个人简介

ACS Award for Distinguished Service in the Advancement of Inorganic Chemistry, 2005 Biophysical Society Founders Award, 2005 Co-chair, Gordon Conference on Environmental Bio-inorganic Chemistry, 2004 Wellcome Visiting Professor in the Basic Medical Sciences, Univ. of British Columbia, 2000 Editorial Boards: Inorganic Chemistry (1988-90), Protein Science (1992 - 1995), Issues in Environmental Science and Technology (1994- ), BioSpectroscopy (1995- ), Inorganic Biochemistry (1997- ) Co-chair, NSF Workshop on Environmental Chemistry, 1992 AAAS Fellow, 1991 NIH MERIT Award, 1989 Guggenheim Fellow, 1989 Chair, Fourth International Conference on Time-Resolved Vibrational Spectroscopy, Princeton, 1989 Chair, Bioinorganic Subdivision, American Chemical Society, 1988 Bomem-Michelson Award, 1986 Biophysical Society Council, 1986-1989 International Steering Committee for the International Conference on Raman Spectroscopy (ICORS) 1982 - 1992 Eugene Higgins Chair of Chemistry, Princeton University,1981 Dreyfus Distinguished Lecturer, Furman University, 1981 Chair, Gordon Conference on Metals in Biology, 1978 NIH Metallobiochemistry Study Section, 1977-1979 NATO Senior Fellowship, 1972 NIH Postdoctoral Fellowship, 1962-1963 Fulbright Fellowship, 1960-1961 Sigma Xi Phi Beta Kappa

研究领域

Bioinorganic and Biophysical Chemistry

The Spiro group researches in the areas of bioinorganic chemistry, biophysical chemistry, and environmental chemistry. We study the involvement of inorganic elements such as iron, copper, and manganese in biological and environmental processes. The hemoglobin molecule, an exemplar of allostery, is being studied in real time, as it changes its conformation between high- and low-affinity oxygen-binding states.Biological molecules are compared with synthetic analogues using spectroscopic methods, particularly resonance Raman spectroscopy, which allows analysis of light scattered from a sample that is illuminated in an absorption band. The resonance Raman spectrum contains vibrational modes of the light-absorbing part of the molecule and provides a means of monitoring structural features of specific sites in complex systems. In addition, the use of pulsed lasers allows us to monitor transient species in real time, using photolysis or heat (T-jump) pulses to initiate reactions of interest. We are applying these techniques to protein dynamics, examining how proteins unfold, and how they change their shape in response to ligand binding (allostery).

近期论文

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"Probing Soluble Guanylate Cyclase Activation by CO and YC-1 Using Resonance Raman Spectroscopy"; Ibrahim M, Derbyshire ER, Marletta A and Spiro TG ; Biochemistryc ; 2010 ; ASAP ; xx ; xx - xxx "New Light on NO Bonding in Fe(III) Heme Proteins from Resonance Raman Spectroscopy and DFT Modeling"; Soldatova AV, Ibrahim M, Olson JS,Czernuszewicz RS, Spiro TG ; J Am Chem Soc ; 2010 ; 132 ; 13 ; 4614 - 4625 "The exceptionally stable cobalt(III) - desferrioxamine B complex"; Duckworth OW, Bargar JR, Jarzecki AA, Oyerinde O, Spiro TG, Sposito G ; Marine Chemistry ; 2009 ; 113 ; 1-2 ; 114 - 122 "Linking conformation change to hemoglobin activation via chain-selective time-resolved resonance Raman spectroscopy of protoheme/mesoheme hybrids"; Balakrishnan G, Ibrahim M, Mak PJ, Hata J, Kincaid JR, Spiro TG ; J Biol Inorg Chem. ; 2009 ; 14 ; 5 ; 741 - 750 "Subunit-selective interrogation of CO recombination in carbonmonoxy hemoglobin by isotope-edited time-resolved resonance Raman spectroscopy"; Balakrishnan G, Zhao X, Podstawska E, Proniewicz LM, Kincaid JR, Spiro TG ; Biochemistry ; 2009 ; 48 ; 14 ; 3120 - 3126 "Modulation of the heme electronic structure and cystathionine beta-synthase activity by second coordination sphere ligands: The role of heme ligand switching in redox regulation"; Singh S, Madzelan P, Stasser J, Weeks CL, Becker D, Spiro TG, Penner-Hahn J, Banerjee R ; J Inorg Biochem. ; 2009 ; 103 ; 5 ; 689 - 697 "Protein dynamics from time resolved UV Raman spectroscopy"; Balakrishnan, G ; Weeks, CL ; Ibrahim, M ; Soldatova, AV and Spiro, TG ; Current Opinion in Structural Biology ; 2008 ; 18 ; 5 ; 623 - 629 "Characterization of two different five-coordinate soluble guanylate cyclase ferrous-nitrosyl complexes"; Derbyshire, ER ; Gunn, A ; Ibrahim, M ; Spiro, TG ; Britt, RD and Marletta, MA ; Biochemistry ; 2008 ; 47 ; 12 ; 3892 - 3899 "Solution structure of molybdic acid from Raman spectroscopy and DFT analysis"; Oyerinde, OF ; Weeks, CL ; Anbar, AD and Spiro, TG ; Inorganica Chimica Acta ; 2008 ; 361 ; 4 ; 1000 - 1007 "Experimental investigation of the effects of temperature and ionic strength on Mo isotope fractionation during adsorption to manganese oxides"; Wasylenki, LE ; Rolfe, BA ; Weeks, CL ; Spiro, TG and Anbar, AD ; Geochimica Et Cosmochimica Acta ; 2008 ; 72 ; 24 ; 5997 - 6005 "Investigation of an unnatural amino acid for use as a resonance Raman probe: detection limits and solvent and temperature dependence of the nu C equivalent to N band of 4-cyanophenylalanine"; Weeks, CL ; Polishchuk, A ; Getahun, Z ; DeGrado, WF and Spiro, TG ; Journal of Raman Spectroscopy ; 2008 ; 39 ; 11 ; 1606 - 1611 "DFT analysis of axial and equatorial effects on Heme-CO vibrational modes: Applications to CooA and H-NOX heme sensor proteins"; Xu, CL ; Ibrahim, M and Spiro, TG ; Biochemistry ; 2008 ; 47 ; 8 ; 2379 - 2387 "Ambidentate H-bonding by heme-bound NO: structural and spectral effects of -O versus -N H-bonding"; Xu, CL and Spiro, TG ; Journal of Biological Inorganic Chemistry ; 2008 ; 13 ; 4 ; 613 - 621 "Cu(I) recognition via cation-pi and methionine interactions in CusF"; Xue, Y ; Davis, AV ; Balakrishnan, G ; Stasser, JP ; Staehlin, BM ; Focia, P ; Spiro, TG ; Penner-Hahn, JE and O'Halloran, TV ; Nature Chemical Biology ; 2008 ; 4 ; 2 ; 107 - 109 "Adventures in Bioinorganic chemistry"; Spiro, TG ; Inorganic Chemistry ; 2007 ; 46 ; 26 ; 10968 - 10980 "Density functional theory analysis of molybdenum isotope fractionation"; Weeks, CL; Anbar, AD; Wasylenki, LE; Spiro, TG ; JOURNAL OF PHYSICAL CHEMISTRY A ; 2007 ; 111 ; 49 ; 12434 - 12438 "Mechanism of the CO-sensing heme protein CooA: New insights from the truncated heme domain and UVRR spectroscopy"; Ibrahim, M; Kuchinskas, M; Youn, H; Kerby, RL; Roberts, GP; Poulos, TL; Spiro, TG ; JOURNAL OF INORGANIC BIOCHEMISTRY ; 2007 ; 101 ; 11-12 ; 1776 - 1785 "Enthalpic and entropic stages in alpha-helical peptide unfolding, from laser T-Jump/UV raman spectroscopy"; Balakrishnan, G; Hu, Y; Bender, GM; Getahun, Z; DeGrado, WF; Spiro, TG ; JOURNAL OF THE AMERICAN CHEMICAL SOCIETY ; 2007 ; 129 ; 42 ; 12801 - 12808 "Trans-dioxo manganese(V) Porphyrins"; Jin, N; Ibrahim, M; Spiro, TG; Groves, JT ; JOURNAL OF THE AMERICAN CHEMICAL SOCIETY ; 2007 ; 129 ; 41 ; 12416 - + "A conformational switch to beta-sheet structure in cytochrome c leads to heme exposure. Implications for cardiolipin peroxidation and apoptosis"; Balakrishnan, G; Hu, Y; Oyerinde, OF; Su, J; Groves, JT; Spiro, TG ; JOURNAL OF THE AMERICAN CHEMICAL SOCIETY ; 2007 ; 129 ; 3 ; 504 - 505