个人简介

Ph.D., Stanford University, 1974 Postdoctoral Associate, University of Illinois, Urbana IL, 1974-1975 Postdoctoral Associate, University of California San Diego, 1975-1978

研究领域

Chemical Biology and Biochemistry

My group has two major research areas: (i) defining on a molecular level how interfaces modulate lipolytic enzyme activities, and how this can affect signal transduction pathways, and (ii) understanding stress responses in various cells and microorganisms, in particular the range, biosynthesis, and regulation of novel solutes in extremophiles. NMR spectroscopic techniques (with an emphasis on 31P for phospholipase assays and substrate characterization, indirect detection of 13C or 15N uptake and turnover in archaea and some mammalian cells, and of late, the novel method of high resolution NMR field cycling to monitor lipid dynamics and conformations) are the primary tools for these studies.

近期论文

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C.A. Doughty, B.F. Bleiman, D.J. Wagner, F.J. Dufort, J.M. Mataraza, M.F. Roberts, and T.C. Chiles, “Receptor-mediated changes in glucose metabolism in B lymphocytes: role of phosphatidylinositol-3-kinase signaling in the glycolytic control of growth,” Blood 107, 4458-4465 (2006). A.C. Faber, F.J. Dufort, D. Blair, D. Wagner, M.F. Roberts, and T.C. Chiles, “Inhibition of phosphatidylinositol 3-kinase-mediated glucose metabolism coincides with resveratrol-induced cell cycle arrest in human diffuse large B-cell lymphomas,” Biochem. Biopharm. 29, 1561-1566 (2006). C. Shao, X. Shi, H. Wehbi, C. Zambonelli, J.F. Head, B.A. Seaton, and M.F. Roberts, “Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C,” J. Biol. Chem., 282, 9228-9235 (2007). D.A. Rodionov, O.V. Kurnasov, B. Stec, Y. Wang, M.F. Roberts, and A. Osterman, “Genomic identification and in vitro reconstitution of a complete biosynthetic pathway for the osmolyte di-myo-inositol phosphate,” Proc. Natl. Acad. Sci. U.S.A. 104, 4279-4284 (2007). E. Crouch, B. McDonald, K Smith, M. Roberts, T. Cafarella, B. Seaton, and J. Head, “Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein D,” Biochemistry 46, 5160-5169 (2007). K.A. Stieglitz, M.F. Roberts, W. Li, and B. Stec, “Crystal structure of the tetrameric IMPase (TM1415) from the hyperthermophile Thermotoga maritima,” FEBS J. 274, 2461-2469 (2007). Y.K. Wang, K.A. Stieglitz, M. Bubunenko, D.L. Court, B. Stec, and M.F. Roberts, “The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications its functional interactions in E. coli,” J. Biol. Chem. 282, 26989-26996 (2007). B.F. Bleiman, F.J. Dufort, M.R. Gumina, D. Blair, D.J. Wagner, M.F. Roberts, and T.C. Chiles, “Interleukin-4-mediated protection of primary B lymphocytes from apoptosis via Stat6-dependent regulation of glycolytic metabolism,” J. Immunol. 179, 4953-4957 (2007). J.B. Klauda, M.F. Roberts, A.G. Redfield, B.R. Brooks, and R.W. Pastor, “Rotation of lipids in membranes: MD simulation, 31P spin-lattice relaxation, and rigid-body dynamics,” Biophys. J. 94, 3074-3083 (2008). S. Guo, X. Zhang, B. A. Seaton, and M. F. Roberts, “Role of helix B residues in interfacial activation of a bacterial PI-PLC,” Biochemistry 47, 4201-4210 (2008). Y.K. Wang, W. Chen, D. Blair, M. Pu, Y. Xu, S.J. Miller, A.G. Redfield, T.C. Chiles, and M. F. Roberts, “Insights into the structural specificity of the cytotoxicity of 3-deoxy-phosphatidylinositols,” J. Am. Chem. Soc. 130, 7746-7755 (2008). V.N. Sivanandam, J. Cai, A.G. Redfield, and M.F. Roberts, “Phosphatidylcholine “wobble” in vesicles assessed by high-resolution 13C field cycling NMR spectroscopy,” J. Am. Chem. Soc. 131, 3420-3421 (2009). W. Chen, H. Goldfine, B. Ananthanarayanan, W. Cho, and M.F. Roberts, “Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Kinetic activation and homing in on different interfaces,” Biochemistry 48, 3578-3592 (2009). M.F. Roberts, U. Mohanty, and A.G. Redfield, “Phospholipid reorientation at the lipid/water interface measured by high resolution field cycling 31P NMR spectroscopy,” Biophys. J. 97, 132-141 (2009). C.M. Longo, Y. Wei, M.F. Roberts, and S.J. Miller, “Asymmetric syntheses of L,L- and L,D-di-myo-inositol-1,1’-phosphate and heir behavior as stabilizers of enzyme activity at extreme temperatures, Angew. Chem. Int. Ed. 48, 4158-4161 (2009). X. Shi, C. Shao, X. Zhang, C. Zambonelli, A.G. Redfield, J.F. Head, B.A. Seaton, and M.F. Roberts, “Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface,” J. Biol. Chem. 284, 15607-15618 (2009). M. Pu, X. Fang, A. Gershenson, A.G. Redfield, and M.F. Roberts, “Correlation of vesicle binding and phospholipid dynamics with phospholipase C activity: Insights into phosphatidylcholine activation and surface dilution inhibition,” J. Biol. Chem. 284, 16099-16107 (2009). M. Pu, M.F. Roberts, and A. Gershenson, “Fluorescence correlation spectroscopy of phosphatidylinositol-specific phospholipase C monitors the interplay of substrate and activator lipid binding,” Biochemistry 48, 6835-6845 (2009). M. Pu, J. Feng, A.G. Redfield, and M.F. Roberts, “Enzymology with a spin-labeled phospholipase C: soluble substrate binding by 31P NMR from 0.005 to 11.7 Tesla,” Biochemistry 48, 8282-88284 (2009). Z. Li, K.A. Stieglitz, A.L. Shrout, Y. Wei, R.M. Weis, B. Stec, and M.F. Roberts, “Mobile loop mutations in an archaeal inositol monophosphatase: modulating three-metal ion assisted catalysis and lithium inhibition,” Prot. Sci. 19, 309-318 (2009).