个人简介

Diploma Engineer in Chemistry, April 1986, Lomonosov Institute of Fine Chemical Technology, Moscow, USSR Ph. D., Physical Chemistry, University of Illinois, 1997 Postdoctoral Fellow (NIH Individual National Research Service Award), 1997-2000 Illinois EPR Research Center, University of Illinois at Urbana -Champaign, Urbana, IL

研究领域

Physical Chemistry

My research interests are focused on application of spectroscopy, especially electron paramagnetic resonance (EPR) and fluorescence, in chemistry and biology, specifically to study structure and dynamics of membranes and proteins, drug-protein, and protein-protein interactions. Site-directed spin labeling of proteins: measuring protein distances in solution, protein folding/unfolding; interactions of protein with drugs and environment; docking of fatty acids and drug molecules in lipophilic protein channels. Dynamics of lipids and lipid-protein interactions. Pico-to-nanosecond dynamics of small molecules in complex systems. Characterization of transient free radicals by spin trapping EPR. Electron spin exchange and dipolar interactions in liquids and low-dimensional magnetic systems including metallo-organic ferromagnets: theoretical and experimental aspects. Magnetic susceptibility and EPR studies of low-dimensional systems. Current project focuses on understanding molecular aspects of binding and interactions of MRI paramagnetic contrast agents (PCA) molecules with several key biomolecules, such as blood pool proteins and phospholipids, and the relationship between structure of PCA's and their binding properties and proton relaxation enhancement. The goal will be achieved by investigating these interactions and their effects on structure and electronic properties of PCAs' by variety of spectroscopic techniques, including fluorescence, NMR, and EPR at multiple frequencies.

近期论文

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T. I. Smirnova, A. I. Smirnov, S. Pachtchenko, O. G. Poluektov, "Geometry of Hydrogen Bonds Formed by Lipid Bilayer Nitroxide Probes: A High Frequency Pulsed ENDOR/EPR Study", J. Am. Chem. Soc., Communication, accepted (2007). T.I. Smirnova, T. G. Chadwick, M.A. Voinov, O. Poluektov, J. van Tol, A. Ozarowski, G. Schaaf, M. M. Ryan, and V. A. Bankaitis "Local Polarity and Hydrogen Bonding inside the Sec14p Phospholipid-binding Cavity: High-Field Multifrequency EPR Studies" Biophysical J. accepted (2007) T.I Smirnova "Binding of MRI Contrast Agents to Albumin: a High-Field EPR Study" Appl. Magn. Res. Accepted (2007) T. I Smirnova and Alex I. Smirnov, "High-field ESR Spectroscopy In Membrane And Protein Biophysics", in "ESR Spectroscopy in Membrane Biophysics", Biological Magnetic Resonance, Vol. 27, Hemminga, Marcus A., and Berliner, Lawrence Eds. Springer, New York, ISBN: 0-387-25066-2 (2007). Smirnova, T.I., Chadwick, T.G., MacArthur, R., Poluektov, O., Song, L., Ryan, M.M., Schaaf, G., and Bankaitis, V.A. "The Chemistry of Phospholipid Binding by the Saccharomyces cerevisiae Phosphatidylinositol Transfer Protein Sec14p as Determined by Electron Paramagnetic Resonance Spectroscopy" J. Biolog. Chem. 281(46), 34897-34908 (2006). Smirnov, A. I., Smirnova, T. I., MacArthur, R., Hall, R. "Cryogen-Free Superconducting Magnet System for Multifrequency EPR up to 12 T", Rev. Sci. Instrum. 77, 035108 (2006). Smirnova, T.I., Guenther, R., Lommel, S. A. "Dynamic changes in virions triggered by divalent metal ions" Biophys. J. 2006 Smirnova, T. I, Chadwick, T. G.,. Bankaitis, V. A. "Multifrequency EPR studies of mobility and local polarity of a protein-bound PC lipid" Biophys. J., v.88, p.45 (2005). Smirnov, A. I., and Smirnova, T. I. "Convolution-Based Algorithm: from Motional Analysis to EPR Oximetry and Protein Distance Measurements", Biological Magnetic Resonance, (L. J. Berliner, Ed.), vol. 21, pp 277-348 (2004). Smirnova, T. I., Roach, B. "Unfolding of Concanavalin A: a View from Mn(II) Binding Center", Biophys. J., v. 86,1, p345a (2004). Smirnova T. I., Smirnov, A.I "Dynamic Molecular Oxygen Accessibility to a Buried Mn2+ Protein Site: a High Field EPR Experiment", J. Phys. Chem., 107(29): 7212-7215 (2003). Smirnov, A. I., Smirnova T. I. "Resolving Domains of Interdigitated Phospholipid Membranes with 95 GHz Spin Labeling EPR", Appl. Magn. Reson. v. 21, pp. 453-467 (2001). Clarkson, R. B, Smirnov, A. I., Smirnova, T. I., Belford, R. L. "Multi-Frequency EPR of High-Spin S-state Ions: Examples from Gd3+ Chelates, in The Chemistry of Contrast Agents, In Medical Magnetic Resonance Imaging", A. Merbach, ed., Chapter 9; Wiley, New York, pp. 383-415 (2001). Smirnova, T. I., Smirnov, A. I., Belford, R. L., Clarkson, R. B. "Interaction of Gd(III) MRI Contrast Agent with Membranes: a Review of Recent EPR Studies", Magn. Reson. Materials In Physics, Biology and Medicine 8, 214-229, (1999). Smirnova, T. I., Smirnov, A. I., Clarkson, R. B., Belford, R. L. "Lipid-MRI Contrast Agent Interactions: a Spin-Labeling and a Multifrequency EPR Study", J. Am. Chem. Soc. 120, 5060-5072, (1998).