个人简介

Dr. Dalbey received his B.S. degree in chemistry from University of Washington in 1978 and his Ph.D. in Biochemistry from Washington State University in 1983. That same year he joined the laboratory of Professor William Wickner at UCLA as a NIH postdoctoral fellow. Then he moved from the West coast to the Midwest to become a member of the faculty at The Ohio State University in 1987. He was promoted to Full Professor in 1999. Professor Dalbey won a Junior Faculty Award (1989-1992) from the American Cancer Society. In 1996, he was a visiting professor at the Federal Institute of Technology in Lausanne, Switzerland working with Professor Horst Vogel. Dr. Dalbey is a member of the American Chemical Society, Federation of American Societies for Experimental Biology, Sigma Xi, American Society of Microbiology, and the honor Society of Phi Kappa Phi. He is an elected fellow of the AAAS.

研究领域

Biochemistry

Membrane Protein Assembly, Proteases, Biophysics Our lab’s central interest is the determination of how proteins are transported and inserted into membrane to obtain their proper structure. We are employing biochemical and in vivo approaches to understand membrane protein assembly. We have identified a novel protein, YidC that specializes in membrane protein topogenesis. The general relevance of this finding is underscored by the homology of YidC to the mitochondrial Oxa1, which functions in a novel pathway for insertion of inner membrane proteins from the mitochondrial matrix compartment. The goal now is to determine the substrate specificity of YidC, to determine the function of YidC in the integration and folding of multispanning membrane proteins, define the structural features of YidC and the insertion pore of the YidC dimer. Another serious interest here is the study of proteases involved in the cleavage of proteins and peptides that are transiently associated with cellular membranes. These proteases such as signal peptidase and signal peptide peptidases are crucially important for a wide range of essential biological processes. We are very interested in how the cell meets the “chemical challenge” of peptide bond hydrolysis in proteins that are shielded by non-aqueous environments. Recent graduates from the Dalbey lab hold faculty positions at the University of Pittsburg, Simon Fraser University, and Kwandong University. They hold research positions at National Institutes of Health (NIH), MIT, and Duke, and have industrial positions at Novartis, Eli Lilly, and Abbott Pharmaceuticals. Funding from the lab has come primarily from NIH and National Science Foundation (NSF) over the years.

近期论文

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Celebi, N. and Dalbey, R. E. (2008). Mechanism and hydrophobic forces driving membrane protein insertion of cytochrome bo oxidase. J. Mol. Biol., 375, 1282-1292. Xie, K., Hessa, T., Seppala, S., Rapp, M., von Heijne, G. and Dalbey, R. E. (2008), Features of transmembrane segments that promote the lateral release from the translocase into the lipid phase. Biochemistry, 46, 15153-15161. Xie, K. and Dalbey, R. E. (2008), Inserting proteins in bacterial membranes. Nature Reviews-Microbiology, 6, 234-244 Wang, P., Shim, E., Cravatt, B., Jacobsen, R., Schoeniger, J., Kim, AC., Paetzel., M. and Dalbey, R. E. (2008), Escherichia coli signal peptide peptidase A is a serine-lysine protease with a lysine recruited to the nonconserved amino-terminal domain in the S49 protease family. Biochemistry, 47, 6361-6369. Doğan Ekici, Ö., Paetzel, M. and Dalbey, R. E. (2008), Unconventional serine/threonine proteases: variations on the catalytic ser/his/asp triad configuration. Protein Science, 17, 2023-2037. Doğan Ekici, Ö, Zhu, J., Chung, IY, Paetzel, M., Dalbey, R. E., and Pei, D. (2009), Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide Library Approach. Biochemistry, 48, 5753-5759. Yuan, Y., Zweers, J. C. Zweers, van Dijl, J. M. and Dalbey, R. E. (2010), Protein transport across and into cell membranes in bacteria and archaea. Cell. Mol. Life Sci., 67, 179-199. Wang, P., Kuhn, A., and Dalbey, R. E. (2010). Global change of gene expression and cell physiology in YidC-depleted E. coli. J. Bacteriol., 192, 2193-2209. Dalbey, R. E. , Wang, P. and Kuhn, A. (2011). Assembly of Bacterial Inner Membrane Proteins. Annu Rev. Biochem., 80, 161-187. Stiegler, N., Dalbey, R. E. and Kuhn, A. (2011). M13 procoat protein insertion into YidC and SecYEG proteoliposomes and liposomes. J. Mol. Biol., 406, 362-370. Wang, P. and Dalbey, R. E. (2011). Inserting membrane proteins: the YidC/Oxa1/Alb3 machinery in bacteria, mitochondria, and chloroplasts. Bioc. Biophys. Acta., 1808, 866-875. Dalbey, R. E. and Kuhn, A. (2012). Protein Traffic in Gram-negative bacteria. How exported and secreted proteins find their way. Fems Microbiology Rev., Jan 17, epub of print. Dalbey, R. E., Wang, P. and van Dijl, J. M. (2012). Membrane proteases in the bacterial protein secretion and quality control pathway. Micro. Mol. Biol. Rev. 76, 311-330. Zhu, L., Klenner, C., Kuhn, A. and Dalbey, R. E. (2012) Both YidC and SecYEG are Required for Translocation of the Periplasmic loops 1 and 2 of the Multispanning Membrane Protein TatC. J. Mol. Biol. 424, 354-367. Zhu, L., Wasey, A., White, S. H. and Dalbey, R. E. (2013) Charge-composition Features of a Model Single-span Membrane Proteins that Determine Selection of YidC and SecYEG Translocase Pathways in Escherichia coli. J. Biol. Chem., 288, 7704-7716. Zhu, L., H. Ronald Kaback, and Dalbey, R. E. (2013) YidC- a Molecular Chaperone for LacY Protein Folding via the SecYEG machinery. J. Biol. Chem., 288, in press.