group have developed IM-MS instrumentation to investigate changes in protein conformation and aim to understand biological systems using mass spectrometry based techniques in conjunction with collaboration with biologists and biomedical research groups. In 2009 in recognition of her achievements Barran was awarded the inaugural Joseph Black award by the RSC Analytical Division.

研究领域

The Barran group has considerable experience in gas-phase ion chemistry and the application and development of mass spectrometry for complex chemical and biological problems. We use solvent free methodologies to provide an understanding of structure function relationships of proteins and peptides at the molecular level, and to use this information to further biomedical research. Our work has a particular focus on disordered and dynamic protein systems, weak interactions, and supramolecular systems. We aim to use mass spectrometry to solve problems that cannot be tackled well by other methods. Gentle application of nano electrospray to proteins buffered in solution to an appropriate pH allows us to use mass spectrometry to interrogate the gas phase version of their solution conformations. This can be achieved directly with ion mobility mass spectrometry, where we measure the rotationally averaged temperature dependent collision cross section of mass separated ions, or more indirectly with the use of dissociation methods and/or HDX. Data obtained from both methods provides insight to the structure and stability of the protein, and also detail on its interactions, especially when combined with atomistically resolved data from crystallography and or computational approaches. DT IM-MS is our central technique and one that we are currently developing a new higher resolution instrument for, however we employ several other biophysical methodologies.

近期论文

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Beveridge R, Covill S, Pacholarz K, Kalapothakis J, MacPhee C, Barran P. A mass-spectrometry-based framework to define the extent of disorder in proteins. Anal Chem. 2014; 86(22): 10979-91. eScholarID:247693 | DOI:10.1021/ac5027435 Harvey S, Porrini M, Konijnenberg A, Clarke D, Tyler R, Langridge-Smith P, MacPhee C, Volkman B, Barran P. Dissecting the dynamic conformations of the metamorphic protein lymphotactin. J Phys Chem B. 2014; 118(43): 12348-59. eScholarID:247695 | DOI:10.1021/jp504997k Kalapothakis J, Berezovskaya Y, Zampronio C, Faull P, Barran P, Cooper H. Unusual ECD fragmentation attributed to gas-phase helix formation in a conformationally dynamic peptide. Chem Commun (Camb). 2014; 50(2): 198-200. eScholarID:247696 | DOI:10.1039/c3cc46356g Kalapothakis J, Berezovskaya Y, Zampronio C, Faull P, Barran P, Cooper H. Unusual ECD fragmentation attributed to gas-phase helix formation in a conformationally dynamic peptide. Chemical Communications. 2014; 50(2): 198-200. eScholarID:216968 | DOI:Doi 10.1039/C3cc46356g Pacholarz K, Porrini M, Garlish R, Burnley R, Taylor R, Henry A, Barran P. Dynamics of intact immunoglobulin G explored by drift-tube ion-mobility mass spectrometry and molecular modeling. Angew Chem Int Ed Engl. 2014; 53(30): 7765-9. eScholarID:247694 | DOI:10.1002/anie.201402863 Ewa Jurneczko, Faye Cruickshank, Dr. Massimiliano Porrini, Dr. David J. Clarke, Dr. Iain D. G. Campuzano, Dr. Michael Morris, Dr. Penka V. Nikolova, Dr. Perdita E. Barran. Probing the Conformational Diversity of Cancer-Associated Mutations in p53 with Ion-Mobility Mass Spectrometry. Angewandte Chemie International Edition. 2013 April; 52(16): 4370-4374. eScholarID:208461 | DOI:10.1002/anie.201210015 Berezovskaya Y, Porrini M, Barran P. The effect of salt on the conformations of three model proteins is revealed by variable temperature ion mobility mass spectrometry. International Journal of Mass Spectrometry. 2013; 345: 8-18. eScholarID:216966 | DOI:DOI 10.1016/j.ijms.2013.02.005 Beveridge R, Chappuis Q, Macphee C, Barran P. Mass spectrometry methods for intrinsically disordered proteins. Analyst. 2013; 138(1): 32-42. eScholarID:216952 | DOI:Doi 10.1039/C2an35665a Jurneczko E, Cruickshank F, Porrini M, Clarke D, Campuzano I, Morris M, Nikolova P, Barran P. Probing the Conformational Diversity of Cancer-Associated Mutations in p53 with Ion-Mobility Mass Spectrometry. Angewandte Chemie-International Edition. 2013; 52(16): 4370-4374. eScholarID:216953 | DOI:DOI 10.1002/anie.201210015 Porrini M, Zachariae U, Barran P, MacPhee C. Effect of Protonation State on the Stability of Amyloid Oligomers Assembled from TTR(105-115). Journal of Physical Chemistry Letters. 2013; 4(8): 1233-1238. eScholarID:216937 | DOI:Doi 10.1021/Jz400372u Zhou Y, Webb S, Lettice L, Tardif S, Kilanowski F, Tyrrell C, Macpherson H, Semple F, Tennant P, Baker T, Hart A, Devenney P, Perry P, Davey T, Barran P, Barratt C, Dorin J. Partial deletion of chromosome 8 beta-defensin cluster confers sperm dysfunction and infertility in male mice. PLoS Genet. 2013; 9(10): e1003826. eScholarID:216956 | DOI:10.1371/journal.pgen.1003826