李文辉 - 北京生命科学研究所 -

个人简介

李文辉博士北京生命科学研究所资深研究员教育经历 Education 1994兰州大学医学院医学学士学位 Lanzhou University, School of Medicine Bachelor Degree of Medicine 1997兰州生物制品研究所免疫学硕士学位 Lanzhou Institute of Biological Products M.S. in Immunology 2001中国协和医科大学中国医学科学院病原生物学博士学位 Peking Union Medical College & Chinese Academy of Medical Sciences Ph.D. in Pathogens Biology 工作经历 Professional Experience 2015.3-北京生命科学研究所资深研究员 Investigator, National Institute of Biological Sciences, Beijing 2007.10-2015.2北京生命科学研究所研究员 Assistant Investigator, National Institute of Biological Sciences, Beijing 2004.6-2007.9哈佛大学医学院 Instructor Harvard Medical School, Boston 2001.9-2004.5哈佛大学医学院博士后 Postdoctoral Fellow, Harvard Medical School, Boston 研究概述 Research Description 本实验室的研究兴趣集中于重要病毒感染的分子机制及其防治。近年来，我们主要致力于乙肝病毒（HBV）研究。乙型肝炎是危害人类健康的严重传染病。全球估计有HBV感染者2.4亿人，其中我国约有9300万人。慢性HBV感染是导致肝硬化、肝癌的重要病因。丁型肝炎病毒（HDV）是HBV的卫星病毒,在所有HBV感染者中，约有1500万人同时感染HDV。我们发现HBV及HDV感染肝细胞的关键受体是钠离子-牛磺胆酸共转运蛋白（NTCP）；稳定表达人NTCP的HepG2细胞系(HepG2-NTCP)已成为HBV相关基础病毒学研究和抗病毒药物研发的重要平台。目前，对乙肝感染的科学研究及药物开发已进入一个充满生机的新时代。本实验室综合运用病毒学，生物化学，免疫学，化学生物学等多学科方法深入剖析HBV及HDV感染过程的分子基础，以帮助理解其病理机制。我们也希望研发新的抗病毒药物，为最终有效解除患者的病痛而努力。此外，我们也与相关实验室合作研究NTCP/胆酸等分子在感染及机体代谢过程中的相关作用。

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

1.He, W.,B.Ren, F.Mao,Z.Jing,Y.Li,Y.Liu,B.Peng, H. Yan,Y.Qi,Y.Sun, J-T.Guo,J.Sui,F.Wang, andW.Li*. Hepatitis D Virus Infection of Mice Expressing Human Sodium Taurocholate Co-transporting Polypeptide.PLOS Pathog, 10.1371/journal.ppat.1004840, 2015. 2.Yan, H., B. Peng, Y. Liu, G. Xu, W. He, B. Ren, Z. Jing, J. Sui, and W. Li*. Viral entry of hepatitis B and d viruses and bile salts transportation share common molecular determinants on sodium taurocholatecotransporting polypeptide. Journal of virology, 88(6): p. 3273-84, 2014. 3.Sun, Y*., Y. Qi, C. Liu, W. Gao, P. Chen, L. Fu, B. Peng, H. Wang, Z. Jing, G. Zhong, and W. Li*. Nonmuscle myosin heavy chain IIA is a critical factor contributing to the efficiency of early infection of severe fever with thrombocytopenia syndrome virus. Journal of virology, 88(1):237-48, 2014. 4.Xu, G., Z. Gao, W. He, Y. Ma, X. Feng, T. Cai, F. Lu, L. Liu, and W. Li*. microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture. Virology, 448: 247-54, 2014. 5.Zhong, G., H. Yan, H. Wang, W. He, Z. Jing, Y. Qi, L. Fu, Z. Gao, Y. Huang, G. Xu, X. Feng, J. Sui, and W. Li*. Sodium taurocholatecotransporting polypeptide mediates woolly monkey hepatitis B virus infection of Tupaia hepatocytes. Journal of virology, 87(12): 7176-84, 2013. 6.Yan, H., B. Peng, W. He, G. Zhong, Y. Qi, B. Ren, Z. Gao, Z. Jing, M. Song, G. Xu, J. Sui, and W. Li*. Molecular determinants of hepatitis B and D virus entry restriction in mouse sodium taurocholatecotransporting polypeptide. Journal of virology, 87(14): 7977-91, 2013. 7.Liu, F., M. Campagna, Y. Qi, X. Zhao, F. Guo, C. Xu, S. Li, W. Li, T.M. Block, J. Chang, and J.T. Guo*. Alpha-interferon suppresses hepadnavirus transcription by altering epigenetic modification of cccDNAminichromosomes.PLoSPathog, 9(9): p. e1003613, 2013. 8.Lin, S., H. Yan, L. Li, M. Yang, B. Peng, S. Chen, W. Li*, and P.R. Chen*. Site-Specific Engineering of Chemical Functionalities on the Surface of Live Hepatitis D Virus. AngewandteChemie International Edition, 52(52): 13970-13974, 2013. 9.Jemielity, S., J.J. Wang, Y.K. Chan, A.A. Ahmed, W. Li, S. Monahan, X. Bu, M. Farzan, G.J. Freeman, D.T. Umetsu, R.H. Dekruyff, and H. Choe*. TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine. PLoSPathog, 9(3): e1003232, 2013. 10.Yan, H., G. Zhong, G. Xu, W. He, Z. Jing, Z. Gao, Y. Huang, Y. Qi, B. Peng, H. Wang, L. Fu, M. Song, P. Chen, W. Gao, B. Ren, Y. Sun, T. Cai, X. Feng, J. Sui, and W. Li*. Sodium taurocholatecotransporting polypeptide is a functional receptor for human hepatitis B and D virus.eLife, 1: e00049, 2012. 11.Chen, P., Z. Song, Y. Qi, X. Feng, N. Xu, Y. Sun, X. Wu, X. Yao, Q. Mao, X. Li, W. Dong, X. Wan, N. Huang, X. Shen, Z. Liang, and W. Li*. Molecular determinants of enterovirus 71 viral entry: cleft around GLN-172 on VP1 protein interacts with variable region on scavenge receptor B 2. J BiolChem, 287(9): 6406-20, 2012. 12.Huang, Z., Y. Feng, D. Chen, X. Wu, S. Huang, X. Wang, X. Xiao, W. Li, N. Huang, L. Gu, G. Zhong, and J. Chai*. Structural basis for activation and inhibition of the secreted chlamydia protease CPAF. Cell Host Microbe, 4(6):529-42, 2008. 13.Huang, I.-C., W. Li, J. Sui, W. Marasco, H. Choe, and M. Farzan*. Influenza A virus neuraminidase limits viral superinfection. Journal of virology, 82(10): 4834-4843, 2008. 14.Radoshitzky, S.R., J. Abraham, C.F. Spiropoulou, J.H. Kuhn, D. Nguyen, W. Li, J. Nagel, P.J. Schmidt, J.H. Nunberg, N.C. Andrews, M. Farzan, and H. Choe*. Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses. Nature, 446(7131): p. 92-6, 2007. 15.Li, W., J. Sui, I. Huang, J.H. Kuhn, S.R. Radoshitzky, W.A. Marasco, H. Choe, and M. Farzan*. The S proteins of human coronavirus NL63 and severe acute respiratory syndrome coronavirus bind overlapping regions of ACE2. Virology, 367(2): 367-374, 2007. 16.Zhang, L., F. Zhang, W. Yu, T. He, J. Yu, C.E. Yi, L. Ba, W. Li, M. Farzan, Z. Chen, K.Y. Yuen, and D. Ho*. Antibody responses against SARS coronavirus are correlated with disease outcome of infected individuals. J Med Virol, 78(1): 1-8, 2006. 17.Li, F., M. Berardi, W. Li, M. Farzan, P.R. Dormitzer, and S.C. Harrison*. Conformational states of the severe acute respiratory syndrome coronavirus spike protein ectodomain. Journal of virology, 80(14): 6794-6800, 2006. 18.Kuhn, J.H., S.R. Radoshitzky, A.C. Guth, K.L. Warfield, W. Li, M.J. Vincent, J.S. Towner, S.T. Nichol, S. Bavari, H. Choe, M.J. Aman, and M. Farzan*. Conserved receptor-binding domains of Lake Victoria marburgvirus and Zaire ebolavirus bind a common receptor. J BiolChem, 2006. 281(23): 15951-8. 19.Huang, I.-C., B.J. Bosch, F. Li, W. Li, K.H. Lee, S. Ghiran, N. Vasilieva, T.S. Dermody, S.C. Harrison, and P.R. Dormitzer*. SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells. J BiolChem, 281(6): 3198-3203, 2006. 20.He, Y., J. Li, W. Li, S. Lustigman, M. Farzan, and S. Jiang*. Cross-neutralization of human and palm civet severe acute respiratory syndrome coronaviruses by antibodies targeting the receptor-binding domain of spike protein. Journal of Immunology, 176(10): 6085-6092, 2006. 21.Sui, J., W. Li, A. Roberts, L.J. Matthews, A. Murakami, L. Vogel, S.K. Wong, K. Subbarao, M. Farzan, and W.A. Marasco*. Evaluation of human monoclonal antibody 80R for immunoprophylaxis of severe acute respiratory syndrome by an animal study, epitope mapping, and analysis of spike variants. Journal of virology, 79(10): 5900-5906, 2005. 22.Li, W., C. Zhang, J. Sui, J.H. Kuhn, M.J. Moore, S. Luo, S.K. Wong, I.C. Huang, K. Xu, N. Vasilieva, A. Murakami, Y. He, W.A. Marasco, Y. Guan, H*. Choe*, and M. Farzan*. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. EMBO J, 24(8): 1634-43, 2005. 23.Li, F., W. Li, M. Farzan, and S.C. Harrison*. Structure of SARS coronavirus spike receptor-binding domain complexed with receptor. Science, 309(5742): 1864-1868, 2005. 24.Choe, H., M.J. Moore, C.M. Owens, P.L. Wright, N. Vasilieva, W. Li, A.P. Singh, R. Shakri, C.E. Chitnis, and M. Farzan*. Sulphatedtyrosines mediate association of chemokines and Plasmodium vivax Duffy binding protein with the Duffy antigen/receptor for chemokines (DARC). Molecular microbiology, 55(5): 1413-1422, 2005. 25.Wong, S.K., W. Li, M.J. Moore, H. Choe, and M. Farzan*. A 193-amino acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 2. J Biol Chem,279(5): 3197-3201, 2004. 26.Sui, J., W. Li, A. Murakami, A. Tamin, L.J. Matthews, S.K. Wong, M.J. Moore, A.S. Tallarico, M. Olurinde, H. Choe, L.J. Anderson, W.J. Bellini, M. Farzan, and W.A. Marasco*. Potent neutralization of severe acute respiratory syndrome (SARS) coronavirus by a human mAb to S1 protein that blocks receptor association. Proc Natl AcadSci U S A, 101(8): 2536-41, 2004. 27.Moore, M.J., T. Dorfman, W. Li, S.K. Wong, Y. Li, J.H. Kuhn, J. Coderre, N. Vasilieva, Z. Han, T.C. Greenough, M. Farzan, and H. Choe*. Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2. Journal of virology, 78(19):10628-35, 2004. 28.Li, W., T.C. Greenough, M.J. Moore, N. Vasilieva, M. Somasundaran, J.L. Sullivan, M. Farzan*, and H. Choe*. Efficient replication of severe acute respiratory syndrome coronavirus in mouse cells is limited by murine angiotensin-converting enzyme 2. Journal of virology, 78(20):11429-11433, 2004. 29.He, Y., Y. Zhou, S. Liu, Z. Kou, W. Li, M. Farzan, and S. Jiang*. Receptor-binding domain of SARS-CoV spike protein induces highly potent neutralizing antibodies: implication for developing subunit vaccine. Biochemical and biophysical research communications, 324(2): 773-781, 2004. 30.Li, W., M.J. Moore, N. Vasilieva, J. Sui, S.K. Wong, M.A. Berne, M. Somasundaran, J.L. Sullivan, K. Luzuriaga, T.C. Greenough, H. Choe*, and M. Farzan*. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965): 450-454, 2003. 31.Choe, H., W. Li, P.L. Wright, N. Vasilieva, M. Venturi, C.C. Huang, C. Grundner, T. Dorfman, M.B. Zwick, L. Wang, E.S. Rosenberg, P.D. Kwong, D.R. Burton, J.E. Robinson, J.G. Sodroski, and M. Farzan*. Tyrosine sulfation of human antibodies contributes to recognition of the CCR5 binding region of HIV-1 gp120. Cell, 114(2): 161-170, 2003. 32.Farzan, M., S. Chung, W. Li, N. Vasilieva, P.L. Wright, C.E. Schnitzler, R.J. Marchione, C. Gerard, N.P. Gerard, and J. Sodroski*. Tyrosine-sulfated peptides functionally reconstitute a CCR5 variant lacking a critical amino-terminal region. J BiolChem, 277(43):40397-40402, 2002. 33.Li, W.,Y. Zhang,J.Sui, S. Wang *. Combined immunization of DNA vaccine and replication-defective recombinant adenovirus bearing rabies glycoprotein gene induces immune response against rabies virus. Chin J Micro and Immuno, 22(4): 403-406,2002 (in Chinese) 34.Sui, J.,W.Li, X.Jiang, Y. He,Z. Song*. Highly Efficient Expression and Functional Studies of An Anti-KG1a Cell scFv 5C1 derived from Phage Display Antibody Library. Chin J Micro and Immuno, 21(4) : 437~441,2001 (in Chinese) 35.Li, W., Y. Zhang, S. Wang*, L. Liu. Immune response of mice against replication-defective recombinant adenovirus containing glycoprotein gene of rabies 3aG strain. Chin J Exp and Clin Viro,15(1):35-39,2001 (in Chinese) 36.Li, W., S. Wang*, Y. Zhang, L.Liu. Construction of cloned recombinant adenovirus genome by homologous recombination in Escherichia coli. Chin JBiochem and Mole Bio, 16(3):346-35,2000 (in Chinese) 37.Zhang, X., Y. Zhang, W. Li, S. Wang*.Expression of glycoprotein gene of the rabies virus 3aG strain by E-3 deleted adenovirus recombinant. Chin J of Exp and ClinViro, 14 (3).281-284, 2000 (in Chinese) 受邀综述 Invited Review Articles: 1.Li, W. Hepatitis B virus receptor, Annual Review of Cell and Developmental Biology,doi/abs/10.1146, 2015. 2.Yan, H and W.Li*. NTCP acts as a receptor for hepatitis B and D virus,Digestive DiseasesClinical Reviews,doi:10.1159/000371692,2015. 3.Watashi*, K., S. Urban, W. Li, and T. Wakita. NTCP and Beyond: Opening the Door to Unveil Hepatitis B Virus Entry. Int J MolSci, 15(2): p.2892-905, 2014. 4.Li, W*, S.-K. Wong, F. Li, J.H. Kuhn, I.-C. Huang, H. Choe, and M. Farzan*. Animal origins of the severe acute respiratory syndrome coronavirus: insight from ACE2-S-protein interactions. Journal of virology, 80(9): 4211-4219, 2006. 5.Kuhn, J., W. Li, H. Choe, and M. Farzan*. Angiotensin-converting enzyme 2: a functional receptor for SARS coronavirus. Cellular and molecular life sciences: 61(21): 2738-2743, 2004.