个人简介
学习(进修)与工作经历
1987.9~1991.7获西南师范大学化学系理学学士学位;
1991.9~1994.7获北京师范大学有机化学专业理学硕士学位;
2003.9~2006.7获重庆医科大学药理学专业博士学位;
1994.7~2003.07,重庆医科大学基础医学院化学教研室任教;
2003.07~2010.12,重庆医科大学药学院药物化学教研室任教,
2011.1~至今,重庆医科大学检验医学院、临床检验诊断学教育部重点实验室。
1996.07,讲师,2006.07,副教授,2011.07,教授,硕士生导师
研究领域
蛋白质标记、修饰与位点选择性固定化;亲和分离分析材料与应用;磁分离选择性指数富集快速筛选组合合成配体混合物、中药方剂及天然产物混合物中高亲和力配体
知识产权
1.杨晓兰,何姝,廖飞,胡小蕾,张祥,陈春艳。异香兰酸衍生物与饱和小环胺缩合所得苯甲酰胺类人PDE4B抑制剂。中国发明专利,ZL201610093456.6,授权公告号CN105646399B,授权日期2019-03-08
2.廖飞,杨晓兰,胡小蕾,冯一然,种慧敏,李雨薇。一种均相定量比较未纯化酶及其突变体的方法,2018.08.10,国家发明专利
3.廖飞,杨晓兰,张纯,刘红博,高昂。在竞争性抑制剂保护下用针对氨基的活化聚乙二醇修饰尿酸酶的方法,2018.07.06,国家发明专利
4.廖飞,杨晓兰,胡小蕾,许榜田,王昕,陈春艳,秦家林,谭登,黄亚。双-对烷氧基苯丙烯酮类谷胱甘肽-S-转移酶潜抑制剂,2018.03.27,国家发明专利,第二发明人。
5.LiaoFei,YangXiaolan,LiuHongbo,Dangjizheng,LongGaobo,LiYuanli,LiuLin.Methodforsimultaneouslymeasuringactivityofvariousenzymesbyusingmultiple-wavelengthabsorptionsinglechannel.US9611502B2,Dateofpatent,April4,2017.
6.廖飞,杨晓兰,胡小蕾,秦家林,何琛雄,李元丽,刘霖。一种荧光滴定测定单抗活性位点浓度的方法。中国发明专利,ZL201410190645.6.授权公告号:CN103954599B,日期2016-6-8
7.廖飞,杨晓兰,胡小蕾,李元丽,陈春艳,刘霖。一种快速比较未纯化融合酶及其突变体比活性的方法。中国发明专利,ZL201410190677.6.授权公告号:CN103954762B,日期2016-5-30
8.廖飞,杨晓兰,胡小蕾,黄亚,陈远翔,李元丽。一种双试剂法测定尿酸含量试剂盒的配方。中国发明专利,ZL201310591044.1;授权公告号CN103571916B,日期2016-03-16.
9.廖飞,杨晓兰,党纪政,刘红博,张奕,李元丽。一种用4-硝基-1-萘酚或其衍生物为显色团制备b-半乳糖苷酶显色底物的方法及其应用。中国发明专利,ZL201210461131.0。授权公告号:CN102977160B,日期2016-01-06.
10.廖飞,杨晓兰,刘红博,党济政,龙高波,李元丽,刘霖。用多波长吸收单通道同步测量多种酶活性的方法。中国发明专利,ZL201210355606.8;授权公告号:CN102879343B,日期2014-10-29
11.廖飞,杨晓兰,龙高波,蒲军,刘红博,李元丽。用两端带羧基的直链亲水聚合物包被纳米磁芯制备微纳米磁性材料的方法及其应用。中国发明专利,ZL201210046309.5.授权公告号:CN102617810B,日期2014-09-24.IPC分类号C08F290/06(2006.01)I
12.廖飞,杨晓兰,龙高波,刘红博,廖娟。联用酶反应动力学过程分析法和终点平衡法测定酶底物量的方法。中国发明专利,ZL201110321126.5.授权公告号:CN102507470B,日期2014-09-24.IPC分类号G01N21/31(2006.01)I
13.廖飞,杨晓兰,袁拥华,赵运胜,冯娟,张纯,谢燕玲。作用于血液中低分子物质的大分子药物的体外给药装置及其应用。中国发明专利,ZL200910251060.X;授权公告号:CN102107032B,日期2014-08-27.IPC分类号A61M1/36(2006.01)I
14.廖飞,赵运胜,杨晓兰,桑宇,谢艳玲,廖娟,刘北忠,于明安。用偶联酶的最大瞬时速度测定靶酶活性的方法。中国发明专利ZL200910104280.X,授权公告号:CN101597636B,日期2012-10-31.IPC分类号C12Q1/00(2006.01)I
15.廖飞,杨晓兰,谢燕玲,邓萍,赵运胜,朱莎,甘志勇,廖娟,刘冬,于明安。联用磁分离与色谱分析测定混合物中配体亲和力的方法。中国发明专利,ZL200810237298.2,授权公告号:CN101620205B,日期2012-09-05.IPC分类号G01N30/00(2006.01)I
16.廖飞,赵运胜,杨晓兰,李想,张纯,冯娟,卜友泉,谢燕玲。一种重组表达苛求芽孢杆菌胞内尿酸酶及其聚乙二醇修饰与应用。中国发明专利,ZL200910103741.1,授权公告号:CN101875922B,日期2012-02-22.IPC分类号C12N9/06(2006.01)I
课题名称(下达编号)
混合物中低丰度高亲和力配体的指数富集技术及应用”,编号31570862
科技部863IVD领域重大专项(预研)项目“新型免疫检测技术的研究”编号2011AA02A108;子课题6:“新型试剂材料制备”
指数富集快速发现天然产物混合物中抗结核活性先导配体,编号cstc2019jcyj-msxmX0166
融合表达标签GST高亲和力配体的设计筛选与应用(CSTC2012JJA0057)
近期论文
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1.Fluorometrictitrationapproachforcalibrationofquantityofbindingsiteofpurifiedmonoclonalantibodyrecognizingepitope/haptennonfluorescentat340nm.第一作者.AnalChem.1区,IF5.636.2014Jun17;86(12):5667-5672.doi:10.1021/ac501111h
2.Spectrophotometric-dual-enzyme-simultaneous-assayinonereactionsolution(SDESA):chemometricsandexperimentalmodels.二人共同第一作者.AnalChem.1区,IF5.825.2013Feb,85(4):2143-2154.doi.:10.1021/ac302786p
3.Ampholyticion-exchangematerialscoatedwithsmallzwitterionsforhigh-efficacypurificationofionizablesolublebiomacromolecules.二人共同通讯作者.IntJBiolMacromol.1区,IF4.784.2018Aug6(Epub),120(PtB):2234–224.doi:10.1016/j.ijbiomac.2018.08.020
4.Extracorporealdeliveryofatherapeuticenzyme.指导研究生论文.三人共同第一作者SciRep.1区.IF4.259.2016Aug1;6:30888.doi:10.1038/srep30888
5.Resonant-Mie-scatteringofaggregatesofphosphomolybdateandpapaverineformeasuringactivitiesandscreeninginhibitorsofcyclicnucleotidephosphodiesteraseisozymes.指导研究生论文.二人共同第一作者AnalChimActa1区.IF4.517.2013Dec,804:215-220.doi:10.1016/j.aca.2013.10.023
6.Comparisonofthefull-Lengthand152~528TruncateofhumanCyclicnucleotidephosphodiesterase4B2forthecharacterizationofInhibitors.二人共同通讯作者CombChemHighThroughputScreen.3区.IF1.503.2019;22(1):49-58.DOI:10.2174/1386207322666190306142810
7.GlutathioneS_x005f_x001e_transferaseisozymealpha1ispredominantlyinvolvedinthecisplatinresistanceofcommontypesofsolidcancer.二人共同通讯作者.OncologyReports.3区.IF3.041.2019Feb,41(2):989-998.doi:10.3892/or.2018.6861
8.Anovelstrategyforinvivoangiogenesisandosteogenesis:magneticmicro-movementinabonescaffold.第二作者.ArtifCellNanomedB1区.IF4.462.2018July22:1-10.doi:10.1080/21691401.2018.1465947
9.High-throughputestimationofspecificactivitiesofenzyme/mutantsincelllysatesthroughimmunoturbidimetricassayofproteins.第一作者.AnalBiochem.3区.IF2.275.2017,534:91-98.doi:10.1016/j.ab.2017.05.015
10.PolyclonalantibodiesinmicroplatestopredictthemaximumAdsorptionactivitiesofenzyme/mutantsfromcelllysates.二人共同第一作者.ProteinJ.4区.IF1.133.2017Jun;36(3):212-219.doi:10.1007/s10930-017-9716-z
11.Apracticalsystemforhigh-throughputscreeningofmutantsofBacillusfastidiosusUricase.二人共同第一作者.ApplBiochemBiotechnol.3区.IF1.797.2017Feb;181(2):667-681.doi:10.1007/s12010-016-2240-3
12.Achievementoflinearresponseforcompetitivebioaffinityassayofligand:criteriaofoptimizedinteractionsystem.二人共同通讯作者.RSCAdv.2区.IF3.108.2016,6(112):110858-110865.DOI:10.1039/c6ra06426d
13.AnumericalapproachforkineticanalysisofthenonexponentialthermoinactivationprocessofUricase.指导研究生论文.ProteinJ.4区.IF1.139.2016Aug;35(4):318-329.doi:10.1007/s10930-016-9675-9.
14.StrikingEffectsofstoragebuffersonapparentHalf-LivesoftheactivityofPseudomonasaeruginosaArylsulfatase.二人共同第一作者.ProteinJ.4区.IF1.139.2016Aug;35(4):283-290.doi:10.1007/s10930-016-9671-0.
15.FacilealkalinelysisofEscherichiacolicellsinhigh-throughputmodeforscreeningenzymemutants:arylsulfataseasanexample.二人共同第一作者.ApplBiochemBiotechnol.3区.IF1.751.2016Jun;179(4):545-557.doi:10.1007/s12010-016-2012-0
16.Comparisonoftheimmobilizationof6His-taggedproteinsonmagnetic-submicron-particlefunctionalizedwithNi2+-NTAandbis-sulfone.第一作者.NanosciNanotechnLett.4区.IF1.007.2015Jun,7(6):484-494doi:10.1166/nnl.2015.1996
17.Fluorometrictitrationassayofaffinityoftight-bindingnonfluorescentinhibitorofglutathiones-transferase.指导研究生论文.JFluoresc3区,IF1.601.2015Jan;25(1):1-8.doi:10.1007/s10895-014-1475-z
18.ApproximatedmaximumadsorptionofHis-taggedenzyme/mutantsonNi2+-NTAforcomparisonofspecificactivities.三人共同第一作者.IntJBiolMacromol.1区.IF3.138.2015Mar;74:211-217.doi:10.1016/j.ijbiomac.2014.12.009.
19.Comparisonofcandidatepairsofhydrolyticenzymesforspectrophotometric-dual-enzyme-simultaneous-assay.三人共同第一作者.AnalSci.3区.IF1.174.2015May,31(5):421-427.doi:10.2116/analsci.31.421.
20.Core–shellbiopolymermicrospheresforsustaineddrugrelease.三人共同第一作者.JApplPolymSci.2区.IF1.866.2015April;132(14):41782.DOI:10.1002/app.41782
21.OptimizationofpHvaluestoformulatethebi-reagentkitforserumuricacidassay三人共同第一作者.BiotechnolApplBioc4区.IF1.429.2015Jan-Feb;62(1):137-144.doi:10.1002/bab.1227.
22.CrystalstructureofBacillusfastidiousuricaserevealsanunexpectedfoldingoftheC-terminusresiduescrucialforthermostabilityunderphysiologicalconditions.第二作者.ApplMicriobiolBiot2区.IF3.376.2015Oct;99(19):7973-7986.doi:10.1007/s00253-015-6520-6
23.Facilequantitativecomparisonofspecificactivitiesoffusion-taggedenzyme/mutantsincelllysatesviapredictionoftheirmaximumadsorptionbyanti-tagantibodyimmobilizedinmicroplatewells.二人共同第一作者.RSCAdv.1区.IF3.840.2014Jun;4(56):29925-29932.DOI:10.1039/c4ra03189j
24.ComparisonofmodificationofabacterialuricasewithN-hydroxysuccinimideestersofsuccinateandcarbonateofmonomethoxylpoly(ethyleneglycol).指导研究生论文.BiotechnolApplBioc.4区.IF1.362.2014Nov-Dec;61(6):683-690.DOI:10.1002/bab.1215
25.Selectiveandsensitivehomogenousassayofserumalbuminwith1-anilinonaphthalene-8-sulphonateasabiosensor.第二作者.AnalChimActa.1区.IF4.513.2014,829(1):60-67.doi:10.1016/j.aca.2014.04.047
26.Microplate-basedmethodtoscreeninhibitorsofisozymesofcyclicnucleotidephosphodiesterasefusedtoSUMO.第二作者.JEnzymInhibMedCh.3区.IF2.332.2014Dec;29(6):836-839.doi:10.3109/14756366.2013.858145
27.Classificationofdifferencebetweeninhibitionconstantsofaninhibitortofacilitateidentifyingtheinhibitiontype.第一作者.JEnzymeInhibitMedChem3区.IF2.383.2013Feb;28(1):205-213.doi:10.3109/14756366.2011.645240
28.Facileone-stepcoatingapproachtomagneticsubmicronparticleswithpoly(ethyleneglycol)coatsandabundantaccessiblecarboxylgroups.二人共同第一作者.InterJNanomed.1区.IF4.195.2013Feb25(Epub),8:791-807.doi:10.2147/ijn.s41411
29.Chromogenicsubstratefrom4-nitro-1-naphtholforhydrolyticenzymeofneutralorslightlyacidicoptimumpH:4-nitro-1-naphthyl-β-D-galactopyranosideasanexample.指导研究生论文.BioorgMedChemLett.3区.IF2.331.2013,23(3):646-649.doi:10.1016/j.bmcl.2012.11.120
30.ComparisonofF?rster-resonance-energy-transferacceptorsfortryptophanandtyrosineresiduesinnativeproteinsasdonors.指导研究生论文.JFluorescence.4区.IF1.667.2013,23(1):147-157.doi:10.1007/s10895-012-1128-z
31.Twoglycosidasesaslabelenzymesforconcurrentenzyme-linked-immunosorbent-assayoftwocomponentsviaspectrophotometric-dual-enzymesimultaneous-assayinonesolution.二人共同第一作者.AnalMethods.2区.IF1.938.2013Aug;5(10):5969-5976.doi:10.1039/c3ay40615f
32.Comparisonofactivityindexesforrecognizingenzymemutantsofhigheractivitywithuricaseasmodel.三人共同第一作者.ChemCentJ.2区.IF1.663.2013,7:69.doi:10.1186/1752-153X-7-69
33.Facilecharacterizationoftheimmobilizationofstreptavidinonmagneticsubmicronparticleswithafluorescentprobeofstreptavidin.三人共同第一作者.ApplSpectros.1区.IF2.014.2013Jun;67(6):688-691.doi:10.1366/12-06963
34.MethodtoscreenaromaticligandsinmixturesforquantitativeaffinitiestotargetusingmagneticseparationofboundligandsalongwithHPLCandUVphotometrydetection.第一作者.MicrochimActa1区.IF3.434.2012,Jan;176(1):243-249.DOI10.1007/s00604-011-0696-y
35.FacilespectrophotometricassayofmolarequivalentsofN-hydroxysuccinimideestersofmonomethoxylpoly-(ethyleneglycol)derivatives.二人共同第一作者.ChemCentJ.3区.IF1.312.2012Nov23(Epub);6(1):142.doi:10.1186/1752-153X-6-142
36.Site-specificPEGylationoftherapeuticproteinsviaoptimizationofbothaccessiblereactiveaminoacidresiduesandPEGderivatives.指导研究生论文Biodrugs.3区.IF2.809.2012Aug1;26(4):209-215.doi:10.1007/bf03261880
37.Uricasesastherapeuticagentstotreatrefractorygout:currentstatesandfuturedirections.第一作者.DrugDevRes.4区.IF0.869.2012Mar1;73(2):66-72.DOI:10.1002/ddr.20493
38.Integrationofkineticanalysisofreactioncurvewithaproperclassicalapproachforenzymaticanalysis.第一作者ScientificWorldJournal4区.IF1.730.2012May(Epub),2012:969767.doi:10.1100/2012/969767
39.Estimationofaffinitiesofligandsinmixturesviamagneticrecoveryoftarget-ligandcomplexesandchromatographicanalyses:chemometricsandanexperimentalmodel.BMCBiotechnol.2011,11:44第一作者
40.Kineticanalysisoflactate-dehydrogenase-coupledreactionprocessandmeasurementofalaninetransaminasebyanintegrationstrategy.AnalSci.2010,26(11):1193-1198.二人共同第一作者
41.Homogeneouscompetitiveassayofligandaffinitiesbasedonquenchingfluorescenceoftyrosine/tryptophanresiduesinaproteinviaFOrster-resonance-energy-transfer.SpectrochimActaAMolBiomolSpectrosc.2010,77(4):869-876.二人共同第一作者
42.Effectsofmodificationofaminogroupswithpoly(ethyleneglycol)onarecombinanturicasefromBacillusfastidiosus.BiosciBiotechnolBiochem2010,74(6):1298-1301.指导研究生论文
著作教材
1.XiaolanYang,GaoboLong,HuaZhaoandFeiLiao*.KineticAnalysesofEnzymeReactionCurveswithNewIntegratedRateEquationsandApplications,inChemometricsinPracticalApplications(2012).pp.157-184.KurtVarmuza(Ed.),ISBN:978-953-51-0438-4,InTech