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个人简介

1984北京师范大学化学系,理学学士 1997中国科学院化学研究所,理学硕士 2005中国科学院研究生院,理学博士 1984-1986北京师范大学化学系,助教 1986-今中国科学院化学研究所活体分析化学实验室,助理研究员(1989)、副研究员(1996)、项目研究员(2006)、研究员、博士生导师(2008) 1993-1995日本多肽研究所,从事多肽、糖肽的合成与分析研究 1999.12-2000.1日本大阪大学蛋白质研究所,从事多肽的质谱分析研究 中国色谱学会理事,北京色谱学会秘书长,中国仪器仪表学会分析仪器学会理事。

研究领域

研究方向: 1.肽识别分子的设计、合成、筛选与生物分析应用 2.生物分子动态相互作用研究 3.复杂体系高选择性分离分析 1.多肽分子探针的设计、筛选与应用(组合化学肽库的设计与合成,基于分子识别的高效亲和筛选,多肽探针的修饰,多肽探针的活细胞成像检测) 2.石英晶体微天平(QCM)生物传感器(生物分子动态相互作用研究,生物分子高效、高灵敏、免标记检测) 3.生化分离分析新介质的研究(均匀球形高性能分离介质,分子印迹聚合物(MIP),有机/机杂化核壳型磁性微纳颗粒,高效、高选择性亲和分离介质) 4.多肽的微流控芯片阵列合成与筛选检测研究(基于连续流动微流控芯片的多肽阵列固相合成新方法,多单元集成化微流控多肽筛选检测芯片系统)

近期论文

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论文著作 1.YuanyuanZhu,YanyanHuang*,YulongJin,ShilangGui,RuiZhao*, Peptide-guidedsystemwithprogrammablesubcellulartranslocationfortargetedtherapyandbypassingmultidrugresistance, Anal.Chem.,2019,91,1880-1886. 2.ShilangGui,YanyanHuang*,YuanyuanZhu,YulongJin,RuiZhao, BiomimeticsensingsystemfortracingPb2+distributioninlivingcellsbasedonthemetal-peptidesupramolecularassembly, ACSAppl.Mater.Interfaces,2019,11,5804-5811. 3.ShilangGui,YanyanHuang*,FangHu,YulongJin,GuanxinZhang,DeqingZhang,RuiZhao*, BioinspiredpeptideforimagingHg2+distributioninlivingcellsandzebrafishbasedoncoordination-mediatedsupramolecularassembling, Anal.Chem.,2018,90,9708-9715. 4.YuFang,XizhenLian,YanyanHuang,GuoFu,ZhifengXiao,QiWang,BeiyanNan,Jean-PhilippePellois*,Hong-CaiZhou*, Investigatingsubcellularcompartmenttargetingeffectofporouscoordinationcagesforenhancingcancernanotherapy, Small,2018,14,1802709。 5.XizhenLian,YanyanHuang,YuanyuanZhu,YuFang,RuiZhao,ElizabethJoseph,JialuoLi,Jean-PhilippePellois,Hong-CaiZhou*, Enzyme‐MOFnanoreactoractivatesnontoxicparacetamolforcancertherapy, Angew.Chem.Int.Ed.,2018,57,5725-5730. 6.JiayuanHe,ShilangGui,YanyanHuang*,FangHu,YulongJin,YangYu,GuanxinZhang,DeqingZhang*,RuiZhao*, Rapid,sensitive,andin-solutionscreeningofpeptideprobesfortargetedimagingoflivecancercellsbasedonpeptiderecognition-inducedemission, Chem.Commun.,2017,53,11091-11094. 7.YulongJin,YunfengXie,KuiWu,YanyanHuang*,FuyiWang*,RuiZhao*, ProbingthedynamicinteractionbetweendamagedDNAandacellularresponsiveproteinusingapiezoelectricmassbiosensor, ACSAppl.Mater.Interfaces,2017,9,8490-8497. 8.YangYu,YanyanHuang*,YulongJin,RuiZhao*, Dual-targetingpeptideprobeforsequence-andstructure-sensitivesensingofserumalbumin, Biosens.Bioelectron,2017,94,657-662. 9.LeSheng,YulongJin*,YonghuanHe,YanyanHuang,LiushuiYan*,RuiZhao*, Well-definedmagneticsurfaceimprintednanoparticlesforselectiveenrichmentof2,4-dichlorophenoxyaceticacidinrealsamples, Talanta,2017,174,725-732. 10.LimingSu,YulongJin*,YanyanHuang,RuiZhao*, Surface-imprintedmagneticnanoparticlesfortheselectiveenrichmentandfastseparationoffluoroquinolonesinhumanserum, J.Sep.Sci.,2017,40,2269–2277. 11.瑙甘,金钰龙*,黄嫣嫣,赵睿*. 脑啡肽的固相合成与LC-MS/MS分离鉴定, 分析测试学报,2017,36,190-195. 12.YanyanHuang,GuanxinZhang*,FangHu,YulongJin,RuiZhao*,DeqingZhang*, Emissivenanoparticlesfrompyridinium-substitutedtetraphenylethylenesalts:imagingandselectivecytotoxicitytowardscancercellsinvitroandinvivobyvaryingcounteranions, Chem.Sci.,2016,7,7013-7019. 13.YangYu,YanyanHuang*,FangHu,YulongJin,GuanxinZhang,DeqingZhang*,RuiZhao*, Self-assemblednanostructuresbasedonactivatableredfluorescentdyeforsite-specificproteinprobingandconformationaltransitiondetection, Anal.Chem.,2016,88,6374-6381. 14.YanyanHuang,YulongJin,RuiZhao*, Rationaldesignandfunctionalevolutionoftargetedpeptidesforbioanalyticalapplications, Sci.China-Chem.,2016,59,1250-1257. 15.HuiqingHou,LimingSu,YanyanHuang,YulongJin,RuiZhao*, Recentapplicationsofsurfacemolecularlyimprintingmaterialsandtechniquesforseperationandanalysis, ChineseJournalofChromatography,2016,34,1206-1214.(invited) 16.NingZhang,JianHou(a,co-firstauthor),SumingChen,CaiqiaoXiong,HuihuiLiu,YulongJin,JianingWang,QingHe,RuiZhao,ZongxiuNie*, Rapidlyprobingantibacterialactivityofgrapheneoxidebymassspectrometry-basedmetabolitefingerprinting, Sci.Rep.,2016,6,28045. 17.YangYang,YanyanHuang,GuanxinZhang*,RuiZhao,DeqingZhang*, Anewfluorometricturn-onassayforcarboxylesteraseandinhibitorscreeningbasedonaggregationinducedemissionbehavioroftetraphenylethylenemolecules, Acta.Chimica.Sinica.,2016,74,871-876. 18.YulongJin,YanyanHuang*,HuaYang,GuoquanLiu,RuiZhao*, Apeptide-basedpH-sensitivedrugdeliverysystemfortargetedablationofcancercells, Chem.Commun.,2015,51,14454-14457. 19.YanyanHuang,QundanZhang,GuoquanLiu,RuiZhao*, Acontinuous-flowmassbiosensorforthereal-timedynamicanalysisofproteaseinhibition, Chem.Commun.,2015,51,6601-6604. 20.ShilangGui,YanyanHuang,FangHu,YulongJin,GuanxinZhang,LiushuiYan*,DeqingZhang*,RuiZhao*, Fluorescenceturn-onchemosensorforhighlyselectiveandsensitivedetectionandbioimagingofAl3+inlivingcellsbasedonion-inducedaggregation, Anal.Chem.,2015,87,1470-1474. 21.黄嫣嫣、赵睿*, 靶向多肽识别与高选择性分离分析, 色谱,2015,33,447-448(invited聚焦). 22.YanyanHuang,FangHu(a,co-firstauthor),RuiZhao*,GuanxinZhang*,HuaYang,DeqingZhang*, Tetraphenylethyleneconjugatedwithaspecificpeptideasafluorescenceturn-onbioprobeforthehighlyspecificdetectionandtracingoftumormarkersinlivecancercells, Chem.Eur.J.,2014,20,158-164. 23.FangHu,YanyanHuang(a,co-firstauthor),GuanxinZhang,RuiZhao*,HuaYang*,DeqingZhang*, Targetedbioimagingandphotodynamictherapyofcancercellswithanactivatableredfluorescentbioprobe, Anal.Chem.,2014,86,7987-7995. 24.YonghuanHe,YanyanHuang*,YulongJin,XiangjunLiu,GuoquanLiu,RuiZhao*, Well-definednanostructuredsurface-imprintedpolymersforhighlyselectivemagneticseparationoffluoroquinolonesinhumanurine, ACSAppl.Mater.Interfaces,2014,6,9634-9642. 25.YongliangLiu,YonghuanHe,YulongJin,YanyanHuang,GuanquanLiu,RuiZhao*, Preparationofmonodispersedmacroporouscore-shellmolecularlyimprintedparticlesandtheirapplicationinthedeterminationof2,4-dichlorophenoxyaceticacid, J.Chromatogr.A,2014,1323,11-17. 26.YunfengXie,YulongJin,YanyanHuang,GuoquanLiu,RuiZhao*, Highlyselectivepiezoelectricsensorforlead(II)basedonthelead-catalyzedreleaseofgoldnanoparticlesfromaself-assemblednanosurface, Microchim.Acta,2014,181,1521-1527. 27.FangHu,YanyanHuang,GuanxinZhang*,RuiZhao,DeqingZhang*, Ahighlyselectivefluorescenceturn-ondetectionofhydrogenperoxideandD-glucosebasedontheaggregation/deaggregationofamodifiedtetraphenylethylene, TetrahedronLett.,2014,55,1471-1474. 28.YulongJin,YanyanHuang*,GuoquanLiu,RuiZhao*, Goldnanoparticle-sensitizedquartzcrystalmicrobalancesensorforrapidandhighlyselectivedeterminationofCu(II)ion, Analyst,2013,138,5479-5485. 29.WeizhiWang,YanyanHuang,YulongJin,GuoquanLiu,YiChen,HuiminMa,RuiZhao*, Atetra-layermicrofluidicsystemforpeptideaffinityscreeningthroughintegratedsampleinjection, Analyst,2013,138,2890-2896. 30.JizhongLiu,YanyanHuang,BoYang,JianhuaChang,GuoquanLiu,RuiZhao*, PreparationofhighlyhydrophilicstrongcationexchangersbasedonPGMAmatrixandtheirapplicationsinproteinanalysis, ChineseJournalofChromatography,2013,31,310-316. 31.YongliangLiu,YanyanHuang,JizhongLiu,WeizhiWang,GuoquanLiu,RuiZhao*, Superparamagneticsurfacemolecularlyimprintednanoparticlesforwater-solublepefloxacinmesylatepreparedviasurfaceinitiatedatomtransferradicalpolymerizationanditsapplicationineggsampleanalysis, J.Chromatogr.A,2012,1246,15-21. 32.YulongJin,YanyanHuang*,YunfengXie,WenbinHu,FuyiWang,GuoquanLiu,RuiZhao*, Cyclicinterconversionofmethioninecontainingpeptidebetweenoxidizedandreducedphasesmonitoredbyreversed-phaseHPLCandESI-MS/MS, Talanta,2012,89,531-536. 33.ZhibingShi,DanZhao,YanyanHuang,YunDu,XiangrongCao,ZhunanGong,RuiZhao,JianxinLi*, Discovery,synthesis,andevaluationofsmall-moleculesignaltransducerandactivatoroftranscription3inhibitors, Chem.Pharm.Bull.,2012,60,1574-1580. 34.YanyanHuang,RuiZhao*, Highlyselectiveanalysisofproteinsbasedontargetablepeptideprobes, JournalofInstrumentalAnalysis,2012,31,1184-1190(invitedreview). 35.WeizhiWang,YanyanHuang,JizhongLiu,YufengXie,RuiZhao*,ShaoxiangXiong,GuoquanLiu,YiChen,HuiminMa. IntegratedSPPSoncontinuous-flowradialmicrofluidicchip, LabChip,2011,11,929-935. 36.YanyanHuang,ShaoxiangXiong,GuoquanLiu,RuiZhao*, ArapidandhighlyselectivecolorimetricmethodfordirectdetectionoftryptophaninproteinsviaDMSOacceleration, Chem.Commun.,2011,47,8319-8321. 37.YanyanHuang,RuiZhao*,YabinFu,QundanZhang,ShaoxiangXiong,LiLi,RouliZhou*,GuoquanLiu,YiChen, Highlyspecifictargetingandimagingoflivecancercellsusingapeptideprobedevelopedfromrationallydesignedpeptides, ChemBioChem,2011,12,1209-1215. 38.JizhongLiu,WeizhiWang,YunfengXie,YanyanHuang,YongliangLiu,XiangjunLiu,RuiZhao*,GuoiquanLiu,YiChen. Anovelpolychloromethylstyrenecoatedsuperparamagneticsurfacemolecularlyimprintedcore-shellnanoparticleforbisphenolA, J.Mater.Chem.,2011,21,9232-9238. 39.YunfengXie,YanyanHuang,WeizhiWang,GuoquanLiu,RuiZhao*, Dynamicinteractionbetweenmelamineandcyanuricacidinartificialurineinvestigatedbyquartzcrystalmicrobalance, Analyst,2011,136,2482-2488. 40.ChengWang,QunChen,FeiSun,DeqingZhang*,YanyanHuang,RuiZhao,DaobenZhu, Multistimuliresponsiveorganogelsbasedonanewgelatorfeaturingtetrathiafulvaleneandazobenzenegroups,reversibletuningofthegel-soltransitionbyredoxreactionsandlightirradiation, J.Am.Chem.Soc.,2010,132,3092-3096. 41.XiangjunLiu,JizhongLiu,YanyanHuang,RuiZhao*,GuoquanLiu,YiChen, Determinationofmethotrexateinhumanserumbyhigh-performanceliquidchromatographywithpseudotemplatemolecularlyimprintedpolymer, J.Chromatogr.A,2009,1216,7533-7538. 42.LihuaPeng,GuanxinZhang,DeqingZhang*,JunfengXiang,RuiZhao,YilinWang,DaobenZhu, Afluorescence“turn-on”ensembleforacetylcholine-steraseactivityassayandinhibitorscreening, Org.Lett.,2009,4014-4017. 43.XioahongCheng,XiangjunLiu,TaoBing,RuiZhao,ShaoxiangXiong,DihuaShangguan*, SpecificDNAG-Quadruplexesbindtoethanolamines, Biopolymers,2009,91,874-883. 44.QundanZhang,YanyanHuang,RuiZhao*,GuoquanLiu,YiChen, DeterminingbindingsitesofdrugsonhumanserumalbuminusingFIA-QCM, Biosens.Bioelectron.,2008,24,48-54. 45.YanyanHuang,RuiZhao*,JiaLuo,ShaoxiangXiong,DihuaShangguan,HongwuZhang,GuioquanLiu,YiChen, Design,synthesisandscreeningofantisensepeptidebasedcombinatorialpeptidelibrariestowardsanaromaticregionofSARS-CoV, J.Mol.Recognit.,2008,21,122-131. 46.QundanZhang,YanyanHuang,RuiZhao*,GuoquanLiu,YiChen, In-situgrowthofnanogoldonquartzcrystalmicrobalanceanditsapplicationintheinteractionbetweenheparinandantithrombinIII, J.ColloidInterfaceSci.,2008,319,94-99. 47.JiaLuo,QundanZhang,YanyanHuang,GuoquanLiu,RuiZhao*, Quartzcrystalmicrobalancebiosensorforrecombinanthumaninterferon-βdetectionbasedonantisensepeptideapproach, Anal.Chim.Acta,2007,590,91-97. 48.XiangjunLiu,ZhiyongChen,RuiZhao*,DihuaShangguan,GuoquanLiu,YiChen, Uniform-sizedmolecularlyimprintedpolymerformetsulfuron-methylbyone-stepswellingandpolymerizationmethod, Talanta,2007,71,1205-1210. 49.GuoyongWen,DeqingZhang*,YanyanHuang,RuiZhao,LingyunZhu,ZhigangShuai,DaobenZhu*, Newheterocyclictetrathiafulvalenecompoundswithanazobenzenemoiety,photomodulationoftheelectron-donatingabilityofthetetrathiafulvalenemoiety, J.Org.Chem.,2007,72,6247-6250. 50.JiaLuo,YanyanHuang,ShaoxiangXiong,GuoquanLiu,RuiZhao*, Studyonpeptide-peptideinteractionusinghigh-performanceaffinitychromatographyandquartzcrystalmicrobalancebiosensor, Chin.Sci.Bull.,2007,52,1311-1319. 51.RuiZhao,GuoquanLiu, Ligandscreeninginaffinitychromatographyanditsapplications, ChineseJournalofChromatography,2007,25,135-141(invitedreview). 52.XiangjunLiu,CanbinOuyang,RuiZhao*,DihuaShangguan,YiChen,GuoquanLiu, Monolithicmolecularlyimprintedpolymerforsulfamethoxazoleandmolecularrecognitionpropertiesinaqueousmobilephase, Anal.Chim.Acta,2006,571,235-241. 53.RuiZhao*,CanliangFang,XiaoYu,YangLiu,JiaLuo,DihuaShangguan,ShaoxiangXiong,GuoquanLiu*, ScreeningofinhibitorsforinfluenzaAvirususinghighperformanceaffinitychromatographyandcombinatorialpeptidelibraries, J.Chromatogr.A,2005,1064,59-66. 54.HongwuZhang,RuiZhao*,ZhiyongChen,DihuaShangguan,GuoquanLiu, QCM–FIAwithPGMAcoatingfordynamicinteractionstudyofheparinandantithrombinIII, Biosens.Bioelectron.,2005,21,121-127. 55.RuiZhao*,JiaLuo,DihuaShangguan,GuoquanLiu, AnovelmatrixforhighperformanceaffinitychromatographyanditsapplicationinthepurificationofantithrombinIII, J.Chromatogr.B,2005,816,175-181. 56.ZhiyongChen,RuiZhao*,DihuaShangguan,GuoquanLiu, Preparationandevaluationofuniform-sizedmolecularlyimprintedpolymerbeadsusedfortheseparationofsulfamethazine, Biomed.Chromatogr.,2005,19,533-538. 57.KuanpingGong,XiaozhangZhu,RuiZhao,ShaoxiangXiong,Lanquan.Mao,*ChuanfengChen*, Rationalattachmentofsynthetictriptyceneorthoquinoneontocarbonnanotubesforelectrocatalysisandsensitivedetectionofthiols, Anal.Chem.,2005,77,8158-8165. 58.YangLiu,WeiZhang,XiaoYu,HongwuZhang,RuiZhao,DihuaShangguan,YanLi,BeifenShen,GuoquanLiu*, Quartzcrystalbiosensorforreal-timekineticanalysisofinteractionbetweenhumanTNF-aandmonoclonalantibodies, Sens.ActuatorB-Chem.,2004,99,416-424. 59.YuDong,JianjunMao,DihuaShangguan,RuiZhao*,GuioquanLiu, Acetylcholinereleaseinthehippocampusduringtheoperantconditionedreflexandthefootshockstimulusinrats, Neurosci.Lett.,2004,369,121-125. 60.YangLiu,XiaoYu,RuiZhao,DihuaShangguan,ZhiyongBo,GuoquanLiu*, RealtimekineticanalysisoftheinteractionbetweenimmunoglobulinGandhistidineusingquartzcrystalmicrobalancebiosensorinsolution, Biosens.Bioelectron.,2003,18,1419-1427. 61.YangLiu,XiaoYu,RuiZhao,DihuaShangguan,ZuyiBo,GuoquanLiu*, Quartzcrystalbiosensorforrealtimemonitoringofmolecularrecognitionbetweenproteinandsmallmolecularmedicinalagents, Biosens.Bioelectron.,2003,19,9-19. 62.YingxinZhao,RuiZhao,DihuaShangguan,GuoquanLiu*, Anewtypeofcapillarycolumnforopentubularelectrochromatography, Electrophoresis,2002,23,2990-2995. 63.DihuaShangguan,YingxinZhao,HuiwanHan,RuiZhao,GuoquanLiu*, Derivatizationandfluorescencedetectionofaminoacidsandpeptideswith9-fluorenylmethylchloroformateonthesurfaceofasolidadsorbent, Anal.Chem.,2001,73,2054-2057. 64.RuiZhao,Xiao.Yu,HongLiu,LailiangZhai,ShaoxiangXiong,TianshengSu,GuoquanLiu, Studyonthedegeneracyofantisensepeptidesusingaffinitychromatography, J.Chromatogr.A,2001,913,421-428. 65.YingxinZhao,RuiZhao*,DihuaShangguan,ShaoxiangXiong,GuoquanLiu, ThreenovelhighperformanceaffinitychromatographicmediafortheseparationofantithrombinIIIfromhumanplasma, Biomed.Chromatogr.,2001,15,487-492.

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