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Wang, F., Zhu, M., Song, Z., Zhu, Z., Lu, F.,* Qin, H.-M.* Reshaping the binding pocket of lysine hydroxylase for enhanced activity. ACS Catal. 2020, 10: 13946-13956
Zhu, Z., Gao, X., Song, Z., Li, C., Lu, F.*, Tanokura, M.*, Qin, H.-M.* Development of engineered ferredoxin reductase systems for the efficient hydroxylation of steroidal substrates. ACS Sustain. Chem. Eng. 2020, 8: 16720-16730;
Mao, S., Liu, X., Gao, X., Zhu, Z., Sun, D., Lu, F.*, Qin, H.-M.* Design of an efficient whole-cell biocatalyst for the production of hydroxyarginine based on a multi-enzyme cascade. Bioresource Technol. 2020, 318, 124261
Li, C., Wu, M., Gao, X., Zhu, Z., Li, Y., Lu, F.*, Qin, H.-M.* Efficient biosynthesis of 2'-fucosyllactose using an in vitro multienzyme cascade. J. Agric. Food Chem. 2020, 68: 10763-10771
Qin, H.-M., Gao, D., Zhu, M., Li, C., Zhu, Z., Wang, H., Liu, W.*, Tanokura, M.*, Lu, F.* Biochemical characterization and structural analysis of ulvan lyase from marine Alteromonas sp. reveals the basis for its salt tolerance. Int. J. Biol. Macromol. 2020,147, 1309-1317
Mao, S., Song, Z., Wu, M., Wang, X., Lu, F.*, Qin, H.-M.* Expression, purification, refolding and characterization of a neverland protein from Caenorhabditis elegans. Front. Bioeng. Biotechnol. 2020, doi: 10.3389/fbioe.2020.593041
Mao, S., Cheng, X., Zhu, Z., Chen, Y., Li, C., Zhu, M., Liu, X., Lu, F.*, Qin, H.-M.* Engineering a thermostable version of D-allulose 3-epimerase from Rhodopirellula baltica via site-directed mutagenesis based on B-factors analysis. Enzyme Microb. Technol. 2020, 132: 109441
Sun, D., Gao, D., Liu, X., Zhu, M., Li, C., Chen, Y., Zhu, Z., Lu, F.*, Qin, H.-M.* Redesign and engineering of a dioxygenase targeting biocatalytic synthesis of 5-hydroxyl leucine. Catal. Sci & Technol. 2019, 9: 1825-1834
Sun, D., Liu, X., Zhu, M., Chen, Y., Li, C., Cheng, X., Zhu, Z., Lu, F.*, Qin, H.-M.* Efficient biosynthesis of high-value succinic acid and 5-hydroxyleucine using a multienzyme cascade and whole-cell catalysis. J. Agric. Food Chem. 2019,67, 12502-12510
Zhu, Z., Gao, D., Li, C., Chen, Y., Zhu, M., Liu, X., Tanokura, M.*, Qin, H.-M.*, Lu, F. Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose. Microb. Cell Fact. 2019, 18:59
Mao, S., Guo, Q., Xu, P., Gao, D., Cheng, X., Zhu, M., Sun, D., Zhu, Z., Lu F.,* Qin, H.-M.* Biochemical and structural characterization of 3-ketosteroid-∆1-dehydrogenase, a candidate enzyme for efficient bioconversion of steroids. J. Chem. Technol. Biotechnol. 2019, 94: 309-316
Zhu, Z., Li, C., Liu, X., Gao, D., Wang, X., Tanokura, M.*, Qin, H.-M.*, and Lu, F.* Biochemical characterization and biocatalytic application of a novel D-tagatose 3-epimerase from Sinorhizobium sp. RSC Adv. 2019, 9: 2919-2927
Mao, S., Liu, Y., Yang, J. Ma, X., Zeng, F., Zhang, Z., Wang, S., Han, H., Qin, H.-M.*, Lu, F.* Cloning, expression and characterization of a novel fructosyltransferase from Aspergillus niger and its application in the synthesis of fructooligosaccharides. RSC Adv. 2019, 9: 23856-23863
Zhu, Z., Li, C., Cheng, X., Chen, Y., Zhu, M., Liu, X., Mao, S., Qin, H.-M.*, Lu, F.* Soluble expression, purification and biochemical characterization of a C-7 cholesterol dehydrogenase from Drosophila melanogaster. Steroids 2019, 152, 108495
Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Ojima, T., Tanokura, M. Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family. Chem. Commun. 2018, 54: 555-558
Mao, S., Wang, J.-W., Liu, F., Zhu, Z., Gao, D., Guo, Q., Xu, P., Ma, Z., Hou, Y., Cheng X., Sun, D., Lu, F.*, Qin, H.-M.* Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates. Microb. Cell Fact. 2018, 17:141
Mao, S., Li, K., Hou, Y., Liu, Y., Ji, S., Qin, H.-M.*, Lu, F.* Synergistic effects of components in deep eutectic solvents relieve toxicity and improve the performance of steroid biotransformation catalyzed by Arthrobacter simplex, J. Chem. Technol. Biotechnol. 2018, 93: 2729–2736
Qin, H.-M., Xu, P., Guo, Q., Cheng, X., Gao, D., Sun, D., Zhu, Z., Lu, F., Biochemical characterization of a novel ulvan lyase from Pseudoalteromonas sp. strain PLSV. RSC Adv. 2018, 8: 2610-2615
Mao, S., Liu, Y., Hou, Y., Ma, X., Yang, J., Han, H., Wu, J., Jia, L., Qin, H.*, and Lu, F.* Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892, RSC Adv. 2018, 8: 39897-39901
Sun, D., Gao, D., Xu, P., Guo, Q., Zhu, Z., Cheng, X., Bai, S., Qin, H.-M.*, Lu, F.* A novel L-leucine 5-hydroxylase from Nostoc piscinale unravels unexpected sulfoxidation activity toward L-methionine, Protein Expres. Purif. 2018, 149, 1-6
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