个人简介

PhD | Biology | California Inst of Tech 1999 BS | Molecular Biology | University of Wisconsin-Madison 1994

研究领域

Structural and Biophysical Basis of Normal and Disease-Associated pre-mRNA Recognition

Up to half of human genetic diseases, including cancers and neuromuscular disorders, are associated with defects in splicing of pre-mRNA transcripts. Almost all human genes contain intervening, non-coding sequences that must be spliced from the pre-mRNA transcripts before translation into proteins. The majority of these are alternatively spliced to encode a number of protein variants depending on different cellular needs. My laboratory uses structural and biophysical techniques to understand how pre-mRNA splice sites are recognized in normal cells, and to suggest possible means for treating defective pre-mRNA splicing observed in human genetic diseases.

近期论文

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2013 Apr 1 Jenkins JL, Agrawal AA, Gupta A, Green MR, Kielkopf CL. "U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs." Nucleic acids research. 2013 Apr 1; 41(6):3859-73. Epub 2013 Feb 01. Apr Gleghorn ML, Gong C, Kielkopf CL, Maquat LE. "Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay." Nature structural & molecular biology. 2013 Apr; 20(4):515-24. Epub 2013 Mar 24. 2012 Jul 3 Jenkins JL, Laird KM, Kielkopf CL. "A Broad range of conformations contribute to the solution ensemble of the essential splicing factor U2AF(65)." Biochemistry. 2012 Jul 3; 51(26):5223-5. Epub 2012 Jun 19. 2012 Jan 27 Bauer WJ, Heath J, Jenkins JL, Kielkopf CL. "Three RNA recognition motifs participate in RNA recognition and structural organization by the pro-apoptotic factor TIA-1." Journal of molecular biology. 2012 Jan 27; 415(4):727-40. Epub 2011 Dec 02. 2011 Apr 1 Gupta A, Kielkopf CL. "Purification, crystallization and preliminary X-ray crystallographic analysis of a central domain of human splicing factor 1." Acta crystallographica. Section F, Structural biology and crystallization communications. 2011 Apr 1; 67(Pt 4):486-90. Epub 2011 Mar 25.