研究领域
主要研究领域：分子酶工程（蛋白酶/抗体）、蛋白表达和高通量技术、系统生物学、生物传感
1)Protease/AntibodyEngineeringforIndustrialandTherapeuticApplications:focusingonproteaseengineeringforitsapplicationsinindustryandmedicine(acuteautoimmunediseases),scFVandFabengineeringofanti-TNF-αandanti-MMPsantibodiesfortherapeutics(Yeastsurface/Phagedisplaytechnologies,Structuremodeling,Directedevolution,High-throughputscreeningandsequencing,Biocatalysis,MassSpectrometry,Enzymekinetics,MachineLearning,etc.)
2)ProteinFolding&SecretionMechanismsinEukaryoticCells:exploringtheproteinfoldingandsecretionmechanismsineukaryoticcells,in-depthanalysisofproteininteractionsandmodificationsinyeastcells,andERdisorderassociatedhumandiseases(Systematicbiology,Biochemistry,PPI,High-throughputsequencing,Bioinformatics,etc.)
3)Proteins/ChemicalsProductioninMicrobialSystems:developingefficientmicrobialcellfactories(S.cerevisiae,P.pastoris,E.coli,B.subtilis,etc)usingsystemsbiology,syntheticbiology,andmetabolicengineeringforproteinsandchemicalsproduction(Strainengineering,Proteinfolding,Secretion,andCellsurfaceimmobilization,etc.)
近期论文
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1)Mei,M.,Li,J.,Wang,S.,Lee,B.,IversonB.L.,Zhang,G.,Ge,X.,*andYi,L.*,"FunctionalFabyeastsurfacedisplayofAdalimumabanditsvariantsagainstTNF-αthroughabi-directionalpromoterstrategy",Front.Bioeng.Biotechnol.,2019
2)Fan,X.,Li,X.,Zhou,Y.,Mei,M.,Peng,W.,Jiang,Z.,Yang,S.,IversonB.L.,Zhang,G.,*andYi,L.*,"Quantitativeanalysisofsubstratespecificitiesofthe3Cproteaseofhumanrhinovirusandtheexplorationofitssubstraterecognitionmechanisms",ACSChem.Biol.,2019
3)Zheng,Y.,Han,J.,Wang,B.,Hu,X.,Li,R.,Shen,W.,Ma,X.,Ma,L.,Yi,L.*,Yang,S.*,andPeng,W.*,"CharacterizationandrepurposingoftheendogenousTypeI-FCRISPR-CassystemofZymomonasmobilisforgenomeengineering",NucleicAcidsRes.,2019
4)Yang,Y.,Shen,W.,Li,R.,Huang,J.,Xiao,Y.,Wei,H.,Chou,Y.,Zhang,M.,Himmel,M.E.,Chen,S.,Yi,L.,Ma,L.,andYang,S.*,“ApplicationofZymomonasmobilisinbiotechnologybasedontoolsfromthesystembiologyera”,Biotechnol.Biofuels,2019Mar14;12:52
5)Lu,Z.,Yang,S.,Yuan,X.,Shi,Y.,Ou,Y.,Jiang,S.,Yi,L.,andZhang,G.*,"CRISPR-assistedmulti-dimensionalregulationforfine-tuninggeneexpressioninBacillussubtilis",2019,NucleicAcidsRes.,2019Apr23;47(7):e40.
6)Mei,M.,Zhai,C.,Li,X.Z.,Zhou,Y.,Peng,W.F.,Ma,L.,Wang,Q.H.,IversonB.L.,Zhang,G.M.*,andYi,L.*“Characterizationofaromaticresidue-controlledproteinretentionintheendoplasmicreticulumofSaccharomycescerevisiae”,J.Biol.Chem.,2017,Dec15;292(50):20707-20719
7)Mei,M.,Zhou,Y.,PengW.F.,Yu,C.,Ma,L.X.,Zhang,G.M.*,andYi,L.*“ApplicationofModifiedYeastSurfaceDisplayTechnologiesforNon-AntibodyProteinEngineering”,Microbiol.Res.,2017Mar;196:118-128
8)Li,Q.,#Yi,L.,#Hoi,K.H.,Marek,P.,Georgiou,G.*,andIverson,B.L.*“ProfilingProteaseSpecificity:CombiningYeastERSequestrationScreening(YESS)withNextGenerationSequencing”,ACSChem.Biol.,2017Feb17;12(2):510-518.(#:co-firstauthor.)
9)Song,H.T.,Gao,Y.,Yang,Y.M.,Xiao,W.J.,Liu,S.H.,Xia,W.C.,Liu,Z.L.,Yi,L.,andJiang,Z.B.*“Synergisticeffectofcellulaseandxylanaseduringhydrolysisofnaturallignocellulosicsubstrates”,Bioresour.Technol.,2016,219:710-5
10)Yi,L.*,Li,Q.,Taft,J.M.,Gebhard,M.C.,Iverson,B.L.*,andGeorgiou,G.*“YeastEndoplasmicReticulumSequestrationScreeningfortheEngineeringofProteasesfromLibrariesExpressedinYeast”,MethodsMol.Biol.2015,1319:81-93.
11)Li,Q.,Yi,L.,andMarek,P.“AntibodiesandTheirMultivalentConstructsforCancerTherapy”,ProteinPept.Lett.2014,21(10):1017-24
12)Yi,L.,Gebhard,M.C.,Li,Q.,Taft,J.M.,Georgiou,G.,andIverson,B.L.“EngineeringofTEVproteasevariantsbyyeastERsequestrationscreening(YESS)ofcombinatioriallibraries”,PNAS,2013,110(18):7229-34.
13)Ragsdale,S.W.,Yi,L.,etal.“Redox,HaemandCOinEnzymaticCatalysisandRegulation”,Biochem.Soc.Trans.,2012,40(3):501-7.
14)Ragsdale,S.W.,andYi,L.“Thiol/DisulfideRedoxSwitchesinTheRegulationofHemeBindingtoProteins”,Antioxid.RedoxSignal.,2011,14(6):1039-1047.
15)Yi,L.,Morgan,J.T.,andRagsdale,S.W.“IdentificationOfaThiol/DisulfideRedoxSwitchinTheHumanBKChannelThatControlsItsAffinityForHemeAndCO”,J.Biol.Chem.,2010,285(26):20117-20127.
16)Yi,L.,Jenkins,P.M.,Leichert,L.I.,Jakob,U.,Martens,J.R.,andRagsdale,S.W.“HemeRegulatoryMotifsinHemeOxygenase-2FormaThiol/DisulfideRedoxSwitchThatRespondstoTheCellularRedoxState”J.Biol.Chem.,2009,284,20556-20561.
17)Bianchetti,C.M.,Yi,L.,Ragsdale,S.W.,andPhilips,G.N.,Jr."ComparisonOfApo-AndHeme-BoundCrystalStructuresOfaTruncatedHumanHemeOxygenase-2",J.Biol.Chem.,2007,282,37624-37631.
18)Yi,L.,andRagsdale,S.W."EvidenceThatTheHemeRegulatoryMotifsinHemeOxygenase-2ServeAsaThiol/DisulfideRedoxSwitchRegulatingHemeBinding",J.Biol.Chem.,2007,282,20156-21067.
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