周佳海 - 中国科学院深圳先进技术研究院 -

个人简介

Professional experience 2020-now Professor, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences 2006-2020 Professor, State Key Laboratory of Bio-organic Chemistry and Natural Products Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 2000-2001 Assistant Professor, State Key Laboratory of Bio-organic Chemistry and Natural Products Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 1993-1995 Research Assistant, Nanjing Chemical Plant Education 2001-2006 Postdoctoral Research Fellow, University of Michigan 1995-2000 Ph.D., Bioorganic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 1989-1993 B.S., Analytical Chemistry, Nanjing University

研究领域

In-Silico Enzyme Engineering In silico design (ISD) methods are used to discover new biocatalysts and improve existing enzyme performance. Deep learning methods have been increasingly applied to find patterns in data favorable to predicting substrate specificity, and guiding rational design to improve enzyme stability, solubility, and other characteristics. High Throughput Mutant Screening for Repurposed Enzyme High throughput screening or selection methods plays an extremely significant role in successful evolutionary enzyme engineering, which greatly increases the chance of obtaining desired properties and reduce the time and cost. By taking advantage of automation, high throughput screening methods can streamline traditional screening process. Most importantly, designed methods can make the desired mutants easy to detect.

近期论文

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Jun-Bin He, Lian Wu, Wanqing Wei, Song Meng, Zheng-Tao Liu, Xuan Wu, Hai-Xue Pan, Sheng Yang, Yong Liang#, Jiahai Zhou#, Gong-Li Tang#. (2023). Enzymatic catalysis favours eight-membered over five-membered ring closure in bicyclomycin biosynthesis. Nat Catal. Yang, Qin, Zhenkun Zhang, Lin Wang, Xiwen Xing#, Jiahai Zhou#, and Long Li#. (2023). Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening. Nanomaterials, 13(14): 2064. Dong-Dong Wu, Sheng Jin, Ruo-Xiao Cheng, Wen-Jie Cai#, Wen-Long Xue, Qing-Qing Zhang, Le-Jie Yang, Qi Zhu, Meng-Yao Li, Ge Lin, Yi-Zhen Wang, Xue-Pan Mu, Yu Wang, Igor Ying Zhang, Qi Zhang, Ying Chen, Sheng-Yang Cai, Bo Tan, Ye Li, Yun-Qian Chen, Pu-Juan Zhang, Chen Sun, Yue Yin, Ming-Jie Wang, Yi-Zhun Zhu, Bei-Bei Tao, Jia-Hai Zhou#, Wei-Xue Huang#, and Yi-Chun Zhu#. (2023). Hydrogen sulfide functions as a micro-modulator bound at the copper active site of Cu/Zn-SOD to regulate the catalytic activity of the enzyme. Cell Reports, 42, (7): 112750. Liu J., Lu J., Zhang C., Zhou Q., Jamieson C.S., Shang C., Houk K.N.#, Zhou J.#, Hu Y.#(2023). Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis. Nat. Chem. Xu X.#, Yang Q., Wang L., Zheng J., Gu Y., Xing X.#, Zhou J.# (2023) Enzymatic hydrolysis on L-azetidine-2-carboxylate ring opening. Catal Sci & Technol, 10.1039/D3CY00366C Ohashi, M., D. Tan, J. Lu, C. S. Jamieson, D. Kanayama, J. Zhou#, K. N. Houk# and Y. Tang# (2023) Enzymatic cis-decalin formation in natural product biosynthesis. J Am Chem Soc, 145(6): 3301-3305. Xu, X.#, L. Yang, X. Zhang, X. Xing, J. Zhou# (2023) Characterization and structural basis of D-cysteine desulfhydrase from Pectobacterium atrosepticum. Tetrahedron, 130:133174. Wu P., Y. Gu, L. Liao, Y. Wu, J. Jin, Z. Wang, J. Zhou, S. Shaik#, B. Wang# (2022) Coordination Switch Drives Selective C−S Bond Formation by the Non-heme Sulfoxide Synthases. Angew Chem Int Edit, 61(50): e202214235. Wang L., X. Zang, J. Zhou (2022) Synthetic biology: A powerful booster for future agriculture. Advanced Agarochem,1(1): 7-11. Zhang, J., X. Hou, Z. Chen, Y. Ko, M. W. Ruszczycky, Y. Chen, J. Zhou# and H. Liu# (2022) Dioxane bridge formation during the biosynthesis of spectinomycin involves a twitch radical SAM dehydrogenase that may have evolved from an epimerase. J Am Chem Soc, 144(22): 9910-9919. Huang, X., J. Feng, J. Cui, G. Jiang, W. Harrison, X. Zang, J. Zhou, B. Wang# and H. Zhao# (2022) Photoinduced chemomimetic biocatalysis for enantioselective intermolecular radical conjugate addition. Nat Catal, 5: 586–593. Zhang, X., Z. Xu, J. Zhou, X. Xing# and L. Li# (2022) Enhancement of protein crystallization using nano-sized metal–organic framework. Crystals, 12(5): 578. Lee, Y., X. Hou, R. Chen, J. Feng, X. Liu, M. W. Ruszczycky, J. Gao#, B. Wang#, J. Zhou# and H. Liu# (2022) Radical S-Adenosyl methionine enzyme BlsE catalyzes a radical-mediated 1,2-diol dehydration during the biosynthesis of Blasticidin S. J Am Chem Soc, 144(10): 4478-4486. Jin, J., J. Zhou (2022) The mystery of Z-genome biosynthesis has been elucidated. Syn Bio J, 3(1): 1-5. Chen, T., Z. Zheng, X. Zhang, J. Chen, L. Cha, Y. Tang, Y. Guo#, J. Zhou#, B. Wang#, H. Liu# and W. Chang# (2022) Deciphering the reaction pathway of mononuclear iron enzyme-catalyzed N≡C triple bond formation in isocyanide lipopeptide and polyketide biosynthesis. Acs Catal, 12(4): 2270-2279. Wu, L., Z. Wang, Y. Cen#, B. Wang# and J. Zhou# (2022) Structural insight into the catalytic mechanism of the endoperoxide synthase FtmOx1. Angew Chem Int Ed Engl, 61(12): e202112063. Cheng, Q., Y. Cheung, C. Liu, Q. Xiao, B. Sun, J. Zhou, E. W. C. Chan, R. Zhang and S. Chen (2021) Structural and mechanistic basis of the high catalytic activity of monooxygenase Tet(X4) on tigecycline. BMC Biol, 19(1): 262. Wen, W., Y. Zhang, Y. Zhang, Q. Yu, C. Jiang, M. Tang, J. Pu, L. Wu, Y. Zhao, T. Shi, J. Zhou# and G. Tang# (2021) Reductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics. Nat Commun, 12(1): 7085. Li, S., P. D. Bury, F. Huang, J. Guo, G. Sun, A. Reva, C. Huang, X. Jian, Y. Li, J. Zhou, Z. Deng, F. J. Leeper#, P. F. Leadlay#, M. V. B. Dias# and Y. Sun# (2021) Mechanistic insights into dideoxygenation in gentamicin biosynthesis. Acs Catal, 11(19): 12274-12283. Xie, L., X. Zang, W. Cheng, Z. Zhang, J. Zhou#, M. Chen# and Y. Tang# (2021) Harzianic acid from Trichoderma afroharzianum is a natural product inhibitor of acetohydroxyacid synthase. J Am Chem Soc, 143(25): 9575-9584. Xiang, M. Wang, Wu, Z. Lu, J. Tang, J. Zhou, W. Huang#, and G. Zhang# (2021) Structural insights into xylanase mutant 254RL1 for improved activity and lower pH optimum, Enzyme Microb Technol, 147: 109786. Liu, N., L. Wu, J. Feng, X. Sheng#, J. Li, X. Chen, J. Li, W. Liu, J. Zhou#, Q. Wu#, and D. Zhu# (2021) Crystal structures and catalytic mechanism of L-erythro-3,5-diaminohexanoate dehydrogenase and rational engineering for asymmetric synthesis of β-amino acids, Angew Chem Int Ed, 60: 10203-10. Gao, D., C. S. Jamieson, G. Wang, Y. Yan, J. Zhou#, K. N. Houk#, and Y. Tang# (2021) A polyketide cyclase that forms medium-ring lactones. J Am Chem Soc, 143: 80-84. Chen, T., J. Chen, Y. Tang, J. Zhou#, Y. Guo# and W. Chang# (2021) Current understanding toward isonitrile group biosynthesis and mechanism. Chin J Chem, 39(2): 463-472. Yan, Y., X. Zang, C. S. Jamieson, H. Lin, K. N. Houk, J. Zhou# and Y. Tang# (2020) Biosynthesis of the fungal glyceraldehyde-3-phosphate dehydrogenase inhibitor heptelidic acid and mechanism of self-resistance. Chem Sci, 11(35): 9554-9562. Ohashi, M., C. S. Jamieson, Y. Cai, D. Tan, D. Kanayama, M. C. Tang, S. M. Anthony, J. V. Chari, J. S. Barber, E. Picazo, T. B. Kakule, S. Cao, N. K. Garg, J. Zhou#, K. N. Houk# and Y. Tang# (2020) An enzymatic Alder-ene reaction. Nature, 586: 64-69. Chen, C., L. Su#, L. Wu, J. Zhou and J. Wu# (2020) Enhanced the catalytic efficiency and thermostability of maltooligosyltrehalose synthase from Arthrobacter ramosus by directed evolution. Biochem Eng J, 162: 107724. Cheng, R., L. Wu, R. Lai, C. Peng, N. Naowarojna, W. Hu, X. Li, S. A. Whelan, N. Lee, J. Lopez, C. Zhao, Y. Yong, J. Xue, X. Jiang, M. W. Grinstaff, Z. Deng, J. Chen, Q. Cui#, J. Zhou# and P. Liu# (2020) Single-step replacement of an unreactive C-H bond by a C-S bond using polysulfide as the direct sulfur source in anaerobic ergothioneine biosynthesis. ACS Catal, 10(16): 8981-8994. Dong, Q., S. Yuan, L. Wu, L. Su, Q. Zhao, J. Wu, W. Huang# and J. Zhou# (2020) Structure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate. Bioresour and Bioprocess, 7(1): 49. Yang, X., L. Wu, A. Li, L. Ye#, J. Zhou# and H. Yu# (2020) The engineering of decameric D-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions. Chem Commun (Camb), 56(55): 7561-7564. Li, A., C. G. Acevedo-Rocha, L. D'Amore, J. Chen, Y. Peng, M. Garcia-Borras, C. Gao, J. Zhu, H. Rickerby,S. Osuna#, J. Zhou# and M. T. Reetz# (2020) Regio- and stereoselective steroid hydroxylation at C7 by cytochrome P450 monooxygenase mutants. Angew Chem Int Ed Engl, 59(30): 12499-12505. Chen, T., J. Chen, Y. Tang, J. Zhou#, Y. Guo# and W. Chang# (2020) Pathway from N-alkylglycine to alkylisonitrile catalyzed by Iron (II) and 2-oxoglutarate-dependent oxygenases. Angew Chem Int Ed Engl, 59(19): 7367-7371. Cai, Y., Y. Hai, M. Ohashi, C. S. Jamieson, M. Garcia-Borras, K. N. Houk#, J. Zhou# and Y. Tang# (2019) Structural basis for stereoselective dehydration and hydrogen-bonding catalysis by the SAM-dependent pericyclase LepI. Nat Chem, 11(9): 812-820. Cen, Y., W. Singh, M. Arkin, T. S. Moody, M. Huang, J. Zhou#, Q. Wu# and M. T. Reetz# (2019) Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution. Nat Commun, 10(1): 3198. Xu, J., Y. Cen, W. Singh, J. Fan, L. Wu, X. Lin, J. Zhou#, M. Huang#, M. T. Reetz# and Q. Wu# (2019) Stereodivergent protein engineering of a lipase to access all possible stereoisomers of chiral esters with two stereocenters. J Am Chem Soc, 141(19): 7934-7945. Zhou, J., Y. Wang, G. Xu, L. Wu, R. Han, U. Schwaneberg, Y. Rao, Y. L. Zhao, J. Zhou# and Y. Ni# (2018) Structural insight into enantioselective inversion of an alcohol dehydrogenase reveals a "polar gate" in stereorecognition of diaryl ketones. J Am Chem Soc, 140(39): 12645-12654. Yan, Y., Q. Liu, X. Zang, S. Yuan, U. Bat-Erdene, C. Nguyen, J. Gan, J. Zhou#, S. E. Jacobsen# and Y. Tang# (2018) Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action. Nature, 559(7714): 415-418. Li, Y., W. Zhang, H. Zhang, W. Tian, L. Wu, S. Wang, M. Zheng, J. Zhang, C. Sun, Z. Deng, Y. Sun, X. Qu# and J. Zhou# (2018) Structural basis of a broadly selective acyltransferase from the polyketide synthase of splenocin. Angew Chem Int Ed Engl, 57(20): 5823-5827. Sun, Z., L. Wu, M. Bocola, H. C. S. Chan, R. Lonsdale, X. D. Kong, S. Yuan#, J. Zhou# and M. T. Reetz# (2018) Structural and computational insight into the catalytic mechanism of limonene epoxide hydrolase mutants in stereoselective transformations. J Am Chem Soc, 140(1): 310-318.