李强 - 清华大学 - 化学工程系

个人简介

主要从事生物化工方面的研究工作，先后涉及到了包括: 酶催化动力学、酶的固定化及相关酶反应器研究；工业微生物的发酵动力学、发酵工艺优化及新型发酵设备的设计研究；植物细胞培养及优化；杂交瘤细胞大规模培养制备单抗及体外免疫检测试剂盒的研制；基因工程和生物催化等研究领域。在生物化工领域曾发表论文60余篇, 先后完成了“两步酶法催化生产医药中间体7－ACA”工业化，填补了国家空白；“一菌双酶生产医药中间体D-对羟基苯甘氨酸Sino菌的基因工程改造”也实现了工业化，是目前工业应用性能最好，最稳定的菌株；“酶法催化从果糖制备D-甘露糖”技术也已经完成了中试，并生产出了合格的D-甘露糖产品，是目前报道的第一个实现酶法生产D-甘露糖技术。教育与工作经历 1985 清华大学化学与化学工程系工学学士 1988 清华大学化学工程系工学硕士 1997-1999 东京大学工学院访问学者 1988- 1995 清华大学化学工程系讲师 1995-2001 清华大学化学工程系副教授生化所副所长，实验室主任 2001-2004 清华大学化学工程系副教授，系党委副书记 2004- 至今清华大学化学工程系副教授，博士生导师

研究领域

近年来一直从事基因工程和生物催化相关课题研究，包括：高活性表达催化用酶工程菌的构建，以提高菌株品质为目标的工业菌株的基因改造，T7系统稳定性研究及通用表达平台的构建等。这些技术主要用于工业菌株改造，针对关系到国计民生的医药工业用酶的高效、低成本制备，致力于研发以生物技术为基础的绿色环保工艺。

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Xiao Liang, Chenmeng Li, Wenya Wang, Qiang Li. Integrating T7 RNA polymerase and its cognate transcriptional units for a host-independent and stable expression system in single plasmid. ACS Synthetic Biology, 2018, 7(5): 1424-1435. Wang, W., Li, Y., Wang, Y., Shi, C., Li, C., Li, Q., Linhardt, R. J. Bacteriophage T7 transcription system: an enabling tool in synthetic biology. Biotechnology Advances. 2018,36(8) : 2129-2137. Gang Wang, Qiang Li*, Dikai Xu, Mingxin Cui, Xiao Sun, Yanyan Xu and Wenya Wang. Construction of a host-independent T7 expression system with small RNA regulation. Journal of Biotechnology, 2014.189(10): 72-75. Jixian Hou, Quan Jin, Jing Du, Qiang Li, Qipeng Yuan, Jichu Yang. A rapid in situ immobilization of D-Amino acid oxidase based on immobilized metal affinity chromatography. Bioprocess Biosyst Eng. 2014,37(5):857-64. Jixian Hou，Yangqiu Liu，Qiang Li，Jichu Yang. High activity expression of D-amino acid oxidase in Escherichia coli by the protein expression rate optimization. Protein expression and purification, 2013, 88(1):120-126. Xi Xiong, Qiang Li, Xu-Cheng Zhang, Li Wang, Zhao-Xia Guo, Jian Yu. Poly(vinylidene fluoride)/Silica Nanocomposite Membranes by Electrospinning. Journal of Applied Polymer Science. 2013,129(3): 1089–1095. .Xi Xiong, Qiang Li, Jian-Wei Lu, Zhao-Xia Guo, Zhao-Hui Sun and Jian Yu，Fibrous Scaffolds Made by Co-Electrospinning Soluble Eggshell Membrane Protein with Biodegradable Synthetic Polymers. Journal of Biomaterials Science, Polymer Edition. 2012,23(9:1217-1230. Xi Xiong, Qiang Li, Xu-Cheng Zhang, Li Wang, Zhao-Xia Guo, Jian Yu. Poly(vinylidene fluoride)/Silica Nanocomposite Membranes by Electrospinning. Journal of Applied Polymer Science. 2012, DOI: 10.1002/APP.38787 Xi Xiong, Qiang Li, Jian-Wei Lu, Zhao-Xia Guo, Jian Yu. Poly(lactic acid)/soluble eggshell membrane protein blend films: Preparation and characterization. Journal of Applied Polymer Science. 2010,117(4), : 1955-1959. Yangqiu Liu, Qiang Li, Hongyu Zhu & Jichu Yang，High soluble expression of D-amino acid oxidase in 1，Escherichia coli regulated by a native promoter. Applied Biochemistry and Biotechnology, 2009,158(2): 313-322. Yangqiu Liu, Qiang Li, Xiaojia Hu & Jichu Yang，Using native hydantoinase promoter to induce D-carbamoylase soluble expression in Escherichia coli. Biochemical Engineering : 2008,41(1):12-16. Yangqiu Liu, Qiang Li, Xiaojia Hu & Jichu Yang，Using Construction and Co-expression of Polycistronic Plasmid Encoding D-hydantoinase and D-carbamoylase for the Production of D-amino Acids. Enzyme and Microbial Technology: 2008, 42(7):589-593.