研究领域
在江苏省人兽共患病重点实验室从事研究沙门菌的检测及其沙门菌致病机理研究。参加过国家自然科学基金重点项目《鸡白痢沙门菌致病岛-2效应蛋白致病与免疫的分子机制》，国家自然科学基金《TLR5识别在新城疫黏膜免疫应答中的作用研究》和《TLR5识别以减毒沙门菌为载体的黏膜免疫应答中的作用研究》，公益性行业(农业)科研专项《动物源性沙门菌防控技术与示范》，江苏省自然科学基金《基于TLR5识别途径研究新城疫黏膜疫苗免疫的细胞分子机理》。国家科技支撑计划《动物源性人畜共患细菌病防控生物新制剂的研究》。“863”子课题《畜禽重要细菌病基因工程疫苗的研究和创制》，“973”子课题《重要动物病原菌的分子生物学与致病机理的研究》，国家农业部公益性行业科研专项经费子课题《猪、鸡主要疫病综合免疫技术研究与示范》，教育部FANEDD《以细菌作载体的基因工程黏膜疫苗及其保护性免疫机理研究》专项资金的科学研究。目前，主要从事沙门氏菌减毒疫苗的研制，细菌致病基因的发掘及其功能基因组学，细菌代谢基因组学研究，细菌诱发炎症及致病分子机理等。在此期间，建立了细菌转座突变及细菌基因敲除2个技术平台，为细菌的功能基因的研究奠定良好的基础。利用PCR-STM方法，发掘了10多个沙门菌毒力相关基因，发表论文共50篇。获得转基因微生物安全证书1项，申请专利20项，授权6项。现为江苏省微生物协会理事，江苏省免疫学会会员，《中国家禽》、《中国兽医科学》、《BMCmicrobiology》和《MicrobialPathogenesis》等审稿人。
专利
[1]焦新安,耿士忠等.一种鸡白痢沙门菌单基因无痕敲除减毒株及其制备方法和应用.申请号：201911228849.3
[2]焦新安,耿士忠等.鸡白痢沙门菌三基因敲除减毒突变株制备及其应用.
授权号:ZL201610616590.X
[3]单玉平,耿士忠等.父母代蛋种鸡生产技术规程地方标准DB3207/T114-2018
[4]焦新安,耿士忠等.鸡白痢沙门菌分泌性蛋白的新用途.
授权号:201310104622.3
[5]焦新安,耿士忠等.禽流感病毒多重PCR检测方法.授权号:ZL200410014289
[6]焦新安,耿士忠等.一种用于蓝白斑筛选的自杀载体pGMB152及其应用.
申请号:CN201410054058
[7]焦新安,耿士忠等.肠炎沙门氏菌双基因敲除减毒突变株及其制备与应用.
申请号:CN201310405101
[8]焦新安,耿士忠等.分泌沙门菌SpiC蛋白单克隆抗体的杂交瘤细胞株、其单克隆
抗体及其应用.申请号:CN201510275896.9
近期论文
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[1]YaonanWang,YuanCai,JianZhang,DongLiu,XiaoGong,ZhimingPan,ShizhongGengandXin’anJiao.ControversySurroundingtheFunctionofSpiCProteininSalmonella:AnOverviewFront.Microbiol.201910:1784.
[2]DanXiong,LiSong,ShizhongGeng,YangJiao,etal.Salmonella Coiled-Coil-andTIR-ContainingTcpSEvadestheInnateImmuneSystemandSubduesInflammation.CellReports,2019,28(3)804-818
[3]ZhuoyangLi,RongxianGuo,FanWang,ShizhongGeng,ZhimingPan.Inactivationof Salmonella Enteritidisoneggshellsbylacticacidspray.FoodControl,2019(104),201-207
[4]YaxinGuo,YingXu,XilongKang,ChuangMeng,ShizhongGeng,etal.MolecularcloningandfunctionalanalysisofTRAF6fromYangzhougreatwhitegoose Anseranser.Developmental&ComparativeImmunology,2019,101:103435
[5]FeiXiao;YinKequan;ShizhongGengetal.Analysesofprevalenceandmoleculartypingrevealthespreadofantimicrobial-resistantSalmonellainfectionacrosstwobreederchicken.POULTRYSCIENCE,2018, 97(12):4374-4383
[6]LiuWei;JiangYuanyuan;SunJing;ShizhongGeng,etal.ActivationofTGF-beta-activatedkinase1(TAK1)restrictsSalmonellaTyphimuriumgrowthbyinducingAMPKactivationandautophagy.CELLDEATH&DISEASE, 2018,9
[7]ShizhongGeng,YaonanWang,YingXue,HuqiangWang,XinanJiao.TheSseLproteininhibitstheintracellularNF-κBpathwaytoenhancethevirulenceof Salmonella Pulloruminachickenmodel.MicrobialPathogenesis,2019,129:1-6
[8]RongxianGuo,ZhuoyangLi,XiaohuiZhou,CuiyingHuang,ShizhongGengetal.InductionofarthritisinchickensbyinfectionwithnovelvirulentSalmonella Pullorumstrains.VeterinaryMicrobiology,2019,228:165-172
[9]YaxinGuo,YingXu,XilongKang,DanGu,ShizhongGeng,ZhimingPan.Immunogenicpotentialandprotectiveefficacyofa sptP deletionmutantof Salmonella Enteritidisasalivevaccineforchickensagainstalethalchallenge.InternationalJournalofMedicalMicrobiology,2019,151337
[10]ShizhongGeng,QinTian,ShuminAn,ZhimingPan,XiangChen,XinanJiao.High-Efficiency,Two-StepScarless–MarkerlessGenomeGeneticModificationinSalmonellaenterica.CurrMicrobiol,2016,online(IF=1.423)
[11]ShizhongGeng,ZhichengLiu,ZhijieLin,PaulBarrow,ZhimingPan,QiuchunLi,XinanJiao.Identificationofinvivo-inducedgenesduringinfectionofchickenswithsalmonellaentericaserovarenteritidis[J].ResearchinVeterinaryScience,2015,100:1-7.（IF=1.511）
[12]ShizhongGeng,XinanJiao,PaulBarrow,ZhimingPan,XiangChen.VirulencedeterminantsofSalmonellaGallinarumbiovarPullorumidentifiedbyPCRsignature-taggedmutagenesisandthespiCmutantasacandidateliveattenuatedvaccine.VeterinaryMicrobiology,2014,168,(2–4):388-394.(IF=2.726,1区)
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[14]ShizhongGeng,Xin-AnJiao,Zhi-MingPan,etal.AnimprovedmethodtoknockouttheasdgeneofSalmonellaentericaserovarPullorum[J].JournalofBiomedicineandBiotechnology,2009,doi:10.1155/2009/646380，http://www.hindawi.com/journals/jbb/2009/646380.html(IF=2.706)
[15]Chen,Xiang;Zhang,Weiqiu;Yin,Jiajun;Zhang,Ning;Geng,Shizhong;etal.EscherichiacoliisolatesfromsickchickensinChina:Changesinantimicrobialresistancebetween1993and2013.VeterinaryJournal,2014,202(1):112-115.
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[17]YanqingZhou,ZhimingPan,YuchenLi,XilongKang,XiaoquanWang,ShizhongGeng,ZhongyiLiu,XinanJiao,XiufanLiu.EpidemiologicalanalysisofSalmonellaisolatesrecoveredfromfoodanimalsandhumansineasternChina[J].FoodResearchInternational,2013,54(1):223-229.
[18]FangQiang,PanZhiming,GengShizhong,etal.Molecularcloning,characterizationandexpressionofgooseToll-likereceptor5[J].MolecularImmunology,2012,52(3-4):117-124.
[19]HuMaozhi,PanZhiming,YangYun,MengChuang,GengShizhong,etal.Differentantigenpresentationtendenciesofgranulocyte-macrophagecolony-stimulating
factor-inducedbonemarrow-derivedmacrophagesandperitonealmacrophagesinvitro[J].CellularandDevelopmentalBiology-Animal,48(7),2012,434-440.
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[23]H.ZhangK.WenJ.ShenS.Geng,JiaoXinan.CharacterizationofimmuneresponsesfollowingintranasalimmunizationwiththeMycobacteriumbovisCFP-10proteinexpressedbyattenuatedSalmonellatyphimurium[J].ScandinavianJournalofImmunology,2010,72:277-283.
[24]ZhimingPan,XiaomingZhang,ShizhongGeng,JiaoXinan.Prime-BoostimmunizationusingaDNAvaccinedeliveredbyattenuatedSalmonellaentericaSerovarTyphimuriumandakilledvaccinecompletelyprotectschickensfromH5N1highlypathogenicavianinfuenzavirus[J].Clinicalandvaccineimmunology,2010,23(17):518-523.
[25]LeiZhang,ZhimingPan,ShizhongGeng,JiaoXinan.Sensitive,semi-nestedRT-PCRamplicationoffusiongenesequencesfortheRapiddetectionanddifferentiationofNewcastlediseasevirus[J].ResearchinVeterinaryScience,2010,89:282-289.
[26]PanZM,ZhangXM,GengSZ,etal.PrimingwithaDNAvaccinedeliveredbyattenuatedSalmonellatyphimuriumandBoostingwithakilledvaccineconfersprotectionofchickensagainstinfectionWiththeH9subtypeofavianinfluenzavirus[J].Vaccine,2009,27:1018-1023.
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参编书
2014年，焦新安教授主编《沙门菌病》
2012年，秦爱建教授主编《兽医手册》（正在编辑出版）
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