王宏达 - 中国科学院长春应用化学研究所 - 电分析化学国家重点实验室

个人简介

12/2007 – 至今: 研究员，中国科学院长春应用化学研究所电分析国家重点实验室 09/2001 – 12/2007: 博士后, 美国亚里桑那州立大学生物设计研究所单分子生物物理研究中心 07/2008 – 10/2008: 访问教授, 美国亚里桑那州立大学生物设计研究所单分子生物物理研究中心 03/2001 – 08/2001: 博士后, 德国马普分子生理研究所 (Max-Planck-Institut fur molekulare Physiologie, Germany) 09/1995 – 12/2000: 博士生, 中国科学院长春应用化学研究所 09/1991 – 07/1995: 本科生, 吉林大学分子生物系生物化学专业

研究领域

细胞膜是细胞的天然屏障,它把细胞与外界环境分离开来。细胞膜有许多重要的生物功能, 如物质隔离、物质交换和细胞通讯等。这些功能的实现主要是靠磷脂双层膜和分布在其上的糖蛋白完成的。目前细胞膜的单分子结构还没有完全清楚，尚处于模型假设阶段。当今经典的生物膜模型仍然是1972年Singer等提出的流动镶嵌模型。此模型提出跨膜蛋白随机的分散在磷脂双层膜中，蛋白质就像漂浮的小筏在磷脂层中随机流动，蛋白两端分别伸出细胞内外膜。然而近期的一些研究表明，流动镶嵌模型对一些生物膜现象并不能很好的解释，如信号转导中的多蛋白协作现象。因此一些改进的细胞膜模型已经被逐渐提出，如脂筏模型。事实上，生物膜结构的研究还远没有完善。这原因有很多，其一是细胞膜的复杂性，它是由多种膜蛋白（多达上百种）、脂类和糖类组成的超分子体系，结构十分复杂。其二是研究细胞膜方法的限制。本小组利用现场高分辨AFM、分子识别成像显微镜、荧光显微镜等为手段在生理条件下对生物膜结构进行了系统的研究，包括细胞膜的静态高分辨结构、细胞膜物质转运的动态过程、细胞膜内吞事件以及病毒侵染细胞的动态过程。

近期论文

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Qiuyan Yan, Yanting Lu, Lulu Zhou, Junling Chen, Haijiao Xu, Mingjun Cai, Yan Shi, Junguang Jiang, Wenyong Xiong*, Jing Gao*, Hongda Wang*, Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging, Proceedings of the National Academy of Sciences, 2018, 115(27): 7033-7038. IF: 9.504 Yangang Pan, Yuebin Zhang, Pianchou Gongpan, Qingrong Zhang, Siteng Huang, Bin Wang, BingqianXu, YupingShan*, Wenyong Xiong*, Guohui Li*, Hongda Wang*, Single glucose molecule transport process revealed by force tracing and molecular dynamics simulations，Nanoscale Horizons, 2018, 3(5): 517-524. IF: 9.391 Jing Gao#, Lingli He#, Yan Shi#, Mingjun Cai, Haijiao Xu, Junguang Jiang, Lei Zhang*, Hongda Wang*, Cell Contact and Pressure Control of Yap Localization and Clustering Revealed by Super-Resolution Imaging, Nanoscale, 2017, 9(43):16993-7003. IF: 7.233 Yangang Pan, Fuxian Zhang, Liuyang Zhang, Shuheng Liu, Mingjun Cai, Yuping Shan*, Xiaoqiao Wang*, Hanzhong Wang*, Hongda Wang*, The Process of Wrapping Virus Revealed by a Force Tracing Technique and Simulations. Advanced Science. 2017, 4(9):7. DIO: 10.1002/advs.201600489. IF: 12.441 Junling Chen, Tianzhou Liu, Jing Gao, Lan Gao, Lulu Zhou, Mingjun Cai, Yan Shi, Wenyong Xiong, Junguang Jiang, Ti Tong*, Hongda Wang*, Variation in carbohydrates between cancer and normal cell membranes revealed by super-resolution fluorescence imaging, Advanced Science, 2016, 3(12):9. DIO: 10.1002/advs.201600270. IF: 12.441 Junling Chen, Jing Gao, Mingjun Cai, Haijiao Xu, Junguang Jiang, Zhiyuan Tian, Hongda Wang*, Mechanistic insights into the distribution of carbohydrate clusters on cell membranes revealed by dSTORM imaging, Nanoscale, 2016, 8(28):13611-13619. IF: 7.233 Yuping Shan, Hongda Wang*, The structure and function of cell membranes examined by atomic force microscopy and single-molecule force spectroscopy, Chemical Society Reviews, 2015, 44(11):3617-3638. IF: 40.182 Bohua Ding, Yongmei Tian, Yangang Pan, Yuping Shan, Mingjun Cai, Haijiao Xu, Yingchun Sun*, Hongda Wang*, Recording the dynamic endocytosis of single gold nanoparticles by AFM-based force tracing, Nanoscale, 2015, 7(17):7545-7549. IF: 7.233 Jing Gao, Ye Wang, Mingjun Cai, Yangang Pan, Haijiao Xu, Junguang Jiang Hongbin Ji*, Hongda, Wang*, Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging, Nanoscale, 2015, 7(6):2511-2519. IF: 7.233 Junling Chen, Jing Gao, Jiazhen Wu, Min Zhang, mingjun Cai, Haijiao Xu, Junguang Jiang, Zhiyuan Tian, Hongda, Wang*, Revealing the carbohydrate pattern on the cell surface by super-resolution imaging, Nanoscale, 2015, 7(8), 3373-3380. IF: 7.233 Yangang Pan, Shaowen Wang, Yuping Shan, Dinglin Zhang, Jing Gao, Min Zhang, Shuheng Liu, Mingjun Cai, Haijiao Xu, Guohui Li*, Qiwei Qin*, Hongda Wang*, Ultrafast tracking of a single live virion during the invagination of a cell membrane, Small, 2015, 11(23):2782-2788. IF: 9.598 Ye Wang#, Jing Gao#, Xingdong Guo#, Ti Tong, Xiaoshan Shi, Lunyi Li, Miao Qi, Yajie Wang, Mingjun Cai, Junguang Jiang, Chenqi Xu*, Hongbin Ji*, Hongda Wang*, Regulation of EGFR nanocluster formation by ionic protein-lipid interaction, Cell Research, 2014, 24(8):959-976. IF: 15.393 Yangang Pan, Feng Wang, Yanhou Liu, Junguang Jiang, Yong-Guang Yang*, Hongda Wang*, Studying the mechanism of CD47-SIRP alpha interactions on red blood cells by single molecule force spectroscopy, Nanoscale, 2014, 6(17):9951-9954. IF: 7.233 Heng Liu, Hui Yang, Xian Hao, Haijiao Xu, Debao Xiao*, Hongda Wang*, Zhiyuan Tian*, Development of polymeric nanoprobes with improved lifetime dynamic range and stability for intracellular oxygen sensing, Small, 2013, 9(15):2639-2648. IF: 9.598 Weidong Zhao, Mingjun Cai, Haijiao Xu, Junguang Jiang, Hongda Wang*, Single-Molecule Force Spectroscopy study of the interactions between lectins and carbohydrates on cancer and normal cells, Nanoscale, 2013, 5(8), 3226-3229. IF: 7.233 Weidong Zhao, Shuheng Liu, Mingjun Cai, Haijiao Xu, Junguang Jiang, Hongda Wang*, Detection of carbohydrates on the surface of cancer and normal cells with topography and recognition imaging, Chemical Communications, 2013, 49(29): 2980-2982. IF: 6.29 Mingjun Cai#, Weidong Zhao#, Xin Shang, Junguang Jiang, Hongbin Ji, Zhiyong Tang*, Hongda Wang*, Direct evidence of lipid rafts by in-situ atomic force microscopy, Small, 2012, 8(8):1243-1250. IF: 9.598 Yuping Shan, Jinfeng Huang, Juanjuan Tan, Gui Gao, Shuheng Liu, Hongda Wang*, Yuxin Chen*, The study of single anticancer peptides interacting with HeLa cell membranes by single molecule force spectroscopy, Nanoscale, 2012, 4(4):1283-1286. IF: 7.233 Xian Hao, Xin Shang, Jiazhen Wu, Yuping Shan, Mingjun Cai, Junguang Jiang, Zhong Huang*, Zhiyong Tang*, Hongda Wang*, Single particle tracking of hepatitis-B-virus-like vesicles entry into cells, Small, 2011, 7(9):1212-1218. IF: 9.598 Yuping Shan, Xian Hao, Xin Shang, Mingjun Cai, Junguang Jiang, Zhiyong Tang*, Hongda Wang*，Recording force events of single quantum-dot endocytosis, Chemical Communications, 2011, 47(12):3377-3379. IF: 6.29