研究领域
生化分析及诊断
利用现代光谱及分子探针技术,研究建立生物大分子高灵敏度的分析检测方法,为生命科学研究及重大疾病的早期诊断提供分子水平信息。研究领域主要包括:免疫及DNA分析中纳米粒子探针的高灵敏度检测;基因单核苷酸多态性(SNP)分析;基因表达分析;microRNA高灵敏度分析及其生物功能的研究。
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Bai Jie, Zhao Yunjie, Wang Zhibin, Liu Chenghui*, Wang Yucong, Li Zhengping*. Dual-Readout Fluorescent Assay of Protein Kinase Activity by Using TiO2-Coated Magnetic Microspheres. Analytical Chemistry, 2013, 85, 4813-4821.
Yongqiang Cheng,* Qing Du, Liyong Wang, Hailian Jia, and Zhengping Li*,Fluorescently Cationic Conjugated Polymer as an Indicator of Ligase Chain Reaction for Sensitive and Homogeneous Detection of Single Nucleotide Polymorphism. Analytical Chemistry, 2012, 84, 3739−3744.
Cuiping Li, Zhengping Li,* Hongxia Jia and Jingli Yan, One-step ultrasensitive detection of microRNAs with loop-mediated isothermal amplification (LAMP). Chemical Communications, 2011, 47, 2595–2597.
Jiangyan Zhang, Zhengping Li,* Hui Wang, Yucong Wang, Hongxia Jia and Jingli Yan,Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe. Chemical Communications, 2011, 47, 9465–9467.
Hongxia Jia, Zhengping Li*, Chenghui Liu, Yongqiang Cheng, Ultrasensitive Detection of microRNAs with Exponentially Isothermal Amplification. Angewandte Chemie International Edition, 2010, 49, 5498-5501.
Yongqiang Cheng, Xian Zhang, Zhengping Li*, Xiaoxia Jiao, Yucong Wang, Yali Zhang, Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling Circle Amplification. Angewandte Chemie International Edition, 2009, 48, 3268-3272.