马立新 - 湖北大学 - 生命科学学院

个人简介

1985年9月-1989年7月，中国农业大学动物遗传与育种专业，获农学学士学位 1991年9月-1994年7月，复旦大学遗传学专业，获理学硕士学位 1994年9月-1997年7月，华中农业大学微生物学专业，获理学博士学位 2010年10月-2011年10月，哈佛大学波士顿儿童医学院访问学者 1997年-今，湖北大学生命科学学院讲师、教授

研究领域

基因工程、蛋白质工程、工业酶表达及其工艺研究

近期论文

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Hu F, Li X, Lü J, Mao PH, Jin X, Rao B, Zheng P, Zhou YL, Liu SY, Ke T, Ma XD, Ma LX* A visual method for direct selection of high-producing Pichia pastoris clones. BMC Biotechnology, 2011 Mar 21:11:23. Ke T, Ma XD, Mao PH, Jin X, Chen SJ, Li Y, Ma LX, He GY A mutant alpha-amylase with only part of the catalytic domain and its structural implication. Biotechnol Lett, 2007 Jan:29(1):117-22. Li J, Li C, Xiao W, Yuan D, Wan G, Ma L*. Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli. Analytical Biochemistry, 2008 Feb 15:373(2):389-91. Yang XS, Jiang ZB, Song HT, Jiang SJ, Madzak C, Ma LX* Cell-surface display of the active mannanase in Yarrowia lipolytica with a novel surface-display system. Biotechnology And Applied Biochemistr, 2009 Oct 13:54(3):171-6. Yang J, Xu J, Zhong X, Zhang Y, Wu Q, Li W, Zhang X, Zhen P, Yang X, Chen L, Wang Y, Zhu D, Zhai C, Ma L* An advanced blue-white screening method for construction of shRNA expression vectors. Biochemical And Biophysical Research Communications, 2009，390（1） : 97-102. Jiang S, Zhong X, Zhai C, Chen L, Ma L, Jin M, Chen H. Constructing recombinant herpesvirus BAC vectors with mating-assisted genetically integrated clone method. Biotechnology Letters, 2010，32（7）: 903-907. Hu F, Ke T, Li X, Mao PH, Jin X, Hui FL, Ma XD, Ma LX*Expression and Purification of an Antimicrobial Peptide by Fusion with Elastin-like Polypeptides in Escherichia coli. Applied Biochemistry And Biotechnology, 2010，160（8）: 2377-2387. Wu Q, Zhong X, Zhai C, Yang J, Chen X, Chen L, Wang W, Ma L* A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products. Biotechnology Letters, 2010，32 （3）: 439-443. Zhu D, Zhong X, Tan R, Chen L, Huang G, Li J, Sun X, Xu L, Chen J, Ou Y, Zhang T, Yuan D, Zhang Z, Shu W, Ma L* High-throughput cloning of human liver complete open reading frames using homologous recombination in Escherichia coli. Analytical Biochemistry, 2010，397（2）: 162-167. Yan H, Zhong X, Jiang S, Zhai C, Ma L* Improved method for constructing plant amiRNA vectors with blue-white screening and MAGIC. Biotechnology Letters, 2011,33(8): 1683-1688. Li C, Ma L, Chen X Interferon regulatory factor 3-CL, an isoform of IRF3, antagonizes activity of IRF3. Cellular & Molecular Immunology, 2011,8(1): 67-74. Chen X, Zhai C, Kang L, Li C, Yan H, Zhou Y, Yu X, Ma L* High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris. Biotechnology Letter, 2012 Apr; 34(4):689-94. Zhong X, Zhai C, Yang D, Jiang S, Li Z, Yu X, Chen L, Zhang Z, Wang F, Wang Y, Chen W, Ma L* A single-step mixing cloning method for assembly of lentiviral shRNA expression vectors for gene silencing. Analytical Biochemistry, 2013.03.011. Gui-Min Zhang1,2, Yong Hu2, Yong-Hong Zhuang2, Li-Xin Ma2† and Xian-En Zhang Molecular cloning and heterologous expression of an alkaline xylanase from Bacillus pumilus HBP8 in Pichia pastoris. Biocatalysis and Biotransformation, 2006，24(5):371 - 379. Song HT, Jiang ZB, Ma LX * Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216. Protein expression and purification, 2006 Jun;47(2):393-397. Jiang ZB, Song HT, Gupta N, Ma LX, Wu ZB Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris. Protein Expr Purif, 2007 Nov;56(1):35-9. Ma X, Ke T, Mao P, Jin X, Ma L, He G The mutagenic properties of BrdUTP in a random mutagenesis process. Microbiology And Molecular Biology Reviews, 2008 Dec;35(4):663-7. Rao B, Zhong X, Wang Y, Wu Q, Jiang Z, Ma L* Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris. Yeast, 2010，27（5）: 285-292. Kang L, Chen X, Zhai C, Ma L* Synthesis and high expression of chitin deacetylase from Colletotrichun lindemuthianum in Pichia pastoris GS115. Journal of Microbiology and Biotechnology, 2012,22(9):1202-1207. Kang LX, Chen XM, Fu L, Ma LX* Recombinant expression of chitosanase from Bacillus subtilis HD145 in Pichia pastoris. Carbohydrate Research, 2012(352):37-43.