个人简介

2013-present, Assistant Professor, Dept. of Chemistry, Washington University in St. Louis

研究领域

Magnetic Resonance/Dynamic Nuclear Polarization/Structural Biology/Rational Drug Design/HIV Eradication/Alzheimers/Cancer/Electrical Engineering/Gyrotron Technology/Molecular Biology/Biophysical Chemistry

Our laboratory applies improvements in magnetic resonance spectroscopy to biomolecular structure determination. Solid state nuclear magnetic resonance (NMR) is exquisitely suited to probe atomic level structural detail of biomolecules not amendable to x-ray crystallography. However, the small nuclear magnetic moments that yield narrow resonances also result in very low inherent sensitivity in NMR. Dynamic Nuclear Polarization (DNP) is a powerful combined electron paramagnetic resonance (EPR) and NMR technique that transfers the strong polarization from unpaired electron spins to nuclear spins to boost NMR sensitivity. In our DNP experiments, we endeavor to employ frequency agile gyrotrons, and extreme cryogenic sample cooling to increase the sensitivity of solid state NMR experiments up to a factor of 20,000. This tremendous gain in sensitivity will result in acquiring data 400 million times faster than conventional NMR experiments and will have a profound impact on magnetic resonance spectroscopy and structural biology. The drastic gain in sensitivity will permit biomolecular structure determination in complex heterogeneous systems in vitro with less than a milligram of sample and in hours of experimental time, or directly in vivo in a cellular context without protein purification.

近期论文

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Barnes et al. High-Field Dynamic Nuclear Polarization for Solid and Solution Biological NMR. Appl. Mag. Reson. 34 (3), 237-263. (2008) Barnes et al. A 250 GHz Gyrotron with a 3 GHz Tuning Bandwidth For Dynamic Nuclear Polarization. J. Mag. Res. 221, 147-153. (2012) Barnes et al. Dynamic Nuclear Polarization at 700 MHz/460 GHz. Journal of Magnetic Resonance. 224, 1-7. (2012) Barnes et al. Cryogenic Sample Exchange NMR Probe for Magic Angle Spinning Dynamic Nuclear Polarization. J. Mag. Res. 198 (2), 261-270. (2009)