研究领域
TheZhonglabattheMaterialsandPhysicalBiologyDivisionintheSchoolofPhysicalScience&Technology(SPST)aimstoleveragethepowerofsyntheticbiologytodevelopanintegrativebio-inspiredmolecularengineering(IBME)program,throughwhichnewmaterials,nanotechnologiesanddevicesarecreatedbyadaptingandapplyingthewaysthatlivingorganismsdevelopandfunction.Theultimategoalofourresearchistocreatestimuli-responsive,self-regeneratingandlife-enhancingfunctionalmaterialsanddevicesbyintegratingsyntheticbiology,microbialengineering,materialsscience,nanotechnologyandmodernmanufacturingtools.
Ourcurrentresearcheffortsaremainlyfocusedonstructuralamyloids,emergingmolecularmaterialswithattributesincludingintrinsicself-assembly,ultra-stability,outstandingmechanicalpropertiesandtunablefunctionalities.Traditionallyconsideredasmis-foldedstructuresrelevanttoseveralneurodegenerativediseasesinmammals,amyloidshavebeenincreasinglyrecognizedasfunctionalbuildingblocksinorganisms.Forexample,theyarecloselyassociatedwiththecatalysisofmelaninsynthesisinmammalianmelanosomes;theyalsoconstitutetheprimarystructuralproteinsinbacterialbiofilms,andfunctionalcomponentsofunderwateradhesivesinmarinespecies.
Takinginspirationfrombiology,ourresearchprograminharnessingfunctionalamyloidsfornewbiomaterialsandbionanotechnologiescomprisefourmainthrusts:
(1)LivingFunctionalMaterials;
(2)Bio-inspiredUnderwaterAdhesives;
(3)DynamicComplexAssembliesandBiofabrication;
(4)Biofilms-interfacedBiocatalysis&ArtificialPhotosynthesis.
Ourresearchiscommittedtoadvancingtheemergingfieldofsyntheticbiologyandpushingnewfrontiersinbiomaterialsresearchandbionanotechnologies.
(1)LivingFunctionalMaterials
EngineeringLivingFunctionalMaterialswithSyntheticBiology
Livingfunctionalmaterialsrepresentaconceptuallynewtypeofmaterialsthatpossessmultifunctional,self-healingandadaptableproperties,createdandorganizedinadistributed,bottom-upandenvironmentallysustainablemanner.Byleveragingthepowerofsyntheticbiology,wearedevelopingaprogrammablelivingfunctionalmaterialplatformbasedonbacterialbiofilmsystems.
Ourcurrenteffortsalongthislineinclude:
1)programmingE.colicellstoorganizeinorganicnano-objects(NOs)inaspatiotemporallyprecisefashionwithlight-inducibleandchemical-induciblegenecircuits(X.Wangetal.AdvancedMaterials2018);
2)reprogrammingBacillussubtilisextracelluarmatrixproteinwithnewfunctionalpropertiesbygeneticallyappendingpeptidedomainsorproteinstotheamyloid-likeproteinTasAandleveragingtheintrinsicproteinsecretionpathway(J.Huangetal.NatureChemicalBiology2018).
3)engineeringLivingcellularglueswithtunableadhesionperformances,environmentalresponsiveness,andautonomousrepairingfeatures.(C.Zhangetal.MaterialsToday2019)
Asanotherexample,werecentlydemonstratedthatTasAvariantshavingfusionproteinsaslargeas630aminoacidscouldbesecretedandassembleintonanofibernetworks,outperformingthepreviouslyreportedE.coliCsgAsystem(60aminoacids).Furthermore,thelivingbiofilmscanbeengineeredwithtunableviscoelasticpropertiesandthereforeareprintablewiththree-dimensional(3D)printingtechnique.Notably,thebio-manufacturedlivingfunctionalbiofilmsremainaliveandareabletosensechemicalsignals,servingaslong-lastingenvironmentallyresponsivematerials.Theprogrammablelivingfunctionalmaterialplatformpromisesnewopportunitiesinbiocatalysis,bioremediation,bioadhesionandbiomedicine.
(2)Bio-inspiredUnderwaterAdhesives
(3)ComplexAssemblies&Bio-fabrication
Biologicalmaterialsandsystemsarerepletewithdiverseself-assemblies,includingbothnon-equilibriumandequilibriumsystems.Self-assembliesconstitutethebasisofmanycomplexsupramolecularstructures,providingextraordinaryfunctionalpropertiesandplayingimportantphysiologicalrolesforvariousmolecularmaterialsandbiologicalmachines.Characteristicofsuchbiologicalself-assembliesisitsdynamic,adaptive,andenvironmentallyresponsivenature,providinganunparalleledpotentialforafuturerepertoireofmultifunctionalmaterialsandnanotechnologies.Inherenttomanybiologicalassembliesistheadditionalfeatureofself-replication:DNAfromDNA;cellsfromcells.
Inmimickingthediversecomplexassemblingsystemsinnature,weleveragedtheintrinsicself-assemblingpropensityofthefusedinsarcoma(FUS)lowcomplexity(LC)domain,alongwithamodulargeneticstrategy,toconstructtailor-designedsupramolecularstructureswithvariablefunctionalities.Byfinelycontrollingthesequentialassembliesandassociatedassemblykinetics,ourstrategywassuitableforcreatingrandomcopolymer-like,multi-block,andself-sortedsupramolecularfibersthatcaneitherdisplaydistinctfluorescentfunctionalitiesorallowthespatiallycontrolledassemblyofnano-objectsinstructure-dependentfashions(ACSNano2017).
InE.colibiofilms,theoutermembrane-localizedamyloidgenicCsgBproteinscouldactasnucleatorstoguidetheefficientpolymerizationofthemajorcurlifibersubunitproteinCsgAintoamyloidfibersonthecellsurface.Inspiredbysuchnucleation-directedassemblyofamyloidfibrilsinbacterialbiofilms,wedevelopedaCsgB-decoratedDNAorigami(CB-origami)tomimicproteinnucleatorsinEscherichiacolibiofilmthatdirectcurlipolymerization.WeshowedthatCB-origamidirectedcurlisubunitCsgAmonomerstoformoligomersandthenacceleratedfibrilformationbyincreasingtheproliferationrateofprimarypathways.Fibrilsgrowedeitheroutfrom(departuremode)ortowardsthenucleators(arrivalmode),implyingtwodistinctrolesofCsgB:asnucleationsitesandastrapsitestocapturegrowingnanofibrilsinvicinity.Thisorigaminucleatorthusprovidedaninvitroplatformformechanisticallyprobingmolecularnucleationandcontrollingdirectionalfibrilpolymerizationforbionanotechnology.(NatureCommunications2019)
(4)Biofilms-interfacedBiocatalysis&ArtificialPhotosynthesis
近期论文
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#Equalcontribution，*Correspondingauthor
L.Nie#,Y.Li#,S.Chen,K.Li,Y.Huang,Y.Zhu,Z.Sun,J.Zhang,Y.He,M.Cui,S.Wei,F.Qiu,C.Zhong*,andW.Liu*,BiofilmNanofiber-CoatedSeparatorsforDendrite-FreeLithiumMetalAnodeandUltrahigh-RateLithiumBatteries,ACSAppl.Mater.Interfaces,2019,11,35,32373-32380.DOI:10.1021/acsami.9b08656
Y.Li,C.Zhong*etal,PatternedAmyloidMaterialsIntegratingRobustnessandGeneticallyProgrammableFunctionality,NanoLetters,2019.DOI:10.1021/acs.nanolett.9b02324
F.Tan#*,C.Chu#,J.Qi#,W.Li#,D.You,K.Li,X.Chen,W.Zhao,C.Cheng,X.Liu,Y.Qiao,B.Su,S.He,C.Zhong,H.Li*,R.Chai*&G.Zhong*,AAV-ieenablessafeandefficientgenetransfertoinnerearcells,NatureCommunications,2019,10,3733.DOI:10.1038/s41467-019-11687-8
X.Wang,C.Zhong*etal,ImmobilizationofFunctionalNano-objectsinLivingEngineeredBacterialBiofilmsforCatalyticApplications,NationalScienceReview,nwz104,DOI:org/10.1093/nsr/nwz104.
M.Cui,C.Zhong*etal,ExploitingMammalianLowComplexityDomainsforLiquid-liquidPhaseSeparationDrivenUnderwaterAdhesiveCoatings,ScienceAdvances,2019,5(8),eaax3155.
Y.Liu#,J.Qi#,X.Chen#,M.Tang#,C.Chu,W.Zhu,H.Li,C.Tian,G.Yang,C.Zhong,Y.Zhang,G.Ni,S.He*,R.Chai*andG.Zhong*,Criticalroleofspectrininhearingdevelopmentanddeafness,ScienceAdvances,2019,5(4),eaav7803.DOI:10.1126/sciadv.aav7803
X.Mao,K.Li,M.Liu,X.Wang,T.Zhao,B.An,M.Cui,Y.Li,J.Pu,J.Li,L.Wang,T.K.Lu,C.Fan*,andC.Zhong*.DirectingCurliPolymerizationwithDNAOrigamiNucleators.Naturecommunications2019,10,1395.DOI:10.1038/s41467-019-09369-6.
M.Cui#,Q.Qi#,T.Gurry#,T.Zhao,B.An,J.Pu,X.Gui,A.Cheng,S.Zhang,D.Xun,M.Becce,F.Vangosa,C.Liu,T.K.Lu,C.Zhong*GeneticDesignofMulti-domainFunctionalAmyloids:InsightsintoSelf-assemblyandFunctionalProperties,ChemicalScience2019,10,4004-4014.DOI:10.1039/C9SC00208A.
C.Zhang#,J.Huang#,J.Zhang#,S.Liu,M.Cui,B.An,X.Wang,J.Pu,T.Zhao,C.Fan,T.K.Lu,C.Zhong*,EngineeredBacillussubtilisbiofilmsaslivingglues,MaterialsToday2019,28,40-48.DOI:10.1016/j.mattod.2018.12.039.
J.Huang#,S.Liu#,C.Zhang#,X.Wang,J.Pu,F.Ba,S.Xue,H.Ye,T.Zhao,K.Li,Y.Wang,J.Zhang,L.Wang,C.Fan,T.K.Lu,C.Zhong*,ProgrammableandPrintableBacillussubtilisBiofilmsasEngineeredLivingMaterials.NatureChemicalBiology2019,15,34–41.
X.Wang#,J.Pu#,B.An#,Y.Li,Y.Shang,Z.Ning,Y.Liu,F.Ba,J.Zhang,C.Zhong*,Programmingcellsfordynamicassemblyofinorganicnano-objectswithspatiotemporalcontrol.AdvancedMaterials2018,30,1705968.(Insidebackcover&videoabstracthighlight)
C.Zhang#,Y.Li#,H.Wang,S.He,Y.Xu,C.Zhong*andT.Li*,Adhesivebacterialamyloidnanofiber-mediatedgrowthofmetal–organicframeworksondiversepolymericsubstrates.ChemicalScience2018,9,5672–5678.
Y.Wang#,J.Hua#,B.An#,T.K.Lu*,C.Zhong*,EmergingParadigmsforSyntheticDesignofFunctionalAmyloids,JournalofMolecularBiology2018inpress,(Invitedreviewinthemeissue“functionalamyloids”).
L.Jiang,X.Song,Y.Li,Q.Xu,J.Pu,H.Huang*,andC.Zhong*,Programmingintegrativeextracellularandintracellularbiocatalysisforrapid,robust,andrecyclablesynthesisoftrehalose.ACSCatalysis2018,8,1837−1842.
Q.Qi,T.Zhao,B.An,X.Liu,C.Zhong*,Self-assemblyandmorphologicalcharacterizationoftwo-componentfunctionalamyloidproteins,ChineseChemicalLetters,2017,28(5),1062-1068.DOI:10.1016/j.cclet.2016.12.008
B.An#,X.Wang#,M.Cui#,X.Gui,X.Mao,Y.Liu,K.Li,C.Chu,J.Pu,S.Ren,Y.Wang,G.Zhong,T.K.Lu,C.Liu,andC.Zhong*,Diversesupramolecularnanofibernetworksassembledbyfunctionallow-complexitydomains.ACSNano2017,11(7),6985–6995.
M.Cui#,S.Ren#,S.Wei#,C.Sun,C.Zhong*,Naturalandbio-inspiredunderwateradhesives:currentprogressandnewperspectives.APLMaterials2017,5,116102.(Invitedreview).
X.Y.Wang#,Y.F.Li#,C.Zhong*,Amyloid-directedassemblyofnanostructuresandfunctionaldevicesforbionanoelectronics.JournalofMaterialsChemistryB.2015,3,4953-4958.(Invitedreview).
BeforeJoiningShanghaiTech
C.Zhong,T.K.Luetal,Strongunderwateradhesivesmadebyself-assemblingmulti-proteinnanofibres.NatureNanotechnology2014,10,858-866.(HighlightedinMITNews,theScientist,ScienceDaily,MaterialsToday,MRSwebsiteandothers)
C.Zhong,Y.X.Deng,A.Kapetanovic,M.Rolandi,Apolysaccharidebioprotonicfield-effecttransistor.NatureCommunications2011,2,476.(HighlightedbyMITTechReview,IEEESpectrum,MRSwebsiteandothers).
C.Zhong,A.Kapetanovic,Y.X.Deng,M.Rolandi,Achitinnanofiberinkforairbrushing,replicamoldingandmicrocontactprintingofself-assembledmarco-,micro-andnanostructures.AdvancedMaterials2011,23,4776-4781.(Insidecoverfeature).
C.Zhong,C.C.Chu,Biomimeticmineralizationofacidpolysaccharide-basedhydrogels:Towardsporous3-dimensionalbone-likebiocomposites.JournalofMaterialsChemistry2012,22,6080-6087.(HighlightedinBiomimeticMaterialsCollectiononRSCBiomaterialsScienceBlog).
C.Zhong,C.C.Chuetal.,Synthesis,characterizationandcytotoxicityofphoto-crosslinkedmaleicchitosan-PEGDAhybridhydrogels.ActaBiomaterialia,2010,6,3908-3918.
C.Zhong,A.Cooper,A.Kapetanovic,M.Zhang,M.Rolandi,Afacilebottom-uproutetoself-assembledbiogenicchitinnanofibers.SoftMatter2010,6,5298-5301.
C.Zhong,C.C.Chu,Ontheoriginofamorphouscoresinbiomimeticcalciumcarbonatespherulites:newinsightsintospheruliticcrystallization.CrystalGrowthandDesign2010,10,5043-5049.
C.Zhong,C.C.Chu,Acidpolysaccharide-inducedamorphouscalciumcarbonate(ACC)films:colloidalnanoparticleself-organizationprocess.Langmuir2009,25,3045–3049.
A.Y.Chen#,C.Zhong#,T.K.Lu,Engineeringlivingfunctionalmaterials.ACSSyntheticBiology2015,4,8-11(InvitedPerspectivepaper).
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