袁必锋 - 武汉大学 - 化学与分子科学学院

个人简介

1997.9-2001.6 武汉大学生命科学学院生物化学专业学士 2001.9-2006.6 武汉大学生命科学学院生物物理专业博士 2006.11-2007.6 新加坡国立大学生物系博士后 2007.7-2010.12 美国加州大学河滨分校化学系博士后 2011.1 至今武汉大学化学与分子科学学院教授/博导袁必锋:博士，武汉大学化学与分子科学学院教授、博士生导师，国家自然科学基金优秀青年基金获得者（2015年）。2001年和2006年于武汉大学生命科学学院分别获得学士和博士学位。2006年至2010年在新加坡国立大学和加州大学河滨分校开展博士后研究。2011年任武汉大学化学与分子科学学院教授、博士生导师。担任Chinese Chemical Letters副主编、Chemical Research in Toxicology顾问编委(Editorial Advisory Board)、Scientific Reports编委、《色谱》编委、《分析测试学报》青年编委、《高等学校化学学报》青年编委、中国生物物理学会代谢组学分会理事。主要研究方向为生物分析化学、核酸化学生物学。在所从事的研究工作中，建立了DNA、RNA、蛋白质等多种生物大分子修饰（表观遗传修饰）的高灵敏分析方法，开展了生物大分子修饰和疾病的相关性研究；建立了多种代谢组学分析方法，开展了基于代谢组的临床疾病标志物分析。在PNAS、Nucleic Acids Research、Chemical Science等学术刊物上发表了190余篇SCI收录论文，专著一部，论文引用七千余次。

研究领域

1. 核酸表观遗传修饰核酸表观遗传修饰不仅在基因调控等方面有重要作用，而且在癌症等许多疾病的发生中也扮演着重要角色。本课题组通过建立质谱分析和测序等研究方法，对核酸表观遗传修饰的生理功能进行研究。 2. 代谢组学发展基于质谱技术的代谢组学研究方法，结合相应的生物学手段，探索代谢物在疾病发生、发展过程中的生理功能。 3. 核酸损伤和修复发展核酸损伤的检测手段，探索核酸损伤的定位、复制、转录、修复机制。

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Ma, C.J., Li, L., Shao, W.X., Ding, J.H., Cai, X., Lun, Z., Yuan, B.F.* and Feng, Y.Q. (2021) An enzyme-mediated bioorthogonal labeling method for genome-wide mapping of 5-hydroxymethyluracil. Chem Sci, 12, 14126-14132. Feng, Y., Chen, J.J., Xie, N.B., Ding, J.H., You, X.J., Tao, W.B., Zhang, X., Yi, C., Zhou, X., Yuan, B.F.* and Feng, Y.Q. (2021) Direct decarboxylation of Ten-eleven translocation-produced 5-carboxylcytosine in mammalian genomes forms a new mechanism for active DNA demethylation. Chem Sci, 12, 11322-11329. Cheng, M.Y., You, X.J., Ding, J.H., Dai, Y., Chen, M.Y., Yuan, B.F.* and Feng, Y.Q. (2021) Novel dual methylation of cytidines in RNA of mammals. Chem Sci, 12, 8149–8156. Dai, Y., Yuan, B.F.* and Feng, Y.Q. (2021) Quantification and mapping of DNA modifications. RSC Chem Biol, 2, 1096–1114. Dai, Y., Qi, C.B., Feng, Y., Cheng, Q.Y., Liu, F.L., Cheng, M.Y., Yuan, B.F.* and Feng, Y.Q. (2021) Sensitive and Simultaneous Determination of Uridine Thiolation and Hydroxylation Modifications in Eukaryotic RNA by Derivatization Coupled with Mass Spectrometry Analysis. Anal Chem. 93, 6938-6946. Liu, F.L., Ye, T.T., Ding, J.H., Yin, X.M., Yang, X.K., Huang, W.H., Yuan, B.F.* and Feng, Y.Q. (2021) Chemical Tagging Assisted Mass Spectrometry Analysis Enables Sensitive Determination of Phosphorylated Compounds in a Single Cell. Anal Chem, 93, 6848-6856. Feng, Y., Xie, N.B., Tao, W.B., Ding, J.H., You, X.J., Ma, C.J., Zhang, X., Yi, C., Zhou, X., Yuan, B.F.* and Feng, Y.Q. (2020) Transformation of 5-Carboxylcytosine to Cytosine Through C–C Bond Cleavage in Human Cells Constitutes a Novel Pathway for DNA Demethylation. CCS Chem, 2, 994-1008. Cheng, Q.Y., Xiong, J., Ma, C.J., Dai, Y., Ding, J.H., Liu, F.L., Yuan, B.F.* and Feng, Y. Q. (2020) Chemical tagging for sensitive determination of uridine modifications in RNA, Chem Sci, 11, 1878-1891. Ding, J.H., Ma, C.J., Chen, M.Y., Chen, B., Yuan, B.F.* and Feng, Y.Q. (2020) Quantification and single-base resolution analysis of N1-methyladenosine in mRNA by ligation-assisted differentiation. Anal Chem, 92, 2612-2619. Liu, F.L, Qi, C.B., Cheng, Q.Y., Ding, J.H., Yuan, B.F.* and Feng, Y.Q. (2020) Diazo reagent labeling with mass spectrometry analysis for sensitive determination of ribonucleotides in living organisms. Anal Chem, 92, 2301-2309. You, X.J., Liu, T., Ma, C.J., Qi, C.B., Tong, Y., Zhao, X., Yuan, B.F.* and Feng, Y.Q. (2019) Determination of RNA hydroxylmethylation in mammals by mass spectrometry analysis. Anal Chem, 91, 10477-10483. Tang, F., Liu, S., Li, Q.Y., Yuan, J., Li, L., Wang, Y., Yuan, B.F.* and Feng, Y.Q. (2019) Location analysis of 8-oxo-7,8-dihydroguanine in DNA by polymerase-mediated differential coding. Chem Sci, 10, 4272–4281. Xiong, J., Ye, T.T., Ma, C.J., Cheng, Q.Y., Yuan, B.F.* and Feng, Y.Q. (2019) N6-Hydroxymethyladenine: a hydroxylation derivative of N6-methyladenine in genomic DNA of mammals. Nucleic Acids Res, 47, 1268-1277. Chen, B., Yuan, B.F.* and Feng, Y.Q. (2019) Analytical Methods for Deciphering RNA Modifications. Anal Chem, 91, 743-756. Xu, J., Zhang, Q.F., Zheng, J., Yuan, B.F.* and Feng, Y.Q.* (2019) Mass spectrometry-based fecal metabolome analysis. TrAC-Trend Anal Chem, 112, 161-174. Ma, C.J., Ding, J.H., Ye, T.T., Yuan, B.F.* and Feng, Y.Q. (2019) AlkB Homologue 1 Demethylates N(3)-Methylcytidine in mRNA of Mammals. ACS Chem Biol, 14, 1418-1425. Li, Q.Y., Xie, N.B., Xiong, J., Yuan, B.F.* and Feng, Y.Q. (2018) Single-Nucleotide Resolution Analysis of 5-Hydroxymethylcytosine in DNA by Enzyme-Mediated Deamination in Combination with Sequencing. Anal Chem, 90, 14622-14628. Chu, J.M., Ye, T.T., Ma, C.J., Lan, M.D., Liu, T., Yuan, B.F.* and Feng, Y.Q. (2018) Existence of Internal N7-Methylguanosine Modification in mRNA Determined by Differential Enzyme Treatment Coupled with Mass Spectrometry Analysis. ACS Chem Biol. 13, 3243−3250 Jiang, H.P., Xiong, J., Liu, F.L., Ma, C.J., Tang, X.L., Yuan, B.F.* and Feng, Y.Q. (2018) Modified nucleoside triphosphates exist in mammals. Chem Sci, 9, 4160-4167. Yuan, B.F., Zhu, Q.F., Guo, N., Zheng, S.J., Wang, Y.L., Wang, J., Xu, J., Liu, S.J., He, K., Hu, T., Zheng, Y.W., Xu, F.Q. and Feng, Y.Q.* (2018) Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis. Anal Chem, 90, 3512-3520.