朱敏生 - 南京大学 - 模式动物研究所

个人简介

1995年博士毕业于中国科学院上海生化所。1995年1999年间，其在华东研究中心进行生物科学技术研究，并于1997年被任命为助理教授。之后，朱敏生博士在美国西南医学中心做博士后，并于2004年进入南京大学模式动物研究所任遗传学教授至今。基金种类基金项目名称国家自然科学基金　31272311　紧张性平滑肌自发张力形成的信号调控机制研究国家自然科学基金　31330034　平滑肌收缩高反应性的形成及其分子基础

研究领域

在系统生物学水平系统研究平滑肌收缩机制：平滑肌是机体中空器官的主要组成部分，其收缩功能是保证所在器官生理功能的基础。如收缩功能异常，则可引起一系列疾病，如胃肠疾病、心血管异常、支气管哮喘等。以往的研究主要通过比较钙离子、CaM、MLCK、MLCP等信号分子活性变化与收缩行为的相关性，以及应用酶抑制剂等方法研究收缩机制，但该方法存在有很大的局限性。为此，我们将分析MLCK、MYPT1、ZIPK和Trio条件性基因敲除小鼠平滑肌收缩特征和信号调控改变，系统研究平滑肌收缩的调控机制以及与平滑肌疾病的关系。神经发育与细胞运动的信号网络调控研究：发育信号与细胞运动信号的偶联调控是组织器官发育调控的一个基本过程，但其机制还不清楚。我们利用条件性基因敲除小鼠，研究探讨Trio-GEFs在小脑颗粒细胞运动过程的信号传递过程和机制. 肌肉、脂肪发育的分子生物学机制：主要研究miRNA对肌肉、脂肪发育分化的调控机制

近期论文

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1. Sun J., Yang GM., Tao T., Wei LS., PanY., Zhu MS. Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography. J. Vis. Exp. (138), e58064, doi:10.3791/58064 (2018) . 2. Berger SL, Leo-Macias A, Yuen S, Khatri L, Pfennig S, Zhang Y, Agullo-Pascual E, Caillol G, Zhu MS, Rothenberg E, Melendez-Vasquez CV, Delmar M, Leterrier C, Salzer JL. Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment. Neuron. 2018;97(3):555-570. 3. Yang GM, Sun J, Pan Y, Zhang JL, Xiao M, Zhu MS. Isolation and identi_ cation of a tribenzylisoquinoline alkaloid from Nelumbo nucifera Gaertn, a novel potential smooth muscle relaxant. Fitoterapia. 2018; 124:58-65. 4. Pei Wang, Wei Zhao, Jie Sun, Tao Tao, Xin Chen, Yan-Yan Zheng, Cheng-Hai Zhang, Zhong Chen, Yun-Qian Gao, Fan She, Ye-Qiong Li, Li-Sha Wei, Ping Lu, Cai-Ping Chen, Ji Zhou, Da-Quan Wang, Liang Chen, Xiao-Hao Shi, Linhong Deng, Ronghua ZhuGe, Hua-Qun Chen, Min-Sheng Zhu. Inflammatory Mediators Mediate Airway Smooth Muscle Contraction through a GPCR-TMEM16A-VDCC Axis and Contribute to Bronchial Hyperresponsiveness in Asthma. J Allergy Clin Immunol 2018;141(4):1259- 1268. 5. Xu S，Cao S, Zou B, Yue Y, Gu C, Chen X, Wang P, Dong X, Xiang Z, Li K, Zhu MS*, Zhao QS*, Zhou GH* Genome Biology 2016; 17:186-195. 6. Zhang CH, Wang P, Chen CP, Zhao W, Chen X, Chen C, He WQ, Qiao YN, Tao T, Sun J, Peng YJ, Craige SM, Lifshitz LM, Jr KJF, Fogarty KE, ZhuGe R, Zhu MS* The molecular basis underlying the genesis of basal tone in internal anal sphincter. Nature Communication 2016；7:11358. 7. Gao YQ, Chen X, Wang P, Lu L, Zhao W, Chen C, Chen CP, Tao T, Sun J, Zheng YY, Du Jie, Li CJ, Gan ZJ, Gao X, Chen HQ and Zhu MS*. Regulation of DLK1 by the maternally expressed miR-379-544 cluster may underlie callipyge polar overdominance inheritance. PNAS 2015; 112(44):13627-32. 8. Chen, C.P., Chen, X., Qiao, Y.N., Wang, P., He, W. Q., Zhang, C.H., Zhao, W., Gao, Y.Q., Chen, C., Tao, T., Sun, J., Wang, Y., Gao, N., Kamm, K.E., Stull, J.T., and Zhu, M.S * Roles in vivo for Myosin Phosphatase Targeting Subunit-1 T694 and T852 Phosphorylation sites in Bladder Smooth Muscle. J. Physiology 2015;593(3):681-700. 9. Chen C, Wen C, Tao T, He WQ, Qiao YN, Gao YQ, Wang P, Chen X, Chen HQ, Ye AP, Peng YJ, Zhu MS*. MLCK regulates cell migration in a RLC independent mechanism. J Biol Chem 2014;289(41):28478-88. 10. Qiao YN, He WQ, Chen CP, Zhang CH, Zhao W, Wang P, Zhang L, Yang X. Peng YJ, Gao JM, Kamm KE, Stull JT, Zhu MS* MYPT1 regulates vascular smooth muscle contractility and blood pressure maintenance. J Biol Chem 289, 22512-22523. 11. Wang Y, Zhao W, Zhang L, Zhao YN, Li F, Zhang Z, Dai YD, Li WF, Qiao YN, Chen CP, Gao JM, Zhu MS*. Molecular and cellular basis of the regulation of lymphatic contractility and lymphatic absorption. Int J Biochem Cell Biol. 2014；53:134-40. 12. He WQ, Qiao YN, Peng YJ, Zha JM, Zhang CH, Chen C, Chen CP, Wang P, Yang X, Li CJ,, Kamm KE, Stull JT, Zhu MS* Alteration of contractile phenotypes of intestinal smooth muscle from mice deficient in myosin phosphatase target subunit 1. Gastroenterology 2013；144：1450-1459. 13. Zhang CH, Li Y, Zhao W, Lifshitz LM, Li H, Harfe BD, Zhu MS*, ZhuGe R*. The TMEM16A Ca2+-activated Cl- channel in airway smooth muscle contributes to airway hyperresponsiveness. American Journal of Respiratory and Critical Care Medicine. 2013;187(4):374-813. 14. Zhang CH, Chen C, Lifshitz L, Fogarty K, Zhu MS* and ZhuGe R*. Correspondence: Bitter tastant-induced relaxation of airway smooth muscle does not involve BK channels. Nature Medicine 2012; 18(5):648-650. 15. He WQ, Qiao YN, Zhang CH, Peng YJ, Chen C, Wang P, Gao YQ, Chen CP, Chen X, Tao T, Su XH, Li CJ, Kamm KE, Stull JT, Zhu MS* Role of myosin light chain kinase in regulation of basal blood pressure and maintenance of salt-induced hypertension. Am J Physiology-Heart and Circulation Physiology 2011 AUG：301（2）：H584-H591. 16. Peng YJ, He WQ, Tang J, Tao T, Chen C, Zhang WC, Lv N, Zhao LP, Gao X, Zhu MS* (2009) Trio controls cerebellur neuron migration through neurite morphogenesis thereby regulates cerebellar development. J Biol Chem 2010：285（3）：24834-44. 17. Zhang WC, Peng YJ, Zhang GS, He WQ, Qiao YN, Dong YY, Gao YQ, Chen C, Zhang CH, Li W, Ning W, Kamm KE, Stull JT, Gao X and Zhu MS*. Myosin light chain is necessary for tonic airway smooth muscle contraction. J Biol. Chem. 2010;285:5522-31 . 18. He WQ， Peng YJ, Zhang WC, Lv N, Tang J, Chen C, Zhang CH, Gao S, Chen HQ, Zhi G, Feil R, Kamm KE, Stull JT, Gao X, Zhu MS* Myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice. Gastroenterology 2008;135：610-620. 19. 朱敏生主编：LAK细胞基础与临床，江苏省科学技术出版社