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Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability
Guo Xiao,Jiang Luying,An Yajing,Lu Fuping,Liu Fufeng,Wang Bo
Page:110255 ISSN:0141-0229 2023
Container-title:Enzyme and Microbial Technology
Efficient Secretory Production of Lytic Polysaccharide Monooxygenase BaLPMO10 and Its Application in Plant Biomass Conversion
Xiao Guo ,Yajing An ,Fuping Lu ,Fufeng Liu and Bo Wang
Int. J. Mol. Sci. 2023, 24(11), 9710; https://doi.org/10.3390/ijms24119710
[Engineering the plastic degradation enzyme Ple629 from marine consortium to improve its thermal stability]
[Article in Chinese]
Yipei Zhao , Hao Wang , Pan Wu , Zhishuai Li , Fufeng Liu , Qun Gu , Weidong Liu , Jian Gao , Xu Han
PMID: 37212230，2023 May 25;39(5):2040-2052. doi: 10.13345/j.cjb.221045.
Identification of Binding Sites in the Nucleotide-Binding Domain of P-Glycoprotein for a Potent and Nontoxic Modulator, the Amine-Containing Monomeric Flavonoid FM04
Zhen Liu, Iris L. K. Wong, Jingcheng Sang, Fufeng Liu, Clare S. W. Yan, Jason W. Y. Kan, Tak Hang Chan*, and Larry M. C. Chow*
Cite this: J. Med. Chem. 2023, 66, 9, 6160–6183
Publication Date:April 25, 2023
https://doi.org/10.1021/acs.jmedchem.2c02005
Cross-linked enzyme aggregates immobilization: preparation, characterization, and applications
Ning Chen,Baogen Chang,Nian Shi,Wenxing Yan,Fuping Lu &Fufeng Liu
Pages 369-383 | Received 24 Aug 2021, Accepted 12 Jan 2022, Published online: 17 Apr 2022
https://doi.org/10.1080/07388551.2022.2038073
Discrimination of psychrophilic enzymes using machine learning algorithms with amino acid composition descriptor
Huang Ailan,Lu Fuping,Liu Fufeng
ISSN:1664-302X 2023
Container-title:Frontiers in Microbiology
Robust and recyclable cross-linked enzyme aggregates of sucrose isomerase for isomaltulose production
Chen Ning,Chang Baogen,Shi Nian,Lu Fuping,Liu Fufeng
Page:134000 ISSN:0308-8146 2023
Container-title:Food Chemistry
Bionics design of affinity peptide inhibitors for SARS-CoV-2 RBD to block SARS-CoV-2 RBD-ACE2 interactions
Heliyon
2023-01 | Journal article
DOI: 10.1016/j.heliyon.2023.e12890
CONTRIBUTORS: Xiaofeng Liu; Luying Jiang; Li Li; Fuping Lu; Fufeng Liu
A Combined Computational–Experimental Study on the Substrate Binding and Reaction Mechanism of Salicylic Acid Decarboxylase
Catalysts
2022-12 | Journal article | Author
DOI: 10.3390/catal12121577
CONTRIBUTORS: chen fuqiang; Yipei Zhao; Chenghua Zhang; Wei Wang; Jian Gao; Qian Li; Huimin Qin; Yujie Dai; Weidong Liu; Fufeng LIU et al.
Luying Jiang, Quancheng Sun, Li Li, Fuping Lu, Fufeng Liu*, Molecular insights into inhibitory effect of GV971 components with different sizes against conformational transition of Aβ42 monomer, accepted.
Wenping Zhao#, Luying Jiang#, Wenjuan Wang, Jingcheng Sang, Quancheng Sun, Qinchen Dong, Li Li*, Fuping Lu*, Fufeng Liu*. Design of Carboxylated Single-Walled Carbon Nanotube as High-Efficient Inhibitor against Aβ40 Fibrillation based on HyBER Mechanism, Journal of Materials Chemistry B, 2021, 9 (34): 6902-6914.
Fenghua Wang#, Ying Wang#, Luying Jiang, Wenqian Wang, Jingcheng Sang, Xinyu Wang, Fuping Lu, Fufeng Liu*. The food additive fast green FCF inhibits α‑synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food & Function. 2021, 12: 5465.
Jialin Li, Luying Jiang; Xue Cao; Yifan Wu; Fuping Lu; Fufeng Liu*; Yu Li*; Yihan Liu*, Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation, Enzyme and Microbial Technology, 2021, 147: 109787.
Xiao Guo, Chengcheng Chai, Yajing An, Chong Peng, Nian Shi, Wenqian Wang, Fuping Lu, Yujie Dai*, Fufeng Liu*. Rational design of signal peptides for improved MtC1LPMO production in Bacillus amyloliquefaciens, International Journal of Biological Macromolecules, 2021, 175: 262-269.
陈贝贝，蒋露莹, 郭芳妍，屈莉莉，汪文倩，金成华*，刘夫锋*. 托卡朋衍生物PCDNA抑制Aβ42纤维化并降低其细胞毒性, 药学学报, 2021, 56(4): 1063-1069.
Fufeng Liu*, Wenjuan Wang, Zhenquan Xuan, Luying Jiang, Beibei Chen, Qinchen Dong, Fang Zhao, Wei Cui, Li Li,* Fuping Lu*. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice, International Journal of Biological Macromolecules, 2021, 170: 33-41.
Tao Li; Hongbin Wang; Jingwen Li; Luying Jiang; Hongwei Kang; Zehui Guo; Chen Wang; Wei Yang; Fufeng Liu*; Fuping Lu*; Yihan Liu*. Enzymatic characterization, molecular dynamics simulation, and application of a novel Bacillus licheniformis laccase, International Journal of Biological Macromolecules, 2021, 167: 1393-1405.
路福平, 黄爱岚, 赵蕾, 刘晓凤, 郭宵, 陈宁, 刘夫锋. 计算机模拟在食品工业用酶改造中的应用, 中国食品学报, 2020, 20(11): 1-10.
Lin Huang, Jieying Ma, Jingcheng Sang, Nan Wang, Shuang Wang, Chen Wang, Hongwei Kang, Fufeng Liu*, Fuping Lu*, Yihan Liu*. Enhancing the thermostability of phospholipase D from Streptomyces halstedii by directed evolution and elucidating the mechanism of a key amino acid residue using molecular dynamics simulation, International Journal of Biological Macromolecules, 2020, 164: 3065-3074.
Longgang Jia, Yuqing Liu, Wenliang Wang, Ying Wang, Haiqing Liu, Fufeng Liu, Rong Chen, Valina L. Dawson, Ted M. Dawson, Fuping Lu, Lei Liu*, Yanping Wang*, Xiaobo Mao*. Molecular mediating prion-like α-synuclein fibrillation from toxic PFFs to nontoxic species, ACS Applied Bio Materials, 2020, 3(9): 6096-6102.
Xiao Guo, Yajing An, Chengcheng Chai, Jingcheng Sang, Luying Jiang, Fuping Lu, Yujie Dai, Fufeng Liu*. Construction of the R17L mutant of MtC1LPMO for improved lignocellulosic biomass conversion by rational point mutation and investigation of the mechanism by molecular dynamics simulations, Bioresource Technology, 2020, 317: 124024.
Fufeng Liu#*, Wenping Zhao#, Fang Zhao, Qinchen Dong, Ying Wang, Wei Wei, Longgang Jia, Li Li, Fuping Lu*, Dual Effect of the Acidic Polysaccharose Ulvan on the Inhibition of Aβ Fibrillation and Disintegration of Mature Fibrils, ACS Applied Materials Interface, 2020, 12(37): 41167-41176. Top1区
Xiao Guo, Jingcheng Sang, Chengcheng Chai, Yajing An, Zhifeng Wei, Yujie Dai, Fuping Lu*, Fufeng Liu*. A lytic polysaccharide monooxygenase from Myceliophthora thermophila C1 and its characterization in cleavage of glycosidic chain of cellulose. Biochemical Engineering Journal, 2020, 162: 107712.
郭宵，安亚静，柴成程，路福平，刘夫锋*。大肠杆菌分泌表达裂解性多糖单加氧酶发酵条件的优化, 食品与发酵工业, 2020, 46(5): 31-37.
Jia, Longgang; Wang, Wenjuan; Yan, Yushan; Hu, Rui; Sang, Jingcheng; Zhao, Wenping; Wang, Ying; Wei, Wei; Cui, Wei; Yang, Guoqiang; Lu, Fuping*; Zheng, Jie*; Liu, Fufeng*. General Aggregation-Induced Emission Probes for Amyloid Inhibitors with Dual Inhibition Capacity against Amyloid beta-Protein and alpha-Synuclein, ACS Applied Materials Interface, 2020, 12 (28): 31182-31194.
郭宵，安亚静，柴成程，蒋露莹，路福平，刘夫锋٭，戴玉杰٭。来源于真菌的AA9家族裂解性多糖单加氧酶的研究进展，微生物学通报，2020, 47(7): 2309-2320.
Longgang Jia, Wenping Zhao, Wei Wei, Xiao Guo, Wenjuan Wang, Ying Wang, Jingcheng Sang, Fuping Lu*, Fufeng Liu*. Expression and purification of amyloid β-protein, tau and α-synuclein in Escherichia coli: A review. Critical Reviews in Biotechnology, 2020, 40(4): 475-489.
位薇, 常保根, 王英, 路福平,* 刘夫锋*。Tau蛋白核心片段306~378的异源表达、纯化及聚集特性验证。中国生物工程杂志, 2020, 40(5): 22-29.
Fufeng Liu*, Fang Zhao, Wenjuan Wang, Jingcheng Sang, Longgang Jia, Li Li, Fuping Lu*. Cyanidin-3-O-glucoside inhibits Aβ40 fibrillogenesis, disintegrates preformed fibrils, and reduces amyloid cytotoxicity. Food & Function, 2020, 11: 2573-2587.
贾龙刚，王英，路福平，刘夫锋*。α-突触核蛋白的高效表达纯化及表征。天津科技大学学报，2020, 35(2):1-5.
Longgang Jia, Wenping Zhao, Jingcheng Sang, Wenjuan Wang, Wei Wei, Ying Wang, Fang Zhao, Fuping Lu*, Fufeng Liu*. Inhibitory effect of a flavonoid dihydromyricetin against Aβ40 amyloidogenesis and its associated cytotoxicity, ACS Chemical Neuroscience, 2019, 10: 4696-4703.
Fufeng Liu*, Ying Wang, Jingcheng Sang, Wei Wei, Wenping Zhao, Beibei Chen, Fang Zhao, Longgang Jia, Fuping Lu*. Brazilin inhibits α-synuclein fibrillogenesis, disrupts mature fibrils and protects against amyloid-induced cytotoxicity, J Agric Food Chem, 2019, 67(42): 11769-11777. IF：3.571
赵文平，贾龙刚，路福平，刘夫锋*。基于β-内酰胺酶构建大肠杆菌体内Aβ42聚集抑制剂筛选体系, 食品与发酵工业, 2019, 45: 17-24.
Yihan Liu, Lin Huang, Mengying Shan, Jingcheng Sang, Yanzhen Li, longgang Jia, Nan Wang, Shuang Wang, Shulin Shao, Fufeng Liu*, Fuping Lu*. Enhancing the activity and thermostability of Streptomyces mobaraensis transglutaminase by directed evolution and molecular dynamics simulation, Biochemical Engineering Journal, 2019, 151: 107333. 2区， IF:3.371
Huitu Zhang&, Jingcheng Sang&, Ying Zhang&, Tongwei Sun, Huan Liu, Rong Yue, Jing Zhang, Haikuan Wang, Yujie Dai, Fuping Lu*, Fufeng Liu*. Rational design of a Yarrowia lipolytica derived lipase for improved thermostability, International Journal of Biological Macromolecules, 2019, 137: 1190-1198.
Longgang Jia, Ying Wang, Wei Wei, Wenping Zhao, Fuping Lu*, Fufeng Liu*. Vitamin B12 inhibits α-synuclein fibrillogenesis and protects against amyloid-induced cytotoxicity. Food & Function, 2019, 10, 2861-2870.
Longgang Jia#, Ying Wang#, Jingcheng Sang, Wei Cui, Wenping Zhao, Wei Wei, Beibei Chen, Fuping Lu*, Fufeng Liu*, Dihydromyricetin inhibits α-synuclein aggregation, disrupts the preformed fibrils and enhances cell viability, J Agric Food Chem, 2019, 67(14): 3946-3955.
Qingmei Sun#, Fufeng Liu#, Jingcheng Sang, Miaoman Lin, Jiale Ma, Xiao Xiao, Sicheng Yan, C. Benjamin Naman, Ning Wang, Shan He, Xiaojun Yan, Wei Cui*, Hongze Liang*. 9-Methylfascaplysin is a more potent Aβ aggregation inhibitor than the marine-derived alkaloid, fascaplysin, and produces nanomolar neuroprotective effects in SH-SY5Y Cells, Marine drugs, 2019, 17(2): 121.
李丽，刘夫锋*。淀粉样β蛋白质构象转换及其抑制的分子动力学模拟，生物加工过程，2019,17（1）：44-52。
Longgang Jia, Wenjuan Wang, Jingcheng Sang, Wei Wei, Wenping Zhao, Fuping Lu*, Fufeng Liu*. Amyloidogenicity and Cytotoxicity of a Recombinant C-terminal His6-tagged Aβ1-42. ACS Chemical Neuroscience. 2019, 10(3): 1251-1262.
Fufeng Liu٭, Wenjuan Wang, Jingcheng Sang, Longgang Jia, Fuping Lu. Hydroxylated Single Wall Carbon Nanotube Inhibits Aβ1-42 Fibrillogenesis, Disaggregates Preformed Fibrils, and Protects against Aβ42-Induced Cytotoxicity. ACS Chemical Neuroscience. 2019, 10(1): 588-598.
刘夫锋, 李丽*。抗体亲和肽配基的高通量筛选和理性设计。天津科技大学学报，2019, 1: 1-11.
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