个人简介

UA Honors College Faculty Excellence Award, 2012 Chair, American Cancer Society TBE Panel, 1999-2001 Chair, Gordon Conference on Cancer, 1994 Fellow & Scholar, Leukemia Society America, 1986-1994 Fellow, Jane Coffin Childs Memorial Fund, 1

研究领域

Biochemistry/""Metabolism/Signaling/and Regulation""“

Blood Meal Metabolism in Mosquitoes Mosquitoes are human disease vectors that transmit pathogens through blood feeding. These human pathogens include the malarial parasite, Dengue and yellow fever viruses, and West Nile virus. Dengue fever and malaria continue to have a significant health and economic impact worldwide, and therefore new approaches are needed for controlling the spread of these diseases. Mosquitoes are highly evolved blood sucking insects that have been shown to efficiently ingest, metabolize, and transport blood meal nutrients from the midgut lumen to the ovaries to produce eggs. Although we have a basic knowledge of these processes at the physiological level, it requires a molecular understanding of metabolic regulation in mosquitoes in order to target blood meal metabolism as transmission blocking or vector control strategy. Since highly coordinated endocytotic and exocytotic processes in midgut, fat body, and ovary tissues likely play a central role in metabolic flux following the blood meal, we are investigating vesicle transport in Aedes aegypti and Anopheles stephensi mosquitoes.

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Isoe, J., Mack, D.J., Njardarson, J.T., and Miesfeld, R.L. Biochemical inhibitors of COPI vesicle transport have differential metabolic effects on Diptera insects, in preparation. Isoe, J., Stover, W., Miesfeld, R.B. and Miesfeld, R.L. (2013) COPI-mediated blood meal digestion in vector mosquitoes is independent of midgut ARF-GEF and ARF-GAP regulatory activities., Insect. Bioc. Mol. Biol., 43:732-739. Mack, D.J., Isoe, J., Miesfeld, R.L. and Njardarson, J.T. (2012) Distinct biological effects of Golgicide A derivatives on larval and adult mosquitoes, Bioorg. Med. Chem. Lett., 22:5177-5188. Alabaster, A., Isoe, J., Zhou, G., Lee, A., Murphy, A. and Miesfeld, R. (2011) Deficiencies in acetyl-CoA carboxylase and fatty acid synthase 1 differentially affect eggshell development and blood meal digestion in Aedes aegypti. Insect. Bioc. Mol. Biol., 41:946-955. Rascon, A., Gearin, J., Isoe, and Miesfeld, R. (2011) In vitro activation and enzyme kinetic analysis of recombinant midgut serine proteases in the Dengue vector mosquito Aedes aegypti. BMC Bioc. 12:43. Isoe, J., Collins, J., Badgandi, H., Day, W.A., and Miesfeld, R. (2011) Defects in COPI transport cause blood feeding-induced mortality in Yellow Fever mosquitoes, Proc. Nat. Acad. Sci., 108:E211-E217. Zhou, G., Isoe, J., Day, W.A., and Miesfeld, R. (2011) Alpha-COPI transport function is required for rough endoplasmic reticulum whorl formation in mosquito midgut epithelial cells, PLoS ONE 6(3): e18150. Scaraffia, P.Y., Zhang, Q., Thorson, K., Wysocki, V.H. and Miesfeld, R. (2010) Differential ammonia metabolism Aedes aegypti fat body and midgut tissues, J. Insect. Physiol., 56:1040-1049. Wei, J., Wysocki, V.H., Miesfeld, R. and Scaraffia, P.Y. (2010) Differentiation and quantification of C1 and C2 13C-labeled glucose by tandem mass spectrometry, Analytical. Bioc., 404:40-44. Brackney, D.E., Isoe, J., Zamora, J., Black, W.C., Foy, B.D., Miesfeld, R. and Olson, K.E. (2010) Expression profiling and comparative analysis of seven novel midgut serine proteases from the Yellow Fever mosquito, J. Insect. Physiol. 56:736-744. Isoe, J., Rascon, A., Kunz, S., and Miesfeld, R. (2009) Molecular genetic analysis of midgut serine proteases in Aedes aegypti mosquitoes, Insect Bioc. Mol. Biol., 39:903-912. Zhou, G. and Miesfeld, R. (2009) Differential utilization of blood meal amino acids in Aedes aegypti mosquitoes, Open Access Insect Physiology, 1:1-12. Zhou, G. and Miesfeld, R. (2009) Energy metabolism during diapause in Culex pipiens mosquitoes. J. Insect. Physiol., 55:40-46. Isoe, J., Zamora, J. and Miesfeld, R. (2009) Molecular analysis of the Aedes aegypti carboxypeptidase gene family. Insect. Bioc. Mol. Biol., 39:68-73. Scaraffia, P.Y., Tang, G., Isoe, J., Wysocki, V.H., Wells, M.A. and Miesfeld, R. (2008) Discovery of an alternate metabolic pathway for urea synthesis in adult Aedes aegypti mosquitoes, Proc. Nat. Acad. Sci., 105:518-523. Brandon, M.C., Pennington, J.E., Zamora, J., Isoe, J., Schillinger, A-S. and Miesfeld, R. (2008) TOR signaling is required for amino acid stimulation of early trypsin protein synthesis in the midgut of Aedes aegypti mosquitoes. Insect. Bioc. Mol. Biol., 38:916-922. Isoe, J., Kunz, S., Manhart, C., Wells, M.A. and Miesfeld, R. (2007) Regulated expression of microinjected DNA in adult Aedes aegypti mosquitoes. Insect Mol. Biol., 16:83-92.