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个人简介

颜玉蓉,女,博士,教授,硕士研究生导师。先后毕业于四川大学和重庆医科大学,获工学士学位和医学科学博士学位。1991年至今,于重庆医科大学检验医学院从事生物分析化学教学和科研工作。长期承担本科生的《卫生化学》、《化学分析与仪器分析》、《生物分析化学》课程的理论和实验教学,研究生的《蛋白质组学》课程的理论教学。 主持国家自然科学基金面上项目1项、重庆市科委项目3项、重庆市教委研究项目6项。共申请发明专利13项,其中授权6项。发表SCI论文30篇,EI论文4篇。共培养硕士研究生16名,其中毕业9名,在读7名。

研究领域

主要研究方向为临床检验新技术的开发及应用,包括分子诊断技术、纳米生物技术和生物传感、活细胞成像技术等,主要涉及新型纳米材料的合成和应用、DNA纳米自组装、荧光和比色分析、电化学和电化学发光、表面等离子共振成像等。

发明专利: (1)基于滚环扩增和金纳米对沙门氏菌invA基因检测的方法,ZL201310665170.7(已授权); (2)基于滚环扩增和超分子适体对可卡因电化学检测的方法,ZL201310665141.0(已授权); (3)一种microRNA比色传感器的制备与应用,ZL201410629839.1(已授权); (4)一种表面等离子体共振成像传感检测系统,ZL201410531657.0(已授权); (5)一种电化学传感器及其制备与应用,ZL201410626360.2(已授权); (6)一种流通池,ZL201410537618.1(已授权)

近期论文

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2019年 1.Que,H.#,Zhang,D.#,Guo,B.,Wang,T.,Wu,H.,Han,D.,Yan,Y.*,2019.Label-freeandultrasensitiveelectrochemicalbiosensorforthedetectionofEBV-relatedDNAbasedonAgDNCs@DNA/AgNCsnanocompositesandlambdaexonuclease-assistedtargetrecycling.BiosensBioelectron143,111610.(IF:9.518) 2.Wang,T.#,Que,H.#,Cheng,W.,Yan,X.,Ma,H.,Liu,P.,Gan,X.,Yan,Y.*,2019.HomogeneousfluorescentbiosensingmethodforDNAmethyltransferaseactivityanalysisandinhibitorscreeningbasedonhighlyefficientisothermalamplification.SensorsandActuatorsB:Chemical296.(IF:6.393) 3.Que,H.#,Yan,X.#,Guo,B.#,Ma,H.,Wang,T.,Liu,P.,Gan,X.,Yan,Y.*,2019.TerminalDeoxynucleotidylTransferaseandRollingCircleAmplificationInducedG-triplexFormation:ALabel-freeFluorescentStrategyforDNAMethyltransferaseActivityAssay.SensorsandActuatorsB:Chemical291,394-400.(IF:6.393) 4.Fan,L.#,Yan,Y.#,Guo,B.,Zhao,M.,Li,J.,Bian,X.,Wu,H.,Cheng,W.,Ding,S.*,2019.Trimetallichybridnanodendritesandmagneticnanocomposites-basedelectrochemicalimmunosensorforultrasensitivedetectionofserumhumanepididymisprotein4.SensorsandActuatorsB:Chemical296.(IF:6.393) 5.Han,D.#,Yan,Y.#,Wang,J.,Zhao,M.,Duan,X.,Kong,L.,Wu,H.,Cheng,W.,Min,X.,Ding,S.*,2019.Anenzyme-freeelectrochemiluminesceaptasensorfortherapiddetectionofStaphylococcusaureusbythequenchingeffectofMoS2-PtNPs-vancomycintoS2O82-/O2system.SensorsandActuatorsB:Chemical288,586-593.(IF:6.393) 6.Ma,H.#,Guo,B.#,Yan,X.,Wang,T.,Que,H.,Gan,X.,Liu,P.,Yan,Y.*,2019.AsmartfluorescentbiosensorforthehighlysensitivedetectionofBRCA1basedona3DDNAwalkerandESDRcascadeamplification.RSCAdvances9(34),19347-19353.(IF:3.049) 2018年 1.Yan,X.#,Tang,M.#,Yang,J.,Diao,W.,Ma,H.,Cheng,W.,Que,H.,Wang,T.,Yan,Y.*,2018.Aone-stepfluorescentbiosensingstrategyforhighlysensitivedetectionofHIV-relatedDNAbasedonstranddisplacementamplificationandDNAzymes.RSCAdvances8(55),31710-31716. 2.Cheng,W.#,Zhang,Y.#,Yu,H.,Diao,W.,Mo,F.,Wen,B.,Cheng,W.,Yan,Y.*,2018.Anenzyme-freecolorimetricbiosensingstrategyforultrasensitiveandspecificdetectionofmicroRNAbasedonmismatchedstackingcircuits.SensorsandActuatorsB:Chemical255,3298-3304. 3.Diao,W.#,Tang,M.#,Ding,S.,Li,X.,Cheng,W.,Mo,F.,Yan,X.,Ma,H.,Yan,Y.*,2018.HighlysensitivesurfaceplasmonresonancebiosensorforthedetectionofHIV-relatedDNAbasedondynamicandstructuralDNAnanodevices.BiosensBioelectron100,228-234. 2016年 1.Diao,W.#,Tang,M.#,Ding,X.,Zhang,Y.,Yang,J.,Cheng,W.,Mo,F.,Wen,B.,Xu,L.,Yan,Y.*,2016.ElectrochemicalDNAbiosensorbasedonMNAzyme-mediatedsignalamplification.MicrochimicaActa183(9),2563-2569. 2.Yang,J.#,Tang,M.#,Diao,W.,Cheng,W.,Zhang,Y.,Yan,Y.*,2016.ElectrochemicalstrategyforultrasensitivedetectionofmicroRNAbasedonMNAzyme-mediatedrollingcircleamplificationonagoldelectrode.MicrochimicaActa183(11),3061-3067. 3.Yan,Y.,Ding,S.,Zhao,D.,Yuan,R.,Zhang,Y.,Cheng,W.*,2016.DirectultrasensitiveelectrochemicalbiosensingofpathogenicDNAusinghomogeneoustarget-initiatedtranscriptionamplification.SciRep6,18810. 4.Li,J.,Lei,P.,Ding,S.,Zhang,Y.,Yang,J.,Cheng,Q.,Yan,Y.*,2016.Anenzyme-freesurfaceplasmonresonancebiosensorforreal-timedetectingmicroRNAbasedonallostericeffectofmismatchedcatalytichairpinassembly.BiosensBioelectron77,435-441. 5.Li,D.,Cheng,W.,Yan,Y.,Zhang,Y.,Yin,Y.,Ju,H.*,Ding,S.*,2016.AcolorimetricbiosensorfordetectionofattomolarmicroRNAwithafunctionalnucleicacid-basedamplificationmachine.Talanta146,470-476. 6.Yuan,R.,Ding,S.,Yan,Y.,Zhang,Y.,Zhang,Y.,Cheng,W.*,2016.AfacileandpragmaticelectrochemicalbiosensingstrategyforultrasensitivedetectionofDNAinrealsamplebasedondefectiveTjunctioninducedtranscriptionamplification.BiosensBioelectron77,19-25. 2015年 1.Wu,J.#,Yan,Y.#,Li,S.,Ding,X.,Ding,S.*,Huang,Y.*,Monitoringofpatientglucoseinfusionusingasurfaceplasmonresonance-basedfiberopticsensor.MeasurementScience&Technology,2015,26(10). 2.Chen,W.#,Yan,Y.#,Zhang,Y.,Zhang,X.,Yin,Y.*,Ding,S.*,2015.DNAtransducer-triggeredsignalswitchforvisualcolorimetricbioanalysis.SciRep5,11190. 3.Li,S.#,Yan,Y.#,Zhong,L.,Liu,P.,Sang,Y.,Cheng,W.,Ding,S.*,2015.Electrochemicalsandwichimmunoassayforthepeptidehormoneprolactinusinganelectrodemodifiedwithgraphene,singlewalledcarbonnanotubesandantibody-coatedgoldnanoparticles.MicrochimicaActa182(11-12),1917-1924. 4.Tao,C.#,Yan,Y.#,Xiang,H.,Zhu,D.,Cheng,W.,Ju,H.,Ding,S.*,2015.AnewmodeforhighlysensitiveandspecificdetectionofDNAbasedonexonucleaseIII-assistedtargetrecyclingamplificationandmismatchedcatalytichairpinassembly.ChemCommun(Camb)51(20),4220-4222. 5.Zhang,Y.#,Yan,Y.#,Chen,W.,Cheng,W.,Li,S.,Ding,X.,Li,D.,Wang,H.,Ju,H.,Ding,S.*,2015.AsimpleelectrochemicalbiosensorforhighlysensitiveandspecificdetectionofmicroRNAbasedonmismatchedcatalytichairpinassembly.BiosensBioelectron68,343-349. 6.Yan,Y.,Shen,B.,Wang,H.,Sun,X.,Cheng,W.,Zhao,H.,Ju,H.,Ding,S.*,2015.AnovelandversatilenanomachineforultrasensitiveandspecificdetectionofmicroRNAsbasedonmolecularbeaconinitiatedstranddisplacementamplificationcoupledwithcatalytichairpinassemblywithDNAzymeformation.Analyst140(16),5469-5474. 7.Ding,X.#,Yan,Y.#,Li,S.,Zhang,Y.,Cheng,W.,Cheng,Q.,Ding,S.*,2015.SurfaceplasmonresonancebiosensorforhighlysensitivedetectionofmicroRNAbasedonDNAsuper-sandwichassembliesandstreptavidinsignalamplification.AnalChimActa874,59-65. 2014年 1.Lei,P.#,Tang,H.#,Ding,S.,Ding,X.,Zhu,D.,Shen,B.,Cheng,Q.,Yan,Y.*,2014.DeterminationoftheinvAgeneofSalmonellausingsurfaceplasmonresonancealongwithstreptavidinaptameramplification.MicrochimicaActa182(1-2),289-296. 2.Li,Y.#,Yan,Y.#,Lei,Y.,Zhao,D.,Yuan,T.,Zhang,D.,Cheng,W.,Ding,S.*,2014.Surfaceplasmonresonancebiosensorforlabel-freeandhighlysensitivedetectionofpointmutationusingpolymerizationextensionreaction.ColloidsSurfBBiointerfaces120,15-20. 3.Zhu,D.#,Yan,Y.#,Lei,P.,Shen,B.,Cheng,W.,Ju,H.,Ding,S.*,2014.AnovelelectrochemicalsensingstrategyforrapidandultrasensitivedetectionofSalmonellabyrollingcircleamplificationandDNA-AuNPsprobe.AnalChimActa846,44-50. 4.Shen,B.#,Li,J.#,Cheng,W.,Yan,Y.,Tang,R.,Li,Y.,Ju,H.,Ding,S.*,2014.Electrochemicalaptasensorforhighlysensitivedeterminationofcocaineusingasupramolecularaptamerandrollingcircleamplification.MicrochimicaActa182(1-2),361-367. 5.Cheng,W.,Zhang,W.,Yan,Y.,Shen,B.,Zhu,D.,Lei,P.,Ding,S.*,2014.AnovelelectrochemicalbiosensorforultrasensitiveandspecificdetectionofDNAbasedonmolecularbeaconmediatedcircularstranddisplacementandrollingcircleamplification.BiosensBioelectron62,274-279. 2013年 1.Yan,Y.,Zhao,D.,Yuan,T.,Hu,J.,Zhang,D.,Cheng,W.,Zhang,W.,Ding,S.*,2013.ASimpleandHighlySensitiveElectrochemicalBiosensorformicroRNADetectionUsingTarget-AssistedIsothermalExponentialAmplificationReaction.Electroanalysis,25(10),2354-2359. 2.Zhang,D.#,Yan,Y.#,Cheng,W.,Zhang,W.,Li,Y.,Ju,H.,Ding,S.*,2013.Streptavidin-enhancedsurfaceplasmonresonancebiosensorforhighlysensitiveandspecificdetectionofmicroRNA.MicrochimicaActa180(5-6),397-403. 2012年 1.Zhang,D.#,Yan,Y.#,Li,Q.,Yu,T.,Cheng,W.,Wang,L.,Ju,H.,Ding,S.*,2012.Label-freeandhigh-sensitivedetectionofSalmonellausingasurfaceplasmonresonanceDNA-basedbiosensor.JBiotechnol160(3-4),123-128 著作: 1.提高《化学分析与仪器分析》教学质量的探讨,重庆医学,2017,46(3):419-420。 2.研究生《蛋白质组学》样品制备与分离的教学探索,中华医学教育探索杂志,2012,11(12):1254-125

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