研究领域
1.天然药物活性成分的分离分析及药效学研究：针对中药复杂体系中活性成分的快速识别和分离制备，研究高效液相色谱、高速逆流色谱、超临界流体萃取等在复杂体系物质分离中的理论，建立了无机配合物和天然有机物分离的制备色谱数学模型；创立了多种联用色谱新技术用于快速识别复杂体系中的活性化合物；开展细胞及基因水平上的药效学研究；为新型活性物质的快速识别、提取制备、药物构效关系研究及中药组方规律的揭示提供理论和实验依据。在此方向主持国家自然科学基金3项、省部级科研计划项目4项，发表SCI论文70余篇。
2.手性药物对映体的分离分析：研究色谱分离、膜分离、萃取分离、泡沫分馏分离等各种手性药物对映体分离分析新技术新方法及相关理论。在此方向主持国家自然科学基金2项、湖南省科技平台建设专项1项，发表SCI论文50余篇。
3.药物与蛋白作用机理研究：在分子水平上阐明天然活性小分子与蛋白等生物大分子的非共价弱相互作用, 建立了基于荧光猝灭的二项式分布模型用于研究药物小分子和蛋白弱相互作用。研究受体和供体间的相互识别和作用机制、小分子在体内的代谢过程及其代谢动力学，明确这些成分对代谢性疾病产生、发展和治疗的影响，为药学、临床医学研究提供重要的信息。
4.过程分析仪器：根据具体的分析对象和用户要求，研制基于流动分析的分析体系，采用适当的检测器和检测手段，如分光光度、原子吸收、色谱、离子选择电极、光度滴定、电位滴定等；联用流动注射（FI）的方法实现溶液湿化学处理过程的自动化；设计配套相应的硬件和软件，微机控制整机操作。在2~5分钟内可完成一个样品的分析，从采样、测定到结果计算全部实现自动化。在此方向主持企业合作科研项目2项，发表SCI论文近10篇。
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Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold. Anal Chem, 86 (2014), 7337-7342.
Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification. Anal Chem, 86 (2014), 7843-7848
Improved enantioseparation via the twin-column based recycling high performance liquid chromatography. J Chromatogr A (2014), DOI: 10.1016/j.chroma.2014.07.040.
Systematic and efficient separation of 11 compounds from Rhizoma Chuanxiong via counter-current chromatography–solid phase extraction–counter-current chromatography hyphenation. J Chromatogr A (2014) DOI: 10.1016/j.chroma.2014.08.087.
Separation and purification of bovine serum albumin binders from Fructus polygoni orientalis using off-line two-dimensional complexation high-speed counter-current chromatography target-guided by ligand fishing, J Chromatogr A 1304 (2013) 183-193.
Structures Required of Polyphenols for Inhibiting Advanced Glycation end Products Formation, Curr Drug Metab 14 (2013) 414-431.
Synthesis of Pyrrolo (spiro-[2.3′]-oxindole)-spiro-[4.3 ″]-oxindole via 1, 3-Dipolar Cycloaddition of Azomethine Ylides with 3-Acetonylideneoxindole. J Org Chem, 78(2013), 11577-11583.
Analysis of tyrosinase binders from Glycyrrhiza uralensis root: Evaluation and comparison of tyrosinase immobilized magnetic fishing-HPLC and reverse ultrafiltration-HPLC, J Chromatogr B 932 (2013) 19-25.
On-line two-dimensional LC: A rapid and efficient method for the determination of enantiomeric excess in reaction mixtures, J Sep Sci 36(2013), 3158-3164.
Advance in Dietary Polyphenols as -Glucosidases Inhibitors: A Review on Structure-Activity Relationship Aspect, Crit Rev Food Sci 53 (2013) 818-836.
Preparation of diamine modified mesoporous silica on multi-walled carbon nanotubes for the adsorption of heavy metals in aqueous solution, Appl Surf Sci 282 (2013) 38-45.
Molecularly imprinted polymers coated on multi-walled carbon nanotubes through a simple indirect method for the determination of 2,4-dichlorophenoxyacetic acid in environmental water, Appl Surf Sci 284 (2013) 692-699.
Enantioselective separation of tryptophan by Mg–Al layered double hydroxides intercalated with tartaric acid derivative, Appl Clay Sci 75–76 (2013) 92-99.
Influence of glucose on the human serum albumin-flavone interaction and their antioxidant activity, Mol Biosyst 9 (2013) 55-60.
Extraction of Tryptophan enantiomers by aqueous two-phase systems of ethanol and (NH4)2SO4, Journal of Chemical Technology & Biotechnology 88 (2013) 1545-1550.
Comparative evaluation of three methods based on high-performance liquid chromatography analysis combined with a 2,2′-diphenyl-1-picrylhydrazyl assay for the rapid screening of antioxidants from Pueraria lobata flowers, Anal Bioanal Chem 402 (2012) 2965-2976.
Glycation of plasma proteins in type II diabetes lowers the non-covalent interaction affinities for dietary polyphenols, Integr Biol-Uk 4 (2012) 502-507.
Effect of CdTe QDs on the protein-drug interactions, Nanotoxicology 6 (2012) 304-314.
The influence of flavonoids on the binding of pantoprazole to bovine serum albumin by spectroscopic methods: With the viewpoint of food/drug interference, Food Chem 135 (2012) 1083-1090.
Influence of dietary flavonoids on the glycation of plasma proteins, Mol Biosyst 8 (2012) 2183-2187.
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