孙博 - 上海科技大学 - 生命科学与技术学院

个人简介

教育经历 2000/09—2004/06，吉林大学，学士 2004/09—2009/06，中国科学院物理研究所，博士博士后及工作经历 2009/07—2015/06，美国康奈尔大学/霍华德休斯医学研究所，博士后 2015/08—2021/07，上海科技大学，生命科学与技术学院，助理教授（TENURE-TRACK） 2021/07—至今，上海科技大学，生命科学与技术学院，副教授（TENURED）

研究领域

单分子酶学，单分子生物物理学应用光镊、磁镊以及荧光共振能量转移等单分子技术围绕DNA复制、修复、重组、编辑及分离等核酸代谢过程进行重要分子机器（例如：解旋酶，聚合酶，核酸酶等）的动力学解析，理解它们的分子机制、功能及协作模式，为治疗由该类蛋白异常引起的相应疾病提供分子生物学基础。

近期论文

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Bloom syndrome helicase compresses single-stranded DNA into phase-separated condensates Wang, T.#, Hu, J.J.#, Li, Y.N., Bi, L.L., Guo, L.J., Jia, X.S., Zhang, X., Li, D., Hou, X.M., Modesti, M., Xi, X.G., Liu, C.*, and Sun, B.* Angewandte Chemie International Edition 61, e202209463. (2022). Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis Guo, L.J.#, Zhao, Y.L.#, Zhang, Q., Feng, Y., Bi, L.L., Zhang, X., Wang, T., Liu, C., Ma, H.H., and Sun, B.* Nucleic Acids Research 50, 9101-9112. (2022). Simultaneous mechanical and fluorescence detection of helicase-catalyzed DNA unwinding Bi, L.L., Qin, Z.H., Hou, X.M., Modesti, M., and Sun, B.* Methods in Molecular Biology 2478, 329-347. (2022). PCDetection: PolyA-CRISPR/Cas12a-based miRNA detection without PAM restriction Zhong, M.T.#, Chen, K.Z.#, Sun, W.J., Li, X.Y., Huang, S.S., Meng, Q,Z., Sun, B., Huang, X.X.*, Wang, X.J.*, Ma, X.D.*, and Ma, P.X.* Biosensors and Bioelectronics 214, 114497. (2022). Structural mechanism underpinning Thermus oshimai Pif1-mediated G-quadruplex unfolding Dai, Y.X.#, Guo, H.L.#, Liu, N.N.#, Chen, W.F., Ai, X., Li, H.H., Sun, B., Hou, X.M.*, Rety, S.*, and Xi, X.G.* EMBO Reports 23, e53874. (2022). The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition Bi, L.L.#, Qin, Z.H.#, Wang, T., Li, Y.N., Jia, X.S., Zhang, X., Hou, X.M., Modesti, M., Xi, X.G., and Sun, B.* Proceedings of the National Academy of Sciences 119, e2116462119. (2022). Remodeling the conformational dynamics of i-motif DNA by helicases in ATP-independent mode at acidic environment Gao, B., Zheng, Y.T., Su, A.M., Sun, B., Xi, X.G., and Hou, X.M.* iScience 25, 103575. (2022). Molecular mechanisms of Streptococcus pyogenes Cas9: a single-molecule perspective Zhang, Q., Chen, Z.T., and Sun, B.* Biophysics Reports 7, 475-489. (2021). Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer Zhang, Q.#, Chen, Z.T.#, Wang, F.Z., Zhang, S.Q., Chen, H.Y., Gu, X.Y., Wen, F.C., Jin, J.C., Zhang, X., Huang, X.X., Shen, B.*, and Sun, B.* Nucleic Acids Research 49, 12433-12444. (2021). The hereditary mutation G51D unlocks a distinct fibril strain transmissible to wild-type α-synuclein Sun, Y.P.#, Long, H.F.#, Xia, W.C., Wang, K., Zhang, X., Sun, B., Cao, Q., Zhang, Y.Y., Dai, B., Li, D., and Liu, C.* Nature Communications 12, 6252. (2021). Replication protein A plays multifaceted roles complementary to specialized helicases in processing G-quadruplex DNA Wang, Y.R., Guo, T.T., Zheng, Y.T., Lai, C.W., Sun, B., Xi, X.G., and Hou, X.M.* iScience 24, 102493. (2021). Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungi Zhai, L.T.#, Rety, S.#, Chen, W.F.#, Song, Z.Y., Auguin, D., Sun, B., Dou, S.X., and Xi, X.G.* Nucleic Acids Research 49, 4768-4781. (2021). The structure of a minimum amyloid fibril core formed by necroptosis-mediating RHIM of human RIPK3 Wu, X.L.#, Ma, Y.Y.#, Zhao, K.#, Zhang, J., Sun, Y.P., Li, Y.C., Dong, X.Q., Hu, H., Liu, J., Wang, J., Zhang, X., Li, B., Wang, H.Y., Li, D., Sun, B., Lu, J.X.*, and Liu, C.* Proceedings of the National Academy of Sciences 118, e2022933118. (2021). Proximal single-stranded RNA destabilizes human telomerase RNA G-quadruplex and induces its distinct conformers Ye, S.S.#, Chen, Z.T.#, Zhang, X., Li, F.F., Guo, L.J., Hou, X.M., Wu, W.Q., Wang, J., Liu, C., Zheng, K., and Sun, B.* The Journal of Physical Chemistry Letters 12, 3361-3366. (2021). A novel partially-open state of SHP2 points to a “multiple gear” regulation mechanism Tao, Y.Q.#, Xie, J.F.#, Zhong, Q.L.#, Wang, Y.Y.#, Zhang, S.N., Luo, F., Wen, F.C., Xie, J.J., Zhao, J.W., Sun, X., Long, H.F., Ma, J.F., Zhang, Q., Long, J.G., Fang, X.Y., Lu, Y., Li, D., Li, M., Zhu, J.D., Sun, B., Li, G.H.*, Diao, J.J.*, and Liu, C.* Journal of Biological Chemistry 296, 100538. (2021). The nuclear localization sequence mediates hnRNPA1 amyloid fibril formation revealed by cryoEM structure Sun, Y.P.#, Zhao, K.#, Xia, W.C., Feng, G.Q., Gu, J.G., Ma, Y.Y., Gui, X.R., Zhang, X., Fang, Y.S., Sun, B., Wang, R.X., Liu, C.*, and Li, D.* Nature Communications 11, 6349. (2020). The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex Teng, F.Y., Wang, T.T., Guo, H.L., Xin, B.G., Sun, B., Dou, S.X., Xi, X.G.*, and Hou, X.M.* Journal of Biological Chemistry 295, 17646-17658. (2020). Conformational dynamics of nonenveloped circovirus capsid to the host cell receptor Li, J.R.#, Gu, J.Y.#,*, Lin, C., Zhou, J.W., Wang, S.N., Lei, J., Wen, F.C., Sun, B., and Zhou, J.Y.* iScience 23, 101547. (2020). Dynamics of Staphylococcus aureus Cas9 in DNA target association and dissociation Zhang, S.Q.#, Zhang, Q.#, Hou, X.M., Guo, L.J., Wang, F.Z., Bi, L.L., Zhang, X., Li, H.H., Wen, F.C., Xi, X.G., Huang, X.X., Shen, B., and Sun, B.* EMBO Reports 21, e50184. (2020). Human RPA activates BLM’s bidirectional DNA unwinding from a nick Qin, Z.H.#, Bi, L.L.#, Hou, X.M., Zhang, S.Q., Zhang, X., Lu, Y., Li, M., Modesti, M., Xi, X.G.*, and Sun, B.* eLife 9, e54098. (2020).