个人简介

Outstanding Educator Award (Student Alumni Association), 2001 Roche Research Foundation Award for Scientific Exchange, 1998 American Cancer Society (Calif. Div.) Senior Fellowship, 1987-1988 National Institute of Health Fellowship (declined

研究领域

Biochemistry/Physical/""Metabolism/Signaling/and Regulation""/Biophysics/Chemical Physics/Chemical Reaction Dynamics/Kinetics/Interactions/Energy Science/Instrument Development/Protein and Membrane Biochemis

Protein Structure, Function and Inhibition My group determines the atomic structures of proteins and seeks to understand how protein structure gives rise to protein function – both in vitro and in living cells. The problems we study have at their heart a fundamental structure-function question, but also address questions of importance to human health. Our approaches include X-ray crystallography, rapid kinetic measurements, spectroscopy, theory, protein expression, drug discovery, molecular genetics and related techniques. We are particularly interested in nitric oxide signaling mechanisms. Nitric oxide (NO) is a small reactive molecule produced by all higher organisms for the regulation of an immensely varied physiology, including blood pressure regulation, memory formation, tissue development and programmed cell death. We are interested in two NO signaling mechanisms: binding of NO to heme and the nitrosylation (nitrosation) of cysteines. NO, produced by NO synthase, binds to soluble guanylate cyclase (sGC) at a ferrous heme center, either in the same cell or in nearby cells. Binding leads to conformational changes in heme and protein, and to induction of the protein’s catalytic function and the production cGMP. NO can also react with cysteine residues in proteins, giving rise to S-nitroso (SNO) groups that can alter protein function. We are studying the mechanistic details surrounding cGMP and SNO production, and the signaling consequences of their formation. For reversible Fe-NO chemistry we are studying soluble guanylate cyclase and the nitrophorins, a family of NO transport proteins from blood-sucking insects. Our crystal structures of nitrophorin 4 extend to resolutions beyond 0.9 angstroms, allowing us to view hydrogens, multiple residue conformations and subtle changes in heme deformation. For reversible SNO chemistry, we are studying thioredoxin, glutathione S-nitroso reductase (GSNOR) and also sGC. For regulation in the cell, we have constructed a model cell system based on a human fibrosarcoma called HT-1080, where sGC, NO synthase, thioredoxin and GSNOR can be manipulated in a functional cellular environment. With these tools, we are exploring the molecular details of NO signaling and whole-cell physiology.

近期论文

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Fritz BG, Hu X, Brailey JL, Berry RE, Walker FA and Montfort WR. Oxidation and Loss of Heme in Soluble Guanylyl Cyclase from Manduca Sexta. Biochemistry (2011), 50:5813-5815. PMID: 21639146 Weichsel A, Kem M, Montfort WR. Crystal Structure of Human Thioredoxin Revealing an Unraveled Helix and Exposed S-Nitrosation Sit. Protein Sci. (2010), 19:1801-1806. PMCID: PMC2975143 Benabbas A, Ye X, Kubo M, Zhang Z, Maes EM, Montfort WR, Champion PM. Ultrafast Dynamics of Ligand Rebinding to Nitrophorin 4. J. Am. Chem. Soc. (2010), 132:2811-2820. PMCID: PMC2835772 Kubo M, Gruia F, Benabbas A, Montfort WR, Maes E, Champion PM. Low Frequency Mode Activity of Heme: Femtosecond Coherence Spectroscopy of Iron Porphine Halides and Nitrophorin. J. Am. Chem. Soc. (2008), 130:9800-9811. PMCID: PMC2765994 Hu X, Feng C, Hazzard JT, Tollin G, Montfort WR. Bindin of YC-1 or BAY 41-2272 to Soluble Guanylyl Cyclase Induces a Geminate Phase for Photolyzed Co. J. Am. Chem. Soc. (2008), 130:15748-15749. PMCID: PMC2645941 Hu X, Murata L, Weichsel A, Brailey JL, Roberts SA, Nighorn A, Montfort WR. Allostery in Recombinant Soluble Guanylyl Cyclase from Manduca Sexta. J. Biol. Chem. (2008), 283:20968-20977. PMCID: PMC2475682 Amoia AM and Montfort WR. Apo-Nitrophorin 4 at Atomic Resolution. Protein Sci. (2007), 16:2076-2081. PMCID: PMC2206961 Schreiter ER, Rodriguez MM, Weichsel A, Montfort WR, Bonaventura, J. S-Nitrosylation-Induced Conformational Change in Blackfin Tuna Myoglobin. J. Biol. Chem. (2007), 282:19773-19780. PMID: 17488722 Weichsel A, Brailey JL, and Montfort WR. Buried S-Nitrosocysteine Revealed in Crystal Structures of Human Thioredoxin, Biochemistry 46 (2007), 1219-1227. PMID: 17260951 Kondrashov DA, and Montfort WR. Nonequilibrium dynamics simulations of nitric oxide release: comparative study of nitrophorin and myoglobin. J. Phys. Chem. B (2007), 111:9244-9252. PMID: 17622170 Weichsel A, Maes EM, Andersen JF, Valenzuela JG, Shokhireva T, Walker FA, and Montfort WR. Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein, Proc. Natl. Acad. Sci. USA 102 (2005), 594-599. PMCID: PMC545542 Maes EM, Roberts SA, Weichsel A, and Montfort WR. Ultrahigh Resolution Structures of Nitrophorin 4: Heme Distortion in Ferrous CO and NO Complexes, Biochemistry 44 (2005), 12690-12699. Singh SK, Grass G, Rensing C, and Montfort WR. Cuprous oxidase activity of CueO from Escherichia coli, J. Bacteriol. 186 (2004), 7815-7817. Kondrashov DA, Roberts SA, Weichsel A, and Montfort WR. Protein functional cycle viewed at atomic resolution: conformational change and mobility in nitrophorin 4 as a function of pH and NO binding, Biochemistry 43 (2004), 13637-13647. Roberts SA, Weichsel A, Grass G, Thakali K, Hazzard JT, Tollin G, Rensing C, and Montfort WR. Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli, Proc. Natl. Acad. Sci. USA(2002), 99:2766-2771. PMCID: PMC122422 Weichsel A, Andersen JF, Roberts SA., and Montfort WR. Reversible nitric oxide binding to nitrophorin 4 from Rhodnius prolixus involves complete distal pocket burial, Nat. Struct. Biol. (2000), 7:551-554. PMID: 10876239 Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR. Crystal Structures of a Nitric Oxide Transport Protein from a Blood-Sucking Insect. Nature Struct. Biol. (1998), 5:304-309. PMID: 9546222 Weichsel A, Montfort WR. Ligand-induced distortion of an active site in thymidylate synthase upon binding anticancer drug 1843U89. Nature Struct. Biol. (1995), 2:1095-1101. PMID: 8846221